Substances In Dissolved Organic Matter Research Articles

Bacteria Affect the Distribution of Soil-Dissolved Organic Matter on the Slope: A Long-Term Experiment in Black Soil Erosion

Soil erosion results in dissolved organic matter (DOM) loss and is one of the main paths of soil carbon loss. Bacteria affect the generation and transformation of DOM. However, the effect of bacteria on the composition and slope distribution of DOM has rarely been investigated under field conditions. Based on a long-term experiment of three gradients (3°, 5°, 8°) in a black soil erosion area of Northeast China, the content, composition, and source of DOM were studied. The results showed that the DOM of the 3° and 5° slope was enriched midslope, and the DOM of the 8° slope was enriched downslope. Parallel factor (PARAFAC) analysis indicated that the main substances in DOM were fulvic-like acid, humic-like acid, tryptophan-like protein, and soluble microbial metabolites. The upslope and downslope soils of 3° and 5° slopes showed high DOM bioavailability, while the downslope soil of the 8° slope showed high DOM bioavailability. The content of new DOM in downslope soil increased with the gradient. Bacteria played an important role in the synthesis and transformation of DOM and affected its composition and slope distribution. Verrucomicrobiota, Firmicutes, Planctomycetota, and Gemmatimonadota were the main factors affecting soil DOM. The results could be helpful in understanding the loss mechanism of DOM in eroded black soil and provide support for soil carbon sequestration.

Study on the mechanism of co-pyrolysed biochar on soil DOM evolution in short-term cabbage waste decomposition

Cabbage waste returned soil was studied to assess the short-term influences of the application of cabbage waste biochar (CB), pine wood biochar (PB), and co-pyrolysed biochar (PCB) on soil dissolved organic matter (DOM) evolution. The decrease in DOM and soil organic matter (SOM) content was greater in the biochar-added soils during 35 days of decomposition. The DOM and SOM content in PCB added group decreased by 26.96 mg L−1 and 4.48 g kg−1, respectively. The increase in relative abundance of humic acid-like substances in DOMs was higher in the biochar-added soils during decomposition, which increased by 4.29% in PCB added group. PCB addition also resulted in a high SOM content (initial content of 78.82 g kg−1), and mineral elements were introduced into the soil, thus increasing soil pH (7.81) and electrical conductivity (574.67 μs cm−1). Moreover, the addition of biochars attenuated the decrease in average relative abundance of Bacillaceae and promoted bacterial proliferation during decomposition. The application of biochars regulated the soil bacterial community and promoted organic matter conversion and soil DOM evolution.

Component Properties and Heavy Metal Accumulation Characteristics of Contaminated Rice Straw Biochar During Oxygen-controlled Pyrolysis

Pyrolysis is an important technology to achieve the harmlessness and recycling of contaminated biomass. In this study, the effects of oxygen-controlled atmosphere on the component properties and heavy metal accumulation characteristics of contaminated rice straw biochar were studied. The results showed that low-oxygen pyrolysis could effectively produce biochar using contaminated rice straw and improve the stability of heavy metals in biochar. Under the nitrogen atmosphere, the yield of rice straw biochar was 29.4%-34.9%. The aromatization index (SUVA254) of dissolved organic matter (DOM) increased first and then decreased with the increase in pyrolysis temperature, whereas the fluorescent components were mainly humic-like acid substances. Meanwhile, Ca mainly existed in the form of CaCO3 in biochar. Compared with the pure nitrogen condition, the biochar yield was reduced by 5.6%-13.5% and 14.9%-15.7% under the pyrolysis atmosphere containing 10% and 20% oxygen content, respectively. Ca existed in the form of CaO in biochar, which increased the pH value of the biochar by more than 0.5 units. The oxygen of the pyrolysis atmosphere accelerated the degradation of the lignin component, resulting in the gradual decrease in SUVA254 of DOM. With the increase in oxygen content in the pyrolysis atmosphere, humic-like acid substances in DOM were transformed into fulvic-like acid substances. Under the conditions of 400℃ and a 10% oxygen-containing atmosphere, the exchangeable fractions of Cu, Cd, Pb, Ni, and As in biochar were decreased by 5.2%, 3.7%, 1.7%, 0.8%, and 0.7%, respectively, indicating that heavy metals are transformed into more stable states. The results suggested that the higher biochar yield and heavy metal stability could be obtained by introducing a proper amount of nitrogen into the air (controlling the oxygen content of approximately 10%) for pyrolysis treatment of contaminated rice straw, providing an economic and feasible technology for the achievement of harmlessness and recovery of contaminated rice straw.

Changes in soil dissolved organic matter optical properties during peatland succession

• Topsoil and subsoil DOM respond differently to aboveground vegetation succession. • Succession increases the content of humic-like substances in topsoil DOM. • The content of protein-like material of the subsoil is stable under succession. • pH is a key factor affecting changes in fluorescence properties of topsoil DOM. Dissolved organic matter (DOM) is the most active component in the soil environment. Understanding the chemical diversity of DOM and the interaction of the physicochemical properties of the soil are key to managing peatland under succession. In this study, we aimed to understand the effects of peatland succession on soil DOM. We collected soil samples (topsoil: 0–10 cm, subsoil: 10–20 cm) from peatland during different stages of peatland succession in mid–high latitude northern regions, determined the changes in quantity and quality of DOM using fluorescence spectroscopy and parallel factor analysis. We found that peatland succession altered the effectiveness of soil nutrients and water content. During the succession, the content of humus-like substances in the DOM of the topsoil increased and the content of protein-like material decreased, whereas the content of substances in the subsoil remained stable. pH was the key factor affecting the change in the composition of the DOM in the topsoil during peatland succession. The variation of DOM in the subsoil may be related to the vegetation composition. The results suggest that fluorescent DOM components respond significantly to changes in peatland succession, and DOM properties are driven by soil pH and vegetation composition during peatland succession. In conclusion, our results reveal the optical changes and factors that influence DOM in peatlands under succession. This suggests that DOM can be modified by simultaneous changes of the physicochemical properties in the soil and the vegetation cover.

Characteristics of dissolved organic matter in urban road runoff under different traffic densities in Beijing, China.

The characteristics of dissolved organic matter (DOM) in road runoff under different traffic densities were compared using resin fractionation, molecular weight distribution analysis, three-dimensional excitation-emission matrix fluorescence spectroscopy, and UV-visible spectroscopy. Heavy traffic density strongly increased the concentration of DOM in road runoff, resulting in higher dissolved organic carbon. The distribution of molecular weights in DOM was not influenced by the traffic. DOM was dominated by colloidal organic matter with molecular weight in the 1kDa to 0.45μm size range. Hydrophobic fractions accounted for a large proportion than hydrophilic fractions in DOM, and hydrophobic acids increased with higher traffic density. Traffic density did not alter the fluorescent substances in DOM, including fulvic-like UV fluorescent substances and protein-like substances. However, more tryptophan-like protein substances were found in DOM from road runoff with heavy traffic. Moreover, the aromatization degree of DOM was not affected by heavy traffic, while the degree of humification decreased.

Insight into structural composition of dissolved organic matter in saline-alkali soil by fluorescence spectroscopy coupled with self-organizing map and structural equation modeling

Soil salinization has been occurring all over the world, which severely affected crop production and threatened the life of mankind. It is necessary to take serious steps to improve soil fertility for the sustainability and productive capacity of agriculture. Soil samples of different depths were collected from native vegetation communities (Comm. Phragmites communis (CPC) and Comm. Populus alba (CPA)) and irrigated crops (corn fields (CFD) and seed melon fields (SMF)) in Hetao irrigation area of China. Three dimensional excitation-emission matrix (EEM) fluorescence technology combined with self-organizing map were used to analyze the dissolved organic matter (DOM) composition and structural characteristics in saline-alkali soils and its spatial distribution under different vegetation covers. Critical factors were recognized by classification and regression tree (CART) for distinguishing soil samples, and latent factors were revealed with structural equation modeling (SEM) for improving the humification degree of DOM from saline soils in Hetao irrigation area. Five components were obtained in the DOM substances, i.e., tyrosine-like (C1), tryptophan-like (C2), UV fulvic-like (C3), visible fulvic-like (C4) and humic-like (C5). The protein-like peaks were all obvious, and the fulvic-like peaks (600-735 a.u.) were conspicuous in the CPC soil than in others, except CFD1 and SMF1. C1 was the critical factor to distinguish native vegetation from irrigated crops, and C1 and C2 were the critical factors to distinguish CFD from SMF. Contrary to the HA/FA (0.20) and A/C (0.25), the path coefficient (-0.15) of sources with T/H was negative, indicating that the incremental contents of fluorenscense substances were in the sequences of protein-like > visible fulvic-like > UV fulvic-like > humic-like, affecting by the allochthonous. C1 (1.00) and C4 (1.00) were the primary components for improving the humification degree of DOM, which were principally originated from plant debris. EEM combined with self-organizing map, CART and SEM is an efficient way to distinguish different salinized soils and reveal the latent factors for improving the soil fertility.

Insight into the effect of wastewater-derived dissolved organic matter composition on norgestrel degradation in activated sludge: Coupled bacterial community and molecular characteristics

Dissolved organic matter (DOM) mediates the microbial transformation of micropollutants, including norgestrel (NGT) in natural waters. However, little is known of the effect of complex and variable wastewater-derived DOM composition on NGT degradation during wastewater treatment. In this study, the relationship between the compositions of initial DOM and NGT removal efficiencies of 17 wastewater treatment plants (WWTPs) in spring and summer were analyzed. The different molecular composition of DOM was selected in the lab to further explore its effect on NGT degradation by activated sludge. Results indicated that the DOM composition was a substantial driver of NGT removal in WWTPs. The discrepancies in the initial DOM composition contributed to the differences in the kinetics of NGT degradation by activated sludge. The larger rapid decay phase rates of NGT are usually accompanied by a large proportion of labile substances in DOM. High-throughput sequencing and ultrahigh-resolution mass spectrometry were used to further analyze the evolution of bacterial communities and DOM molecular composition were combined with network analysis to reveal the intrinsic relationship that how DOM composition affected NGT degradation by regulating core microbes. Eighty-nine core OTUs were significantly associated with NGT degradation, and 73 occurred in the rapid decay phase, implying that NGT degradation was mainly regulated by the initial composition of DOM. Nine major transformation products were identified in different groups with widely varying concentrations or relative abundances of these transformation products. This work provides valuable insights into the effects of wastewater-derived DOM composition on NGT degradation by activated sludge and innovatively explores the influence mechanisms from the bacterial community and molecular characterization perspectives.

Dissolved organic matter (DOM) was detected in MSWI plant: An investigation of DOM and potential toxic elements variation in the bottom ash and fly ash

The content of dissolved organic matter (DOM) and potentially toxic elements (PTEs) were investigated in the bottom ash (BA) and fly ash (FA) of different sections of the municipal solid waste incineration (MSWI) plant. BA and FA were collected from the dry (BA1-BA2), burn (BA3-BA4), and burn-out (BA5) sections of the grate incinerator; FA was collected after denitration (DNFA), and from the deacidification tower (FA1) and bag-type dust remover (FA2), respectively. The DOM concentration in BA was higher than that in FA, the highest concentration was in BA3 (556.18 mg/kg), while the lowest concentration was in DNFA (17.53 mg/kg). DOM in BA was mainly composed of protein-like, fulvic-like, tryptophan-like, and humic-like substances, of which humic-like substances accounted for more than 40%. DOM in FA consisted of tryptophan-like and humic-like substances, of which humic-like substances accounted for more than 80%. DOM still existed in BA which may be related to the incomplete combustion, and the influence of microbes, while DOM was increased in FA1, which might be due to the addition of lime slurry. PTEs were analyzed by the Tessier extraction method, Fe-Mn hydroxide-bound fraction of PTEs increased in FA1 in which DOM concentration (137.22 mg/kg) was 7.83 times that in DNFA. The increase of DOM may lead to a higher risk of PTEs in FA. FTIR results indicated that DOM can bond to PTEs in BA and FA. The contents of humus-like substances in DOM were positively correlated with the effective fraction of As, Cu, Pb, Cr, and Cd. This paper investigated the risk of DOM existing in BA and FA in MSWI plant, which can provide a new perspective on how to deal with BA and FA, and reduce their environmental risks.

Sorption of Pb(II) onto biochar is enhanced through co-sorption of dissolved organic matter

Biochar plays an important role in controlling migration of pollutants in soils. However, little information is available on the interactions between soil-derived dissolved organic matter (DOM), biochar and soluble metal species. The aim of this work was to present the adsorption process of soil DOM by biochar (corn straw biochar produced at 700 °C) and to determine whether co-sorption of DOM would change the affinity for Pb(II). The adsorption rates of biochar and biochar + DOM for Pb(II) were best fitted with a pseudo-second order kinetic model, and the equilibrium adsorption isotherm data agreed well with both the Langmuir and Freundlich models. Adsorption of DOM to biochar reached equilibrium after 15 h with an uptake of 52% of the supplied DOM. We used fluorescence excitation-emission matrices (EEMs) with parallel factor (PARAFAC) analysis to demonstrate that protein-like, fulvic acid-like and humic acid-like substances were the primary constituents of the DOM, which were quenched over time in the presence of biochar. Synchronous fluorescence spectra indicated that the protein-like structures were the predominant fluorescence substances in DOM. Two-dimensional correlation spectroscopy (2D-COS) showed the binding of DOM to biochar resulted in the quenching of fluorescence in the order: protein-like substances > humic-like substances (280 > 355 nm). Data supports the notion that DOM can increase the adsorption capacity of biochar for metal-ions.

Different Responses of Soil Dissolved Organic Matter to Different Types of Compost

This study explored the responses of soil dissolved organic matter (DOM) to the application of different types of compost using a soil sample without compost as a control. Ultraviolet and fluorescence spectrum technology and EEM-PARAFAC was used to analyze DOM structure and driving factors in soil added with different proportion of cow dung compost (SCC), food and kitchen waste compost (SFC), and sludge compost (SCC). Compared with the control group, contents of AN, NH4+-N, DOC, and SOM in soil added with compost were significantly increased, and contents of SOM and DOC increased with the increasing of compost amount. When added compost in the same proportion, contents of AN, NO3--N, and DOC in SCC and SFC were significantly higher than those in SSC, while contents of NH4+-N and SOM were higher in SSC. The results of spectral analysis showed that the structure of conjugated benzene ring, hydrophobic component, quinone group, and chromogenic component in DOM of soil added with compost were significantly increased, the transition of unsaturated organic molecule (π→π*) was more active, the molecular weight of DOM increased, and the degree of humification was enhanced. When the amount of compost added is 5%, the influence of food and kitchen waste compost on DOM structure was greatest among three types of compost. At 10% and 20%, sludge compost had the greatest impact on DOM structure. The results of EEM-PARAFAC analysis showed that the relative content of fulvic acid-like substances with low molecular in DOM of soil added with compost was increased, while the relative content of proteoid-like substances decreased. 2D-COS analysis showed that compost affected the change order of fluorescence components in DOM. SCC and SFC were as follows:proteoid-like > fulvic acid-like > humus-like; in SSC, it was fulvic acid-like > proteoid-like > humus-like. The enhance of humification and the decrease of relative content of protein-like substances in DOM were related to increased DOC and AN, the relative content of humus-like in low molecular weight was positively correlated with the content of NO3--N, and the relative content of macromolecule fulvic acid-like was increased due to the input of SOM from compost.

Spatial and temporal comparisons of dissolved organic matter in river systems of the Three Gorges Reservoir region using fluorescence and UV–Visible spectroscopy

Understanding optical characteristics, composition and source of dissolved organic matter (DOM) in rivers of the Three Gorges Reservoir (TGR) region is important for region and global carbon cycle. However, chemical compositions and source of DOM from the tributary to mainstream in the TGR region are not well studied. Consequently, 126 water samples were collected from rivers in different land use region, and these rivers covered the main tributaries of the Yangtze River in the TGR region. The temporal and spatial variations of DOM structure and source identification in different land use region were investigated using UV–visible absorbance and fluorescence spectroscopy. Overall, there were higher ratio of humic acid to fulvic, aromaticity, molecular weight and proportion of colored humic substances in DOM in the wet season than in the dry season. The weaker biologic/microbial and stronger terrestrial sources in DOM were observed in the wet season than in the dry season. DOM comparison in variable land use demonstrated the higher terrestrial sources and weaker biologic/microbial sources in DOM in the forest-affected rivers irrespective of hydrological seasonality, as well as in the wet season irrespective of land use types. DOM in the farmland-affected rivers showed more protein-like signal. We found that monsoonal precipitation, anthropogenic activities and land use were important drivers for the DOM quality variations. These findings will be beneficial to unravel riverine DOM structure and sources in relation to anthropogenic activities and also improve our understanding of DOM biogeochemical cycle in the rivers.

The ultraviolet-visible absorbance and fluorescence characterization of dissolved organic matter derived from the leaf litter of Populus simonii, Artemisia desertorum, Salix cheilophila, and Populus tomentosa.

Dissolved organic matter (DOM) derived from leaf litter plays an important role in maintaining carbon (C) and nitrogen (N) circulation between soils and plants, energy flow, and signaling pathways for plant-microbe interactions of terrestrial ecosystem. In this study, four DOM samples extracted with a 40:1 (v/w) water to sample ratio from the leaf litter of Populus simonii (S1), Artemisia desertorum (S2), Salix cheilophila (S3), and Populus tomentosa (S4) were investigated using the technologies of ultraviolet-visible (UV-Vis) and excitation-emission matrix (EEM) fluorescence spectroscopy. Results showed that the electricity (EC) values of four DOM extracts were significantly different due to the different composition and salt content of each plant. The values of chemical oxygen demand (COD), dissolved organic carbon (DOC), and the sum of values of all peaks' intensities divided by DOC (FI) indicated the higher contents of organic matter in the acid DOM extracts from S1, S2, and S3 (sand-fixing plants) than the neutral DOM extracted from S4. The absorbance shoulder between 250 and 285nm in the UV-Vis spectra and EEM fluorescence spectra of each sample suggested the presence of many different chromophores such as aromatic or phenolic compounds in plant DOM. According to fluorescence regional integration (FRI) and peak picking results, the content of protein-like materials was higher than that of humic-like substances in DOM from S1, S2, and S3 while the opposite phenomena occurred in DOM from S4. Hence, the physicochemical and fluorescence characterization of DOM extracted from the genus Populus of the family Salicaceae S1 and S4 growing under different edaphic and climatic conditions changed much. The findings would be of great significance to understand the origin, composition, dynamics, and biotransformation of DOM in soils formed in different climatic environments.

Effects of moisture and salinity on soil dissolved organic matter and ecological risk of coastal wetland

Coastal wetland is the transitional area between land and ocean, which has a unique and sensitive ecosystem. In this study, the effects of moisture and salinity on dissolved organic matter (DOM) and adsorption of heavy metal ions (Cr(VI), Cd(II) and Pb(II)) by soil are investigated. Meanwhile, ecological risks for the potential release of N, P and heavy metals are also predicted. UV–Vis spectrophotometry and three-dimensional fluorescence spectroscopy are used to study the content and structural of DOM under different soil moisture and salinity. Soil adsorption of heavy metal ions is determined by inductively coupled plasma (ICP). The results show that soil moisture and salinity have significant effects on the basic physical and chemical properties of soil. DOM content is the highest in medium moisture and high salinity areas. In addition, the content of protein-like substances in DOM is the highest under all treatment conditions. The results also reveal that the increase of DOM promotes Cr(VI) adsorption and inhibits Cd(II) adsorption by soil. When Pb(II) concentration is high (150 mg/L), the increase of DOM inhibits Pb(II) adsorption by soil. The comprehensive ecological risk of heavy metals is the highest under high salinity. The potential release risk of N and P is the lowest at high moisture and low moisture, respectively. Base on above, effects of soil moisture and salinity on the surrounding ecological environment in coastal wetlands have been revealed, which provides a theoretical basis for the protection of coastal wetland ecological environment.

The role of dissolved organic matter in soil organic carbon stability under water erosion

Dissolved organic matter (DOM) can respond rapidly to external interference and is of great importance to soil carbon cycling. However, little information is available about the characteristics of DOM in response to water erosion and its role in soil organic carbon (SOC) stability. To contribute this area, a typical watershed in the Southern Red Soil Hilly Region was studied and soil samples were collected from two contrasting sites (eroding versus depositional sites). The DOM concentration and spectral characteristics, SOC stability and their relationships were determined. Results showed that DOM concentration in the deposited topsoil (0–5 cm, 0.69 g kg−1) was significantly higher than that in the eroded soil (0.27 g kg−1). Dissolved organic matter rich in aromatic, hydrophobic and high molecular weight moieties were concentrated in the eroded topsoil rather than in the deposited topsoil. Three fluorescent components in DOM were finally identified: tryptophan-like (C1), humic-like (C2) and protein-like (C3) components. The humic-like C2 in the eroded topsoil was significantly higher than that of the deposited topsoil (P < 0.05). The largest cumulative CO2-C (23.29 mg CO2-C kg−1 soil d−1) was found in the deposited soil of 0–5 cm and it was approximately three times as high as that of the eroded soil. Regression analyses showed that the cumulative CO2 was significantly and positively related to the DOM concentration in two sites, and remarkably negatively correlated with the abundance of aromatic, hydrophobic, high molecular weight and humic-like substances in DOM. Our results indicated that the DOM was strongly affected by water erosion and can be an effective indicator for predicting the SOC stability feedback to erosion.

Leachate from fine root litter is more acidic than leaf litter leachate: A 2.5-year laboratory incubation

Some tree species increase fine root production under soil acidification, thus changing the balance of litter input from leaves and roots. Litter leaches a significant amount of acidic materials during its decomposition, which might facilitate soil acidification. In this context, we focused on dissolved organic matter (DOM) as the major component of acidic materials. We hypothesized that both the quality and quantity of DOM, which control its function (i.e., proton supply), differ between leaf and root litter. To test this hypothesis, we conducted a 2.5-year laboratory incubation experiment using fresh fine roots and fresh green leaves as litter of two coniferous species (Cryptomeria japonica and Chamaecyparis obtusa) and investigated the leachate pH and DOM composition based on the optical properties. After the early stage of decomposition when flash leaching of DOM converged, the amount of dissolved organic carbon (DOC) leached from roots increased again and leachate pH declined. In contrast, DOC concentrations continued to decrease in leaf leachates during the incubation period, and the pH decrease was not as striking as that of root leachates. Optical properties (ultraviolet visible absorption and fluorescence) of DOM revealed that humic-like substances in DOM played a central role in the acidic pH of root leachates. The total amount of protons released from roots of C. japonica and C. obtusa is about 13 and 18 times higher, respectively, than that from leaves. These results imply that the increase of fine root biomass may induce a positive plant–soil feedback in acidic soils, affecting soil biogeochemical functions of terrestrial ecosystems.

Composition, Evolution, and Complexation of Dissolved Organic Matter with Heavy Metals in Landfills

Samples of wastes and leachates were collected from a landfill site and a leachate treatment plant[i.e., equalization basin, anaerobic zone, oxidation ditch, and membrane bioreactor (MBR) section]. Dissolved organic matter (DOM) was extracted from the wastes and leachates, and its composition, evolution, and complexation characteristics with heavy metals were studied using UV-Visible and fluorescence spectroscopy combined with1H nuclear magnetic resonance. The aliphatic compounds were found to be the main substances in DOM in the fresh landfill wastes (<5 a), and the relative content of aromatics and substituent groups, i.e., carbonyl, hydroxyl, and carboxyl functional groups, decreased during the initial process. On the other hand, carbohydrates and organic amines were observed to be the main substances in DOM obtained from the intermediate and old landfill wastes (>5 a), and the relative content of aromatics and substituent groups (carbonyl, hydroxyl, and carboxyl functional groups) increased persistently during the process of organic matter humification. The aliphatics, carbohydrates, and organic amines all existed in DOM from the equalization basin Carbohydrates and aromatic compounds increased rapidly after the anaerobic, aerobiotic, and membrane treatment. However, low molecular weight organic matter and alkyl chain substances decreased during the leachate treatment process and the side chain of the aliphatics was shortened despite the increase in its content. The distribution of zinc in the wastes and leachates was influenced by the complexation with the nitrogen-and oxygen-containing functional groups, whose effect on other metals was not obvious.

Chemotaxis toward phytoplankton drives organic matter partitioning among marine bacteria

The microenvironment surrounding individual phytoplankton cells is often rich in dissolved organic matter (DOM), which can attract bacteria by chemotaxis. These "phycospheres" may be prominent sources of resource heterogeneity in the ocean, affecting the growth of bacterial populations and the fate of DOM. However, these effects remain poorly quantified due to a lack of quantitative ecological frameworks. Here, we used video microscopy to dissect with unprecedented resolution the chemotactic accumulation of marine bacteria around individual Chaetoceros affinis diatoms undergoing lysis. The observed spatiotemporal distribution of bacteria was used in a resource utilization model to map the conditions under which competition between different bacterial groups favors chemotaxis. The model predicts that chemotactic, copiotrophic populations outcompete nonmotile, oligotrophic populations during diatom blooms and bloom collapse conditions, resulting in an increase in the ratio of motile to nonmotile cells and in the succession of populations. Partitioning of DOM between the two populations is strongly dependent on the overall concentration of bacteria and the diffusivity of different DOM substances, and within each population, the growth benefit from phycospheres is experienced by only a small fraction of cells. By informing a DOM utilization model with highly resolved behavioral data, the hybrid approach used here represents a new path toward the elusive goal of predicting the consequences of microscale interactions in the ocean.

Spectral Exploration of Calcium Accumulation in Organic Matter in Gray Desert Soil from Northwest China.

Little attention has been paid to the accumulation of soil organic matter (SOM) in the fringes of the mid-latitude desert. In this paper, soil samples from a long-term field experiment conducted from 1990 to 2013 at a research station in Urumqi, China by different fertilizer treatments, were used to determine soil properties and soil dissolved organic matter (DOM) by chemical analysis, fluorescence excitation emission matrix (EEM) spectroscopy, and high resolution-transmission electron microscopy (HR-TEM). The binding features of DOM under the addition of Ca2+ were analyzed using a two-dimensional (2D) Fourier transform infrared (FTIR) spectrometer further to explore the response of the DOM to increasing concentrations of Ca2+. Long-term application of chemical fertilizers and goat manure increased soil organic carbon (SOC) by 1.34- and 1.86-fold, respectively, relative to the non-fertilized control (8.95g.kg-1). Compared with the control, application of chemical fertilizers and manure significantly increased the concentrations of Ca, Mg, Si, humic and fulvic acid-like substances in DOM but decreased the amounts of trivalent metals (Al and Fe) and protein-like substances. Although crystalline Al/Fe nanoparticles and amorphous or short-range-order Si/Al nanoparticles existed in all DOM samples, crystalline Ca/Si nanoparticles were predominant in the samples treated with goat manure. Although organic matter and Si-O-containing nanoparticles were involved in the binding of Ca2+ to DOM, application of chemical fertilizers weakened Ca2+ association with components of the amide II group (1510 cm-1) and Si-O linkage (1080 cm-1), whereas application of goat manure enhanced the affinity of Ca2+ for Si-O linkage. Our results suggested that the enrichment of Ca in gray desert soil possibly helps accumulate SOM by forming crystalline Ca/Si nanoparticles in addition to Ca2+ and organic matter complexes.

Study on UV–Visible Spectra Characteristic of Dissolved Organic Matter during Municipal Solid Waste Composting

This paper aims to characterize the evolution of organic matter during the composting of municipal solid waste (MSW). During the 46-day composting process, six samples with different composting time were collected from Songjiang Solid Waste Biological Treatment Plant in Shanghai, China. Dissolved organic matter (DOM) was extracted by ultra-pure water, and the UV-Visible (UV-Vis) spectra of DOM were scanned and investigated. The results showed that, during the composting process, non-humus substances in DOM were gradually transformed into humus substances, the aromatization, molecular weight and humification of humus substances increased. Correlation analysis showed that the absorbance value at 280 nm (SUVA280), and the area of 226~400 nm (A226~400) correlated positively with each other. The dissolved organic carbon (DOC) concentration exhibited a negative correlation with the parameters S275~295, SUVA280 and A200~226, and the correlation coefficients between the DOC content and the A220~226 value was the best (r= -0.976, P=0.001). The absorbance ratio bewteen 465 and 665 nm (E465/E665) showed a significant correlation with SUVA280 (r=-0.892, P=0.017), A226~400 (r=-0.909, P=0.012) and DOC (r=0.840, P=0.036). The absorbance ratio of 250 to 365 nm (E250/E365) correlated significantly with DOC (r=-0.880, P=0.021), and showed no correlation with the other parameters. Absorption curve slope in the range of 275~295nm (S275~295) showed a significantly positive correlation with the DOC content (r=0.961, P=0.002). Among all parameters, the A200~226, A226~400 and S275~295 values reflected compost maturity best.

Quantitative characterization of Cu binding potential of dissolved organic matter (DOM) in sediment from Taihu Lake using multiple techniques

Dissolved organic matter (DOM) plays an important role in heavy metal speciation and distribution in the aquatic environment especially for eutrophic lakes which have higher DOM concentration. Taihu Lake is the third largest freshwater and a high eutrophic lake in the downstream of the Yangtze River, China. In the lake, frequent breakout of algae blooms greatly increased the concentration of different organic matters in the lake sediment. In this study, sediment samples were collected from various part of Taihu Lake to explore the spatial difference in the binding potential of DOM with Cu. The titration experiment was adopted to quantitatively characterize the interaction between Cu(II) and DOM extracted from Taihu Lake sediments using ion selective electrode (ISE) and fluorescence quenching technology. The ISE results showed that the exogenous DOM had higher binding ability than endogenous DOM, and DOM derived from aquatic macrophytes had a higher binding ability than that derived from algae. The fluorescence quenching results indicated that humic substances played a key role in the complexation between DOM and Cu(II) in the lake. However, because of the frequent breakout of algae blooms, protein-like matters are also main component like humic matters in Taihu Lake. Therefore, the metals bound by protein-like substances should be caused concern as protein-like substances in DOM were unstable and they will release bound metal when decomposed.