The exploitation of coal resources has caused problems such as ground deformation, affecting the ecological environment. Spatiotemporal varying characteristics between land subsidence and ecological environmental quality (EEQ) are an important research hotspot. Using the SBAS-InSAR method, 64 Sentinel-1 images were utilized to monitor land subsidence in the Shenfu mining area, one of China’s largest coal source regions. And the remote sensing ecological index (RSEI) was used to monitor and evaluate EEQ of the Shenfu mining area. Global and local spatial autocorrelation methods were used to assess the spatial aggregation degree and change patterns over time. Spatial Econometric Models were employed to explore the impacts of land subsidence on EEQ. The results showed the following: (1) The average RSEI values in the Shenfu mining area were 0.531, 0.488, and 0.523 in 2016, 2018, and 2020, respectively; there was a slight downward trend in EEQ. The permanent scatter (PS) point deformation rate ranged from −353.40 mm/year to +246.24 mm/year, with average deformation rates of 0.1642, 0.2181, and 0.2490 mm/year, respectively. (2) There was a significant correlation and spatial agglomeration effect between land surface subsidence and EEQ. Low–high, high–low, and low–low clusters were the main types of relationships, indicating that land subsidence primarily has a negative spatial impact on the ecological environment. (3) The relationship between land subsidence and EEQ varied spatially in the Shenfu mining area at 500 × 500 grid units. This research can provide scientific guidance for disaster prevention and sustainable development in mining areas by considering long-term differences in ecological environmental quality and its correlation with land subsidence.