In order to effectively reduce the psychological anxiety of subway passengers in the fire state and improve the effect of fire emergency management, the fire anxiety status of passengers of different genders was counted, based on the fire state-trait anxiety questionnaire. The questionnaire and mathematical statistics were used to discuss the influence of different time periods after the intervention of on-site drills on the psychological anxiety level of passengers. Results: Before the field exercise, the overall fire anxiety level of the sample was in the normal range ; After on-site exercise, female fire anxiety level decreased, but still did not eliminate anxiety, and the effect of male education is not obvious ; After one hour of on-site training, the overall scores of fire anxiety of the samples were reduced; One day after the on-site exercise, women’s psychological anxiety scores began to improve, and men’s psychological anxiety continued to improve. Conclusion: On the whole, on-site drills are effective. After one day of on-site drills, the intervention effect basically disappears. However, on-site drills have a significant effect on eliminating the tension in the face of subway fires, and there are significant differences in these aspects of characteristics between people of different genders in the face of subway fires.