Subtropical Asia Research Articles

Small carnivores contribute surrogate seed dispersers for a megafaunal-fruited liana in subtropical Asia

The megafaunal seed dispersal hypothesis posits that mega-fruits co-evolved for seed dispersal by extremely large mammals, such as elephant-like gomphotheres, which became extinct during the Pleistocene. Nevertheless, various plants in subtropical Asia still produce oversized fruits as a seed dispersal syndrome anachronism. Here, we investigated how a mega-fruited perennial woody liana, Akebia trifoliata achieves the consumption of its large, sweet, edible fruits to disperse its seeds in a subtropical region on China where the mega frugivores are absent nowadays. We hypothesized that some other surrogate species may be fulfilling this dispersal function. From 2926 fecal samples from 13 species of mammal and various unidentified birds, collected over two years, we identified A. trifoliata seeds in only 13 feces from the masked palm civet (Paguma larvata). Our systematic sampling allowed us to eliminate any role being played by all other plausible dispersal mutualists; not least, these fruits rarely fall to the ground and deteriorate quickly, and so must be picked from the vine by species able to reach 2–5m above the ground. Of 338 masked palm civet feces examined during the August to September fruiting season only 3.85% (frequency of occurrence) contained A. trifoliata seeds, although when seeds were present, they were abundant (c. 600/feces). All seeds passing through the gut of masked palm civets were intact. Moreover, masked palm civets mainly dispersed A. trifoliata seeds in degraded habitats (i.e., the logged, and selectively logged forests). These results showed that although masked palm civets dispersed A. trifoliata seeds in low proportion, they could play a surrogate seed dispersal role for this mega-fruit vine species, especially in degraded forests. Our findings highlight that, in subtopical Asia, small frugivorous carnivores can act as surrogate seed dispersers for oversized fruit plants, advancing knowledge on how past species interactions and defaunation underlie modern plant distributions.

Multi-epitope vaccine candidate design for dengue virus.

Dengue Fever (DF) is a vector-borne neglected viral disease with a high burden in the sub-tropics of Asia and Africa. Aedes aegypti is responsible for 90% of cases in the global burden of disease. The primary goal of the treatment is to eliminate the virus from the bloodstream of affected individuals. A successful dengue vaccine must elicit both neutralizing antibodies and cell-mediated immunity and there is no vaccine to date to prevent DF. A multi-epitope vaccine composed of a series of or overlapping peptides is, therefore, an ideal approach for the prevention and treatment of pathogenic organisms. An immunoinformatics approach was employed to design a theoretical multi-epitope vaccine candidate. This vaccine candidate consists of linear B-cell epitope, TH cells epitope and CTL of reported potential vaccine candidates. These epitopes were linked together with suitable linkers and adjuvant at the N terminal and C terminal. The 3D Structure of the vaccine was modeled, refined and validated using computational tools. Protein-protein docking of vaccine candidates with TLR3 protein results in efficient binding. Immune stimulation of vaccine candidates predicted high levels of IgG and IgM. This candidate vaccine should be validated experimentally using suitable in-vivo and in-vitro studies to use in dengue fever virus elimination programmes.

The ryanodine receptor mutation I4728M confers moderate-level resistance to diamide insecticides in Spodoptera litura.

The common cutworm, Spodoptera litura (Fabricius), is one of the most widespread and destructive polyphagous pests in tropical and subtropical Asia. S. litura has evolved resistance to different insecticides, including diamide insecticides. Here, we identified a ryanodine receptor (RyR) mutation (I4728M) associated with target site resistance to diamides in a field-collected population of S. litura. The contribution of this mutation to diamide resistance was investigated through establishing a near-isogenic resistant strain of S. litura. The ND21 population of S. litura, collected from Ningde, Fujian province of China in 2021, exhibited 130.6-fold resistance to chlorantraniliprole compared to the susceptible NJ-S strain. S. litura RyR mutation I4728M, corresponding to Plutella xylostella RyR I4790M, was identified in the ND21 population. SlRyR I4728M mutation of ND21 was introgressed into a susceptible background strain (NJ-S) with marker-assisted backcrossing. The introgressed strain named ND21-R, which was homozygous for the mutant 4728M allele, shared about 94% of the genetic background with the NJ-S strain. ND21-R strain showed moderate levels of resistance to two anthranilic diamides (19.1-fold to chlorantraniliprole, 19.7-fold to cyantraniliprole) and the phthalic diamide flubendiamide (23.4-fold). Genetic analysis showed that chlorantraniliprole resistance was autosomal, incompletely recessive and tightly linked with SlRyR I4728M mutation in the introgressed ND21-R strain of S. litura. Identification of the I4728M mutation and its contribution to diamide resistance in S. litura will help develop allelic discrimination assays for resistance monitoring and guide resistance management practices for diamides in S. litura. © 2023 Society of Chemical Industry.

Spatial pattern assessment of dengue fever risk in subtropical urban environments: The case of Hong Kong

Dengue fever, a mosquito-borne fatal disease, brings a huge health burden in tropical regions. With global warming, rapid urbanization and the expansion of mosquitoes, dengue fever is expected to spread to many subtropical regions, leading to increased potential health risks on local populations. So far, limited studies assessed the dengue fever risk spatially for subtropical non-endemic regions hindering the development of related public health management. Therefore, we proposed a spatial hazard-exposure-vulnerability assessment framework for mapping the dengue fever risk in Hong Kong. Firstly, the spatial distribution of the habitat suitability for Aedes albopictus, the mosquito proxy for the dengue fever hazard, was predicted using a species distribution model (e.g., MaxEnt) relying on a list of variables related to local climate, urban morphology, and landscape metrics. Secondly, the spatial autocorrelation between high dengue hazard and high human population exposure in urban areas was measured. Finally, the dengue fever risk was assessed at community scale by integrating the results of vulnerability analysis basing on census data. This approach allowed the identification of 17 high-risk spots within Hong Kong. The landscape metrics about land utilities and vegetations, and urban morphological characteristics are the influential factors on the spatial distribution of dengue vector. In addition, the underlying factors behind each hot spot were investigated, and specific suggestions for dengue prevention were proposed accordingly. The findings provide a useful reference for developing local dengue fever risk prevention measures, with the proposed method easily exportable to other high-density cities within subtropical Asia and elsewhere.

The invasive Spodoptera frugiperda (J.E. Smith) has displaced Ostrinia furnacalis (Guenée) as the dominant maize pest in the border area of southwestern China.

The invasive fall armyworm (FAW) Spodoptera frugiperda (Lepidoptera: Noctuidae) has widely colonized the tropics and subtropics of Asia. However, the impact on the succession of the Asiatic corn borer (ACB) Ostrinia furnacalis (Lepidoptera: Pyralidae), a perennial dominant stemborer of maize in these areas, remains elusive. Here we analyzed the predation relationship, mimicked population competition, and surveyed the pest populations in the border area of Yunnan (southwestern China). Laboratory assays revealed that the 2nd to 6th instar larvae of FAW preyed on ACB, and only the 4th and 5th instar larvae of ACB preyed on FAW (1st instar larvae only, 50% predation rate). The 6th instar FAW preyed on the 1st to 5th instar ACB with a theoretical maximum of 14.5-58.8 ACB individuals (per maize leaf) and 4.8-25.6 individuals (per tassel). When maize plants were infested with eggs of either FAW or ACB in field cage trials, maize damage was 77.6% and 50.6%, respectively, compared with 77.9% and 2.8% upon co-infestation. In field surveys conducted in 2019-2021, FAW density was significantly greater than that of ACB, which took a great impact on maize growth. Our findings indicate that FAW can outcompete ACB at both the individual and population levels, which may result in FAW becoming the dominant pest. These results provide a scientific basis for further analysis of the mechanism by which FAW invades new agricultural areas and offers early-warning strategies for pest management. © 2023 Society of Chemical Industry.

Phylogenetic relationships and biogeography of Asia Callicarpa (Lamiaceae), with consideration of a long-distance dispersal across the Pacific Ocean -insights into divergence modes of pantropical flora.

There are about 140 species of Callicarpa L. 1753 (Lamiaceae), with more species richness in tropical to subtropical Asia and the New World. The genus might provide an insight into the amphi-Pacific disjunction pattern of tropical and subtropical vegetation. This study has greatly improved the phylogenetic underpinning for Callicarpa, derived from more inclusive taxonomic samplings, and employing data on both two-nuclear and eight-chloroplast regions. To address time and patterns of diversification in Callicarpa, we conducted divergence time and biogeographic analyses, and inferred shifts in the distribution areas across the phylogenetic clades. Our phylogenetic results show that Callicarpa is monophyletic with respect to the groups considered, and eight well-supported primary clades were discerned in the combined analyses. Our estimates indicated that the crown group of Callicarpa originates around the Late-Eocene (ca. 36.23 Ma) and diversification within most clades is concentrated in the Miocene and continued to the Pleistocene. In addition, our biogeographic analyses suggested that the probable ancestor of the Callicarpa crown clade originated in East Asia and Southeast Asia. Multiple dispersal and vicariance events contributed to the current distribution of the taxa. Furthermore, this genus expanded eastward out of East and Southeast Asia to the New World by long-distance dispersal, which inspired us to better understand the amphi-Pacific disjunct distribution.

Molecular Insights into Abiotic Stresses in Mango.

Mango (Mangifera indica L.) is one of the most economically important fruit crops across the world, mainly in the tropics and subtropics of Asia, Africa, and Central and South America. Abiotic stresses are the prominent hindrance that can adversely affect the growth, development, and significant yield loss of mango trees. Understanding the molecular physiological mechanisms underlying abiotic stress responses in mango is highly intricate. Therefore, to gain insights into the molecular basis and to alleviate the abiotic stress responses to enhance the yield in the mere future, the use of high-throughput frontier approaches should be tied along with the baseline investigations. Taking these gaps into account, this comprehensive review mainly speculates to provide detailed mechanisms and impacts on physiological and biochemical alterations in mango under abiotic stress responses. In addition, the review emphasizes the promising omics approaches in unraveling the candidate genes and transcription factors (TFs) responsible for abiotic stresses. Furthermore, this review also summarizes the role of different types of biostimulants in improving the abiotic stress responses in mango. These studies can be undertaken to recognize the roadblocks and avenues for enhancing abiotic stress tolerance in mango cultivars. Potential investigations pointed out the implementation of powerful and essential tools to uncover novel insights and approaches to integrate the existing literature and advancements to decipher the abiotic stress mechanisms in mango. Furthermore, this review serves as a notable pioneer for researchers working on mango stress physiology using integrative approaches.

Remotely sensed environmental data as ecological proxies for ground‐dwelling ant diversity along a subtropical forest succession gradient

Abstract Early naturalists such as Humboldt observed that changes in topography and anthropogenic disturbances influenced vegetation structure and the composition of animal communities. This holistic view of community assembly continues to shape conservation and restoration strategies in an era of landscape degradation and biodiversity loss. Today, remote sensing affords ecologists the tools for obtaining rapid and precise measures of topography, disturbance history and vegetation structure. Nonetheless, the capacity of such measures to predict the structure of diverse and functionally important insect communities has not been fully explored. We sampled ground‐dwelling ant assemblages with pitfall traps along a successional gradient (15 grasslands, 21 shrublands and 44 forests) in subtropical Asia, and measured the taxonomic (TD) and functional diversity (FD). We used airborne Light Detection and Ranging (LiDAR) and aerial photography—to measure topography, anthropogenic‐fire history and vegetation structure at each site. Using structural equation models, we tested the hypothesis that vegetation structure mediated the effects of topography and anthropogenic‐fire history on ant assemblage TD and FD, with stronger effects on the latter. We found that low elevation and anthropogenic‐fire history promoted ant TD, and by mediating vegetation structure, these factors further controlled ant FD. Specifically, assemblages of ant species occupying more similar niches—as indicated by their lower FD—were found in secondary forests that had more structurally homogeneous vegetation. These sites also had low insolation and high water moisture content, and were not recently burned as revealed by LiDAR‐derived metrics and aerial images. Furthermore, remotely sensed vegetation structures were closely associated with individual ant traits, such as body size and eye length, which reflect species' preferences for habitat structure. Synthesis. Our study uncovers the interactive effects of topography, disturbance history and vegetation structure in determining the TD and FD of ant assemblages in subtropical landscapes. Moreover, it demonstrates that remote sensed data can be leveraged to efficiently elucidate the complex effects of environmental change and disturbances on vegetation structure and consequently insect biodiversity, representing ecological proxies to refine ground investigation plans and support appropriate conservation and restoration measures for degraded landscapes.

Future Drying Subtropical East Asia in Winter: Mechanism and Observational Constraint

Abstract Coupled climate models project robust wintertime wetting trend over midlatitude East Asia under global warming scenarios, but the projected change in precipitation shows large intermodel uncertainty over subtropical East Asia from southern China to southwestern Japan. Based on an ensemble of climate models participating in CMIP6, this study shows that the weakened southern branch westerly jet (SWJ) on the southern side of Tibetan Plateau (TP) plays a key role in suppressing subtropical East Asian precipitation. The SWJ is deflected into southwesterly wind on the southeastern side of TP, bringing ascent and precipitation to subtropical East Asia primarily through isentropic gliding. As a result of the poleward and upward shift of the planetary-scale westerly jet under global warming, the SWJ becomes weaker and it acts to suppress subtropical East Asian precipitation by weakening the southwesterly wind and ascent. The SWJ–precipitation linkage also exists on interannual time scales, but the sensitivity of precipitation to interannual SWJ variability is systematically underestimated by the models compared with observation. The combined effects of the change in SWJ strength and the sensitivity of precipitation to SWJ explain about 40% of the intermodel spread of the projected precipitation changes. Observational constraint on the SWJ–precipitation relationship amplifies the projected drying trend and narrows the intermodel spread. It shows that the regional-averaged precipitation over subtropical East Asia decreases by 3.3% per degree of warming, and the amplitude of precipitation reduction over subtropical East Asia (southern China) is about 1.4 (3.4) times the raw projection.

The role of transient eddies in the intraseasonal reversal of East Asian winter air temperature anomalies

The intraseasonal reversal of wintertime surface air temperature (SAT) anomalies over East Asia (15°-55°N, 80°-130°E) is becoming a matter of concern. Thus, the predominant features of the reversal mode of SAT anomalies and its relevant dynamical mechanism associated with transient eddies (TEs) are studied by using the Multivariate Empirical Orthogonal Function (MV-EOF) analysis method and the geopotential tendency equation. Results show that the third MV-EOF mode well depicts a colder (warmer) DJ with a warmer (colder) February and is closely linked to an out-of-phase variation in both East Asian Polar Front Jet (EAPJ) and East Asian Subtropical Jet (EASJ). It is found that synoptic-scale (2.5-8d) and low-frequency (10-90d) TE activities over Eurasia play important roles in the intraseasonal reversal in SAT anomalies by adjusting time-mean flows. A cold DJ in East Asia dynamically corresponds to a strengthened EASJ and weakened EAPJ, while the TE forcing dominated by low-frequency TE vorticity forcing and synoptic-scale TE heating forcing tends to weaken the EASJ and reinforce the EAPJ, unfavorable to the maintenance of cold anomalies over East Asia. However, the characteristics of both synoptic-scale and low-frequency TE activities in February are significantly different from that in DJ. By comparison, the TE forcing is dominated by synoptic-scale TE vorticity forcing and low-frequency TE heating forcing, which makes great contribution to the weakening of EASJ and the enhancement and maintenance of EAPJ, conducive to a weaker East Asian winter monsoon and eventually to a warmer East Asia.

Gymnadenia conopsea (L.) R. Br.: Comprehensive review of propagation and breeding, traditional uses, chemical composition, pharmacology, quality control, and processing.

Gymnadenia conopsea, a perennial herbaceous flowering plant that belongs to the family of Orchidaceae, sporadic distributed in the altitudes of 200-4700m across northern Europe and, temperate and subtropical Asia region. The dried tubers of G. conopsea have been used to treat cough, asthma, and their syndromes, and also as a tonic in China and surrounding countries for a long history. G. conopsea is often processed deeply processed before use to enhance its efficacy. In recent years, because of its remarkable pharmacological activity and health care function, G. conopsea has been used more and more widely. Due to its extensive application and bad growth environment, the wild distribution of G. conopsea is decreasing and it has been listed as an endangered plant. This review aims to summarize the propagation and breeding, traditional uses, chemical composition, pharmacology, quality control, and processing of G. conopsea. Moreover, it also provides suggestions for the future high-value utilization of G. conopsea. A literature search on Gymnadenia genus and G. conopsea was performed using scientific databases including SciFinder, ACS, Web of Science, Springer, ScienceDirect, PubMed, and CNKI. Information was also collected from classic books of Chinese herbal medicine, official websites, Ph.D. and M.Sc. Dissertations, and so on. Structures of chemical compounds were drawn by ChemDraw software. As of submission date of this manuscript, total 170 natural compounds have been isolated and characterized from G. conopsea, and all of the compounds were isolated from the tubers. The isolated compounds including benzylester glucosides, dihydrostilbenes, phenanthrenes, phenolic compounds, alkaloids, polysaccharide, lignans, flavones, triterpenoids, steroids, and other compounds. Some of these compounds and active extracts exhibited a wide range of pharmacological activities, in which, the tonifying, anti-fatigue, anti-oxidant, anti-viral, sedative and hypnotic activities are consistent with the traditional uses for the treatment of diseases. In addition, a variety of new pharmacological activities, such as preventing and treating gastric ulcers, immunoregulatory, anti-hyperlipidemia, anti-anaphylaxis, anti-silicosis, anti-cancer and neuroprotective activities have also been reported. However, the bioactive compounds responsible for most of the above pharmacological effects have not been well summarised till now. In this manuscript, analysis, speculation and summary of compounds that responsible for pharmacological effects were conducted. The chemical constituents and pharmacological activities studies of G. conopsea extract have been summarised in this context, the isolated compounds responsible for the pharmacological activities were also analyzed and deduced according to the publications, all above led to suggestions for the future high-value utilization of G. conopsea.

Predicting the range expansion of invasive alien grasses under climate change in the Neotropics

A diverse group of invasive grasses from tropical and subtropical Africa and Asia has spread throughout the Neotropics over the last decades. Despite their strong ecological impact, current and future distribution patterns of these grasses in the region according to climate change is poorly investigated. We chose ten high potential invasive grass species and used ecological niche modeling to project their geographic distribution within the Neotropics under four climate change scenarios (current, SSP1-2.6, SSP3-7.0 and SSP5-8.5 for 2100). Current climatically suitable areas for these invasive species were estimated to account for 51.3% of the Neotropics. Projections of future climatically suitable areas ranged between 47.0% and 57.6%, depending on the climate scenario. Range retractions are projected for Melinis repens and Urochloa decumbens regardless of the SSP scenario, while Arundo donax, Hyparrhenia rufa and Melinis minutiflora are expected to expand their range in all SSP scenarios. Currently, these ten invasive species have suitable areas that greatly overlap in dry regions of the Neotropics, mainly in the savannas of Central Brazil and Central America. However, a reduction in species overlap and a geographical expansion towards wetter regions is expected under the SSP1 and SSP3 scenarios, and towards drier regions under the SSP5 scenario.

Field Assessment of Strip-Tillage for Summer Rice Cultivation in Bangladesh

Farmers in the tropics and subtropics of Asia usually transplant rice seedlings in puddled soils which makes crop establishment and weed control easier. But rice can be grown successfully by planting into non-puddled soils after a single shallow pass of strip tillage. This method was globally reported to produce the same yield of rice as traditional puddle farming and saved labor, fuel and water, too. With this aim to asses the effect of strip tillage in the productivity of rice in Bangladesh, a trial was done at a farmer's field in the Gauriopur area of Mymensingh district in Bangladesh from July to December 2014. The productivity of four summer rice varieties, BR11, BRRI dhan46, BRRI dhan52, and BINA Dhan-7, was compared under strip tillage (ST) vs. conventional tillage (CT). The CT had two main ploughings done by a two-wheel tractor, and the ST was done in a single pass by a Versatile Multi-crop Planter. The interactive results of study showed that, except for the number of effective tillers per m2, grain yield, and BCR, none of the other parameters changed significantly between the treatments. The highest BCR profit came from BR 11 grown under ST, followed by BRRI dhan52, BRRI dhan46, and BINA Dhan-7 grown under ST. The lowest BCR was measured from BINA Dhan-7 grown under CT. This could be because the highest and lowest amount of effective and sterile tillers and the maximum grain yield were seen in the respective treatments. The ST had a 26% higher BCR than the CT, which could have been due to the 3% higher grain yield that came from having 6% more effective tillers and 42% lower sterile tillers per m2 area. In the end, we could say that ST is a more profitable way to grow rice than CT, and BR 11 is the best of the other three types.

Axenic in vitro cultivation and genome diploidization of the moss Vesicularia montagnei for horticulture utilization.

Mosses are widely used in the establishment of greenery. However, little research has been conducted to choose a suitable species or improve their performance for this application. In our study, we examined Vesicularia montagnei (V. montagnei), a robust moss that is widely distributed in temperate, subtropical, and tropical Asia with varying environmental conditions. Axenic cultivation system of V. montagnei was developed on modified BCD medium, which enabled its propagation and multiplication in vitro. In this axenic cultivation environment, several diploid V. montagnei lines with enhancement of rhizoid system were generated through artificial induction of diploidization. Transcriptomic analysis revealed that several genes responsible for jasmonic acid (JA) biosynthesis and signaling showed significant higher expression levels in the diploid lines compared to the wild type. These results are consistent with the increasement of JA content in the diploid lines. Our establishment of the axenic cultivation method may provide useful information for further study of other Vesicularia species. The diploid V. montagnei lines with improved rhizoid system may hold promising potential for greenery applications. Additionally, our study sheds light on the biosynthesis and functions of JA in the early landed plants.

Near-global distributions of overshooting tops derived from Terra and Aqua MODIS observations

Abstract. Overshooting cloud tops (OTs) form in deep convective storms when strong updrafts overshoot the tropopause. An OT is a well-known indicator of convective updrafts and severe weather conditions. Here, we develop an OT detection algorithm using thermal infrared (IR) channels and apply this algorithm to about 20 years' worth of MODIS data from both Terra and Aqua satellites to form an extensive, near-global climatology of OT occurrences. The algorithm is based on a logistic model which is trained using A-Train observations. We demonstrate that the overall accuracy of our approach is about 0.9 when the probability of the OT candidates is larger than 0.9. The OT climatology reveals a pattern that follows the climatology of deep convection and shallow convection over the midlatitude oceans during winter cold-air outbreaks. OTs appear most frequently over the Intertropical Convergence Zone (ITCZ), central and southeastern North America, tropical and subtropical South America, southeastern and southern Asia, tropical and subtropical Africa, and northern middle–high latitudes. OT spatial distributions show strong seasonal and diurnal variabilities. Seasonal OT variations shift with large-scale climate systems such as the ITCZ and local monsoonal systems, including the South Asian monsoon, North American monsoon, and West African monsoon. OT diurnal variations agree with the known diurnal cycle of convection. Maximum OT occurrences are in the afternoon over most land areas and around midnight over ocean, and the OT diurnal cycle is stronger and more varied over land than over ocean. OTs over land are usually colder than over ocean, except at around 10:30 LT (Equator-crossing time). The top 10 coldest OTs from both Terra and Aqua mostly occur over land and at night. This study provides OT climatology for the first time, as derived from 2 decades of MODIS data, that represents the longest and stable satellite records.

Reconstructing 34 Years of Fire History in the Wet, Subtropical Vegetation of Hong Kong Using Landsat

Burn-area products from remote sensing provide the backbone for research in fire ecology, management, and modelling. Landsat imagery could be used to create an accurate burn-area map time series at ecologically relevant spatial resolutions. However, the low temporal resolution of Landsat has limited its development in wet tropical and subtropical regions due to high cloud cover and rapid burn-area revegetation. Here, we describe a 34-year Landsat-based burn-area product for wet, subtropical Hong Kong. We overcame technical obstacles by adopting a new LTS fire burn-area detection pipeline that (1) Automatically uniformized Landsat scenes by weighted histogram matching; (2) Estimated pixel resemblance to burn areas based on a random forest model trained on the number of days between the fire event and the date of burn-area detection; (3) Iteratively merged features created by thresholding burn-area resemblance to generate burn-area polygons with detection dates; and (4) Estimated the burn severity of burn-area pixels using a time-series compatible approach. When validated with government fire records, we found that the LTS fire product carried a low area of omission (11%) compared with existing burn-area products, such as GABAM (49%), MCD64A1 (72%), and FireCCI51 (96%) while effectively controlling commission errors. Temporally, the LTS fire pipeline dated 76.9% of burn-area polygons within two months of the actual fire event. The product represents the first Landsat-based burn-area product in wet tropical and subtropical Asia that covers the entire time series. We believe that burn-area products generated from algorithms like LTS fire will effectively bridge the gap between remote sensing and field-based studies on wet tropical and subtropical fire ecology.

Molecular Detection and Genetic Characterization of Zoonotic Hookworm in Semi-Domesticated Cats Residing in Monasteries in Bangkok, Thailand.

Hookworms are the most common parasitic nematodes in the genus of Ancylostoma that infect both humans and animals in subtropical and tropical South East Asia. The common hookworm species in cats is Ancylostoma ceylanicum which is emerging in Thailand. However, the genetic characterization of hookworms in cats is outdated and insufficiently studied in Thailand. We aimed to investigate the prevalence, risk factors and genetic characterization of hookworm infection in semi-domesticated temple cats in Bangkok, Thailand. A total of 500 temple cat fecal samples were collected from 43 monasteries in 24 districts of Bangkok, Thailand. Polymerase Chain Reaction (PCR) was performed by amplifying the internal transcribed spacer (ITS) gene and mitochondrial cytochrome oxidase c subunit I (cox 1) gene. The infection prevalence of hookworm in temple cats was 13.2% (66/500). The highest prevalence was 34.6% in the Bang Khun Thian district, which is located in a suburban area. The risk factor analysis revealed that cats older than one year (OR 2.4, 95% CI 1.1-5.5, p < 0.05), lack of veterinary attention (OR 2.9, 95% CI 1.7-4.9, p < 0.001) and Bangkok zone (suburban vs. inner city; OR 2.9, 95% CI 1.6-5.4, p < 0.001) were significantly increasing hookworm infection risk. All hookworm positive samples were identified as A. ceylanicum by ITS gene. Moreover, genetic characterization of cox 1 gene in A. ceylanicum isolates indicated a mix of isolates from humans, cats and dogs. The findings show that temple cats can act as a potential source of zoonotic hookworm parasites for the human and animal population in Bangkok, Thailand. Therefore, appropriate control measures for hookworms in semi-domesticated temple cats as well as prevention measures for hookworms in pet cats and humans should be promoted.

Molecular phylogeny and historical biogeography of Cyclommatus stag beetles (Coleoptera: Lucanidae): Insights into their evolution and diversification in tropical and subtropical Asia

Cyclommatus stag beetles (Coleoptera, Lucanidae) are very interesting insects, because of their striking allometry (mandibles can be longer that the whole body in large males of some species) and sexual dimorphism. They mainly inhabit tropical and subtropical forests in Asia. To date, there has been no molecular phylogenetic research on how these stag beetles evolved and diversified. In this study, we constructed the first phylogenetic relationship for Cyclommatus using multi-locus datasets. Analyses showed that Cyclommatus is monophyletic, being subdivided into two well-supported clades (A and B). The clade A includes the island species from Southeast Asia, and the clade B is formed by the continental species. The divergent time estimates showed these beetles split from the outgroup around 43.10 million years ago (Mya) in the late Eocene, divided during the late Oligocene (around 24.90 Mya) and diversified further during the early and middle Miocene (around 18.19 Mya, around 15.17 Mya). RASP analysis suggested that these beetles likely originated in the Philippine archipelago, then dispersed to the other Southeast Asian archipelagoes, Indochina Peninsula, Southeast Himalayas, and Southern China. Moreover, relatively large genetic distance and stable morphological variations signified that the two clades reach the level of inter-generic differences, i.e., the current Cyclommatus should be separated in two genera: Cyclommatus Parry, 1863 including the clade A species, and Cyclommatinus Didier, 1927 covering the clade B species. In addition, the evidence we generated indicated these beetles’ diversification was promoted probably by both long-distance dispersal and colonization, supporting an “Upstream” colonization hypothesis. Our study provides insights into the classification, genetics and evolution of stag beetles in the Oriental region.

A new species of Amphorotia (Bacillariophyta) from a freshwater section of the Pearl River with comments on associated species and biogeography of the genus

Based on a review of material previously studied, and new water samples, species of the genus <i>Amphorotia</i> were investigated with respect to their morphology and geographical distribution, especially in subtropical Asian coastal areas. Six <i>Amphorotia</i> species from China are investigated. They all possess amphoroid frustule symmetry, presence of spines and, when detected, a single plastid. These features as well as other details of the frustules were documented with light and scanning electron microscopy. Five of the taxa, <i>A. reimerii</i>,<i> A. mokonensis</i>,<i> A. baicalensis</i>,<i> A. zhujiangensis</i>, and <i>A. curvata</i> are newly recorded as fossil specimens in the Jianghan Plain. Further comments on the taxonomy and morphological variation of several known species are given. One taxon, <i>A. liangyanlingii</i> sp. nov. was discovered from freshwater samples in the Pearl River. This new species is most similar to<i> A. zhujiangensis</i> Wu, Williams et Flower when observed in LM, but can be distinguished by ultrastructural details when viewed with the scanning electron microscope (SEM). Taxonomical studies of species in the genus <i>Amphorotia</i> are considered together with their biogeography and ecology.

A Diagnostic Study of the Influence of Early Spring Soil Moisture in Southeastern China on Interannual Variability of the East Asian Subtropical Summer Monsoon Onset

A Diagnostic Study of the Influence of Early Spring Soil Moisture in Southeastern China on Interannual Variability of the East Asian Subtropical Summer Monsoon Onset