A specular-reflection photonic nanojet (s-PNJ) is a specific type of optical near-field subwavelength spatial localization originated from the constructive interference of direct and backward propagated optical waves focused by a transparent dielectric microparticle located near a flat reflecting mirror. The unique property of s-PNJ is reported for maintaining its spatial localization and high intensity when using microparticles with high refractive index contrast when a regular photonic nanojet is not formed. The physical principles of obtaining subwavelength optical focus in the specular-reflection mode of a PNJ are numerically studied and a comparative analysis of jet parameters obtained by the traditional schemes without and with reflection is carried out. Based on the s-PNJ, the physical concept of an optical tweezer integrated into the microfluidic device is proposed provided by the calculations of optical trapping forces of the trial gold nanosphere. Importantly, such an optical trap shows twice as high stability to Brownian motion of the captured nano-bead as compared to the conventional nanojet-based traps and can be relatively easy implemented.