Reynolds number (Re), pore water pressure (P), and water flow shear force (τ) are primary indicators reflecting the characteristics of subsurface flow. Exploring the calculation of these parameters will facilitate the understanding of the hydrodynamic characteristics in different subsurface flows and quantify their differences. Hence, we conducted a study to monitor soil water content, matrix potential, and pore water pressure in two typical soil profiles (with and without fissures). The distribution of Re, P, and τ in both matrix flow (MF) and preferential flow (PF) were calculated with an improved calculation method, focusing on their energy changes. Results showed that these hydrologic parameters are quite different between MF and PF. Re values in MF remained below 0.1, indicating lower water flow velocities, while the Re values ranged from 0.8 to 2 in PF, indicating higher flow velocities. The P values in PF was tens to hundreds of times higher than that in MF, which is mainly due to the rapid accumulation and leakage of water within soil fissures. Additionally, the larger hydraulic radius and gradient in PF also resulted in higher τ values in PF (2~6 N m−2) than in MF (0~1.5 N m−2). In PF, the pressure potential was the significant factor for τ, while τ in MF was dominated by the matrix potential and varies with the magnitude of the matrix potential gradient. This study suggests that Re, P, and τ could be considered as the major indexes to reflect dynamic characteristics of subsurface flow.