Plant Substances Research Articles

Oxalic acid-assisted phytoextraction of heavy metal contaminated wastewater through Lemna minor L.

The present research was carried out to investigate the performance of oxalic acid (OA) through Lemna minor L. (duckweed) in the phytoextraction of lead (Pb) from metal contaminated water. Zero, 100 μM, 250 μM, and 500 μM Pb concentration and combinations with 2.5 ml of OA were provided to the plants in the form of solution after defining intervals. Continuous aeration was provided to the plants and kept a pH level at 6.5. Results from this research depicted that increasing concentration level of Pb inhibited the overall plant growth, biomass, frond area, chlorophyll, and antioxidant enzyme activities like peroxidase (POD), superoxide-dismutase (SOD), catalases (CAT), and ascorbate-peroxidase (APX). Moreover, Pb stress enhances the concentration, hydrogen peroxide, malondialdehyde, and electrolyte leakage substances in plants. Furthermore, the addition of OA alleviated the Pb-induced toxicity in the plants, increasing the Pb accumulation and its endorsement in the L. minor. The OA addition increased the Pb accumulation in plants at 0, 100, 250 and 500 μM. At higher concentration, Pb showed harmful effect as related to the other low doses. Under the application of OA, Pb higher accumulation and concentration in L. minor were measured, which showed that it could be the most suitable plant for the phytoextraction of lead-contaminated soil and wastewater.

Influence of collagen films on the microbial obsemination of semi-finished meat products

The article shows the effect of collagen films on microbial contamination and quality indicators during the storage of semi-finished products, as well as microbiological and organoleptic studies. Innovations in the field of food packaging are currently the most promising breakthrough of nanotechnology in everyday life. The impact of nanotechnology on the life, health and safety of mankind in the coming century can be compared with the general impact of antibiotics, printed circuits and polymers on the life of society. According to the results of microbiological studies, it was found that the use of film barrier coatings leads to inhibition of the growth of microflora and has a bacteriostatic effect, which contributes to lengthening the shelf life of semi-finished products compared with control samples. The research works were devoted to semi-finished beef products. The study revealed the dynamics of the microflora content of semi-finished products during storage, studied the effect of the developed collagen coatings on the development of microorganisms and changes in the quality indicators of meat semi-finished products during long-term storage. And also, an organoleptic assessment of the quality of packaged products was carried out. The aim of the work is to substantiate the barrier technologies of meat semi-finished products and culinary products due to the purposeful use of biologically active substances of plants and collagen proteins of animal tissues.

XII All-Russian Scientific Conference with international participation and school for young scientists “Chemistry and Technology of Plant Substances”

XII All-Russian Scientific Conference with international participation and school for young scientists “Chemistry and Technology of Plant Substances”

The formation of a phytopathogenic microbiome as a factor of biological pollution of agrocoenoses of vivas

The formation of the phytopathogenic microbiome as a factor in the biological contamination of oat agrocenoses is an important research task, the solution of which will make it possible to create ecologically balanced agroecosystems. This will increase their ability to self-regulate the population of micromycetes in order to obtain high-quality and safe oat raw materials. Therefore, the influence of environmental factors (abiotic, biotic, anthropogenic) on the formation of micromycete populations in the oat leaf microbiome under different plant growing technologies was studied. The article presents the results of ecological assessment of oat plant varieties based on indicators of influence on population density, frequency of occurrence, and intensity of sporulation of micromycetes. Vegetative organs of oat plants of Parlamentskyi and Tembr varieties were selected in the following phases: tillering, emergence into tubes and earing. It was determined that climatic conditions, as an abiotic factor, namely an increase in air temperature, frequent droughts, rare but abundant rains, which changed depending on the year of the study, significantly influenced the formation of micromycete populations in the oat leaf microbiome. Plant growing technologies, as an anthropogenic factor, significantly influenced the spectrum of species and their frequency of occurrence in the leaf microbiome of oats of various varieties. Under the organic technology of growing plants, the spectrum of micromycete populations was more diverse, but with a lower frequency of occurrence of species compared to the traditional technology of growing plants. Also, varieties of oat plants, as a biotic factor, thanks to the physiological substances of plants, are able to restrain the spread of micromycete populations in the leaf microbiome of plants or stimulate them. It was found out that with the traditional and organic technology of growing plants in the leaf microbiome of Tembr oat variety, the population density, the frequency of occurrence of micromycete species, and the intensity of sporulation were significantly lower compared to Parlamentskyi oat variety plants. This indicates that the cultivation of oat varieties capable of restraining the formation of micromycete populations at an ecologically safe level will ensure a decrease in the level of biological pollution of agrocenoses and increase the biosafety of plant raw materials

Метаболические изменения в организме кур-несушек при применении антибиотика и фитобиотика

Abstract. The use of medicinal plant extracts in animal production has recently increased due to the discovery of previously unreported side effects of antibiotics, including the cumulation of their toxic metabolites in food, as well as the increased occurrence of antibiotic resistance of microorganisms. Non-therapeutic use of antimicrobials is also associated with the spread of multiple drug resistance, including resistance to antimicrobials that have never been used in animal agriculture. It has long been known that components of plant substances have the ability to inhibit bacterial growth. It is becoming common practice to use plant essential oils to improve feed utilization by ruminants, and essential oils have long been known for their antimicrobial properties. The purpose of this work was to evaluate the metabolic effects of phytobiotic and joint use with antibiotic in controlling microbial load. According to the results obtained, it is clear that phytobiotic in addition to antimicrobial effects has other positive effects expressed in membrane stabilizing and antioxidant properties, but there are some negative effects associated with difficulty of digestion and absorption of lipid nutrients. At the same time, the combined use of phytobiotic and antibiotic showed itself as optimal, due to the good antimicrobial effect, while mitigating or eliminating the negative effects identified when using phytobiotic and enrofloxacin separately, namely, eliminating immunosuppressive and depressive effects, as well as the absence of manifestations of impaired digestion and absorption of fat-soluble nutrients. Scientific novelty. The data obtained will expand the range of antimicrobial agents used in poultry farming, as well as allow the use of more flexible schemes to control the microbial load on the body of birds.

Evaluation of modern enterosorbents: possibilities and prospects

Absolutely all preclinical studies involve three groups of experiments: preclinical studies of the pharmacokinetics of the substance, including toxicokinetics, preclinical studies of the pharmacokinetics of the drug and the clinical study of pharmacokinetics on target animals. Pharmacokinetic studies of native drugs are complex because they usually involve the administration of complex components. Thus, only the use of the whole complex of pharmacognostic, chemical, spectral, physicochemical and biological methods can provide an objective assessment of the authenticity and quality of medicinal herbal raw materials and medicinal herbal preparations. The study used Wistar white rats, weight 150-160g according to the Guidelines for Evaluation of Medicinal Products (3). The purpose of the study was to evaluate the pharmacokinetic properties of a new feed additive in the form of a premix of sorbents and phytocompositions: milk thistle (DV-silibinin, Pharmcenter VILAR CJSC), thyme (DV-timol, Akhma100). Supplements in the form of finished dosage forms were standardized for the active substance, and have a constant composition. Thus, it allowed to create a study design for a new substance in one animal species. Since the composition of the sorption component was studied earlier, the introduced substances were considered separately and in combination with sorbents. After a single injection, the linearity of the pharmacokinetics was assessed, based on the administered different doses, the analysis of the main biochemical parameters of the blood was carried out, and the bioavailability was also calculated. Thus, the new premix based on sorbents and plant substances in the form of milk thistle and thyme does not have a negative effect on the body of laboratory animals. As a result of the studies, LD50 and LD100 were not established for the drug, the drug is classified as low-toxic, belongs to the fourth hazard class according to GOST 12.1.007-76 "Harmful substances", which allows further toxico-therapeutic tests.

Chromobacterium Violaceum: A Model for Evaluating the Anti-Quorum Sensing Activities of Plant Substances

In the new antibiotic era, the exponential increase in multiresistant bacterial strains has become the main global health problem. Many researchers have focused their efforts on exploring novel or combined strategies for combating bacterial resistance. Good knowledge of the molecular mechanisms of resistance and bacterial virulence factors as key targets provides us with a good basis for resolving the problem. One particularly attractive and promising strategy is to attack the main regulatory “network” of bacterial virulence determinants known as quorum sensing (QS). The inhibition of QS signals will be a novel means of screening more effective quorum-sensing inhibitors (QSIs) and will play a key role in the use of next-generation antimicrobials in the battle against resistance. This motivated the present review to provide a comprehensive clarification of the regulatory mechanisms of quorum-sensing signaling pathways in Chromobacterium violaceum and the discovery of potential plant quorum-sensing inhibitors.

Per‐ and polyfluoroalkyl substances in foam and dewatering streams at wastewater treatment plants

AbstractThe occurrence of per‐ and polyfluoroalkyl substances (PFAS) in wastewater treatment plants (WWTPs) is well recognized. While several studies have examined the occurrence of PFAS in WWTPs, studies assessing the extent to which unit processes impact PFAS phase distribution are relatively lacking. Herein, PFAS enrichment in foams generated during aeration and PFAS accumulation in solids dewatering streams were evaluated in WWTPs. Results of this screening‐level study showed that perfluorooctane sulfonate (PFOS) concentrations were enriched up to three orders of magnitude in foams relative to the aqueous phase. Similarly, total PFAS concentrations in solids dewatering streams were enriched up to 380 times relative to the aqueous influent. The PFAS mass in the dewatering stream was a significant component of the overall PFAS mass exiting the WWTP at one of the facilities examined. Results of this study suggest that there is potential to mitigate PFAS by improved management of these foams and dewatering waste streams.

Psychoactive substances in soils, plants, freshwater and fish: A mini review

Psychoactive substances in soils, plants, freshwater and fish: A mini review

OPTIMAL CONDITIONS FOR CLEANING AND DRYING FUROSTANOL SAPONINS FROM TRIBULUS TERRESTRIS

Furostanol saponins from Tribulus terrestris may be separated from ballast substances by liquid-liquid extraction with changing polarity of the extractant, depending on the dynamics of the process. Treatment with chloroform removed the maximum number of lipid-like compounds and showed minimal losses of furostanol saponins, and subsequent treatment with ethyl acetate retrieved low-polarity substances. Fourfold extraction with butanol was used to extract furostanol saponins from an aqueous solution. Optimal conditions were developed for spray drying of furostanol saponins. The greatest yield of “Dry extract Tribulus” was obtained with a solution feed rate of 80 l/h, a spray head rotation speed of 8000 rpm, and a heat carrier velocity of 2000 kg/h. Technology was developed for obtaining “Dry extract Tribulus” from Tribulus terrestris that contained at least 45% furostanol saponins. The reproducibility of the developed technology was shown with the obtaining of 5 series of substances that meet the requirements of regulatory and technical documentation on the lines of the Scientific and Technological Center for GMP Requirements the Institute of Chemistry of Plant Substances.

Occurrence of per- and polyfluorinated alkyl substances in wastewater treatment plants in Northern Italy

Wastewater treatment plants are known to be relevant input sources of per- and polyfluoroalkyl substances (PFAS) in the aquatic environment. This study aimed to investigate the occurrence, fate, and seasonal variability of twenty-five PFAS in four municipal wastewater treatment plants (WWTP A, B, C, and D) surrounding the city of Milan (Northern, Italy). Composite 24-h wastewater samples were collected in July and October 2021 and May and February 2022 from influents and effluents of the four WWTPs. PFAS were detected at concentrations ranging between 24.1 and 66.9 μg L−1 for influent and 13.4 and 107 μg L−1 for effluent wastewater samples. Perfluoropentanoic acid was the most abundant (1.91–30.0 μg L−1) in influent samples, whereas perfluorobutane sulfonic acid predominated (0.80–66.1 μg L−1) in effluent samples. In sludge, PFOA was detected in plant A at concentrations in the range of 96.6–165 ng kg−1 dw in primary sludge samples and 98.6–440 ng kg−1 dw in secondary treatment sludge samples. The removal efficiency of total PFAS varied between 6 % and 96 %. However, an increase of PFAS concentrations was observed from influents to effluents for plant D (during July and October), plant A (during October and May), and plant C (during May) indicating that biotransformation of PFAS precursors can occur during biological treatments. This was supported by the observed increase in concentrations of PFOA from primary to secondary treatment sludge samples in plant A. Moreover, the plant operating at shorter hydraulic retention times (plant D) showed lower removal efficiency (<45 %). Seasonal variation of PFAS in influent and effluent appears rather low and more likely due to pulse release instead of seasonal factors.

Concentration-dependent mechanisms of fluoranthene uptake by ryegrass

Fluoranthene (Flu) uptake by plants is affected by plant growth and environmental concentration. Although plant growth processes, including substance synthesis and antioxidant enzyme activities, have been reported to regulate Flu uptake, their contributions have been poorly evaluated. Moreover, the effect of Flu concentration is little known. Here, low concentrations (0, 1, 5, and 10 mg/L) and high concentrations (20, 30, and 40 mg/L) of Flu were set to compare the changes in Flu uptake by ryegrass (Lolium multiflorum Lam.). Indices of plant growth (biomass, root length, root area, root tip number, and photosynthesis and transpiration rates), substance synthesis (indole acetic acid [IAA] content), and antioxidant enzyme activities (superoxide dismutase [SOD], peroxidase [POD], and catalase [CAT]) were recorded to unravel the mechanism of Flu uptake. Findings suggested that the Langmuir model fitted Flu uptake by ryegrass well. Flu absorption capacity in the root was stronger than that that in the leaf. Flu bioconcentration and translocation factors increased then reduced with the increase in Flu concentration and reached the maximum value under 5 mg/L Flu treatment. Plant growth and IAA content had the same pattern as before bioconcentration factor (BCF). SOD and POD activities increased then decreased with Flu concentration and reached their highest levels under 30 and 20 mg/L Flu treatments, respectively, whereas CAT activity decreased continuously and reached its lowest level under 40 mg/L Flu treatment. Variance partitioning analysis indicated that IAA content had the greatest significant effect on Flu uptake under low-concentration Flu treatments, whereas antioxidant enzyme activities had the greatest significant effect on Flu uptake under high-concentration Flu treatments. Revealing the concentration-dependent mechanisms of Flu uptake could provide a basis for regulating pollutant accumulation in plants.

The evaluation of chitosan hydrogel based curcumin effect on DNMT1, DNMT3A, DNMT3B, MEG3, HOTAIR gene expression in glioblastoma cell line.

Cancer is one of the most important causes of death worldwide. Some types of cancer, including glioblastoma, with a high potential for growth, invasion, and resistance to general treatments, chemotherapy, and radiotherapy, have a high potential for recurrence. Many chemical drugs have been used to treat it, but herbal drugs are more effective with fewer side effects; Therefore, this research aims to investigate the effect of curcumin-chitosan nano-complex on the expression of MEG3, HOTAIR, DNMT1, DNMT3A, DNMT3B genes in the glioblastoma cell line. In this research, glioblastoma cell line, PCR and spectrophotometry techniques, MTT test and transmission, field emission transmission, and fluorescent electron microscopes were used. The morphological examination of the curcumin-chitosan nano-complex was without clumping, and the fluorescent microscope examination showed the nano-complex enters the cell and affects the genes expression. In its bioavailability studies, it was found that it significantly increases the death of cancer cells in a dose- and time-dependent manner. Gene expression tests showed that this nano-complex increased MEG3 gene expression compared to the control group, which is statistically significant (p < 0.05). It also decreased HOTAIR gene expression compared to the control group, which was not statistically significant (p > 0.05). It decreased the expression of DNMT1, DNMT3A, and DNMT3B genes compared to the control group, which is statistically significant (p < 0.05). By using active plant substances such as curcumin, the active demethylation of brain cells can be directed to the path of inhibiting the growth of brain cancer cells and eliminating them.

Perfluoroalkyl substances in Romanian wastewater treatment plants: Transfer to surface waters, environmental and human risk assessment

The current study aimed to determine the concentration levels of nine perfluoroalkyl substances (PFAS) in the five most significant Romanian wastewater treatment plants (WWTPs) and their transfer to natural receivers. The analytes were concentrated using a solid-phase extraction/ultrasonic-assisted extraction procedures and then selectively quantified by liquid chromatography–tandem mass spectrometry (LC–MS/MS) using electrospray ionization. In most of the wastewater samples investigated, the dominant compounds were perfluoropentanoic acid (PFPeA), perfluorooctanoic acid (PFOA), and perfluorooctansulfonate acid (PFOS), with the maximum concentration range between 105 and 316 ng/L in influents, 14.8–31.3 ng/L in effluents and removal efficiencies higher than 80 % for all selected PFAS compounds. In sewage sludge samples, PFOA and PFOS were the majority substances, with values up to 35.8 ng/g dw for PFOA and 27.8 ng/g dw for PFOS. PFOA and PFOS achieved the maximum levels by estimating mass loading and emission levels. As a result, 237 mg/day/1000 people of PFOA and 95.5 mg/day/1000 people of PFOS are entering daily in the WWTPs, while up to 31 mg/day/1000 people of PFOA and up to 13.6 mg/day/1000 people of PFOS are discharged daily into the natural emissaries. Human risk assessment shows that PFOA and PFOS are low to high risk for all age and gender categories. Children are the most exposed to PFOA and PFOS contamination in drinking water. Environmental risk assessment reveals that PFOA exhibits a low risk for some insect species, PFOS - a low risk for freshwater shrimps and a medium risk for midges, while perfluoroundecanoic acid (PFNuDA) could have a low to medium risk for midges. No assessment studies regarding the environmental and human risk of PFAS have been performed in Romania.

Comparative Analysis of Quality of Essential Oil of Wormwood Herb Synanthropic Flora of Voronezh Region

Introduction. The formation and accumulation of biologically active substances in plants is a complex process associated with a number of environmental factors, including anthropogenic ones. The study of the characteristics of the qualitative composition of essential oil of wormwood grass of bitter different from the ecological point of view of the places of harvesting is relevant.Aim. The aim of this study study of the qualitative composition of essential oil of bitter wormwood grass, harvested in areas of the Voronezh region that are different from an ecological point of view.Materials and methods. In the Voronezh region, 4 points of raw materials procurement were selected, diverse in terms of anthropogenic impact. Isolation of essential oil from the raw materials was carried out according to the method of PS "Wormwood of bitter grass". Component composition of the obtained essential oils was determined using Agilent 7890B GC System (Agilent Technologies, USA) with Agilent 5977A MSD mass selective detector (Agilent Technologies, USA). Data analysis and processing was carried out on the basis of NIST11 databases (from 19.05.2011), MassHunter ver. B.06.00 and NIST MS Search ver. 2.0 software were used.Results and discussion. In a sample of essential oil obtained from reserved raw materials, monoterpene compounds account for more than 82 %, and sesquiterpene compounds – 16.6 %, about 2 % are organic impurities. Intensive biosynthesis of monoterpenes was noted in a sample collected in the area of agricultural fields of the Verkhnekhavsky district, its mass fraction is more than 73 %. In samples of raw materials with anthropogenic load (OJSC "Minudobreniya" and Highway M4), the share of monoterpene compounds is significantly lower (63.7 and 49 %, respectively). The increase in the proportion of sesquiterpene compounds in the last samples of wormwood essential oil may be due to the excessive acidity of the urbanized places of the workpiece. The sesquiterpene compound hamazulene, which stains essential oil blue, is identified in only two samples of gorse grass wormwood harvested along agricultural fields and in M4 tracks, which explains the presence of a blue hue in these oils.Conclusion. The chromato-mass spectrometric analysis of the essential oil of the studied samples of bitter wormwood grass made it possible to identify more than 70 different components in them, while the qualitative composition of the essential oil of the raw materials of different places of the workpiece was significantly different, which may indicate a significant influence of the place of growth of the species and anthropogenic factors on the features of the secondary metabolism of terpene compounds in the plant organism.

Bioinformation Search for Biologically Active Substances in Coniferous Plants of the Picea Genus Distribution in the Urals

In coniferous plants of the Picea Genus, common in the Urals, using a bioinformatic approach, 12 biologically active substances were selected, in which potential biological activity was evaluated. The analyzed biologically active substances belong to terpenes and terpenoids, they are divided into 2 groups, which, along with others, have antibacterial and anti-inflammatory activities. BAS with antibacterial activity were identified as promising: canfen, α-pinene, β-myrcene, trans-caryophyllene, borneol, manool. Bioinformatics analysis is only the first step in the study of biologically active substances in coniferous plants.

Investigation of the polyphenol composition of red oak (Quercus rubra L.) raw materials

The aim of the work was to determine the main groups of polyphenolic compounds in Quercus rubra L. raw materials.&#x0D; Materials and methods. The leaves and annual shoots of Northern Red Oak used for research were collected in August in Lisnyky village of Obuhiv district of Kyiv Oblast (Ukraine). Determination of the component composition and quantitative content of flavonoids (including separately catechins), hydroxycinnamic acids, and phenolic acids were carried out in the samples of air-shade-dried crushed raw material to a particle size of 3 mm by the method of high-performance liquid chromatography (HPLC). Agilent Technologies 1200 liquid chromatograph was used for liquid chromatography.Results. Using the HPLC method, 18 polyphenolic compounds were identified in leaves and annual shoots of Northern Red Oak, in particular, flavonoids: rutin, quercetin-3-β-glucoside, luteolin, neohesperidin; catechins: catechin, epicatechin, epicatechin gallate, gallocatechin; hydroxycinnamic acids: chlorogenic, caffeic, trans-ferulic, trans-cinnamic, p-coumaric, hydroxyphenylacetic, benzoic, syringic, sinapic acids; phenolic acid is gallic acid. The dominant component among flavonoids is rutin (323.43 mg/100 g) (in the composition of catechins, epicatechin gallate (25.45 mg/100 g) prevails); among hydroxycinnamic acids in Northern Red Oak raw materials, chlorogenic acid (139.62 mg/100 g) and sinapic acid (74.64 mg/100 g) prevail.Conclusions. The obtained results point to the prospects of further phytochemical and pharmacological studies of Quercus rubra raw materials, with the aim of creating new plant substances based on it with antioxidant, anti-inflammatory, and antiviral activity

Research progress of proanthocyanidins and anthocyanidins.

Proanthocyanidins (PA) are polyphenol compounds that are widely distributed in the bark, fruit core, skin, or seeds of various plants. Anthocyanidins are water-soluble natural pigments widely found in plants. They are all flavonoids, a major coloring substance in plants and fruits. In recent years, research into PA and anthocyanins has become increasingly popular because of their excellent anti-oxidation, scavenging of reactive oxygen free radicals and other physical and chemical activities, and their anti-cancer, vision protection, aging prevention, skin beauty pharmacological, and nutraceutical effects. Especially, recent systematic reviews and meta-analyses indicate their value, safety, and efficacy in the prevention, adjuvant therapy, and management of cardiometabolic disease. Here, we summarize their research progress from the aspects of chemical structure, biosynthetic pathways, distribution, extraction and separation, coloration, efficacy, and potential. The comparison between them might provide a reference for their development and efficient utilization. However, more large-sample-size randomized controlled trials and high-quality studies are needed to firmly establish their clinical efficacy.

Raman spectroscopy in crop quality assessment: focusing on sensing secondary metabolites: a review.

As a crop quality sensor, Raman spectroscopy has been consistently proposed as one of the most promising and non-destructive methods for qualitative and quantitative analysis of plant substances, because it can measure molecular structures in a short time without requiring pretreatment along with simple usage. The sensitivity of the Raman spectrum to target chemicals depends largely on the wavelength, intensity of the laser power, and exposure time. Especially for plant samples, it is very likely that the peak of the target material is covered by strong fluorescence effects. Therefore, methods using lasers with low energy causing less fluorescence, such as 785nm or near-infrared, are vigorously discussed. Furthermore, advanced techniques for obtaining more sensitive and clear spectra, like surface-enhanced Raman spectroscopy, time-gated Raman spectroscopy or combination with thin-layer chromatography, are being investigated. Numerous interpretations of plant quality can be represented not only by the measurement conditions but also by the spectral analysis methods. Up to date, there have been attempted to optimize and generalize analysis methods. This review summarizes the state of the art of micro-Raman spectroscopy in crop quality assessment focusing on secondary metabolites, from in vitro to in vivo and even in situ, and suggests future research to achieve universal application.

Analysis of the pharmacokinetic properties of a native drug on the basis of a complex of sorbents

Absolutely all preclinical studies involve three groups of experiments: preclinical studies of the pharmacokinetics of the substance, including toxicokinetics, preclinical studies of the pharmacokinetics of the drug and the clinical study of pharmacokinetics on target animals. Pharmacokinetic studies of native drugs are complex because they usually involve the administration of complex components. Thus, only the use of the whole complex of pharmacognostic, chemical, spectral, physicochemical and biological methods can provide an objective assessment of the authenticity and quality of medicinal herbal raw materials and medicinal herbal preparations. The study used Wistar white rats, weight 150-160g according to the Guidelines for Evaluation of Medicinal Products (3).The purpose of the study was to evaluate the pharmacokinetic properties of a new feed additive in the form of a premix of sorbents and phytocompositions: milk thistle (DV-silibinin, Pharmcenter VILAR CJSC), thyme (DV-timol, Akhma100). Supplements in the form of finished dosage forms were standardized for the active substance, and have a constant composition. Thus, it allowed to create a study design for a new substance in one animal species. Since the composition of the sorption component was studied earlier, the introduced substances were considered separately and in combination with sorbents.After a single injection, the linearity of the pharmacokinetics was assessed, based on the administered different doses, the analysis of the main biochemical parameters of the blood was carried out, and the bioavailability was also calculated. Thus, the new premix based on sorbents and plant substances in the form of milk thistle and thyme does not have a negative effect on the body of laboratory animals. As a result of the studies, LD50 and LD100 were not established for the drug, the drug is classified as low-toxic, belongs to the fourth hazard class according to GOST 12.1.007-76 "Harmful substances", which allows further toxico-therapeutic tests.