Substrate Vibrations Research Articles

Vibrations from the crypt: Investigating the possibility of vibrational communication in burying beetles

AbstractCommunication is fundamental in the animal kingdom, essential to interactions such as mating, defense, and parental care. Vibrational communication has often been overlooked in the past, but in recent decades, it has become clear that insects use substrate vibrations as a communication signal. In burying beetles of the genus Nicrophorus, which are known for their biparental brood care, both parents stridulate. Spending a considerable period of their lives underground, it is very likely the beetles utilize vibrations as part of their communication system. As playback experiments are challenging with this species, this study looked at the physical propagation of the signal of Nicrophorus vespilloides Herbst (Coleoptera: Siliphidae) through three soil types, as well as behavior, to see whether vibrational communication is possible. The aims were to determine: (1) whether the soils used in the laboratory compare to soil from the field, (2) whether the distance of propagation is enough for the range the beetles cover during brood care, (3) whether the two sexes show a difference in stridulation likelihood, (4) whether propagation of defensive signals differs from brood care signals, and (5) whether we can determine a behavior during stridulations that shows a clear and useable reaction to the signal. We manipulated beetles to induce stridulation and then used laser Doppler vibrometers to record the signals using three substrates and various distances, alongside behavioral observations. We showed that the three substrates tested, peat, coconut coir, and forest soil, displayed differences in terms of vibrational propagation, and that burying beetle stridulation signals can be transmitted up to about 25 cm in the soil. We also showed that the location where the animals stridulate exerts a significant influence on the total duration and number of stridulations. Overall, vibrational communication is in principle conceivable in this species, as the signals are transmitted far enough in the natural substrate to allow complex communication, opening possibilities for vibrational communication during this biparental brood care.

Parametric oscillations of the sessile drop

Waves are formed on the surface of a sessile drop driven through substrate vibrations oriented at a slanting angle from the normal. A mathematical model is derived, which leads to an infinite system of coupled Mathieu equations governing the wave dynamics that are solved using Floquet theory. The spatial structure of the waves is described by the mode number pair $[\ell,m]$ , where $\ell$ and $m$ are the polar and azimuthal mode numbers, respectively. Limiting cases corresponding to horizontal and vertical vibrations are discussed with predictions agreeing well with prior literature. We focus our results on three drop motions – (1) harmonic $[1,1]$ rocking mode, (2) harmonic $[2,0]$ pumping mode, and (3) subharmonic rocking $[1,1]$ mode – as they depend upon the slanting angle, static contact angle, and contact-line conditions, which we assume to be either pinned or freely moving with fixed contact angle. New theoretical predictions are tested through experiments over a range of parameters, showing good agreement.

Mechanically evoked spike responses of pentascolopidial chordotonal organs of Drosophila melanogaster larvae.

Mechanosensitive ensembles of neurons in insects, known as chordotonal organs (COs), function in proprioception, the detection of sound and substrate vibrations. Here, we characterized the mechanical sensitivity of the lateral pentascolopidial CO (lch5) of Drosophila melanogaster larvae to establish its postulated role in proprioception. We developed a physiologically realistic method to replicate proprioceptive input to lch5 by pulling the apodeme (tendon) to which the tips of the neurons attach. We found that lch5 sensory neurons respond transiently with a short latency to the velocity component of stretch displacements and the release of stretch (relaxation). In the mechanosensory mutant inactive, lch5 has a decreased response to mechanical stimuli and a lower overall spontaneous spike rate. Finally, we simulated the input that lch5 receives during crawling and observed spike rate changes of peristaltic body contraction. We provide a characterization of proprioceptive feedback in D. melanogaster larvae and firmly establish the proprioceptive function of lch5 in larval locomotion.

Embryonic development and cranial ossification of the Japanese Aodaishō, Elaphe climacophora (Serpentes: Colubridae): with special reference to the prootic bone and auditory evolution in snakes.

Snakes show remarkably deviated "body plan" from other squamate reptiles. In addition to limb loss, they have accomplished enormous anatomical specialization of the skull associated with the pit organs and the reduction of the tympanic membranes and auditory canals in the outer ears. Despite being the most diverse group of snakes, our knowledge of the embryonic staging for organogenesis and cranial ossification has been minimal for Colubridae. Therefore, in the present observation, we provide the first embryonic description of the Japanese rat snake Elaphe climacophora. We based our study on the Standard Event System (SES) for external anatomical characters and on a description of the cranial ossification during post-ovipositional development. We further estimated the relative ossification timing of each cranial bony element and compared it with that of selected other snakes, lizards, turtles, and crocodilians. The present study shows that the relative ossification timing of the palatine and pterygoid bones is relatively early in squamates when compared to other reptiles, implying the developmental integration as the palate-pterygoid complex in this clade and functional demands for the unique feeding adaptation to swallow large prey with the help of their large palatine and pterygoid teeth. Furthermore, unlike in species with pit organs, the prootic bone of Ela. climacophora is expanded to provide articulation with the supratemporal, thereby contributing to the hearing system by detecting substrate vibration. We also demonstrate that the relative timing of the prootic ossification is significantly accelerated in colubrids compared to snakes with pit organs. Our finding suggests that the temporal changes of the prootic ossification underpin the evolution of the perception of the ground-bourne sound signals among snakes.

Is plant acoustic communication fact or fiction?

In recent years, the idea has flourished that plants emit and perceive sound and could even be capable of exchanging information through the acoustic channel. While research into plant bioacoustics is still in its infancy, with potentially fascinating discoveries awaiting ahead, here we show that the current knowledge is not conclusive. While plants do emit sounds under biotic and abiotic stresses such as drought, these sounds are high-pitched, of low intensity, and propagate only to a short distance. Most studies suggesting plant sensitivity to airborne sound actually concern the perception of substrate vibrations from the soil or plant part. In short, while low-frequency, high-intensity sounds emitted by a loudspeaker close to the plant seem to have tangible effects on various plant processes such as growth - a finding with possible applications in agriculture - it is unlikely that plants can perceive the sounds they produce, at least over long distances. So far, there is no evidence of plants communicating with each other via the acoustic channel.

Experimental Study on the Influence of Air Conveyor System Parameters on Vibration of Glass Substrate

In this paper, the method of experimental research is used to minimize the micro-vibration amplitude of 0.6 mm thick glass substrates in the air conveyor system. The optimal parameter combination of the small orifice throttling glass substrate air conveyor system is obtained as follows: orifice diameter 0.25 mm, orifice spacing 60 mm and air-film thickness 50 μm. Secondly, based on the orthogonal experimental data, the influence of the main parameters of the air conveyor system at the micro vibration of the glass substrate is analyzed by variance analysis technology. The results show that the three principal parameters of the air conveyor system have significant influence on the vibration of the glass substrate. Among them, the thickness of the air-film has the greatest influence on the vibration of the glass substrate, the diameter of the orifice has the second influence, and the spacing of the orifice has the least influence. Finally, the influence of the three key parameters of orifice diameter, orifice spacing and film thickness on the micro vibration of glass substrate in the orifice throttling air flotation support system was studied and discussed, and the relationship curves between the maximum amplitude of micro-vibration of glass substrate and the main parameters of air flotation system were obtained.

The Impact of Substrate Vibrations on Self-Alignment Accuracy in BGA/Flip-Chip Assembly

The Impact of Substrate Vibrations on Self-Alignment Accuracy in BGA/Flip-Chip Assembly

Soft wetting: Modified Cassie-Baxter equation for soft superhydrophobic surfaces

The classic Cassie-Baxter equation has been used for decades to describe the equilibrium contact angle of a droplet on a partially wetted, rough substrate and inspired numerous follow-up explorations in wetting. However, the Cassie-Baxter equation itself and its various modified forms apply mainly to rigid surfaces, where the distortion of structures under the surface tension force has not been considered. Encouraged by the trend that many natural surfaces and various modern devices are flexible, here, we study how flexibility of surface structures affects the surface wettability, namely, the equilibrium contact angle of a water droplet. Substrates decorated with soft pillar arrays are fabricated and the equilibrium contact angles of water droplets are measured by substrate vibration. We found a deviation between the measured equilibrium contact angles and those predicted by the classic Cassie-Baxter equation, and the deviation is more pronounced for softer pillars. By incorporating the elastic energy in the deflected pillars along the droplet perimeter into the droplet-structure interfacial system, we develop a modified Cassie-Baxter equation for droplets on soft pillar arrays.

Nonlinear frequency analysis of porous Bi directional functionally graded beams utilizing reddy shear deformation theory

The nonlinear frequency response of bi-directional functionally graded porous beams experienced range of various end conditions is investigated in this work. The end conditions which are simply supported, clamped-simply supported, clamped-clamped, and clamped-free are taken by using the Von Karman geometric nonlinearity, Green's tensor and Reddy third-order shear deformation theory. A generalized differential quadrature technique (GDQM) accompanied by direct numerical iterance approach is proposed to solve equations. The findings are presented to aid in future research into the effects of various gradient indices, vibration amplitude ratios, porosity coefficients, shear and elastic substrate parameters, boundary conditions, and vibration frequencies on the bi-directional functionally graded beams. The outcomes of this research have practical applications and can be utilized to enhance the design of bi-directional beams. The results are also highly useful in anticipating and identifying potential causes of failure in these beams.

The Influence of Vibration and Moisture Content on the Compactness of the Substrate in Nursery Container Cells Determined with a Multipenetrometer

An important problem of container nurseries is ensuring equal and favorable growth conditions for cultivated plants. This can be achieved by ensuring the physical parameters of the substrate used to grow seedlings in individual cells of the container are similar. The nursery container is filled with a specially composed substrate through an automated line. Quickly controlling the parameters related to the quality of substrate filling presents a significant problem, as it requires the ongoing correction of the filling module settings (e.g., extending the vibration time or changing the vibration amplitude). To address this issue, it would be helpful to determine the compactness of the substrate, which can be easily measured using a penetrometer. This paper presents a prototype automated station, known as a multipenetrometer, designed for the simultaneous testing of compactness in 15 selected container cells. The prototype was put to the test at the Nursery Farm in Sukowo, where two types of polystyrene containers (V150—650/312/150 mm; 74 cells; and 0,148 cm3 cell volume and V300—650/312/180 mm; 53 cells; and 0.275 cm3 cell volume) were filled with peat–perlite substrate on the Urbinati Ypsilon automated line. This study investigated the influence of substrate moisture (two levels—70 and 75%) and vibration intensity (two levels—8 and 12 G) of the vibrating table on its compactness within the individual cells of the nursery container. The results indicated that with an increase in substrate moisture and vibration intensity, the compactness of the substrate increased, and the variation in compactness between individual cells decreased. Notably, the V300 containers, with a larger cell volume (265 cm3), experienced a higher level of change compared to the V150 containers (145 cm3). Despite the use of substrate compaction techniques based on the experience of line operators filling containers, the coefficient of variation between the compactness of the substrate in individual cells of the container remained at 30%. Based on the findings, it was confirmed that the optimal parameters for filling V150 and V300 containers with peat–perlite substrate on the Urbinati line, at a filling capacity of approximately 400 containers h−1, are a moisture content of around 75% and a maximum vibration intensity of 12 G.

Marine energy converters: Potential acoustic effects on fishes and aquatic invertebrates.

The potential effects of underwater anthropogenic sound and substrate vibration from offshore renewable energy development on the behavior, fitness, and health of aquatic animals is a continuing concern with increased deployments and installation of these devices. Initial focus of related studies concerned offshore wind. However, over the past decade, marine energy devices, such as a tidal turbines and wave energy converters, have begun to emerge as additional, scalable renewable energy sources. Because marine energy converters (MECs) are not as well-known as other anthropogenic sources of potential disturbance, their general function and what is known about the sounds and substrate vibrations that they produce are introduced. While most previous studies focused on MECs and marine mammals, this paper considers the potential of MECs to cause acoustic disturbances affecting nearshore and tidal fishes and invertebrates. In particular, the focus is on particle motion and substrate vibration from MECs because these effects are the most likely to be detected by these animals. Finally, an analysis of major data gaps in understanding the acoustics of MECs and their potential impacts on fishes and aquatic invertebrates and recommendations for research needed over the next several years to improve understanding of these potential impacts are provided.

Central projections of auditory nerve fibers in the western rat snake (Pantherophis obsoletus).

Despite the absence of tympanic middle ears, snakes can hear. They are thought to primarily detect substrate vibration via connections between the lower jaw and the inner ear. We used the western rat snake (Pantherophis obsoletus) to determine how vibration is processed in the brain. We measured vibration-evoked potential recordings to reveal sensitivity to low-frequency vibrations. We then used tract tracing combined with immunohistochemistry and Nissl staining to describe the central projections of the papillar branch of the VIIIth nerve. Applications of biotinylated dextran amine to the basilar papilla (homologous to the organ of Corti of mammals) labeled bouton-like terminals in two first-order cochlear nuclei, a rostrolateral nucleus angularis (NA) and a caudomedial nucleus magnocellularis (NM). NA formed a distinct dorsal eminence, consisted of heterogenous cell types, and was parvalbumin positive. NM was smaller and poorly separated from the surrounding vestibular nuclei. NM was distinguished by positive calbindin label and included fusiform and round cells. Thus, the atympanate western rat snake shares similar first-order projections to tympanate reptiles. Auditory pathways may be used for detecting vibration, not only in snakes but also potentially in atympanate early tetrapods.

The neuronal innervation pattern of the subgenual organ complex in Peruphasma schultei (Insecta: Phasmatodea)

The proximal tibia of orthopteroid insects contains sensory organs, the subgenual organ complex, detecting mechanical stimuli including substrate vibration. In stick insects, two chordotonal organs occur in close proximity, the subgenual organ and the distal organ, which likely detect substrate vibrations. In most stick insects, both organs are innervated by separate nerve branches. To obtain more data on the neuroanatomy of the subgenual organ complex from the New World phasmids (Occidophasmata), the present study documents the neuronal innervation of sensory organs in the subgenual organ complex of Peruphasma schultei, the first species from Pseudophasmatinae investigated for this sensory complex. The innervation pattern shows a distinct nerve branch for the subgenual organ and for the distal organ in most cases. Some variability in the innervation, which generally occurs for these chordotonal organs, was noted for both organs in P. schultei. The most common innervation for both organs was by a single nerve branch for each organ. The innervation of the subgenual organ resembled the nerve pattern of another New World phasmid, but was simpler than in the Old World phasmids (Oriophasmata) studied so far. Therefore, the peripheral neuronal innervation of sensory organs could reflect phylogenetic relationships and provide phylogenetic information, while the overall neuroanatomy of the subgenual organ complex is similar in stick insects.

Microscopic theory of spin relaxation of a single Fe adatom coupled to substrate vibrations

Understanding the spin-relaxation mechanism of single adatoms is an essential step towards creating atomic magnetic memory bits or even qubits. Here we present an essentially parameter-free theory by combining \textit{ab-initio} electronic and vibrational properties with the many-body nature of atomic states. Our calculations account for the millisecond spin lifetime measured recently on Fe adatoms on MgO/Ag(100) and reproduce the dependence on the number of decoupling layers and the external magnetic field. We show how the atomic interaction with the environment should be tuned in order to enhance the magnetic stability, and propose a clear fingerprint for experimentally detecting a localized spin-phonon excitation.

Functional Diversity of Vibrational Signaling Systems in Insects.

Communication by substrate-borne mechanical waves is widespread in insects. The specifics of vibrational communication are related to heterogeneous natural substrates that strongly influence signal transmission. Insects generate vibrational signals primarily by tremulation, drumming, stridulation, and tymbalation, most commonly during sexual behavior but also in agonistic, social, and mutualistic as well as defense interactions and as part of foraging strategies. Vibration signals are often part of multimodal communication. Sensilla and organs detecting substrate vibration show great diversity and primarily occur in insect legs to optimize sensitivity and directionality. In the natural environment, signals from heterospecifics, as well as social and enemy interactions within vibrational communication networks, influence signaling and behavioral strategies. The exploitation of substrate-borne vibrational signaling offers a promising application for behavioral manipulation in pest control. Expected final online publication date for the Annual Review of Entomology, Volume 68 is January 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

The effect of substrate vibration on Ag nanoparticle formation on SiO2 via thermally-induced dewetting: A molecular dynamics study

Thermally-induced metallic film dewetting on a substrate has huge advantages in paralleled nanofabrication. Although various regulation method of the dewetting process have been investigated, a mobile supporting substrate has been rarely considered. This work investigates how the vertical vibration of a SiO2 substrate affects the thermally-induced silver (Ag) film dewetting by molecular dynamics (MD) simulation. It is revealed that the substrate vibration can significantly promote the dewetting, which dramatically decreases the characteristic dewetting time. This principle of dewetting promotion remains for a tested range of film thickness from 0.2 nm to 0.6 nm. During the substrate vibration, the inertance of Ag film amplifies the film instability and thus promote the dewetting. For a 0.4 nm thick Ag film, the MD simulation shows that appropriate vibration period (T) and vibration amplitude (A) to achieve the promoted dewetting are approximately 2 ∼ 100 ps and 0.1 ∼ 0.4 Å, respectively.

Field Evaluation of Promising Indigenous Entomopathogenic Fungal Isolates against Red Palm Weevil, Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae).

The rate of the sounds (i.e., substrate vibrations) produced by the movement and feeding activity of red palm weevil (RPW) pest infestations in a date palm tree was monitored over time after trees were separately treated with injection of entomopathogenic fungal isolates, Beauveria bassiana and Metarhizium anisopliae, or water treatment as the control. The activity sensing device included an accelerometer, an amplifier, a digital recorder, and a signal transmitter that fed the data to a computer that excluded background noise and compared the rates of bursts of movement and feeding sound impulses among treated trees and controls. Observations were made daily for two months. The rates of bursts were representative of the feeding activity of RPW. The unique spectral pattern of sound pulses was typical of the RPW larval feeding activity in the date palm. The microphone confirmed that the same unique tone was produced in each burst. Two months after fungal injection, the RPW sound signal declined, while the RPW sound signal increased in the control date palms (water injection). The mean rates of bursts produced by RPW decreased to zero after the trees were injected with B. bassiana or M. anisopliae compared with the increased rates over time in the control treatment plants.

A trapdoor spider, Latouchia typica (Araneae: Halonoproctidae), uses vibrational cues as a trigger for predatory behavior

AbstractSpiders are one of the most dominant predators in terrestrial ecosystems. Although cues triggering predatory behavior in web‐building and wandering spiders are well investigated, studies concerning burrowing species, the most ancestral group of spiders, are relatively limited. To clarify critical cues affecting the predatory behavior in burrowing species, we conducted vibration‐reducing experiments with the trapdoor spider Latouchia typica (Araneae: Halonoproctidae) and nymphs of the speckled cockroach, Nauphoeta cinerea (Blattodea: Blaberidae), as prey. Spiders achieved a high success rate of prey capture even when blinded (with paint on the eyes). However, the use of a rubber mat to reduce vibrations significantly decreased predation success rate. In addition, the presence or absence of the blindfold did not affect the predation rates under the reducing vibration condition. These results indicate that substrate vibrations emitted from prey are critically important to trigger the predatory behavior in L. typica, but visual and chemical stimuli are not used even in the case when vibration cues are unavailable. This is the first study to use vibration‐reducing experiments to experimentally demonstrate the critical cues for predation in trapdoor spiders.

Morphological coupling of the distal organ in the Peruvian walking stick (Oreophoetes peruana): Structural and functional aspects

AbstractIn insects, the detection of mechanical stimuli from body movements, airborne sound, substrate vibration, medium flow, or gravity by mechanosensory organs plays an important role. These mechanosenory organs can have complex morphologies with numerous sensilla, and the functional morphology with specific attachments of the sensory neurons to surrounding tissues and structures determines the stimulation. In stick insects, the subgenual organ complex in the tibia of all legs is an elaborate system of two chordotonal organs, which respond to substrate vibrations, and associated tibial campaniform sensilla, which respond to cuticular strain. One chordotonal organ, the distal organ, is characterized by a linear set of sensilla. This distal organ has not been studied for its physiological characteristics in detail, but the attachment or mechanical coupling is functionally important. Here we characterize two aspects of attachment or mechanical coupling of the distal organ: At the dorsal side, the organ is connected to the inner side of the dorsal cuticle by connective tissue, which is shown to also contain the axons of campaniform sensilla. At the proximal end, a fine membrane runs to the adjacent chordotonal organ, the subgenual organ. This membrane spans the tibia in transverse direction. It does not contain neuronal elements, but as a connection between the subgenual and the distal organ, it may influence the mechanosensory activity of these organs. Such a connection is not present in other insects such as locusts or cockroaches and could affect the sensory function in stick insects (e.g., in vibration detection by the subgenual organ) or even couple the two organs, resulting in similar mechanical responses.

Oscillations of Drops with Mobile Contact Lines on the International Space Station: Elucidation of Terrestrial Inertial Droplet Spreading.

We analyze shape oscillations of sessile water drops with fully mobile contact lines (CL) aboard the International Space Station. The unique microgravity environment enables the study of centimeter-sized droplets with associated inertial-capillary motions. Plane-normal substrate vibrations induce resonance behaviors quantified by frequency scans from which the natural frequencies and mode shapes are identified for nine different hydrophobic surfaces. Experimental observations agree well with, and validate, a recent spectral prediction of mobile CL sessile drop oscillations. The experimental findings help elucidate terrestrial droplet inertial spreading, a poorly understood phenomenon pervasive in many processes.