Subsequent Waves Research Articles

Testing exploratory narrative processing as a mechanism of change in identity status processes over 4 years in college-going emerging adults.

We examined the critical task of emerging adulthood-identity development-via analyses of trajectories of identity exploration and commitment over the college years, as well as whether narrative processing of important events during this period served as a mechanism of identity exploration and commitment. We took advantage of a unique and comprehensive longitudinal design, which included 12 waves of data, both quantitative and qualitative assessments, collected over 4 years, on two distinct college campuses in the Northwestern and Northeastern regions of the United States (Wave 1, n = 639; growth models using all waves, n = 251). Analyses for this study were preregistered after data collection was complete. We first examined trajectories of exploration and commitment via the dual-cycle identity model. Second, we examined whether exploratory processing in the narration of future self-defining memories at specific waves predicted changes in exploration and commitment in subsequent waves. Findings indicated that exploration and commitment showed trajectories typically viewed as normative (e.g., increasing adaptive forms of exploration and commitment), although trajectories for those at higher socioeconomic statuses differed by showing more exploration and less commitment. We failed to find evidence that exploratory processing predicted changes in exploration and commitment. Implications include distinctions in measurement and theoretical approaches to the study of identity development, the need for greater understanding of what is developing before theorizing how it develops, and the limitations of what is considered normative without attention to structural constraints, such as social class. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Conformational Heterogeneity of β-Barrel Membrane Proteins Observed In Situ Using Orthogonal Spin Labels and Pulsed ESR Spectroscopy.

Outer membrane proteins (OMPs) of Gram-negative bacteria are involved in many essential functions of the cell. They are tightly packed in the outer membrane, which is an asymmetric lipid bilayer. Electron spin resonance (ESR) spectroscopic techniques combined with site-directed spin labeling (SDSL) enable observation of structure and conformational dynamics of these proteins directly in their native environments. Here we depict a protocol for site-directed spin labeling of β-barrel membrane proteins in isolated outer membranes and intact E. coli using nitroxide, triarylmethyl (trityl), and Gd3+-based spin tags. Furthermore, subsequent continuous wave (CW) and orthogonal pulsed electron-electron double resonance (PELDOR) measurements are described along with experimental setup at Q-band (34GHz), the data analysis, and interpretation.

Pathology and molecular detection of influenza Asubtype H9N2 virus in commercial poultry in Nigeria, 2024

Background: After the first Avian Influenza H5N1 outbreak in Nigerian poultry in 2006, subsequent waves of outbreaks occurred, causing substantial losses. Despite effective control measures by 2008, a resurgence in 2015 led to further losses and required depopulation efforts. Aim: The aim of this study was to do Pathology and molecular detection of influenza A subtype H9N2 virus in commercial poultry in Nigeria during 2024. Methods: In February 2024, a poultry farmer reported high mortality in his mixed commercial poultry flock in Ibadan, Nigeria, submitting carcasses to the University of Ibadan's V.T.H. and the FAO Regional Laboratory at NVRI, Vom. Results: Necropsy of nine Isa Brown layers and three Abor Acre broilers revealed cyanosis of comb and wattles, generalized petechial and ecchymotic haemorrhages including shank haemorrhages with sinusitis, pneumonia, and severe greenish faecal pasting also observed. At histopathology, denudation of the tracheal epithelia and parabronchial epithelial necrosis, obliteration, with airsac oedema and emphysema were observed. At NVRI, qPCR detected an Influenza A H9N2 virus in several pooled organ samples of layers, and broilers and eliminating the avian infectious bronchitis and Newcastle disease viruses. Conclusion: This is the first report of H9N2 outbreak in commercial poultry in Southern Nigeria. The high pathogenicity shown in commercial poultry in this outbreak and the risk of dispersal of infected live poultry in Nigeria as previously seen in H5N1 require stakeholders’ intervention.

Review of Everyday Modernism: Architecture & Society in Singapore

Following Singapore’s transition to self-governance in 1959 and eventual independence from British rule as a republic in 1965, growing housing needs and subsequent waves of industrialization saw the island-nation’s landscapes and coastlines radically transformed, and its population’s ways of living and working fundamentally reorganized. Everyday Modernism: Architecture and Society in Singapore makes a case for what Chang and Zhuang term the “Singapore vernacular” covering this period up to the nation’s economic prosperity in the 1990s. Putting together an insightful volume that catalogues buildings and structures representative of the time that they were constructed, the authors evoke a vernacular that refers not so much to a single style or building typology, but everyday rather than iconic architecture, at all scales, built for civic, industrial, educational, and entertainment purposes. The authors understand the everyday-ness of this architectural modernism as a concept that is “both specific and general,” which describes buildings of unknown authorship, such as those planned by architects working for government agencies, as opposed to the “heroic modernism” of contemporary projects designed by renowned local practitioners. Another facet of the everyday is the expanded sense of architecture that describes not only building projects but utilitarian structures—overhead bridges, multistorey carparks, hawker centers that house affordably priced food stalls—interwoven in the modern city’s infrastructure.

Fracture Mechanics Induced by Drilling and Blasting in Underground Openings: Prevailing Practices and Studies

This article presents the prevailing practices and literature review on the study of fracture mechanics of rock mass, during the construction of underground openings, by drill and blast method (DBM). The paper makes an introductory discussion on basic fracture and failure mechanics on the rock, followed by a review due to the blast-induced failure mechanism. The damage zone in drill and blast openings is discussed which is followed by the conclusions of the literature. DBM is a widely acceptable and broadly applicable approach in the underground construction method. The blasting damaged zones are classified as the borehole expansion zone, crushed zone, nonlinear fracture zone, and radial crack propagation zone. Blasting activities create stress waves in construction work, which fundamentally influence the fracture and failure mechanics of rock with pre-existing discontinuities, in-situ stress, and groundwater conditions. On the other hand, the cumulative action of subsequent explosion gas and stress waves defines the final damage limit and crack pattern map. Thus, it is of utmost importance to investigate the blast-induced fracture and failure mechanism to mitigate the instability in the underground openings.

Mental Health of Audiologists and Speech Language Pathologists in India during COVID-19: A Survey

Introduction The World Health Organization (WHO) declared coronavirus disease-2019 (COVID-19), the most current coronavirus epidemic, a global pandemic on March 11, 2020. Healthcare personnel were significantly impacted by social isolation regulations and the pandemic. The purpose of the present study was to assess the impact of the COVID-19 pandemic on speech-language pathologists’ and audiologists’ mental health in India. Methods In order to meet the objectives of the current study, the General Health Questionnaire (GHQ-12) was utilized as a screening tool for psychological problems faced by Indian audiologists and speech-language pathologists. The poll gathered replies from 153 audiologists and speech-language pathologists working in India. Results The results of the current study showed that 51.80% of audiologists and speech-language pathologists experienced stress. Between 10 and 15% of audiologists and speech-language pathologists, have trouble focusing, making decisions, and dealing with challenges. Fifteen to twenty-five percent (15-25%) of professionals struggle to contribute meaningfully beyond obstacles are unable to engage in daily activities, lose confidence, and feel unworthy. Conclusion According to the findings of the current study, 30-50% of audiologists and speech-language pathologists in India experienced poor mental health during the COVID-19 outbreak. The current study showed how crucial it was to provide psychological assistance and treatment for the workforce of audiologists and speech-language pathologists throughout subsequent COVID-19 pandemic waves.

Intraepithelial Inclusions on Urinalysis Screening among COVID-19 Cases: Are they Covicytes?-A Hospital-Based Cohort Study with Narrative Review.

"Intraepithelial inclusions on urinalysis screening among COVID-19 cases: Are they Covicytes?-A hospital-based cohort study with narrative review." Coronavirus disease 2019 (COVID-19)-associated delayed acute kidney injury (AKI) is often reported in subsequent waves of the pandemic. Early intervention and regular follow-up influence the outcome and inhibit progression into chronic kidney disease (CKD). This is the first study to identify urinary cytomorphological abnormalities (Covicytes) and predict COVID-19-associated delayed AKI with a narrative review of the possible etiologies for intraepithelial inclusions. A hospital-based cohort study with a narrative review. Screening urinalysis to assess the cytomorphology of epithelial cells (ECs) and inclusions in Leishman and periodic acid-Schiff (PAS)-stained smears by two independent pathologists was performed in reverse transcriptase polymerase chain reaction (RT-PCR)-confirmed COVID-19 cases at a tertiary care center. Basic statistical tools were used for descriptive statistical analysis, and data were expressed in mean, proportion, and frequency. Cytomorphological abnormalities (48/188) were predominant among adult males. Leukocyturia (39/48) with positive nitrite test (28/39), high ECs (27/48) and squamous cell-to-tubular EC (SC:TEC) ratio, intraepithelial intracytoplasmic inclusions predominantly in TECs (Covicytes), and multiple well-visualized, perinuclear PAS-negative neutrophilic vacuoles (17/39) were found. The association with preexisting diabetes (31/48), hypertension (10/48), and disease severity was noted. This study reported COVID-19-associated urinary cytomorphological abnormalities and interesting unique inclusions (Covicytes) that may be a result of underlying inflammatory changes, reactive hyperplasia, degenerative changes, or defective endocytosed vacuoles. The possible etiologies for renal inclusions were reviewed. We recommend compulsory baseline and follow-up urinary cytology screening for all COVID-19-suspected patients to detect and predict delayed AKI before clinical and biochemical manifestation during disease endemicity.

Single-shot optical imaging with spectrum circuit bridging timescales in high-speed photography.

Single-shot optical imaging based on ultrashort lasers has revealed nonrepetitive processes in subnanosecond timescales beyond the recording range of conventional high-speed cameras. However, nanosecond photography without sacrificing short exposure time and image quality is still missing because of the gap in recordable timescales between ultrafast optical imaging and high-speed electronic cameras. Here, we demonstrate nanosecond photography and ultrawide time-range high-speed photography using a spectrum circuit that produces interval-tunable pulse trains while keeping short pulse durations. We capture a shock wave propagating through a biological cell with a 1.5-ns frame interval and 44-ps exposure time while suppressing image blur. Furthermore, we observe femtosecond laser processing over multiple timescales (25-ps, 2.0-ns, and 1-ms frame intervals), showing that the plasma generated at the picosecond timescale affects subsequent shock wave formation at the nanosecond timescale. Our technique contributes to accumulating data of various fast processes for analysis and to analyzing multi-timescale phenomena as a series of physical processes.

States of Emergency, Simultaneous Overreach and Underreach and the COVID-19 Pan(dem)ic

AbstractPrevious research has neglected how repeated declarations of states of emergency (SsoE) in response to the same emergency may combine with executive overreach and underreach within a single jurisdiction, undermining the authority of the SsoE as a legal institution and increasing the vulnerability of the constitutional system as a result. This article examines how decision-makers’ commitment to a culture of justification is central to avoiding emergency mismanagement via underreach, overreach or their combination. The simultaneous instances of executive overreach and underreach as emergency management failures are studied via the Slovak case, which was celebrated for its initial response to the COVID-19 pandemic but castigated for its failure to contain the subsequent waves. The analysis of the legal framework of the SsoE and the justifications for SsoE declarations uncovers the lack of justifications for the patterns of simultaneous executive underreach and overreach, underscoring the elusiveness of these categories. The limited justifications for the decisions demonstrated by the “government in panic” point to the undermining of the SsoE as a legal institution. The article concludes with highlighting how leaders’ role conceptions as democratic emergency managers might be necessary to sustain the authority of the SsoE.

Impact of Surge Strain and Pandemic Progression on Prognostication by an Established COVID-19–Specific Severity Score

IMPORTANCE: Many U.S. State crisis standards of care (CSC) guidelines incorporated Sequential Organ Failure Assessment (SOFA), a sepsis-related severity score, in pandemic triage algorithms. However, SOFA performed poorly in COVID-19. Although disease-specific scores may perform better, their prognostic utility over time and in overcrowded care settings remains unclear. OBJECTIVES: We evaluated prognostication by the modified 4C (m4C) score, a COVID-19–specific prognosticator that demonstrated good predictive capacity early in the pandemic, as a potential tool to standardize triage across time and hospital-surge environments. DESIGN: Retrospective observational cohort study. SETTING: Two hundred eighty-one U.S. hospitals in an administrative healthcare dataset. PARTICIPANTS: A total of 298,379 hospitalized adults with COVID-19 were identified from March 1, 2020, to January 31, 2022. m4C scores were calculated from admission diagnosis codes, vital signs, and laboratory values. MAIN OUTCOMES AND MEASURES: Hospital-surge index, a severity-weighted measure of COVID-19 caseload, was calculated for each hospital-month. Discrimination of in-hospital mortality by m4C and surge index-adjusted models was measured by area under the receiver operating characteristic curves (AUC). Calibration was assessed by training models on early pandemic waves and measuring fit (deviation from bisector) in subsequent waves. RESULTS: From March 2020 to January 2022, 298,379 adults with COVID-19 were admitted across 281 U.S. hospitals. m4C adequately discriminated mortality in wave 1 (AUC 0.779 [95% CI, 0.769–0.789]); discrimination was lower in subsequent waves (wave 2: 0.772 [95% CI, 0.765–0.779]; wave 3: 0.746 [95% CI, 0.743–0.750]; delta: 0.707 [95% CI, 0.702–0.712]; omicron: 0.729 [95% CI, 0.721–0.738]). m4C demonstrated reduced calibration in contemporaneous waves that persisted despite periodic recalibration. Performance characteristics were similar with and without adjustment for surge. CONCLUSIONS AND RELEVANCE: Mortality prediction by the m4C score remained robust to surge strain, making it attractive for when triage is most needed. However, score performance has deteriorated in recent waves. CSC guidelines relying on defined prognosticators, especially for dynamic disease processes like COVID-19, warrant frequent reappraisal to ensure appropriate resource allocation.

A new wave of migration in Ukraine on the background of Russian invasion: dynamics, challenges and risks

The large-scale Russian military invasion of Ukraine in February 2022 led to a significant increase in the indicators of migration flows of the Ukrainian population, the absolute values of which increased by 10 or more times compared to previous periods. The authors evaluated the dynamics of indicators of population migration in the state, both internal and external, and found a number of similarities and differences between subsequent migration waves. Based on the study, the indicators of migration flows were grouped into three waves disaggregated by time. The first group is from the obtaining the country's independence to the first stage of the military invasion of Ukraine, as a period of peace in the state (1991–2014). The second one begins with Russian aggression (2014–2022) and the third group is a large-scale invasion of the Russian army into Ukraine (from 2022). The use of statistical and economic methods (scientific abstraction, analysis and synthesis, descriptive statistics’ methods, econometric modelling, regression point and interval estimates) and SWOT analysis provided a basis for determining a number of challenges, threats and opportunities for the countries of origin and destination regarding the prospects for the development of migration processes.

An integrated numerical model for landslide-generated waves: a case study of the Gongjiafang landslide, Three Gorges Reservoir, China

ABSTRACT Landslide is a frequent geological hazard in the Three Gorges reservoir area of China. Impulse waves could be created and cause substantial damage if the landslide collides with a water body. A nonlinear shallow water equation in combination with a progressive landslide model is proposed to simulate this catastrophic disaster. The proposed approach is distinguished by rapid calculation, less computational burden, and high practical value. By simulating the 2008 Three Gorges Reservoir Gongjiafang landslide and the subsequent impulse waves, we illustrated and validated this methodology. The initiation and motion processes of the landslide, as well as the generation, propagation, and run-up processes of impulse waves, were thoroughly performed. The simulation results were in good agreement with the field survey data, demonstrating the viability of the proposed approach for simulating the impulse waves triggered by landslide events. This numerical approach is beneficial for engineers to perform a preliminary estimation of landslide-generated waves and further facilitate the prevention and mitigation of geological hazards.

459. Characteristics and Presentations of Hospitalized Children due to three predominate COVID-19 variants within a healthcare network

Abstract Background Variants of SARS-CoV-2 caused three major waves of infections in NJ. Children and young adults were hospitalized with varying symptoms and severity of illness depending on the variant. The objectives of this study are to describe differences in the demographic and clinical characteristics, severity of illness and outcomes in pediatric patients during the different SARS-CoV-2 waves in our hospital network. Methods We conducted a retrospective study of pediatric patients admitted to one of our three network hospitals with SARS-CoV-2 infection or with post-COVID-19 inflammatory conditions such as Multisystem Inflammatory Syndrome in Children (MIS-C) during the different waves of infection. Patients who were asymptomatic but incidentally tested positive for SARS-CoV-2 and were hospitalized for unrelated indications were excluded. We defined the timelines as follows: Wave 1 occurred between March 1, 2020 and May 31, 2020; wave 2 occurred between November 1, 2020-January 31, 2021; and wave 3 occurred between December 1, 2021-February 28, 2022. Demographic characteristics, clinical features, and outcomes were recorded and compared with respect to the different variants. Results Of 351 total patients included in this study, 74 were admitted during wave 1, 94 during wave 2, and 181 during wave 3. The median age of patients decreased from wave 1 (11.5 years) to wave 3 (3 years) (p=0.0034). 87.7% of the patients were unvaccinated. The overall incidence of admissions related to COVID-19 pneumonia decreased in wave 3 compared to wave 1 and 2. COVID-19 bronchiolitis or croup admissions occurred mostly in wave 3. No significant difference in intensive care admissions was observed in any wave. Length of stay decreased across the waves (p< 0.0001). Treatments required did not vary between the waves except for a decrease in antibiotic use with each subsequent wave (p< 0.0001). Conclusion The impact of COVID-19 on the pediatric population differs from the adult population, and the overall numbers of hospitalized children has mirrored the peak in cases observed during each infection wave. Our study illustrates the changes in clinical presentation and severity observed with the different coronavirus variants. Disclosures Tara Lozy, MA in Data Science, Eli Lilly & Company: Stocks/Bonds

Forward numerical investigation of potential tsunami deposits in the South China sea: A case study of Nan'ao Island

The South China Sea (SCS) coastlines are particularly vulnerable to tsunamis due to rapid urbanization and dense infrastructures. However, whether the SCS has experienced ocean-wide tsunamis is still under debate. Some geological records from the offshore islands inside the SCS imply a regional tsunami event may have occurred approximately 1000-yr-ago. We address these questions for the first time using forward numerical simulations in the SCS: what source could be responsible for such deposits, how the deposits were formed and at what conditions do the deposits could be preserved? Here, we assume the validity of the tsunami deposit hypothesis and use the forward model COMCOT-SED to investigate if these tsunami deposits can be linked to potential seismogenic tsunami sources inside the SCS, including megathrust earthquakes (Mw8.8–9.2) from the Manila subduction zone (MSZ) and the 1918 Mw7.5 Nan'ao earthquake from the Littoral Fault Zone (LFZ) in the northern continental shelf of the SCS. We also utilize a reported tsunami deposit from Qing'ao Embayment, Nan'ao Island, China, to exemplify the deposit formation process in a typical barrier-low coastal plain system. Quantitative analysis suggests large earthquakes from the MSZ may account for the reported geological deposits. The full-rupture earthquake scenario could best explain the depositions if such extreme case is geophysically and geologically plausible. If not, earthquakes (∼ Mw 9.0) covering the northern (16–23.5°N) or southern portion (14–19°N) of the MSZ could also be suitable candidates. Such great earthquakes can generate large tsunami waves (>7 m) that inundate the Qing'ao Embayment, transporting sediments and leaving tsunami deposits in low-lying areas. Conversely, tsunamis triggered by nearshore earthquakes in the LFZ cannot cause deposition in Qing'ao Embayment. The barrier-low coastal plain system is well-suited for preserving tsunami deposits, which remain unaltered by subsequent tsunami waves. Our findings offer a quantitative reference for paleo-tsunami research in the SCS and enhance global understanding of the tsunami deposition process in barrier-low coastal plain systems.

Seasonality of California Central Coast Microseisms

ABSTRACT Linear scattering of ocean wave energy at the ocean–continent transition structure causes the primary microseism at a period of 14 s. Subsequent nonlinear wave–wave interactions produce the secondary microseism signal at half the primary microseism period (Longuet-Higgins, 1950; Haubrich et al., 1963). We use three years (2018–2022) of seismic data from an ongoing microarray deployment in the UC Santa Barbara Sedgwick Reserve, situated in the Santa Ynez Valley, to constrain seasonal and long-term microseismic noise characteristics for this portion of California’s central coast. Ancillary buoy data (spectral data, wave height, wind speed and direction) from the National Oceanic and Atmospheric Administration are used to explore the causal relationship between ocean swell and the generation of microseisms. This region is found to exhibit strong seasonality in the primary and secondary microseism bands (0.05–0.1 and 0.1–0.3 Hz, respectively), with much higher noise levels in the winter compared with the summer, especially for the secondary microseism (15.4 dB). We also observe a systematic shift in the peak frequency of the secondary microseism between the winter (∼0.14 Hz) and summer (∼0.20 Hz) months, which may reflect a difference in sources of secondary microseisms between the two seasons. Local buoy wave height and spectral data are well correlated with seismic power spectra during times of incoming storm swell in winter, indicating locally generated microseisms along the central coast during this season.

Comparing magnetic pushing to underwater explosions for flyer acceleration

We present results exploring various methods of aluminum flyer acceleration. One method uses the shock wave generated by underwater electrical explosions of thin foils supplied by a pulse generator with stored energy of ∼4.7 kJ. Utilizing the shock created by an exploding foil, a maximal free flyer velocity of ∼2000 m/s is obtained. This acceleration method is compared to results exploiting only magnetic pushing to accelerate flyers using a common strip-line configuration, resulting in much lower velocities of ∼300 m/s. We also present a modified strip-line configuration, for which a significant increase in the flyer velocity to ∼1200 m/s is measured. Finally, a hybrid strip configuration, incorporating both the effects of magnetic pushing and acceleration by exploding foil and its subsequent shock wave, results in ∼1400 m/s flyer velocity. These experimental results are analyzed by numerical simulations and analytical modeling of the conservation equations of mass and momentum.

Sleep Disorders and Cognitive Aging Among Cognitively Impaired Versus Unimpaired Older Adults.

Sleep disorders often predict or co-occur with cognitive decline. Yet, little is known about how the relationship unfolds among older adults at risk for cognitive decline. To examine the associations of sleep disorders with cognitive decline in older adults with unimpaired cognition or impaired cognition (mild cognitive impairment and dementia). A total of 5,822 participants (Mage = 70) of the National Alzheimer's Coordinating Center database with unimpaired or impaired cognition were followed for 3 subsequent waves. Four types of clinician-diagnosed sleep disorders were reported: sleep apnea, hyposomnia/insomnia, REM sleep behavior disorder, or "other." Cognition over time was measured by the Montreal Cognitive Assessment (MoCA) or an estimate of general cognitive ability (GCA) derived from scores based on 12 neuropsychological tests. Growth curve models were estimated adjusting for covariates. In participants with impaired cognition, baseline sleep apnea was related to better baseline MoCA performance (b = 0.65, 95% confidence interval [95% CI] = [0.07, 1.23]) and less decline in GCA over time (b = 0.06, 95% CI = [0.001, 0.12]). Baseline insomnia was related to better baseline MoCA (b = 1.54, 95% CI = [0.88, 2.21]) and less decline in MoCA over time (b = 0.56, 95% CI = [0.20, 0.92]). Furthermore, having more sleep disorders (across the 4 types) at baseline predicted better baseline MoCA and GCA, and less decline in MoCA and GCA over time. These results were only found in those with impaired cognition and generally consistent when using self-reported symptoms of sleep apnea or insomnia. Participants with sleep disorder diagnoses may have better access to healthcare, which may help maintain cognition through improved sleep.

Phytoplankton blooms and fish kills in Lake Tanganyika related to upwelling and the limnological cycle

Characterizing the processes which cause upwelling in the East African Great Lakes enables prediction of the spatial–temporal variability of phytoplankton blooms and increases in fish abundance or incidence of fish kills. The influence of the southeast trade winds on the limnology of Lake Tanganyika is well known. Upwelling occurs to the south and downwelling to the north from May to September and subsequent upwelling and blooms at the northern end of the lake. However, the influence of the shifts in wind speed and direction after the SE trade winds, including the Northeast trade winds, and any further internal wave driven upwelling requires investigation. Here we use images from remote-sensing during a 12 year period (2003–2014) to illustrate intense phytoplankton blooms at the southeast of Lake Tanganyika in April-May, a period characterized by relaxation of the Northeast trade winds. Phytoplankton blooms progressed around the lake after a massive cyanobacteria bloom at the northern end in September 2018; recent and historical observations and local knowledge show similar patterns. Lake Tanganyika limnological cycle is revised to include four main periods: two trade winds seasons and two inter-monsoon seasons (April-May and September-October) with lake-wide up and downwelling of the thermocline associated with the monsoons, and upwelling associated with subsequent internal waves in the inter-monsoon period which are linked with increased phytoplankton blooms and sometimes also fish kills.

Outcomes comparison between the first and the subsequent SARS-CoV-2 waves - a systematic review and meta-analysis.

In the beginning of the SARS-CoV-2 pandemic, health care professionals dealing with COVID-19 had to rely exclusively on general supportive measures since specific treatments were unknown. The subsequent waves could be faced with new diagnostic and therapeutic tools (e.g., anti-viral medications and vaccines). We performed a meta-analysis and systematic review to compare clinical endpoints between the first and subsequent waves. Three databases were assessed. The primary outcome was in-hospital mortality. The secondary outcomes were intensive care unit (ICU) mortality, ICU length of stay (LOS), acute renal failure, extracorporeal membrane oxygenation (ECMO) implantation, mechanical ventilation time, hospital LOS, systemic thromboembolism, myocarditis and ventilator associated pneumonia. A total of 25 studies with 126,153 patients were included. There was no significant difference for the primary endpoint (OR=0.94, 95% CI 0.83-1.07, p=0.35). The first wave group presented higher rates of ICU LOS (SMD= 0.23, 95% CI 0.11-0.35, p<0.01), acute renal failure (OR=1.71, 95% CI 1.36-2.15, p<0.01) and ECMO implantation (OR=1.64, 95% CI 1.06-2.52, p=0.03). The other endpoints did not show significant differences. The analysis suggests that the first wave group, when compared with the subsequent waves group, presented higher rates of ICU LOS, acute renal failure and ECMO implantation, without significant difference in in-hospital or ICU mortality, mechanical ventilation time, hospital LOS, systemic thromboembolism, myocarditis or ventilator- associated pneumonia.

Astrocyte ensheathment of calyx-forming axons of the auditory brainstem precedes accelerated expression of myelin genes and myelination by oligodendrocytes.

Early postnatal brain development involves complex interactions among maturing neurons and glial cells that drive tissue organization. We previously analyzed gene expression in tissue from the mouse medial nucleus of the trapezoid body (MNTB) during the first postnatal week to study changes that surround rapid growth of the large calyx of Held (CH) nerve terminal. Here, we present genes that show significant changes in gene expression level during the second postnatal week, a developmental timeframe that brackets the onset of airborne sound stimulation and the early stages of myelination. Gene Ontology analysis revealed that many of these genes are related to the myelination process. Further investigation of these genes using a previously published cell type-specific bulk RNA-Seq data set in cortex and our own single-cell RNA-Seq data set in the MNTB revealed enrichment of these genes in the oligodendrocyte lineage (OL) cells. Combining the postnatal day (P)6-P14 microarray gene expression data with the previously published P0-P6 data provided fine temporal resolution to investigate the initiation and subsequent waves of gene expression related to OL cell maturation and the process of myelination. Many genes showed increasing expression levels between P2 and P6 in patterns that reflect OL cell maturation. Correspondingly, the first myelin proteins were detected by P4. Using a complementary, developmental series of electron microscopy 3D image volumes, we analyzed the temporal progression of axon wrapping and myelination in the MNTB. By employing a combination of established ultrastructural criteria to classify reconstructed early postnatal glial cells in the 3D volumes, we demonstrated for the first time that astrocytes within the mouse MNTB extensively wrap the axons of the growing CH terminal prior to OL cell wrapping and compaction of myelin. Our data revealed significant expression of several myelin genes and enrichment of multiple genes associated with lipid metabolism in astrocytes, which may subserve axon wrapping in addition to myelin formation. The transition from axon wrapping by astrocytes to OL cells occurs rapidly between P4 and P9 and identifies a potential new role of astrocytes in priming calyceal axons for subsequent myelination.