Subsistence Farming Research Articles

Targeted mutagenesis of the CYP79D1 gene via CRISPR/Cas9-mediated genome editing results in lower levels of cyanide in cassava.

Cassava is the world's most essential food root crop, generating calories to millions of Sub-Saharan African subsistence farmers. Cassava leaves and roots contain toxic quantities of the cyanogenic glycoside linamarin. Consumption of residual cyanogens results in cyanide poisoning due to conversion of the cyanogens to cyanide in the body. There is a need for acyanogenic cassava cultivars in order for it to become a consistently safe and acceptable food, and commercial crop. In recent years, the CRISPR/Cas system, has proven to be the most effective and successful genome editing tool for gene function studies and crop improvement. In this study, we performed targeted mutagenesis of the MeCYP79D1 gene in exon 3, using CRISPR/Cas9, via Agrobacterium-mediated transformation. The vector design resulted in knockout in cotyledon-stage somatic embryos regenerated under hygromycin selection. Eight plants were recovered and genotyped. DNA sequencing analysis revealed that the tested putative transgenic plants carried mutations within the MeCYP79D1 locus, with deletions and substitutions being reported upstream and downstream of the PAM sequence, respectively. The levels of linamarin and evolved cyanide present in the leaves of mecyp79d1 lines were reduced up to seven-fold. Nevertheless, the cassava linamarin and cyanide were not completely eliminated by the MeCYP79D1 knockout. Our results indicate that CRISPR/Cas9-mediated mutagenesis is as an alternative approach for development of cassava plants with lowered cyanide content.

Hospitalization due to fire-induced pollution in the Brazilian Amazon: A causal inference analysis with an assessment of policy trade-offs

Developing countries by relying on agricultural fires trade respiratory health for cheaper land preparation and greater food security of subsistence farmers. In the Brazilian Amazon, thousands of kilometres of vegetation are annually burned, releasing pollutants that impact the health of 24 million inhabitants. The paper seeks to fill two crucial informational gaps for policy planning, namely, the size of the impact on the most pollution-susceptible groups, i.e., children and the elderly, and the priority locations for health-oriented intervention on fires. A municipal-monthly panel covering ten years of the Amazonian territory was analysed by relying on exogenous and high-resolution wind direction variation to identify the effect of fires on pollution and hospitalizations. This is an unparalleled effort for applying to a large geographical area spanning five million km2 a highly refined identification strategy relying on hourly gridded wind and fire data. As the result, one extra standard deviation of “upwind” fires was estimated to increase asthma-related hospitalization of the elderly in 0.03 days/month, that is, 4 % of the bed-days commonly demanded by such kind of hospitalization, an effect whose size decayed with the distance between fires and hospitals. A policy assessment uncovered the trade-off between respiratory health of the elderly and nutritional health of fire-dependent subsistence farmers, presenting a priority map for tackling the issue with municipal-level interventions. The targeting of non-subsistence fires is advised, what could avoid 28 days of hospitalization per year. It is thus demonstrated that the trade-offs inherent to agricultural fires could be better balanced by evidence-based targeting of fire prevention policy.

Soil ingestion, a key determinant of exposure to environmental contaminants. The case study of chlordecone exposure in free-range pigs in the French West Indies.

Ingested soil may expose free-range animals to environmental pollutants. In pigs, soil ingestion is few described whereas their burrowing behaviour suggests that it could be high. Although highly productive pigs are generally reared indoor, free-range farming is increasing in view of ethical considerations for animal welfare and is a common practice for subsistence agriculture systems. The experiment lasted 8 weeks (2 for adaptation, 6 for measurements) with 24 growing pigs of Guadeloupean Creole (CR) or Large White (LW) breeds. Pigs were assigned to 3 outdoor treatments: high pasture HP (>60 days of regrowth), low pasture LP (35 days of regrowth), and sweet potato SP (sweet potato field). Titanium (soil marker) and chromium (faecal output marker) contents of faeces, vegetation and soil samples were used to estimate individual daily soil ingestions. The average, 10th and 90th percentiles were 440, 200 and 726 g of dry soil per 100 kg body weight, respectively, without significant differences between the 3 outdoor treatments or the 2 breeds but with a significant period (i.e. week of measurements) × treatment interaction (P < 0.001). In the French West Indies, animals may be exposed to chlordecone (CLD), a very persistent organochlorine insecticide. Simulations of CLD tissue contamination due to ingestion of contaminated soil were carried out and compared to the maximum residue limit. These results show that grazing management needs to be adapted to effectively limit soil ingestion by pigs and the impact of a contaminated environment on the sustainability of pig systems.

Energy efficient drying technologies for sweet potatoes: Operating and drying mechanism, quality-related attributes.

Sweet potatoes (SPs) are a versatile tuberous crop used as subsistence and cash crop in raw and processed forms. The major issue with SPs is post-harvest losses, which result in noticeable quality decline because of inappropriate handling, storage, delayed transit, and sales, as well as microbiological and enzymatic activity. Drying is an excellent strategy for managing short postharvest storage life, preserving nutrients, and maximizing long-term benefits. However, several parameters must be considered before drying SPs, such as relative humidity, temperature, drying duration, size, and shape. The current review looks at the factors influencing SPs' moisture loss, drying kinetics, diverse drying methods, pretreatments, operating conditions, and their efficacy in improving the drying process, functional, and nutritional qualities. An optimal drying process is required to preserve SPs to obtain concentrated nutrients and improve energy efficiency to be ecofriendly. Drying sweet potatoes using traditional methods such as sun or open-air drying was found to be a slow process that could result in a lower quality. Various advanced drying techniques, like vacuum, infrared, freeze drying, and pretreatments such as ultrasound and osmotic dehydration, have been developed and are successfully used globally. The best-fit thin-layer models (Hii, Page, two-term, logarithmic) utilized for drying SPs and appropriate modeling methods for optimizing drying procedures are also discussed.

Genome-associations of extended-spectrum ß-lactamase producing (ESBL) or AmpC producing E. coli in small and medium pig farms from Khon Kaen province, Thailand

Thailand is undergoing rapid intensification of livestock production where small subsistence farms and medium sized commercial farms coexist. In medium farms, antimicrobials are prescribed by a veterinarian, whereas in small farms antimicrobial use remains largely unsupervised. The impact of these differences as well as other farming practices on the emergence and composition of antimicrobial resistance genes (ARGs) remains largely unknown. We analyzed 363 genomes of extended-spectrum ß-lactamase producing (ESBL) and/or AmpC producing Escherichia coli recovered from humans and pigs at small and medium farms from the Khon Kaen province, Thailand. We tested for genome-wide associations to identify links between ARGs, host, and farm size. Pig isolates from small farms were associated with mcr and qnr genes conferring resistance to colistin and fluoroquinolones, respectively. In contrast, pig isolates from medium farms were associated with ARGs conferring resistance to drugs commonly used on medium farms (i.e., streptomycin). ESBL plasmids from small farms co-carried ARGs conferring resistance to critically important antimicrobials more frequently compared to plasmid from medium farms. Frequent ARG combinations included blaCTX-M-55 + qnrS1 (29.8% vs 17.5% in small and medium farms, respectively), blaCTX-M-55 + qnrS1 + mcr-3.19 (5% vs 0%), blaCTX-M-14 + qnrS1 (9.3% vs 6.2%), and blaCTX-M-14 + qnrS1 + mcr-1.1 (3.1% vs 0%). The co-location on plasmids of ARGs conferring resistance to critically important antimicrobials as defined by the World Health Organization is concerning, and actions to curb their spread are urgently needed. Legislation on limiting antimicrobial sales and initiatives to better inform farmers and veterinarians on appropriate antimicrobial usage and farm biosecurity could help reduce antimicrobial use on farms.

Large-scale population disappearances and cycling in the white-lipped peccary, a tropical forest mammal.

Many vertebrate species undergo population fluctuations that may be random or regularly cyclic in nature. Vertebrate population cycles in northern latitudes are driven by both endogenous and exogenous factors. Suggested causes of mysterious disappearances documented for populations of the Neotropical, herd-forming, white-lipped peccary (Tayassu pecari, henceforth "WLP") include large-scale movements, overhunting, extreme floods, or disease outbreaks. By analyzing 43 disappearance events across the Neotropics and 88 years of commercial and subsistence harvest data for the Amazon, we show that WLP disappearances are widespread and occur regularly and at large spatiotemporal scales throughout the species' range. We present evidence that the disappearances represent 7-12-year troughs in 20-30-year WLP population cycles occurring synchronously at regional and perhaps continent-wide spatial scales as large as 10,000-5 million km2. This may represent the first documented case of natural population cyclicity in a Neotropical mammal. Because WLP populations often increase dramatically prior to a disappearance, we posit that their population cycles result from over-compensatory, density-dependent mortality. Our data also suggest that the increase phase of a WLP cycle is partly dependent on recolonization from proximal, unfragmented and undisturbed forests. This highlights the importance of very large, continuous natural areas that enable source-sink population dynamics and ensure re-colonization and local population persistence in time and space.

Impacts and economic costs of climate change on Mexican agriculture

This study quantifies the impacts of climate change on yields and the corresponding economic losses in six relevant crops that account for 65% of the harvested area in Mexico and are highly relevant in terms of consumption and economic value. The results show that crop yields could decrease considerably during this century, especially in the rainfed management system. Under a high-emission scenario, large reductions in yields are expected by the end of this century for both rainfed and irrigated management systems of maize (42%, 31.4%), rice (51.4%, 41.3%), sorghum (41.1%, 36.6%), soybean (59.1%, 44.9%), wheat (23.3%, 20.0%), and rainfed sugarcane (11.7%). At the national level, the present value of losses in the selected crops amounts to $37,934 million dollars, which represents about twice the current total national agricultural production of Mexico. Rainfed agriculture represents about 69% of these losses and reductions in maize yields account for almost 70% of the total losses. States such as Veracruz, Sinaloa, Tamaulipas, and Jalisco represent half of the total economic losses. However, about 16% of the aggregated losses occur in states with high levels of poverty and subsistence farming like Chiapas, Oaxaca, and Guerrero. Climate change will significantly increase the risks that already vulnerable subsistence farmers’ face in the present. Although ambitious mitigation efforts can reduce the estimated impacts in most of the crops, residual damages are considerable, and the prompt implementation adaptation strategies is required.

Towards REDD+ implementation: Deforestation and forest degradation drivers, REDD+ financing, and readiness activities in participant countries

This study provides an assessment of dominant drivers of deforestation and forest degradation across 29 REDD+ participant countries and total funds disbursed from the Forest Carbon Partnership Facility to 15 participant countries since they signed their respective Readiness Preparation Proposals (R-PPs), as well as what they spent the grants on. This study aims to provide information for policymakers and donors on the need to invest in REDD+, as well as the need for participant countries to channel funds to key activities for effectively and efficiently implementing REDD+. The assessment is based on participant countries’ R-PPs and FCPF readiness fund progress reports, using a content analysis approach. The expansion of agriculture, which includes both commercial and subsistence agriculture, was the predominant driver of deforestation, while logging, such as industrial and small-scale exploitation, was the main driver of forest degradation in most participating countries. In addition, we observed that the 15 participant countries received a total of USD 702.36 million for REDD+ readiness preparation. However, most countries have received less than USD 10 million for their readiness-related activities. We observed a huge disparity regarding the funds received between countries from sub-Saharan Africa and their Asian and Latin American counterparts, particularly Indonesia and Peru, respectively. Furthermore, almost all countries have spent their funds on policy and strategy development, stakeholder and consultation events, and public awareness creation. Relatively small amounts have been spent on capacity building and training. Thus, international donors need to focus on strengthening institutional capacities and building effective forest governance structures in countries participating in REDD+, and they should also provide platforms to engage with participant countries to tackle the main deforestation and forest degradation drivers. We suggest further studies to identify high-performing countries that have received lower-than-average funding, consider their deforestation pressures, the size of forests, and the emission reduction potential.

A gender approach to time and food security: a case study of Egypt

Women’s subsistence contribution to household food security is undeniable. However, due to the dominance of capital bias in most labour economies, rural women in particular lack agency to improve capacity and access to resources and opportunities, and they need to be more productive. This knowledge gap is the subject of many studies on sustainable agricultural development and gender equity. Although the consensus necessitates policy interventions targeting the gender gap in agriculture resources, not enough research has been dedicated to quantifying the importance of women subsistence agricultural labour within the Egyptian national context. This article seeks to statistically analyse women’s role in subsistence agriculture, estimated by a national time-use survey conducted in 2018 in Egypt. The goal is to highlight their capacity to contribute to food and nutrition security and quantify the real impact of rural women’s subsistence agriculture labour on rural household food security versus that of rural men. This will contribute to the debate that investigating rural women in agriculture promotes rural community sustainability and highlights their capacity for agricultural production.

Cultural Learning Among Pastoralist Children

Cultural learning is a key feature of culture and our humanity. Although studies exist on children’s learning in subsistence farmers and hunter-gatherers, comparable cross-cultural studies have not been conducted for pastoralists. The purpose of this paper is to identify and describe patterns of cultural learning in pastoralists and to compare these patterns to what we know about cultural learning in hunter-gatherers. The study utilizes 13 cultures coded as pastoralists in eHRAF World Cultures. The search located 198 texts in ethnographies with precise information on how pastoral children learn. Overall, we found that children acquired most pastoral skills and knowledge in early childhood, children were most likely to learn from parents (vertical transmission) and non-parental adults (oblique transmission), a relationship between age and specific modes of transmission did not exist, various forms of teaching were the most frequently mentioned processes of learning, and the frequency of teaching did not vary by the child’s age. When hunter-gatherer’s and pastoralist’s patterns of cultural learning were compared several similarities emerged: most accounts of learning occurred in early childhood, children were most likely to learn from parents and non-parental adults, and various forms of teaching were the most frequently mentioned processes of learning. Several differences in cultural learning between the two groups were identified: pastoralist ethnographers were less likely than hunter-gatherer ethnographers to mention learning from peers and more likely to mention learning via local enhancement and stimulus enhancement.

Survey of rural and urban happiness in Indonesia during the corona crisis

The COVID-19 pandemic has drastically changed urban life, and it can be said that the time is at hand when cities and rural areas should promote symbiotic projects. These projects are diverse and include medical conditions, socioeconomic activities, working conditions, information technology, food conditions, culture as well as education. According to previous studies, medical conditions are excellent, but well-being of the mental health of people in developed countries is higher in rural areas than in urban areas. Meanwhile, developing countries tend to have higher levels of well-being in urban life, while rural areas have lower levels of well-being and mental health, because of a focus on lagging economic activities and vulnerability in medical care. Preliminary interviews in Bali, Indonesia, the author's study area, revealed no livelihood change in subsistence farming villages during the COVID-19 disaster indicating no effects by the pandemic. Meanwhile, urban residents faced difficulties obtaining food due to the government curfew and halt in economic activities. Most workers lost their jobs and suffered hardships in the tourism industry. With this situation, the conditions are slightly different from the previous studies in developing countries mentioned above. Previous studies did not reveal any mental health and well-being assessment for life in the rural areas of developing countries during the corona disaster. This study aimed to clarify the reality of urban and rural well-being during the Corona Disaster in a developing country, namely Bali. The hypothesis is that in Bali, Indonesia, a developing country, the level of well-being under the corona disaster is higher for rural residents than for urban residents. Six groups were surveyed with 71 questions from the survey items of previous studies including the World Happiness Report conducted worldwide, WHR2020, AHI and The Oxford Happiness Survey. Face Sheet, Mental Health, Anxiety, Happiness, Good things due to corona, and Corona infection control behaviors were included. The questionnaire was categorical to allow for a quantitative analysis and began in September 2021. I collected 280 samples from two villages, each in rural and urban areas of Bali, and analyzed the results with simple cross-tabulations and a difference of means, factor analysis, multiple regression analysis and structural analysis of covariance. The analyses revealed a tendency toward inward self-loneliness in the urban areas and outward anxiety about one's surroundings in the rural areas. Under the corona disaster, subjects in rural areas stayed optimistic about external stress, in contrast to those in urban areas, who became inwardly oriented and negative. This point does not imply that well-being is higher among rural people, but it suggests that they are more mentally stress-tolerant because they are more likely to positively view the situation. Although the hypothesis was not proven, life in rural areas, where people have optimistic feelings and are not lonely, can be considered relatively humane and mentally healthy. This may indicate that the level of well-being of people living in rural areas is higher than people living in urban areas. The results of this study differ from previous studies in which people in rural areas of developing countries had lower levels of happiness and mental health. However, this study provides new knowledge about the situation of the corona disaster in developing countries by surveying the well-being of both rural and urban residents.

HIV-related stigma experiences and coping strategies among pregnant women in rural Uganda: A qualitative descriptive study.

BackgroundHIV-related stigma is a global problem among HIV clients with far-reaching effects including increased rates of mother-to-child transmission of HIV. However, HIV-related stigma experiences and coping strategies have received little attention, especially among pregnant women in rural settings. We explored the HIV-related stigma experiences and coping strategies among pregnant women in rural northern Uganda.MethodsThis was a qualitative descriptive study conducted among HIV-positive pregnant women seeking care at Aboke Health Center IV, Kole district, northern Uganda. We conducted 12 in-depth interviews using a semi-structured interview guide. Data were analyzed using the inductive thematic approach of Braun and Clarke.ResultsThe age range of the 12 participants was 17 to 35 years while the average duration with HIV since diagnosis was five years. The majority of the participants were subsistence farmers who had attained a primary level of education. Social rejection and public ridicule were identified as HIV-related stigma experiences while ignoring, social support, and prayers were identified as HIV-related coping strategies among the study participants.ConclusionEnacted HIV-related stigma is common among pregnant women in rural northern Uganda. Healthcare providers should work closely with HIV-positive women and other stakeholders to identify and strengthen HIV-related stigma coping strategies among pregnant women in rural settings.

Survey of Fungal Foliar and Panicle Diseases in Smallholder Sorghum Cropping Systems in Different Agro-Ecologies of Lower Eastern Kenya

Sorghum is a staple food crop and plays a critical role in subsistence farming in Kenya due to its adaptability to marginal agro-ecological zones. However, fungal diseases are among the major biotic constraints of sorghum production, causing over 70% yield loss in susceptible cultivars. Information on the distribution and severity of fungal diseases is important to establish efficient and improved strategies for integrated disease management of sorghum fungal diseases. The aim of this study was to determine the prevalence, incidence, severity and spatial distribution of fungal diseases on sorghum across agro-ecological zones of lower eastern Kenya. A total of 384 smallholder farmers’ fields were surveyed, and in each field, 30 plants were assessed for prevalence and incidence of fungal diseases using a W-shaped pattern to cover the whole field. Sorghum anthracnose was the most prevalent disease (71%), followed by leaf blight (70.18%), rust (68.41%), smut (63.02%), sorghum mildew (55.33%), Alternaria leaf spot (48.39%) and rough leaf spot (46.02%). Disease prevalence, incidence and severity varied among the investigated agro-ecological zones. There was a significant difference (p ≤ 0.05) in fungal disease severity across the investigated agro-ecological zones. Spatially interpolated disease maps showed a high variation in the distribution of various sorghum fungal diseases across the investigated agro-ecological zones of lower eastern Kenya. Morpho-cultural identification revealed the association of Colletotrichum sublineola with anthracnose, Curvularia lunata and Bipolaris cynodontis with leaf blight, Puccinia purpurea with rust, Peronosclerospora sorghi with downy mildew, Alternaria alternata with Alternaria leaf spot, Ascochyta sorghi with rough leaf spot and Sporisorium sorghi with covered kernel smut symptoms. Information obtained in this study will be useful to update knowledge on sorghum fungal diseases and provide a basis for the development of strategies for management and control of the investigated diseases.

Bambara groundnut production, grain composition and nutritional value: opportunities for improvements

AbstractBambara groundnut (Vigna subterranea L. Verdc; BGN) is an important legume grown mainly by small-scale subsistence farmers in sub-Saharan Africa, in parts of Thailand and Indonesia. It has a high concentration of seed carbohydrate (55–70%), protein (17–25%), fat (1.4–12%) and dietary fibre (5.2–6.4%). A range of biotic and abiotic stresses together with socio-economic constraints affect its productivity, yield and quality. The changing climate and a growing world population are putting pressure on food production, as world food supply is heavily reliant on few crops. As such, there is a need to broaden crop usage and increase yield of minor crops such as BGN. Improvements in production can potentially be achieved by a combination of advanced phenotyping, genotyping, environmental characterization and overall management approaches. Breeding for advanced lines is complicated by overreliance on landraces. This review aims to provide the current status of BGN production, production constraints and approaches to overcome these, as well as its grain composition and nutritional value. It further discusses and elaborates on potentially available opportunities for overall improvement, so that BGN, like all neglected crops, can play a valuable role in world food security. Efforts should be intensified to improve the overall utilization of BGN and its constituents to make it an economically viable crop.

Prevalence of pneumonia and malnutrition among children in Jigawa state, Nigeria: a community-based clinical screening study

ObjectiveTo estimate the point prevalence of pneumonia and malnutrition and explore associations with household socioeconomic factors.DesignCommunity-based cross-sectional study conducted in January–June 2021 among a random sample of households across all...

The Contribution of Romanian Agriculture to Global Warming and the Impact of Climate Change on Vegetable Crops

Abstract Climate change represents the most serious threat to the environment, with negative effects on agricultural productivity, both in the vegetable and livestock sectors. The article proposes an analysis of the agricultural sector in Romania from the perspective of the contribution to global warming and the impact of climate change on the plant sector. Data from the National Institute of Statistics, the Ministry of Agriculture and Rural Development, the National Meteorological Agency, the European Union and the European Environment Agency were used for the analysis. The analyzed data show that Romania’s agriculture is not intensive in terms of greenhouse gas emissions, the type of emissions being related to the way of soil management within the agricultural holdings, the number of animals in the holdings and the use of biomass in agricultural practices. Given that Romania’s agriculture is dependent on climatic conditions, food security and the well-being of the population largely depends on how farmers, especially those with subsistence farms, will adapt to climate change. Reducing the risks and effects of global warming requires an integrated strategy that includes climate change adaptation measures, complemented by climate change mitigation measures.

Assessment of Groundnut and Sorghum Intercropping

Intercropping approach is to maximizing the use of limited farm lands as well as attaining food security to the subsistence farmers. A field experiment was conducted during two consecutive rainy seasons (2013/14 and 2014/2015) at Sheikan Locality, North Kordofan State, Sudan to investigate the effects of intercropping groundnut with two varieties of sorghum on land use efficiency. The experiment consisted of eleven treatments: three sole crop of groundnut, a local sorghum variety (Zinari) and an improved sorghum variety (Butana) and eight spatial arrangements of 1:1, 1:2, 2:1 and 2:2 rows of groundnut with each of the local or improved sorghum varieties. Treatments were arranged in a randomized complete block design with four replications. Land equivalent ratio (LER), competitive indices and economic analysis were used to evaluate the productivity. The results revealed that the intercropping affected most of the characteristics studied. The results revealed that all competitive ratios (CRs) were less than 1 in all intercropping treatments in both seasons indicating that, all intercropping patterns were advantageous over sole cropping The arrangement of GSV1 2:2 (two groundnut rows alternating with two rows of sorghum var. Butana rows) was the best pattern in terms of land use efficiency, with LERs of 1.46 and highest cash returns (6345.3 SDG). The study concluded that, the arrangement of GSV1 2:2 is the best combination for maximum use of land.

Hybrid livelihoods: Maize and agrarian transformation in Southeast Asia's uplands

Hybrid maize farming has boomed across upland Southeast Asia in the past three decades. Recent studies suggest that the boom has resulted in diverse outcomes across countries. In Thailand, Vietnam, and Laos, the introduction of hybrid maize has often initially been linked to rising incomes and living standards, with little loss or concentration of landholdings. In contrast, recent studies from Myanmar argue that exploitative trader-farmer credit relations are driving rapid agrarian differentiation and dispossession. We analyze patterns of agrarian change associated with hybrid maize farming in southern Shan State, Myanmar, using data from a representative survey of 1562 rural households. Widespread farmer engagement in maize cultivation has contributed to deepening petty commodity production and some economic differentiation, but with little dispossession or negative impacts on food security. Credit provision by traders may benefit some larger farmers but does not actively penalize smaller producers. Hybrid maize is a ‘moderate risk, moderate reward’ crop that households integrate strategically into a broader patchwork of subsistence farming, higher risk cash crop production, and a growing mix of off-farm activities. Employment in non-farm enterprises, salaried work, and migration have significant potential to modify trajectories of differentiation associated with agriculture.

Participatory approaches for raising awareness among subsistence farmers in Tanzania about the spread of insecticide resistance in malaria vectors and the possible link to improper agricultural pesticide use

BackgroundInsecticide resistance is a key barrier to long-term malaria control, and it may be exacerbated by poor agricultural pesticide use. Current practices, however, do not link public health and agricultural pesticide use. This study investigated the perspectives of farmers and other stakeholders regarding the integration of agricultural and public health measures to address resistance. Additionally, the feasibility of participatory workshops to increase the farmers’ understanding and participation in pesticide stewardship was assessed.MethodsFour themes were investigated: pesticide awareness, practices, and opinions of; insecticide resistance in malaria vectors; the effectiveness of current malaria prevention tools; and the links between agricultural and public health pesticide usage. Participatory workshops and field training were held with entomologists, farmers, and agricultural specialists, focusing on agro-ecosystem practices related to pest control; and local farmers were involved in live-testing for insecticides resistance of local Anopheles mosquitoes.ResultsMost farmers (94%) considered pesticides effective, and nearly half of them (n = 198, 46.4%) could identify and name crop pests and diseases, mostly using local names. Three quarters were unaware of mosquito larvae in their fields, and only 7% considered their fields as potential sources of mosquitoes. Two thirds were uninformed of any effects that agricultural pesticides may have on mosquitoes, and three quarters had never heard of resistance in malaria mosquitoes. Experts from various sectors acknowledged that agricultural pesticides might impact malaria control through increasing resistance. They did, however, emphasize the importance of crop protection and advocated for the use of pesticides sparingly and non-chemical approaches. Farmers learnt how to discriminate between malaria vectors and non-vectors, identify agricultural pests and diseases, choose and use pesticides effectively, and conduct resistance tests during the participatory workshops.ConclusionThis study emphasizes the significance of enhancing subsistence farmers’ awareness of mosquito ecology as well as merging public health and agricultural pest management measures. Participatory techniques have the potential to raise stakeholder awareness and engagement, resulting in more effective resistance management.

Maternal carryover, winter severity, and brown bear abundance relate to elk demographics.

Ungulates are key components of ecosystems due to their effects on lower trophic levels, role as prey, and value for recreational and subsistence harvests. Understanding factors that drive ungulate population dynamics can inform protection of important habitat and successful management of populations. To ascertain correlates of ungulate population dynamics, we evaluated the effects of five non-exclusive hypotheses on ungulate abundance and recruitment: winter severity, spring nutritional limitation (spring bottleneck), summer-autumn maternal condition carryover, predation, and timber harvest. We used weather, reconstructed brown bear (Ursus arctos) abundance, and timber harvest data to estimate support for these hypotheses on early calf recruitment (calves per 100 adult females in July–August) and population counts of Roosevelt elk (Cervus canadensis roosevelti) on Afognak and Raspberry islands, Alaska, USA, 1958–2020. Increasing winter temperatures positively affected elk abundance, supporting the winter severity hypothesis, while a later first fall freeze had a positive effect on elk recruitment, supporting the maternal carry-over hypothesis. Increased brown bear abundance was negatively associated with elk recruitment, supporting the predation hypothesis. Recruitment was unaffected by spring climate conditions or timber harvest. Severe winter weather likely increased elk energy deficits, reducing elk survival and subsequent abundance in the following year. Colder and shorter falls likely reduced late-season forage, resulting in poor maternal condition which limited elk recruitment more than winter severity or late-winter nutritional bottlenecks. Our results additionally demonstrated potential negative effects of brown bears on elk recruitment. The apparent long-term decline in elk recruitment did not result in a decline of abundance, which suggests that less severe winters may increase elk survival and counteract the potential effects of predation on elk abundance.