Substances Of Origin Research Articles

Analysis of the State Pharmacopoiea’s of the Russian Federation requirements’ coverage of drug products and drug substance of different origin and drug products included in the list of vital and primary drug products

Analysis of the State Pharmacopoiea’s of the Russian Federation requirements’ coverage of drug products and drug substance of different origin and drug products included in the list of vital and primary drug products

A Concise Diastereoselective Total Synthesis of α-Ambrinol

(-)-cis-α-Ambrinol is a natural product present in ambergris, a substance of marine origin that has been highly valued by perfumers. In this paper, we present a new approach to its total synthesis. The starting material is commercially available α-ionone and the key step is an intramolecular Barbier-type cyclization induced by CpTiCl2, an organometallic compound prepared in situ by a CpTiCl3 reduction with Mn.

What’s new in the field of serum-based eye drops

Worldwide serum-based eye drops are successfully used in the treatment of the Dry Eye Syndrome (DES). Autologous serum eye drops (ASEDs) are most common but allogenic eye drops were introduced in many countries mostly because of the COVID-19 pandemic. Not all aspects of the product formula have however been worked out so far. Each medical entity involved in the preparation, packaging and distribution of the product performs according to its own procedures. Various actions have therefore been undertaken to standardize, harmonize and exchange the experience in this field. In 2022 the 5th edition of EDQM’s Guide to the quality and safety of tissues and cells for human application was issued. Within the EU4Health Programme a new group for Recommendations and Guidance Documents for the Management of Substances of Human Origin (SoHO) in Hospitals is being formed. International cooperation of serumbased product users lead to the establishment of working group events — Workshops on the Eye Drops of Human Origin (EDHO) and Serum eye drop manufacturer’s group. Despite the widespread use of serum-based eye drops all over the world, in almost none of the countries, EU included, are there strict legal regulations for handling the product. It is therefore necessary to come up with a universal definition of artificial tears therapy and to determine specific procedures for the product preparation.

Hygiene products for udder health of lactating cows

The introduction of the “Milking Protocol” on the farm is a mandatory condition for preventing intramammary infections, maintaining cows' productivity, and improving milk quality. Treating udders with hygiene products is an effective preventive udder care measure in most dairy herds. The choice of an antiseptic agent for udder health should be based on its proven effectiveness, which is necessary to register the drug as a veterinary medicinal product. The purpose of the study was to get acquainted with the current literature on the use of hygiene products for the health of the udder of lactating cows. While writing this review article, we got acquainted with the results of modern scientific research, conducted their analysis and theoretical justification, and summarized the data obtained on using hygienic means for sanitizing the udder of lactating cows. For this purpose, the Polissia National University scientific library funds, scientific search systems Science-Direct, and PubMed were used. This means that sanitation of the mammary gland should have an antiseptic effect and keep the breasts in good condition. Declared composition of registered drugs in Ukraine: iodine, chlorhexidine, organic acids. Hygienic products include cosmetic surfactants that soften the skin and form a moisturizing hydrophilic layer on the skin. Means for udder hygiene, as a rule, are products of chemical origin, and using these drugs creates a risk of chemical elements entering the milk. An alternative to these potential dangers can be probiotic-based products that are environmentally friendly and harmless to the animal body. In the conditions of war and economic troubles, the specialists of the dairy industry are offered preparations of plant origin for the disinfection of cow udders, where medicinal plants are used as raw materials, from which infusions, decoctions, water extracts, alcohol- and oil-based solutions are prepared. Applying dipping procedures is an integral part of the preventive measures of the anti-mastitis program. The choice of means for disinfecting udders after milking must be made depending on the circulation of pathogens in the herd and consider all factors in the conditions of a specific product. In this regard, I believe that a promising direction of scientific research is the use of nanotechnology in the development of new antiseptics, the study of their effect on the health of the udder of lactating cows, the use of probiotics as a dip, as an alternative to substances of chemical origin.

Toxicological characteristics of raw milk with different contents of 17β-estradiol

Despite the benefits and biological value of dairy products, they can also be a source of dangerous substances of natural and artificial origin. Such substances include the estrogen hormone 17β-estradiol, an excessive amount of which causes oncological diseases and reproductive system dysfunction. A study was conducted to determine the toxicological evaluation of raw milk with different 17β-estradiol content on the culture of the ciliate Tetrachymena pyriformis. The content of 17β-estradiol was determined by enzyme immunoassay using the RIDASCREEN®17β-őstradiol test system (Art-Biopharm/R-Biopharm, Darmstadt, Germany). During the determination of acute toxicity, under the condition of the content of 17ß-estradiol in the raw milk in the amount from 20.0 to 2500 pg/ml, no changes in motor activity, body shape and inhibition of reproduction of ciliates were observed. At the content of 17ß-estradiol in milk in the range of 5000.0−5500.0 pg/ml, a slowdown in the motor activity of ciliates was observed without changing the nature of the movement. Thus, the amount of 17ß-estradiol in milk greater than 5000.0 pg/ml causes a minor effect on the vital activity and motor activity of Tetrachymena pyriformis ciliates. During 96 hours of the existence of ciliates in milk, chronic toxic effects on cells were not observed for the content of 17ß-estradiol from 20.0 to 2500.0 pg/ml. Addition of 17ß-estradiol to raw milk in the amount of 5000.0 to 5500.0 pg/ml caused a chronic toxic effect on ciliates, as a result of which the total score in all determined indicators increased to 2 points. Thus, raw milk with an amount of 17ß-estradiol from 5000.0 to 5500.0 pg/ml may exhibit some chronic toxic effect on the cells of the ciliated infusoria. It was established that the relative biological value of raw milk with a content of 17ß-estradiol up to 2500.0 pg/ml was from 98.6 to 97.7 %, and in samples of raw milk with the maximum amount of the hormone (5000.0−5500.0 pg/ml) decreased by an average of 10 % to 89.22 %. Therefore, if the amount of 17ß-estradiol in milk is more than 5000.0 mg/ml, a chronic toxic effect on Tetrachymena pyriformis cells is possible.

Substances of Natural Origin in Medicine: Plants vs. Cancer.

Continuous monitoring of the population's health is the main method of learning about disease prevalence. National and international data draw attention to the persistently high rates of cancer incidence. This necessitates the intensification of efforts aimed at developing new, more effective chemotherapeutic and chemopreventive drugs. Plants represent an invaluable source of natural substances with versatile medicinal properties. Multidirectional activities exhibited by natural substances and their ability to modulate key signaling pathways, mainly related to cancer cell death, make these substances an important research direction. This review summarizes the information regarding plant-derived chemotherapeutic drugs, including their mechanisms of action, with a special focus on selected anti-cancer drugs (paclitaxel, irinotecan) approved in clinical practice. It also presents promising plant-based drug candidates currently being tested in clinical and preclinical trials (betulinic acid, resveratrol, and roburic acid).

Volatomics in healthcare: technical basis and clinical application

Volatilome is a collection of all volatile compounds, both organic and inorganic, the source of which is the object under study. Unlike the metabolome, which includes only compounds of endogenous origin, the concept of volatilome includes substances of both endogenous and exogenous origin. Exhaled air volatilome contains thousands of metabolites and volatile organic compounds (VOCs), which are formed both in the respiratory tract and in the systems of internal organs and tissues. The study of the chemical composition of human exhalation can provide clinically useful information about the state of human health, while the studies are non-invasive and safe for the patient. The instrumental methods used in the study of human volatilome make it possible to online examine large numbers of patients. All this contributes to a high interest on the part of the medical community in the study of human exhaled air volatilome and suggests that the methods of these research methods have a high potential for implementation in clinical practice.

Neuroprotective effect of curcumin and its potential use in the treatment of neurodegenerative diseases

The development of methods used in molecular biology has allowed a milestone in medical and pharmaceutical sciences. Progress has also been made in the field of pharmacognosy, which places substances of natural origin contained in plant raw materials at the center of attention. The beneficial effects of some of them have been known for years, while scientific evidence of their health-promoting properties was lacking for a long time. This was also the case with curcumin and the long road from its isolation in pure form in 1842 to the knowledge of its chemical structure in 1910. Due to the chemical properties of the molecule, curcumin is attributed with many health-promoting properties. These affect many organ systems including the skin, visual pathway, respiratory system, circulatory system, digestive system and nervous system. One of the complications that follow nerve damage is the loss of locomotor function in the animal and the development of inflammation within it. Curcumin has anti-inflammatory properties. This is confirmed by its inhibition of nuclear factor κB, a mediator in inflammatory processes. In addition, a very important field associated with neuronal dysfunction is the aging process. This is caused, among other things, by the presence of reactive oxygen species. The neuroprotective effect of curcumin allows to reduce their concentration caused by the accumulation of mutations within the mitochondrial DNA. The beneficial effect on the nervous system is due to the penetration of curcumin across the blood-brain barrier. However, its poor solubility significantly limits the therapeutic properties resulting from curcumin supplementation. Methods are currently being developed to increase its bioavailability using nanoparticles.

Cannabinoid Addiction: Pharmacotherapy, Forensic and Pharmaceutical Practice, Availability of Drugs

Data from the scientific literature on the current state of the spread of dependence on psychoactive substances of plant origin – cannabinoids was processed. The problem of cannabinoid addiction has been studied. Forensic and pharmaceutical practice was analyzed. The development of organizational measures in order to provide drug patients with cannabinoid addiction (F12) with a comprehensive approach was substantiated. The peculiarities of pharmacotherapy and the availability of drugs for drug addicts have been clarified. The methods of assessing the availability and effectiveness of hepatoprotectors based on pharmacoeconomic, forensic and pharmaceutical indicators have been improved.

Scientific Overlook of Shodhan Concept in Ayurvedic Pharmaceutics: Metals and minerals

Shodhan, unique concept of purification & potentiation in Ayurvedic pharmaceutics, specially mentioned for metals and minerals which have been indicated in range of disorders from common (fever etc.) to serious (cancer etc.) disorders. Shodhan of metals and minerals is extremely depending up on its physico-chemical properties. The metals & minerals having melting point more than 5000C (Swarna-Gold, Lauha-Iron, Tamra-Copper etc) subjected to Shodhan by Nirvapa (Heating and dipping into liquid medium) whereas Dhalana (Heating, melting and dipping into liquid medium) process is applied to the substance having melting point in range of 100 to 500 0C (Naga-Lead, Vanga-Tin, Yasad-Zinc etc.). Furthermore, substances of organic origin like praval (coral), mukta (pearl), shankha (conch shell) etc. and metals/ mineral having melting point less than 100 0C like parad (mercury) is mainly subjected to shodhan either by swedan (Boiling or Fomentation with liquid) or bhavana (Trituration with small quantity of liquid) process.

Field journal as a source of information on traditional medicine (based on the materials of the expedition to the Trans-Onega region in 1931)

The interest towards the Trans-Onega region of Karelia emerged among researchers from the second half of the 19th c. at the folklore, linguistic, and ethnographic levels, although the researchers have not yet considered traditional medicine as a cultural layer of the ethnos. The problematics of the research is determined by insuffi-ciently studied traditional and non-traditional cultures of the Trans-Onega people, including ethnomedicine. In order to fill this gap, the data from a field journal reposited in the Scientific Archive of the Karelian Scientific Cen-ter of the Russian Academy of Sciences are drawn upon. When systematizing the information obtained from the field journal, scientific inquiries into traditional medicine of the Russian and Finno-Ugric peoples were utilized. The proposed research is aimed at the analysis and systematization of the materials of the field journal, which con-tains information about the traditional medicine of the Russians of the Trans-Onega region in the 1930s collected in the villages of Petry, Longasy, Garnitsy, Migury, Konda, Motalovo, Lelikovo, Koselga, Zinovievo, Sennaya Guba, etc. The research was carried out using the contrastive-comparative method. For this analysis, data were used on the rational and irrational healing practices of one of the groups of the Karelian ethnos, the Ludian Kare-lians, which is justified by their close contacts with the Russians of the Trans-Onega region. Ludian Kalelians traditionally live in a number of villages and small towns of the south-eastern part of the Republic of Karelia in Olonetsky, Pryazhinky, and Kondopozhsky Districts; hence, the settlement territory of the Ludian Karelians spans approximately 200 km in the eastern part of Olonets Isthmus, from north to south, from the Suna River to the Svir River on its northern tributaries, the Uslanka and Vazhinka Rivers. Based on the research on the traditional medi-cine of the Russian and Finno-Ugric peoples, we managed to systematize the information obtained from the field journal, distinguishing two groups of healing practices: rational and irrational. According to the journal accounts, in the first half of the 20th c. (1931), among the rational treatment methods of the Trans-Onega people one can iden-tify phytotherapy (plant and herb substances); treatment with substances of animal origin, human excreta, mineral substances, food, precious metals, and combustion products; and physiotherapy, which should be considered as one of the most common methods of traditional medicine, along with phytotherapy. The natural environment (flora and fauna), methods of farming, as well as traditional crafts of the Trans-Onega people reflect in the medicine used. To the irrational methods of treatment one should ascribe the incantation tradition and sorcery. As the result of the conducted research, it was possible to identify similar features in the traditional medicine practices of the Ludian Karelians and Russians of the Trans-Onega region, induced by their long-term residence on the same territory and indicating close contacts between representatives of the two ethnic groups.

Distribution and accumulation of heavy metals in the catchment catenary complex and bottom sediments of the water body in an urban environment

Problem Statement. The pollutant intake into water bodies is one of the issues of urban areas. The pollutant input from the residential area of the city with surface runoff can many times exceed the input of pollutants from industrial and recreational areas, as well as from point pollutant sources. The objective of the study is 1) assess the distribution of chemical elements in catchments of water bodies in an urbanised area, 2) accumulation of chemical elements in solid matter of bottom sediments of surface water bodies with catchments in the functional areas of the city, 3) identify the main mechanisms of transfer of elements in solid matter from catchments to water bodies. The study was conducted on the example of Ekaterinburg (Russia). The material for the study consisted of the results of a landscape environmental and geochemical survey carried out in the city area in 2019–2021. Results. The distributions of element concentrations between functional landscape zones in the catenaries of the Kharitonovsky Garden and Chemodanchik reservoir catchments in Ekaterinburg were obtained. The distributions of metal concentrations in the catchment components show a tendency for the transfer of substance of different origin from the landscape components by lateral migration flows to the local basis of denudation (a drainless surface water body). At the same time in the bottom sediments of the reservoir there is a multiple accumulation of both pollutants and elements of typomorphic association. Dust fractions are the main carrier of contaminants in catchments.

Water quality of The Gambia River: A prospective drinking water supply

Drinking water in The Gambia is mostly derived from boreholes that could potentially be contaminated. The Gambia River, a major river in West Africa that covers 12 % of the country's area, could be more exploited for drinking water supply. During the dry season, the total dissolved solids (TDS), ranging from 0.02 to 33 g/L in The Gambia River, decreases with the distance to the river mouth with no major inorganic contamination. The freshwater (<0.8 g/L TDS) starts from Jasobo at approximately 120 km from the river mouth and extends by about 350 km to the eastern border of The Gambia. With a dissolved organic carbon (DOC) ranging from 2 to 15 mgC/L, the natural organic matter (NOM) of The Gambia River was characterised by 40–60 % humic substances of paedogenic origin. With such characteristics, unknown disinfection by-products could be formed if chemical disinfection, such as chlorination, was implemented during treatment. Out of 103 types of micropollutants, 21 were detected (4 pesticides, 10 pharmaceuticals, 7 per- and polyfluoroalkyl substances (PFAS)) with concentrations ranging from 0.1 to 1500 ng/L. Pesticides, bisphenol A and PFAS concentrations were below the stricter EU guidelines set for drinking water. These were mainly confined to the urban area of high population density near the river mouth, while the quality of the freshwater region of low population density was surprisingly pristine. These results indicate that The Gambia River, especially in its upper regions, would be well suited as a drinking water supply when using decentralised ultrafiltration treatment for the removal of turbidity, as well as, depending on pore size, to a certain extent microorganisms and DOC.

Analysis of the range of rectal drugs used for the treatment of proctologic diseases in Ukraine

Proctological diseases (acute and chronic hemorrhoids, anal fissure, proctitis) have frequent relapses, are characterized by a severe course and are common among broad strata of the working-age population. The leading clinical sign of this group of diseases is lesions of the mucous membrane of the rectum of varying degrees of severity which is accompanied by signs of inflammation, pain syndrome, deterioration of local circulation, involvement of secondary infection. It determines the use of complex products that combine anti-inflammatory, analgesic effects and promote the reparative processes of the mucous membrane. On the Ukrainian pharmaceutical market the number of such products is limited, most of them are foreign-made.&#x0D; The purpose of the work is to study the assortment of medicines for rectal use on the pharmaceutical market of Ukraine, to determine the feasibility and prospects of developing new combined soft medicines of domestic production. Methods of marketing, statistical and graphic analysis were used in the work from official sources of information: (The State Register of Medicinal Products of Ukraine and the ATC classification system).&#x0D; While researching the assortment of medicines for rectal use it was established that there are 34 names of medicines for rectal use in various forms of release on the domestic market, of which suppositories ‒ 55.9%, ointments ‒ 35.3%, creams ‒ 8.8%; the share of foreign-made medicines is 61.7% and domestic ‒ 38.3%. Complex synthetic medicines occupy 49.9% of the assortment and complex medicines of natural origin ‒ only 2.95%.&#x0D; In the production of soft medicines for rectal use, hydrophobic (40%), emulsion (40%) and diphilic bases (20%) are more often used. Solid fat (78.9%) is more often used in the production of suppositories.&#x0D; Considering the limited range of medicines for rectal use based on medicinal plant raw materials, it is advisable to develop new combined soft medicinal products which will include biologically active substances of plant origin.

Hygienic assessment of new pesticides migration from soil into the lower atmospheric air layer

Annotation. A great danger to the environment and the health of the population is the release of chemical substances of technogenic origin, in particular plant protection products into the atmospheric air. The aim of the study was to evaluate the hazard of harmful inhalation impact on the human body of new pesticides: triazolone herbicide amicarbazone, tricetone herbicide bicyclopyrone, and carboxamide fungicide pydiflumetofen, during their migration from soil to the lower atmospheric air layer. Mathematical modeling of the process of evaporation of the studied pesticides from soil to the atmospheric air was carried out and comparison of the maximum achievable concentrations with the average lethal concentration during acute inhalation exposure and hygienic standards in the air environment. Experimental study of the behavior of amicarbazone, bicyclopyrone, and pydiflumetofen in the "soil - atmospheric air" system was carried out in laboratory conditions. The concentrations of the studied substances in the air were determined by the method of high - performance liquid chromatography. It was established that amicarbazone, bicyclopyrone, and pydiflumetofen are non - volatile under saturated vapor pressure and Henry's law constant, and low - risk by the coefficient of possible inhalation poisoning. Acute inhalation poisoning as a result of evaporation from the soil into the lower atmospheric air layer is unlikely. The maximum achievable concentrations of the studied pesticides that can form in the air as a result of evaporation from the soil are significantly lower than their hygienic standards in the working zone. The migration levels of the studied substances from soil to the lower atmospheric air layer depend on their initial concentrations and the type of soil: bicyclopyrone and pydiflumetofen migrate more intensively from sandy soil than from leached chernozem. Threshold concentrations in the soil according to the air – migration indicator of harmfulness: amicarbazone – 0.05 mg/kg, bicyclopyrone – 1.0 mg/kg and pydiflumetofen – 1.2 mg/kg are formed in the plow layer of soil when researched pesticides are introduced according to 1th, 20th and 20th maximum consumption rates. The “soil - atmospheric air” system is not a leading link to the migration of the studied pesticides in the environment. The obtained results were used in the substantiation of hygienic standard of amicarbazone, bicyclopyrone and pydiflumetofen in soil.

Cow Urine: A Potential Benefits and Uses in Agriculture

A cow has high socio-cultural values, plays significant role in rural Economy, represent cattle wealth and bio-diversity. Cow urine is the most effective substance of animal origin with innumerable therapeutic values. Various kinds of pathogens such as bacteria, fungi, viruses, nematodes, and Mycoplasma causing diseases remain a major threat to public health. Despite tremendous progress in human medicine. Many researches have been done and have shown cow urine use for treatment of skin diseases, stomach diseases, diabetes, etc. It is also useful in agriculture for preparation of vermicompost, bio-fertilizers and bio pesticides. It plays a significant role in production of vegetables and control of plant diseases. Researches on cow urine are summarized in this article. However, more well-planned researches are required to prove its qualities and benefits in Agricultural production as well as medicinal importance in Botswana. Public awareness is also required to share knowledge and promote the importance and wide applications of cow urine.

EU Chemical Plant Protection Products in 2023: Current State and Perspectives

Agrochemicals are synthetic or hemi-synthetic crop protection substances which are supervised by the EU Regulation EC 1107/2009, which give rise to plant protection products (PPP) with market authorizations. Most of these active substances of chemical origin were transferred from the previous Annex I of Directive 91/414/EEC to Part A of Regulation EU 540/2011, with newly approved active substances mainly being listed in Part B or E, while renewed substances were moved from Part A to Part B or E. In this study, approved agrochemicals from the early part of 2023 are organized into categories, families, functions, usages, treated crop categories, regulatory characteristics, and maximum residue limits (MRLs). Perspectives regarding their evolution are also described together with pending approvals.

Bio-Based Jet Fuel Production by Transesterification of Nettle Seeds

The use of petroleum-based fuels in air transport and the increase in oil prices over the years have increased fuel costs. Due to this increase, fuel manufacturers and airline companies have started to search for alternative fuels. Since aviation has an important place in the transportation sector, biomass has the greatest potential in the search for renewable energy sources. Biological substances of plant and animal origin and containing carbon compounds are energy sources, and the fuels produced from them are called biofuels. Biofuels are an important source of sustainable energy, which greatly reduces the greenhouse gas effect, improves weather conditions, reduces dependence on oil produced from fossil fuels, and is important for new markets. The nettle seed oil used in the current study was purchased from the local market and was obtained using the cold-pressing method at low temperatures. After the completion of the transesterification process, a two-phase mixture consisting of biofuel-glycerin was obtained, and the upper phase containing fatty acids was taken and transferred to a clean tube. After the final washing processes, bio jet fuel was obtained by adding chemicals at certain rates. The analysis of the obtained fuel was conducted at the Tubitak Marmara Research Centre. When the report was evaluated and compared with international standards, consistent results were obtained. It can be predicted that sustainable fuels can replace fossil fuels in the future.

Addressing Risks Derived From the Commodification of Substances of Human Origin: A European Proposal Applicable Worldwide.

In view of the public consultation recently launched by the World Health Organization on Regulatory Convergence of Cell and Gene Therapy Products and the Proposal for a Regulation on substances of human origin (SoHO) repealing the European Union Directives on Blood and on Tissues and Cells, an opportunity arises to define an ethical and transparent framework of collaboration between industry and authorities responsible for SoHO-derived products, comprising medicines, medical devices, transfusion, and transplantation. The commodification of SoHO-derived medicinal products and medical devices entails important risks to the sustainability of healthcare systems and threatens the equitable access of patients to innovative therapies. It may also jeopardize the principle of altruistic donation of SoHO that is required for the treatment and survival of thousands of patients every year. This article puts forward several proposals aimed at reconciling the ethical principles of voluntary and unpaid SoHO donation and the noncommercialization of the human body with obtaining a profit that allows business activities, while ensuring high quality, safety, and efficacy standards of tissues and cells for clinical use.

An Overview of Biotechnological Applications in Ayurveda: Amalgamation of Modern Techniques and Science

Abstract: Health trends are constantly evolving, and so are health systems. No health system is perfect, hence the needed complement. Therefore, Western medicine (WM) practitioners are looking for traditional medicine (TM) and vice versa, as WM has an edge in an emergency while TM has in chronic diseases. However, the purpose of both systems is similar, which is a relief, despite different philosophies and methodologies. Globally, acceptance of TM by the scientific community is limited due to the lack of scientific evidence of benefits and efficiency, despite its potential. Ayurveda is one kind of the oldest TM systems of the world, serving humankind without changing a little, despite changes in geography, environment, weather conditions, and soil composition, which result in new diseases. Hence, the challenge of good health is arising. Therefore, there is a need to carry out research that would apply in its own way and satisfy the tenets of biomedical research too. The best way of doing this is multidisciplinary research by involving technology and Ayurvedic experts. Biotechnology can offer techniques from diagnosis to treatment of disease through substances of natural origin, including plants, animals and minerals. The present review is merely an overview of the possibilities which may be applied in Ayurveda in the present scenario. Ayurveda is the “science of life, and biotechnology is like “the service provider.” Therefore, biotechnology has all those techniques to serve Ayurveda up to the maximum and humankind at large. Even it may give rise to a new approach by combining Ayurveda and biotechnology.