Abstract Date biomass is a renewable natural resource since it can be substituted in a comparatively shorter period. Hence it is considered as a possible feedstock for bioenergy motivations through anaerobic digestion (AD). Despite the fact AD is a fully demonstrated technology, the use of new feedstock necessitates detailed investigation. Worldwide several researchers are evaluating methane yield short of paying much consideration to the source type and structural and elemental composition of biomass. Hence, the current study was intended to relate methane yield to those two structural and chemical composition of biomass. In this sense, biochemical methane potential (BMP) for different date biomass, namely Pedicels, Fibrilium, Petiole, Fruit bunch, Spath, Palm, and a mixture of all biomass samples was tested for 30 days. Among all the experimental variations, higher productivity was observed with Palm as 72.60 mL CH4/g VS/days, followed by Spath (66.61 mL CH4/g VS/days), Mixed biomass (64.57 mL CH4/g VS/days), Fruit bunch (64.34 mL CH4/g VS/days), Pedicels (59.06 mL CH4/g VS/days), Fibrilium (57.42 mL CH4/g VS/days), and Petiole (41.17 mL CH4/g VS/days). From the experimental results, it can be concluded that among all the biomass samples, Palm is the best substrate for higher methane production. However, the remaining experimental conditions produced methane yield was comparatively low. This might be due to the composite nature of the substrate with lignin as an insoluble fraction. Hence, Pedicels, Fibrilium, and Petiole biomasses cannot be recommended as a substrate for biogas production without appropriate pretreatment.