Subtropical River Research Articles

Variations of trophic structure and niche space in fish community along a highly regulated subtropical large river.

The trophic interactions between consumers and resources play a vital role in the stability of communities. In river systems, fragmentation of natural habitats and environmental changes alters the energy basis and community composition, consequently leading to variations in the community's trophic structure and niche space. However, our understanding of how the trophic structure responds to environmental changes is still very limited. Here, based on stable isotope data, we explored and compared trophic positions (TPs), community‐wide trophic metrics, and isotope niche space of fish communities in three reaches with different hydrogeomorphic conditions along a highly regulated subtropical river over three seasons. The community trophic structure and niche space showed notable spatiotemporal variations. Overall, the downstream reach had lower TPs, trophic diversity but higher trophic redundancy. The middle reach occupied a wider isotope niche space than other reaches, with the largest niche size during autumn. Furthermore, the niche overlap was relatively high in winter between reaches and in the downstream between seasons. The results implied a homogenization of feeding functional groups and energy flow pathways of species in the downstream community associated with the change of energy source and stability of hydrological conditions. The relationship between trophic structure and environmental factors suggested that the dam‐induced alteration in hydrological‐related aspects may drive the changes in the functional group composition, together with changes in energy basis, resulting in differences in the trophic structure of the community. The results of the present study deepen our understanding of how ecosystem functions respond to disturbance, thus contributing to improved ability to conserve river ecosystems.

Different responses of incidence-weighted and abundance-weighted multiple facets of macroinvertebrate beta diversity to urbanization in a subtropical river system

• Beta diversity weighted by incidence vs abundance data varied differently along urbanization gradient. • Richness difference contributed more to beta diversity weighted by abundance than incidence data. • Stochasticity was more dominant in shaping beta diversity weighted by abundance than incidence data. • Homogenization studies should consider the roles of rare and abundant taxa. Urbanization is one of the major drivers of biotic homogenization (i.e., decrease in beta diversity) in freshwater systems. However, only a few studies have simultaneously examined how urbanization affects multiple facets (i.e., taxonomic, functional and phylogenetic) of beta diversity and its underlying ecological drivers in urban river macroinvertebrates. Here, we distinguished the patterns and ecological mechanisms of multiple facets of macroinvertebrate beta diversity weighted by incidence and abundance data in a subtropical river system with a distinct urbanization gradient. We also investigated how total beta diversity patterns stem from replacement versus richness difference among sites. Our results showed that taxonomic and phylogenetic beta diversities weighted by incidence data were primarily driven by replacement of taxa, whereas the richness difference contributed more to multiple facets of beta diversity based on abundance data. Furthermore, multiple facets of beta diversity decreased with urbanization for both incidence-weighted and abundance-weighted data, but the former showed more substantial decreases. Both replacement and richness difference components contributed roughly equally to the decline of incidence-weighted beta diversity. In contrast, the losses of abundance-weighted beta diversity were mainly associated with replacement of taxa. Variation partitioning results revealed that all beta diversity measures based on incidence data were governed primarily by local and land-use variables, whereas spatial variables were more relevant in driving beta diversity weighted by abundance data. Overall, by comparing different facets and components of beta diversity weighted by incidence versus abundance data, we suggest that incidence-weighted data may be more sensitive in portraying the impacts of urbanization on macroinvertebrate diversity. This likely resulted from the fact that incidence-weighted data shows the importance of rare taxa in shaping homogenization induced by urbanization.

Niche expansion by indigenous fish species following the introduction of rainbow trout (Oncorhynchus mykiss) in a subtropical river system, upper Blyde River, South Africa

The introduction of alien predatory fish such as rainbow trout (Oncorhynchus mykiss) can have a significant effect on indigenous fish communities such as altering the structure and dynamics of food webs. Quantifying the trophic niche utilised by the alien fish species is therefore important to aid our understanding of how their feeding strategies might aid establishment, define their functional role and inform on potential impacts. This study assessed food web interactions between fish communities in river reaches that are invaded by O. mykiss and sections that are free of invasions in the upper Blyde River catchment, South Africa. It specifically evaluated the hypothesis that O. mykiss invasion is likely to lead to a decrease in the trophic functional diversity through predation and that indigenous fish will shift their trophic niche to either minimise competition with, or avoid predation, by O. mykiss. Gut content and stable isotope analyses were used to determine trophic interactions. Fish communities in uninvaded areas utilised fewer and similar food sources and occupied lower trophic levels, than fish communities in invaded areas. Oncorhynchus mykiss fed mainly on invertebrates and at trophic levels similar to and or lower than indigenous fishes. This suggests that their current impact on indigenous fish communities is mainly through competition for resources. We posit that O. mykiss invasions reduced the abundance of indigenous fishes and thereby reduced predation pressure on aquatic invertebrates, with a knock-on effect on the trophic interrelationships among fish assemblages. Our findings are consistent with other studies in South Africa that have shown that the impacts of O. mykiss invasion can lead to a decline and fragmentation of indigenous species populations and niche shifts.

The combined effects of land use and seasonal environmental factors on stream food web structure

Land use and seasonal changes in environmental conditions influence biological communities and their trophic interactions in riverine ecosystems. However, how land use and the seasonality of environmental conditions jointly influence the food web structure of riverine ecosystems remains unclear. Here, we conducted a comparative study on basal resources, macroinvertebrates, and fish at woodland and urban sites to explore the combined effects of land use and environmental conditions during spring, autumn, and winter on the food web structure of a subtropical river in China. We used δ13C and δ15N to trace consumers’ diets and calculate community-level metrics within food webs. At woodland sites, we found that allochthonous sources contributed significantly more to consumers’ diets in the high flow season (53%) than in the low flow season (around 30%), but allochthonous sources contributed less than 30% at urban sites. The seasonal flooding facilitated the acquisition of terrestrial sources at woodland sites, while the impervious surface cover at urban sites cut off the influx of terrestrial inputs. The isotopic niche space of basal resources at both woodland and urban sites was significantly higher in the high flow season. However, the isotopic niche space of consumers at urban sites was not significantly different between seasons. Trophic length, niche width, and trophic redundancy showed no seasonal differences at urban sites, but trophic length increased significantly during the high flow season at woodland sites. Our temporal food web study at urban and woodland rivers illustrated the combined effects of land use and seasonal environmental conditions on the food web structure, and highlighted the role of allochthonous carbon in supporting biological communities and the importance of lateral and longitudinal connectivity in river ecosystems.

Ecological drivers of macroinvertebrate metacommunity assembly in a subtropical river basin in the Yangtze River Delta, China.

Identifying the underlying ecological drivers of macroinvertebrate community assembly is fundamental to metacommunity ecology. Comparably, determining the influence of different drivers on beta diversity patterns can provide insight into processes governing community organization. Exploring the ecological drivers of metacommunity and beta diversity are major avenues to improve bioassessment, restoration, and river management, which are still poorly explored in China, especially in subtropical highly developed river networks. To address this gap, we use a dataset (macroinvertebrate communities and environmental variables) collected from the Yangtze River Delta, China to test the above ideas. We used the K-means clustering method to divide 405 river sites into three anthropogenic impacted groups, nearly pristine sites, moderately impacted sites, and heavily impacted sites, and subsequently used partial Mantel tests to investigate how species sorting and dispersal shaped the metacommunity that varied with the levels of anthropogenic impacts and to explore the responses of different components of beta diversity to environmental and spatial distances among sites for each group. Our results revealed that both species sorting and dispersal shape communities, but the importance of species sorting and dispersal varied with the levels of anthropogenic impacts. Nearly pristine sites were mostly shaped only by species sorting, while heavily impacted sites were shaped by dispersal. We also found that turnover was by far the dominant component of beta diversity across all levels of impact. Therefore, we encourage that environmental variables and spatial processes should be considered in bioassessment approaches. In addition, it is essential to focus on maintaining habitat heterogeneity and identifying and protecting regional species pools that could improve local biodiversity through dispersal for ecosystem management of the Yangtze River Delta of China.

Branched glycerol dialkyl glycerol tetraethers as indicators for environmental parameters in a subtropical mountainous river, southern China

Indices based on branched glycerol dialkyl glycerol tetraethers (brGDGTs) have been useful in paleo-environmental reconstructions, although their applicability in river-estuarine sediments remains unclear. This study aims to investigate the correlation between brGDGT-based indices and modern environmental parameters, and to discuss possible impact factors. In this study, we discussed the applicability of several brGDGT-based indices, with specific interests on the IRx’ (the relative abundance of 6- vs. 5-methylated brGDGTs) and CBT’ (cyclization index of branched tetraethers based on the separation of 5- and 6-methylated brGDGTs) for pH reconstruction, and MBT’5ME (the methylation index of branched tetraethers based on the separation of 5- and 6-methylated brGDGTs) for MAAT (mean annual air temperature) reconstruction. From the Jiulong River (JLR) catchment, we have analyzed both riverbank soil samples and riverbed surface sediment samples for brGDGTs, with the following main findings: Firstly, for pH reconstruction, the indices CBT’ and IRx’ are useful in soil and fluvial sedimentary settings. Secondly, for MAAT reconstruction, the MBT’5ME index is sensitive to the air temperature, and the BayMBT0 model performs relatively better than other global or regional calibrations in the study area; By employing the BayMBT0 model, MAAT reconstructed from soils are higher than the measured MAAT, but are generally lower than the measured MAAT when reconstructed from riverbed surface sediments. Thirdly, the disagreement between the reconstructed results from the two sedimentary archives suggests that: Signatures of brGDGTs in the aquatic environment are under strong impacts of in situ produced brGDGTs; Impacts of upper-reach brGDGTs input might have resulted in the increasingly negative offset for the reconstructed MAAT downstream; And influences of complex aquatic biogeochemical conditions on sedimentary brGDGT signals are site-dependent in this area. Finally, brGDGT-based indices are sensitive to the source of brGDGTs, e.g., soil vs aquatic in situ brGDGTs; And terrigenous brGDGTs have been exposed to a range of complex biogeochemical processes before their deposition in the estuary. Based on these findings, we suggest that at least the above impact factors need to be taken into consideration when interpreting the brGDGT signals from estuarine archives, and thus, multi-proxy studies are encouraged. The findings of this study provide a better understanding of the correlation between brGDGT-based indices and modern environmental parameters in subtropical river systems, and provide useful information for interpreting the paleo-environmental signals based on brGDGTs from fluvial-estuarine archives.

Environmental controls of plankton community dynamics in a sub-tropical river system of Bangladesh

Environmental controls of plankton community dynamics in a sub-tropical river system of Bangladesh

Dam Construction Impacts Fish Biodiversity in a Subtropical River Network, China

Dams and diversions are a primary threat to freshwater fish biodiversity, including the loss of species and restructuring of communities, often resulting in taxonomic homogenization (increased similarity) over time. Mitigating these impacts requires a strong scientific understanding of both patterns and drivers of fish diversity. Here, we test whether different components of fish biodiversity have changed in response to major dam construction, and whether these patterns are predictable as a function of key environmental factors in the Gan River Basin, China. The results showed that total and native species alpha diversity have declined from the historical period (pre-dam) to the current period (post-dam). A total of 29 native species are lost, while 6 alien species were gained over time. We found evidence for fish faunal homogenization in the Gan River Basin, with a slight (1%) increase in taxonomic similarity among river basins from the historical period to the current period. Additionally, we revealed significant associations between drainage length, drainage area, and average air temperature, and alpha and beta fish diversity. This study provides new insight into the patterns and drivers of fish biodiversity change in the broader Yangtze River Basin and helps inform management efforts seeking to slow, and even reverse, current trajectories of biodiversity change.

Impact of the long-term precipitation and land use changes on runoff variations in a humid subtropical river basin of China

Study regionLiuxihe River Basin in Guangdong province, China. Study focusDeciphering the runoff variation and its causes is crucial for catchment water management. The impacts of long-term precipitation aand land use change on the annual surface runoff were quantitatively analysed using various methods for the period of 1948–2019. New hydrological insightsWe found that abrupt change occurred in 2009 for precipitation and 2003 for runoff, and the slope of precipitation-runoff cumulative curve changed after 2000 with an increase of ~122 %. This indicated that human activities in the early 2000 s may have resulted in a marked increase of runoff in addition to precipitation. The dominant land use transfer was from croplands to constructed lands, most profoundly during 2000–2010. The multiple linear regression analysis indicated that the increase of precipitation and the cropland-to-constructed land transitions were nearly equally responsible for the runoff increase. In total, the long-term precipitation and major land use change explained ~79 % of annual runoff variation. Furthermore, the effect of surface water area especially the pond area was discussed, and the negative linear relationship was found not statistically significant, but the impact of pond area ratio and its storage capacity on peak flow during rainstorms is worth further exploration towards flood mitigation, which can serve as an important and effective measure for nature-based management.

Bacterioplankton community indicators for seasonal variation in a fragmented subtropical river.

In this study, spatiotemporal investigations were conducted along five cascade dams in the main channel of the North River of China during 2019 to explore bacterioplankton community indicators of water environments in a fragmented, highly regulated river. Bacterioplankton communities were good bioindicators of temporal variation in river environments, especially when considering the bacterial class level. Specifically, the most dominant bacterial classes (Gammaproteobacteria, Oxyphotobacteria, and Actinobacteria) and sub-dominant bacterial classes (Bacteroidia, Betaproteobacteria, and Acidimicrobiia) exhibited obvious temporal variation. Rainfall, water temperature (WT), water transparency (SD), and pH were all highly associated with temporal variation. In contrast, bacterioplankton indicators of spatial variation were limited to individual dominant bacterial classes for individual study periods, while rainfall, total phosphorus (TP), and pH were also associated with spatial variation. Clustering of bacterioplankton community compositions revealed that temporal differences were much stronger than spatial differences, which is consistent with most environmental parameters exhibiting obvious temporal differences, but minimal spatial differences. A possible reason for these observations could be that river fragmentation caused by cascade dams weakened spatial differences in communities, with WT, rainfall, and river runoff playing key roles in these patterns. In conclusion, bacterioplankton communities were good bioindicators of water environments in the fragmented river ecosystem of this study and their temporal variation was more apparent than their spatial variation.

Review: Phytochemical composition, medicinal uses and other utilization of Nypa fruticans

Abstract. Nugroho GD, Wiraatmaja MF, Pramadaningtyas PS, Febriyanti S, Liza N, Naim DM, Ulumuddin YI, Setyawan AD. 2020. Review: Phytochemical composition, medicinal uses and other utilization of Nypa fruticans. Bonorowo Wetlands 10: 51-65. Nypa fruticans Wurmb or “nipa/nipah” is a palm plant in mangrove ecosystems along tropical and subtropical river estuaries affected by tides. Nipa has a high abundance in the mangrove forest ecosystem. Therefore, knowledge and utilization of this nipa plant need to be sought and examined more deeply to optimize its utilization. This review aims to find out the phytochemical composition, medicinal uses, and other utilization of nipa plants. The main phytochemical composition of nipa is polyphenols, phenolics, alkaloids, tannins, flavonoids, and saponins. However, other chemical compounds such as ethyl acetate, chloroform, hexane, triterpenoids, phenol hydroquinone, diterpenes, and steroids are still available. The many chemical compounds in nipa can be used as raw materials in a product, especially in modern medicines. Based on people's beliefs in several tropical countries that nipa has the potential of herbal medicine to treat fever, gout, kidney stones, energy booster, aid digestion, as a cure for certain chronic diseases and metabolic syndromes such as diabetes and hypertension, treat asthma, leprosy, tuberculosis, sick throat, liver disease, snakebite, as a pain reliever, and also be used as a sedative and able to expel excess wind in the body. Then in the pharmacological aspect, nipa has antioxidant, antidiabetic, antimicrobial (antifungal and antibacterial), anticancer, anti-inflammatory, antinociceptive, antihyperglycemic and analgesic activities. In addition to other benefits outside of drugs, nipa is used as a roof for houses, cattle pens, huts in the garden, broomsticks, handicrafts, fishing gear, food and drink sources, and sources of renewable fuel.

Impact of extreme climatic events on unionid mussels in a subtropical river basin

Impact of extreme climatic events on unionid mussels in a subtropical river basin

Differential Response of Nutrients to Seasonal Hydrological Changes and a Rain Event in a Subtropical Watershed, Southeast China

A large amount of terrestrial nutrients are discharged into the ocean through rivers. However, the impact of seasonal hydrological variations on riverine nutrient concentrations and fluxes remains unclear, especially in the medium-sized subtropical rivers that are highly influenced by human activities. In this study, we investigated the monthly changes in nutrient concentrations (soluble reactive phosphorus, SRP; dissolved silicate, DSi; and dissolved inorganic nitrogen, DIN) in the North Stream (NS) and West Stream (WS) of the Jiulong River (JLR). The results show that the concentrations of SRP and DSi in the NS and the WS displayed a similar seasonal variability, which was different from the pattern of DIN. Hydrological conditions, chemical fertilizer loss and biogeochemical processes are responsible for the seasonal changes in the nutrients in the two streams, especially during extreme rain events. Nutrient concentrations in the NS exhibited a clockwise trajectory along with river discharge during rain events, while a reverse pattern in the WS was observed since it experienced a moderately long rain event. Different rainfall features between the two main tributaries resulted in the majority of nutrients being exported at the start of the rain event in the NS and the end of rain event in the WS. Indeed, the annual high flow (Q/Qm > 3) accounts for ~17.3% of the annual nutrient flux in the JLR even though this period spans only ~4.0% of a year, which suggests the importance of rain events on nutrient export in these subtropical rivers. Although the annual fluxes of DIN and SRP in the JLR were smaller than many rivers worldwide, higher areal yields of DIN and SRP were observed, indicating that the JLR is highly influenced by human activities. Our study systematically evaluated the response of nutrient concentrations to hydrological changes in two tributaries of the JLR, which is useful in better understanding the nutrient dynamics in medium-sized subtropical rivers.

Spawning Season, Fecundity, and Size at First Maturity of a Freshwater Mullet, Minimugil cascasia (Hamilton 1822), from subtropical River Ganga, India

Spawning Season, Fecundity, and Size at First Maturity of a Freshwater Mullet, Minimugil cascasia (Hamilton 1822), from subtropical River Ganga, India

Dynamic of riverine pCO2, biogeochemical characteristics, and carbon sources inferred from δ13C in a subtropical river system

Rivers significantly contribute to the global carbon budget, but data limitations and uncertainty are hampered by CO2 quantification in the global rivers. Thus, this study estimated riverine pCO2 by employing the pH-alkalinity-temperature method, and dissolved inorganic (DIC), dissolved organic (DOC), particulate organic (POC) carbon, and their isotopes (δ13C) with Chlorophyll-a (Chl a) were measured in river water samples from 26 sampling sites for characterization and source identification in the Yangtze River system. The estimated pCO2 varies from (120 ppm) to (3400 ppm) with an average (1085 ppm) across the Yangtze River and pCO2 is almost three times oversaturated than the ambient air (380 ppm). The downstream sites pronounced elevated pCO2 than the upstream sites. The relationship of δ13CDIC and pCO2 indicated that pCO2 control is seasonally independent. The significant correlations between DOC, POC, and pCO2 revealed that organic carbon influenced pCO2 in the river. The seasonal fluctuations of pCO2 were observed with an average of (762.23 ppm) and (1407.35 ppm) in winter and summer, respectively. δ13CDIC showed that the metabolic process has a negligible influence on DIC, δ13CDIC, and pCO2. δ13CDIC values increased from −8.95‰ to −4.91‰ during summer, whereas winter increased from −19.76‰ to −1.97‰ suggesting that DIC derived from carbonate weathering, dissolution of atmospheric and soil CO2. The δ13CDOC (−30.43‰ to −24.05‰) and δ13CPOC (−29.87‰ to −23.37‰) values confirmed that organic carbon mainly derived from the degradation of organic materials in soil. δ13CDIC revealed that anthropogenic sewage discharge slightly modified DIC composition. Overall, this study provides new insight into recent seasonal fluctuations of the pCO2, DOC, POC, DIC, δ13C, and their inputs. Thus, these variations and inputs of carbon transported by the Yangtze River could have a significant influence not only on the biogeochemical cycle and ecosystem process but also on the global carbon budget.

Unravelling the spatiotemporal variation of pCO2 in low order streams: Linkages to land use and stream order

Headwater streams make the majority of cumulative stream length in a river basin, carbon dioxide (CO2) emission from headwater (low order) streams is thus an essential component. Anthropogenic activities in headwater areas such as land use change and land use practices can strongly modify terrestrial carbon and nutrient input, which could affect the level of partial pressure of dissolved carbon dioxide (pCO2) and CO2 degassing from streams. However, there are large uncertainties in estimates due to the lack of data in subtropical rivers of rapidly developing rural regions. The spatiotemporal variation and driving factors of the pCO2 and CO2 degassing from low-order streams remain to be explored. In this study, we assess multi-spatial scale effects of land use on pCO2 dynamics in seven headwater tributary rivers in Central China during 2016, 2017 and 2018 in rainy and dry seasons. Our results reveal that the stream pCO2 level consistently increases as the stream order increases from 1 to 3 under apparent seasonal variations. Riverine pCO2 is positively related to the percentage of urban land and cropland surrounding the river segments, but is negatively related to the percentage of forest land. The stream pCO2 is more closely correlated with the 1000 and 2000 m diameters of circular buffers at upstream sampling sites than the circular buffers with 100 and 500 m diameters. There exist significant relationships of pCO2 with the concentrations of TN, TP, DO, and DOC in the low-order streams. The partial redundancy analysis quantifies the relative importance of anthropogenic land uses, natural factors and water chemical variables in mediating stream pCO2, showing that influences of anthropogenic land uses (urban and cropland) on pCO2 decrease, with a percentage role of 34%, 14%, and 4% in the 1st-, 2nd- and 3rd-order streams, respectively. The impact of nutrients on pCO2, however, increases as the stream order increases. Urban influence on stream pCO2 also decreases as stream order increases. Our study highlights the effect of land use/land cover types and stream order on riverine pCO2 and provides new insight into estimating CO2 emission in headwater streams. Future studies are needed on the linkage between riverine CO2 degassing and stream orders under changing land use conditions.

Effects of Covid-19 Era on a Subtropical River Basin in Bangladesh: Perspectives of Eco-Restoration Strategies and Probable Human Health Risks

Effects of Covid-19 Era on a Subtropical River Basin in Bangladesh: Perspectives of Eco-Restoration Strategies and Probable Human Health Risks

Ecological Drivers of Macroinvertebrate Metacommunity Assembly in a Subtropical River Basin in the Yangtze River Delta, China

Ecological Drivers of Macroinvertebrate Metacommunity Assembly in a Subtropical River Basin in the Yangtze River Delta, China

Water Quality Analysis in a Subtropical River with an Adapted Biomonitoring Working Party (BMWP) Index

Subtropical rivers in developing countries often lack adequate monitoring, which makes it difficult to comprehensively determine their water quality when faced with different anthropic impacts. There are no proper protocols in the regulations to incorporate indicators and adapt them to different biogeographic regions, limiting the potential success of conservation and restoration of river ecosystems. This study proposes implementing macroinvertebrates as bioindicators of water quality in river ecosystems, and modifying the calibration of the widely used Biomonitoring Working Party (BMWP) index for its adaptation in a subtropical river. The Duero River, Mexico, was used as an example in this study. Data were explored with multivariate statistics, and the water quality and habitat values were averaged to obtain the families’ bioindication values and the index categories. The BMWP adequately described a deterioration gradient from the origin to the river mouth (from fair to extremely polluted), with some intermediate recovery points related to the presence of springs. Its performance was compared with other biological indices and exhibited a positive relationship with all of them. In addition, how BMWP changed over time was analyzed by examining previous samples, and highlighted increased river deterioration over time. A calibrated BMWP will allow for long-term monitoring at a low cost.

Anthropogenic activities and seasonal properties jointly drive the assemblage of bacterial communities in subtropical river basins

Anthropogenic activities have inevitably impacted riverine ecosystems, yet their overall contribution to the assemblage of bacterial communities at a large river basin scale remains unclear. In this study, 16S amplicon sequencing was implemented to investigate the bacterial ecosystems in paired water and sediment of North River and West River basins in South China., which contains various anthropogenic environments (e.g., rural/urban area, mining area and livestock area). Subsequently, the links between bacterial community and various types of emerging pollutants in river water were analyzed. The results show that the bacterial assemblage of water and sediment had their own properties that the bacterial community of sediment were mainly affected by seasonal properties, while the bacterial community of water were affected by both seasons and anthropogenic activities. Therein, the aquatic bacterial compositions and abundances were driven by changes in temperature, dissolved oxygen and the emerging pollutants. The dominant phyla Proteobacteria and Firmicutes exhibited adaptability to the mining-affected regions, therein many clades (e.g., Beijerinckiaceae, Acetobacteraceae and Mycobacteriaceae) were also prevalent in the livestock-affected and densely-populated regions. In addition, these two phyla presented associations to the antibiotic resistance in water. The levels of antibiotics, relative antibiotic resistance gens (ARGs) and non-antibiotic pharmaceuticals (NAPs) were closely related to bacterial community composition, diversity and functional diversity, indicating their drive in shifting bacterial communities. Collectively, this work provides a basis for understanding the contribution of anthropogenic activities in shifting bacterial community at a large river basin scale. Further, the results provide new insights for expansion of ecological assessment.