This paper designs an electronic substation simulation relay protection device with load check. At the same time, the device is studied and applied in the new substation. First of all, this paper analyzes the primary wiring mode and three-phase symmetrical current and voltage in the primary side of a typical substation, and confirms the transformer ratio, polarity and wiring mode of the current/voltage transformer. Then, by checking the polarity of line protection, metering, bus protection, main variable differential protection and backup protection, the polarity error of metering circuit with load check protection and metering circuit is effectively avoided. Finally, through the field test verification in the new 220kV Fuxing station of Jinzhou power supply company, it is verified that the technology adopted in this paper can fully meet the requirements of substation simulation load test. The relay-protection device with load check designed in this paper solves the problem that the conventional electric-type single-phase large-current generator can only detect the transformer ratio and polarity of the relative single-phase loop current transformer, and the difference loop cannot be detected. It shortened the switching operation time of changing ratio, polarity and phase check of the primary and secondary circuits after passing the load of the substation transmission belt.