Subtropical Ocean Research Articles

Eukaryotic plankton community assembly and influencing factors between continental shelf and slope sites in the northern South China Sea

Eukaryotic plankton are pivotal members of marine ecosystems playing crucial roles in marine food webs and biogeochemical cycles. However, understanding the patterns and drivers of their community assembly remains a grand challenge. A study was conducted in the northern South China Sea (SCS) to address this issue. Here, 49 samples were collected and size-fractionated from discrete depths at continental shelf and continental slope in the northern SCS over a diel cycle. From high throughput sequencing of the 18S rDNA gene V4 region, 2463 operational taxonomic units (OTUs) were retrieved. Alveolata and Opisthokonta overwhelmingly dominated the assemblages in the abundance (44.76%, 31.08%) and species richness (59%, 12%). Biodiversity was higher in the slope than the shelf and increased with depth. Temperature and salinity appeared to be the most important deterministic drivers of taxon composition. Community structure was influenced by multiple factors in the importance order of: environmental factors (temperature + salinity) > spatial factor > water depth > sampling time. Furthermore, the neutral model explained more variations in the smaller-sized (0.22–3 μm) community (24%) than larger-sized (3–200 μm) community (16%) but generally explained less variations than did deterministic processes. Additionally, our data indicated that the larger plankton might be more environmentally filtered and less plastic whereas the smaller plankton had stronger dispersal ability. This study sheds light on the differential contributions of the deterministic process and stochastic process and complexities of assembly mechanisms in shaping the community assembly of micro-nano and pico-eukaryotic biospheres in a subtropical ocean.

A nutrient relay sustains subtropical ocean productivity

The expansive gyres of the subtropical ocean account for a significant fraction of global organic carbon export from the upper ocean. In the gyre interior, vertical mixing and the heaving of nutrient-rich waters into the euphotic layer sustain local productivity, in turn depleting the layers below. However, the nutrient pathways by which these subeuphotic layers are themselves replenished remain unclear. Using a global, eddy-permitting simulation of ocean physics and biogeochemistry, we quantify nutrient resupply mechanisms along and across density surfaces, including the contribution of eddy-scale motions that are challenging to observe. We find that mesoscale eddies (10 to 100 km) flux nutrients from the shallow flanks of the gyre into the recirculating interior, through time-varying motions along density surfaces. The subeuphotic layers are ultimately replenished in approximately equal contributions by this mesoscale eddy transport and the remineralization of sinking particles. The mesoscale eddy resupply is most important in the lower thermocline for the whole subtropical region but is dominant at all depths within the gyre interior. Subtropical gyre productivity may therefore be sustained by a nutrient relay, where the lateral transport resupplies nutrients to the thermocline and allows vertical exchanges to maintain surface biological production and carbon export.

Direct Measurement of CO2 Air‐Sea Exchange Over a Desert Fringing Coral Reef, Gulf of Eilat (Aqaba), Israel

AbstractUnderstanding the role of coral reefs on air‐sea CO2 exchange is essential to accurately estimate carbon budgets throughout the tropical and subtropical oceans. Results from research conducted in humid marine environments show coral reefs are mainly net sources of CO2 to the atmosphere due to calcification being dominant over respiration and dissolution. However, coral reefs are also found bordering deserts in the subtropical high‐pressure belt. Here, we present results from concurrent direct measurements of air‐sea CO2 exchange over fringing coral reefs and open sea bordered by hyper‐arid deserts in the Gulf of Eilat (Aqaba). The fringing coral reefs were found to be a net sink of CO2 at −1.62 ± 4 μmol m−2 s−1 sequestering 1.96 times more CO2 than adjacent open sea. The CO2 fluxes showed a negative correlation with some local meteorological parameters such as humidity, wind speed, and net radiation but were positively correlated to air temperature. The air‐sea CO2 fluxes were also found to be correlated with dust, which is believed to trigger metabolic processes in bacteria and phytoplankton in the low‐nutrient, low‐chlorophyll sea of the study site. Results are compared to measurements from the humid marine setting of the Great Barrier Reef, Australia.

Global satellite water classification data products over oceanic, coastal, and inland waters

Satellites have generated extensive data of remote sensing reflectance spectra (Rrs(λ)) covering diverse water classes or types across global waters. Spectral classification of satellite Rrs(λ) data allows for the distinguishing and grouping of waters with characteristic bio-optical/biogeochemical features that may influence the productivity of a given water body. This study reports new satellite water class products (Level-2 and Level-3) from the Visible Infrared Imaging Radiometer Suite (VIIRS). We developed and implemented a hyperspectral scheme that accounts for the Rrs(λ) spectral shapes and globally resolves oceanic, coastal, and inland waters into 23 water classes. We characterized the light absorption and scattering coefficients, chlorophyll-a concentration, diffuse attenuation coefficient, and suspended particulate matter for individual water classes. It is shown that the water classes are separable by their distinct bio-optical and biogeochemical properties. Furthermore, validation result suggests that the VIIRS water class products are accurate globally. Finally, we examined the spatial and temporal variability of the water classes in case studies for a demonstration of applications. The water class data in open oceans reveal that the subtropical ocean gyres have experienced dramatic expansion over the last decade. In addition, the water class data appear to be a valuable (and qualitative) indicator for water quality in coastal and inland waters with compelling evidence. We stress that this new satellite product is an excellent addition to the aquatic science database, despite the need for continuous improvement toward perfection.

Observing system simulation experiments reveal that subsurface temperature observations improve estimates of circulation and heat content in a dynamic western boundary current

Abstract. Western boundary currents (WBCs) form the narrow, fast-flowing poleward return flows of the great subtropical ocean gyres and are sources of rapidly varying mesoscale eddies. Accurate simulation of the vertical structure, separation latitude, and ocean heat content of WBCs is important for understanding the poleward transport of heat in the global ocean. However, state estimation and forecasting in WBC regions, such as the East Australian Current (EAC), the WBC of the South Pacific subtropical gyre, is challenging due to their dynamic nature and lack of observations at depth. Here we use observing system simulation experiments to show that subsurface temperature observations in a high eddy kinetic energy region yield large improvement in representation of key EAC circulation features, both downstream and ∼ 600 km upstream of the observing location. These subsurface temperature observations (in concert with sea surface temperature and height measurements) are also critical for correctly representing ocean heat content along the length of the EAC. Furthermore, we find that a more poleward separation latitude leads to an EAC and eddy field that is represented with far reduced error, compared to when the EAC separates closer to the Equator. Our results demonstrate the importance of subsurface observations for accurate state estimation of the EAC and ocean heat content that can lead to marine heatwaves. These results provide useful suggestions for observing system design under different oceanographic regimes, for example, adaptive sampling to target high energy states with more observations and low energy states with fewer observations.

Global beta diversity patterns of microbial communities in the surface and deep ocean

AbstractAimDispersal and environmental gradients shape marine microbial communities, yet the relative importance of these factors across taxa with distinct sizes and dispersal capacity in different ocean layers is unknown. Here, we report a comparative analysis of surface and deep ocean microbial beta diversity and examine how these patterns are tied to oceanic distance and environmental gradients.LocationTropical and subtropical oceans (30°N–40°S).Time period2010–2011.Major taxa studiedProkaryotes and picoeukaryotes (eukaryotes between 0.2 and 3 μm).MethodsBeta diversity was calculated from metabarcoding data on prokaryotic and picoeukaryotic microbes collected during the Malaspina expedition across the tropical and subtropical oceans. Mantel correlations were used to determine the relative contribution of environment and oceanic distance driving community beta diversity.ResultsMean community similarity across all sites for prokaryotes was 38.9% in the surface and 51.4% in the deep ocean, compared to mean similarity of 25.8 and 12.1% in the surface and deep ocean, respectively, for picoeukaryotes. Higher dispersal rates and smaller body sizes of prokaryotes relative to picoeukaryotes likely contributed to the significantly higher community similarity for prokaryotes compared with picoeukaryotes. The ecological mechanisms determining the biogeography of microbes varied across depth. In the surface ocean, the environmental differences in space were a more important factor driving microbial distribution compared with the oceanic distance, defined as the shortest path between two sites avoiding land. In the deep ocean, picoeukaryote communities were slightly more structured by the oceanic distance, while prokaryotes were shaped by the combined action of oceanic distance and environmental filtering.Main conclusionsHorizontal gradients in microbial community assembly differed across ocean depths, as did mechanisms shaping them. In the deep ocean, the oceanic distance and environment played significant roles driving microbial spatial distribution, while in the surface the influence of the environment was stronger than oceanic distance.

Trichodesmium erythraeum produces a higher photocurrent than other cyanobacterial species in bio-photo electrochemical cells

The increase in world energy consumption, and the worries from potential future disasters that may derive from climate change have stimulated the development of renewable energy technologies. One promising method is the utilization of whole photosynthetic cyanobacterial cells to produce photocurrent in a bio-photo electrochemical cell (BPEC). The photocurrent can be derived from either the respiratory or photosynthetic pathways, via the redox couple NADP+/NADPH mediating cyclic electron transport between photosystem I inside the cells, and the anode. In the past, most studies have utilized the fresh-water cyanobacterium Synechocystis sp. PCC 6803 (Syn). Here, we show that the globally important marine cyanobacterium Trichodesmium erythraeum flourishing in the subtropical oceans can provide improved currents as compared to Syn. We applied 2D-fluorescence measurements to detect the secretion of NADPH and show that the resulting photocurrent production is enhanced by increasing the electrolyte salinity, Further enhancement of the photocurrent can be obtained by the addition of electron mediators such as NAD+, NADP+, cytochrome C, vitamin B1, or potassium ferricyanide. Finally, we produce photocurrent from additional cyanobacterial species: Synechocystis sp. PCC6803, Synechococcus elongatus PCC7942, Acaryochloris marina MBIC 11017, and Spirulina, using their cultivation media as electrolytes for the BPEC.

Crocosphaera watsonii.

Crocosphaera watsonii is a marine unicellular cyanobacterium that fixes carbon during the day and nitrogen during the night. They are abundant in tropical and subtropical oceans, providing both bioavailable carbon and nitrogen to the ecosystem, altering local and possibly global biogeochemical cycling. The temporal segregation of nitrogen fixation from oxygenic photosynthesis helps to protect nitrogenase, the oxygen-sensitive enzyme responsible for nitrogen fixation. The diel rhythm of carbon and nitrogen fixations fluctuates the cellular carbon to nitrogen ratio.

The Spatial Variation in Chlorophyte Community Composition From Coastal to Offshore Waters in a Subtropical Continental Shelf System

Globally, the continental shelf occupies less than 10% of the total sea surface, but supports substantial primary production and fisheries. Photosynthetic picoeukaryotes (PPE) are important primary producers in marine ecosystems, and chlorophytes make a significant contribution to PPE abundance. Although the distribution of chlorophytes has been widely studied, little is known about how their community composition varies along the coastal-offshore gradient in subtropical continental shelf waters. To better understand their spatial variations, we employed metabarcoding data of 18S rRNA V4 gene to examine chlorophyte composition within the surface and deep chlorophyll maximum (DCM) layers over the continental shelf in the East China Sea (ECS) with high-resolution sampling. Our results indicate a higher chlorophyte diversity in the surface layer than in the DCM. In addition, we found that chlorophytes in oligotrophic Kuroshio surface water were the most diverse, with a higher percentage of Chloropicophyceae, prasinophyte clade V, prasinophyte clade IX, Palmophyllophyceae and Chlorodendrophyceae. In more eutrophic waters along the coast and within the DCM layer, chlorophytes were dominated by Mamiellophyceae. A significant spatial variation in community composition occurred along the nutrient gradient in the subtropical continental shelf ecosystem, varying from Mamiellophyceae-dominant in coastal water to Chloropicophyceae-dominant in offshore water. Furthermore, at a low saline coastal station, which had the lowest chlorophyte diversity, Picochlorum (Trebouxiophyceae) was dominant. Overall, chlorophyte diversity was positively correlated with temperature, and negatively correlated with silicate concentration. This study revealed that terrestrial input into continental systems influences chlorophyte diversity in the subtropical northwestern Pacific Ocean.

How can the positive Indian Ocean Dipole events co‐occur with La Niña?

AbstractPositive/negative Indian Ocean Dipole (IOD) tends to co‐occur with El Niño/La Niña. Five positive IODs, however, are observed under La Niña condition in 1961, 1966, 1967, 2007, and 2011. This study uses the monthly ocean temperature and atmospheric reanalysis datasets from ECMWF to investigate how these positive IOD events occurred. It is found that the five positive IODs are consistently accompanied by a moderately strong positive subsurface IOD (Sub‐IOD) event that initiated earlier. The Sub‐IOD‐related oceanic dynamical processes including oceanic Kelvin and Rossby waves help to promote the development of the positive IODs. More specifically, the equatorial easterly anomalies in the Tropical Indian Ocean (TIO) that are crucial for the development of the positive Sub‐IODs and IODs in 1961/2011 are triggered by an anticyclonic curl over South Indian Ocean that is induced by a Southern Hemispheric Indian Ocean Subtropical Dipole mode. The crucial equatorial easterly anomalies in 1967 are triggered by a thermocline warming in the southwestern TIO induced by westward downwelling oceanic Rossby waves, while in 1966/2007, the equatorial easterly anomalies are connected with the anomalous Walker circulation resulting from the certain sea surface temperature anomaly pattern over the equatorial Pacific. On the other hand, the remote effects of La Niña on TIO are relatively weaker, being overwhelmed by the above triggering processes.

Spatial patterns and environmental associations of deep scattering layers in the northwestern subtropical Pacific Ocean

Spatial patterns and environmental associations of deep scattering layers in the northwestern subtropical Pacific Ocean

Quasi-Biennial Variability of Indian Ocean Subtropical Mode Water Subduction Driven by Atmospheric Circulation Modes during the Argo Period

Abstract Argo data were used to investigate interannual variability in the subduction rate of the Indian Ocean Subtropical Mode Water (IOSTMW). The IOSTMW subduction rate in the southwestern part of the southern Indian Ocean subtropical circulation varies substantially from year to year without significant trend during the Argo period 2005–19. The variability of the IOSTMW subduction rate is quasi-biennial and dominated by the lateral induction term associated with the wintertime mixed layer depth (MLD). The contribution of vertical pumping to the change of subduction rate is relatively small. This variability in the subduction rate directly contributes to IOSTMW volume with a quasi-biennial variation. The quasi-biennial variations in the IOSTMW subduction and volume reflect the variability of the overlying atmosphere. The wintertime Mascarene high (MH) modulates the winter MLD in the subduction area through changes in heat fluxes and wind forcing. The quasi-biennial variability in MH is associated with the southern annular mode, but it was disturbed during two El Niño events in 2009 and 2015/16. Variability of the wintertime MH plays a key role in driving the quasi-biennial variability of the IOSTMW subduction during the Argo period.

Nickel superoxide dismutase protects nitrogen fixation in Trichodesmium

AbstractTrichodesmium, a major diazotroph in tropical and subtropical oceans, provides a significant amount of bioavailable nitrogen in oligotrophic oceans. Remarkably, the cyanobacteria carry out nitrogen fixation and photosynthesis simultaneously under high light (HL) conditions. Here, we hypothesize that nickel superoxide dismutase (NiSOD) protects the nitrogen fixation process from oxidative stress generated in photosynthesis under high solar radiation conditions. We found that the variations in nitrogen fixation rates closely corresponded to Ni availability under HL conditions. The expression of NiSOD and two representative photosynthetic proteins also corresponded to variations in both nitrogen fixation rates and Ni availability under HL conditions. Trichodesmium collected in the field had a high Ni quota and elevated SOD activity. The evidence supports the conclusion that NiSOD protects nitrogen fixation in Trichodesmium from oxidative stress. The ability to utilize NiSOD may be a major advantage for Trichodesmium under high solar radiation conditions.

Uncertainty in Projected Changes in Precipitation Minus Evaporation: Dominant Role of Dynamic Circulation Changes and Weak Role for Thermodynamic Changes

AbstractEnd of century projections from Coupled Model Intercomparison Project (CMIP) models show a decrease in precipitation over subtropical oceans that often extends into surrounding land areas, but with substantial intermodel spread. Changes in precipitation are controlled by both thermodynamical and dynamical processes, though the importance of these processes for regional scales and for intermodel spread is not well understood. The contribution of dynamic and thermodynamic processes to the model spread in regional precipitation minus evaporation (P − E) is computed for 48 CMIP models. The intermodel spread is dominated essentially everywhere by the change of the dynamic term, including in most regions where thermodynamic changes drive the multi‐model mean response. The dominant role of dynamic changes is insensitive to zonal averaging which removes any influence of stationary wave changes, and is also evident in subtropical oceanic regions. Relatedly, intermodel spread in P − E is generally unrelated to climate sensitivity.

Distribution and Production of N2O in the Subtropical Western North Pacific Ocean During the Spring of 2020

Nitrous oxide (N2O) is an important greenhouse gas emitted in significant volumes by the Pacific Ocean. However, the relationship between N2O dynamics and environmental drivers in the subtropical western North Pacific Ocean (STWNPO) remains poorly understood. We investigated the distribution of N2O and its production as well as the related mechanisms at the surface (0–200 m), intermediate (200–1500 m), and deep (1500–5774 m) layers of the STWNPO, which were divided according to the distribution of water masses. We applied the transit time distribution (TTD) method to determine the ventilation times, and to estimate the N2O equilibrium concentration of water parcels last in contact with the atmosphere prior to being ventilated. In the surface layer, biologically derived N2O (ΔN2O) was positively correlated with the apparent oxygen utilization (AOU) (R2 = 0.48), suggesting that surface N2O may be produced by nitrification. In the intermediate layer, ΔN2O was positively correlated with AOU and NO3− (R2 = 0.92 and R2 = 0.91, respectively) and negatively correlated with nitrogen sinks (N*) (R2 = 0.60). Hence, the highest ΔN2O value in the oxygen minimum layer suggested N2O production through nitrification and potential denitrification (up to 51% and 25% of measured N2O, respectively). In contrast, the deep layer exhibited a positive correlation between ΔN2O and AOU (R2 = 0.92), suggesting that the N2O accumulation in this layer may be caused by nitrification. Our results demonstrate that the STWNPO serves as an apparent source of atmospheric N2O (mean air−sea flux 2.0 ± 0.3 μmol m-2 d-1), and that nitrification and potential denitrification may be the primary mechanisms of N2O production in the STWNPO. We predict that ongoing ocean warming, deoxygenation, acidification, and anthropogenic nitrogen deposition in the STWNPO may elevate N2O emissions in the future. Therefore, the results obtained here are important for elucidating the relationships between N2O dynamics and environmental changes in the STWNPO and the global ocean.

Manta and devil ray species occurrence and distribution in Venezuela, assessed through fishery landings and citizen science data.

Manta and devil rays (collectively mobulids) belong to the monogeneric Mobulidae, which currently comprises 10 species, including a putative third manta ray species (Mobula cf. birostris). These large planktivorous rays are distributed throughout the tropical and subtropical oceans of the world. To date, six mobulid species are reported for the western Atlantic Ocean and the Caribbean Sea, three of which had previously been reported in Venezuela (Mobula birostris, Mobula tarapacana and Mobula hypostoma). A preliminary assessment of fishery landings and citizen science data was conducted to further the scientific knowledge of mobulid species in Venezuela. Fisheries landing data were collected at Margarita Island between 2006 and 2007, and again in 2014. Data mining of internet search engines and social media platforms spanning the past two decades was also conducted. A total of 117 individuals of five mobulid species were recorded: Mobula sp. (n=27), M. birostris (n=36), M. tarapacana (n=3), Mobula mobular (n=26), Mobula thurstoni (n=14) and M. cf. birostris (n=11). The latter three species are the first confirmation of these species in Venezuela. The authors found no records of the previously reported M. hypostoma during this study. Although the occurrence of M. hypostoma in Venezuela remains possible because of the broad regional range of this species, its current presence in Venezuela is invalidated given the repeated misidentifications which have occurred in previous publications. The results of this study increase the number of reported mobulid ray species in Venezuela to five (excluding M. hypostoma). The overall data from juvenile manta rays and pregnant M. mobular and M. thurstoni recorded in this study, combined with the occurrence of all but one species of mobulid ray found in the western Atlantic Ocean, suggest Venezuela provides important habitat for this threatened family of rays.

The Biophysical Impacts of Deforestation on Precipitation: Results from the CMIP6 Model Intercomparison

AbstractDeforestation can impact precipitation through biophysical processes and such effects are commonly examined by models. However, previous studies mostly conduct deforestation experiments with a single model and the simulated precipitation responses to deforestation diverge across studies. In this study, 11 Earth system models are used to robustly examine the biophysical impacts of deforestation on precipitation, precipitation extremes, and the seasonal pattern of the rainy season through a comparison of a control simulation and an idealized global deforestation simulation with clearings of 20 million km2of forests. The multimodel mean suggests decreased precipitation, reduced frequency and intensity of heavy precipitation, and shortened duration of rainy seasons over deforested areas. The deforestation effects can even propagate to some regions that are remote from deforested areas (e.g., the tropical and subtropical oceans and the Arctic Ocean). Nevertheless, the 11 models do not fully agree on the precipitation changes almost everywhere. In general, the models exhibit higher consistency over the deforested areas and a few regions outside the deforested areas (e.g., the subtropical oceans) but lower consistency over other regions. Such intermodel spread mostly results from divergent responses of evapotranspiration and atmospheric moisture convergence to deforestation across the models. One of the models that has multiple simulation members also reveals considerable spread of the precipitation responses to deforestation across the members due to internal model variability. This study highlights the necessity of robustly examining precipitation responses to deforestation based on multiple models and each model with multiple simulation members.

Minimum Warming in the South Indian Ocean Thermocline in a Warming Climate Linked to Freshening Processes

Abstract The Southern Ocean (SO) is one of the key regions in absorbing and storing anthropogenic heat. An analysis of the CMIP6 models finds a distinct warming minimum/cooling and freshening in the subtropical ocean thermocline of the south Indian Ocean (SIO) under a medium-emission scenario (SSP245). The warming minimum/cooling has also been found in other warming scenarios in previous studies. However, the freshening here has received less attention. On account of increased precipitation in the models, the SO high latitudes get fresher in a warmer world. We show that this freshening anomaly is advected to the north of the deep mixed layer by the horizontal current and then subducts into the ocean interior in the SIO. As a result, the isopycnal surfaces become fresher, deeper, and cooler. This freshening and cooling signal then propagates to the north along isopycnals through the subtropical gyre and leads to freshening and cooling on the depth coordinates where the vertical movement of isopycnals (heaving) is insignificant. Lacking deep-enough mixed layers, the other two basins show smaller freshening and cooling signs in the models. Here the importance of freshening in temperature redistribution in the ocean interior in the SIO under extensive global warming is emphasized. The result helps interpret the future heat storage in the SO in a warmer world. Significance Statement The Southern Ocean (SO) is a key region in absorbing and storing anthropogenic heat. The observed past and simulated future warming in the SO show maximum values between 40° and 45°S and minimum values downward and northward of the deep mixed layers. CMIP6 models show the same pattern, and it is found that this pattern is most distinguished in the south Indian Ocean. The freshening anomaly advected to the deep mixed layers subducts into the ocean interior, leading to freshening and cooling signals on isopycnals. These signals spread with the subtropical gyre and induce warming minimum/cooling there. The result would help interpret the warming pattern in the SO in a warmer world.

Genome-wide survey and genetic characteristics of Ophichthus evermanni based on Illumina sequencing platform.

Ophichthidae fishes limit to continental shelf of all tropical and subtropical oceans and contain more than 350 species, representing the greatest specialization diversity in the order Anguiliformes. In the present study, we conducted a genome survey sequencing (GSS) analysis of Ophichthus evermanni by Illumina sequencing platform to briefly reveal its genomic characteristics and phylogenetic relationship. The first de novo assembled 1.97 Gb draft genome of O. evermanni was predicted based on K-mer analysis without obvious nucleotide bias. The heterozygosity ratio was 0.70%, and the sequence repeat ratio was calculated to be 43.30%. A total of 9016 putative coding genes were successfully predicted, in which 3587 unigenes were identified by gene ontology (GO) analysis and 4375 unigenes were classified into cluster of orthologous groups for enkaryotic complete genomes (KOG) functional categories. About 2,812,813 microsatellite motifs including mono-, di-, tri-, tetra-, penta- and hexanucleotide motifs were identified, with an occurrence frequency of 23.32%. The most abundant type was dinucleotide repeat motifs, accounting for 49.19% of the total repeat types. The mitochondrial genome, as a byproduct of GSS, was assembled to investigate the evolutionary relationships between O. evermanni and its relatives. Bayesian inference (BI) phylogenetic tree inferring from concatenated 12 protein-coding genes (PCGs) showed complicated relationships among Ophichthidae species, indicating a polyphyletic origin of the family. The results would achieve more thorough genetic information of snake eels and provide a theoretical basis and reference for further genome-wide analysis of O. evermanni.

The effects of Ni availability on H2 production and N2 fixation in a model unicellular diazotroph: The expression of hydrogenase and nitrogenase

AbstractWe investigated the effects of Ni concentration on hydrogen (H2) net production and nitrogen (N2) fixation rates in a model unicellular diazotrophic cyanobacterium, Cyanothece. We quantified NiFe uptake hydrogenase of the diazotroph to examine how Ni deficiency affects the expression of hydrogenase and nitrogenase and N2 fixation rates. We found that Ni availability controls its H2 net production and the expression of HupL, the major subunit of the uptake hydrogenase. Low Ni availability induced low HupL expression levels while yielding high H2 net production, and vice versa. We also identified the threshold range of Ni concentrations that may differentiate low and high H2 net production patterns in the diazotroph. However, the substantial variations in Ni availability and H2 net production did not affect nitrogenase expression and N2 fixation rates in Cyanothece. From two independent studies in the Atlantic tropical and subtropical oceans, we found that dissolved Ni concentrations and H2 oversaturation percentage exhibited a statistically significant negative correlation, which is consistent with the findings of this culture study. Overall, these results indicate that Ni availability may affect H2 net production and concentrations in the euphotic zone of the oceanic regions. H2 concentrations may be used as a promising proxy to reflect N2 fixation activities in tropical and subtropical oceans only under Ni‐deficient conditions.