Subsequent Visualization Research Articles

МАСШТАБУВАННЯ ПРОЦЕСУ ЗМЕНШЕННЯ НЕГАТИВНОГО ВПЛИВУ АВІАЦІЙНОГО ШУМУ

The article proposes an approach to reduce the negative impact of aviation noise on the population by scaling the process, which includes the analysis of the system «airport infrastructure (AI) - external environment (AF)», calculating and digitizing the main characteristics of noise, and improving the implementation of the Balanced Approach concept. Object of study - parametric pollution of the aviation transport system, taking into account the development of the basic elements. The purpose of the article is to propose to build an effective algorithm for the necessary measures aimed at reducing the negative impact of aviation noise. The research method is a systematic analysis of the mutual influence of parametric pollution of the aviation transport system on the environment. The inextricable link between nature, society and transport, which has a mutual character, is determined. The threat of an increase in the number of people exposed to aviation noise necessitates the search for ways and methods of public consciousness based on fundamentally new world-views and values. The results of the article can be implemented in the implementation of the concept of «Balanced Approach» to the problems of aviation noise management, provided by the national legislation of Ukraine. Predictive assumptions about the development of the object of study - A rational solution is to develop an online system for gathering information on noise from aviation transport system (with geospatial information) with subsequent visualization (interactive map of Ukraine) with generalized data of levels and noise contours, from all airports for easy and clear access to information. KEYWORDS: AVIATION NOISE, SCALING UP, BALANCED APPROACH, AERODROME ENVIRONMENT, DIGITALIZATION, PARAMETRIC EMISSION, AVIATION TRANSPORT SYSTEM.

Social Bonds as an Instrument of Responsible Investment

Research dedicated to structuring the scientific sphere in social bonds as an instrument responsible investing. The purpose of the paper is to form an information and bibliographic field of research of social bonds as tools of VI and their statistical support in the context of a new type of SIP. It was found that social bonds as a public-private partnership aimed at achieving financial, social impact, and influence on the development fully meets the criteria of the VI instrument, but is studied primarily in the context of the empirical. The reason for this is the fact that the keys were based on practical use, and there is a large number of publications on a wide range of topics in the Scopus database without specifying the sound supply of the arms of 11,207 documents. A donation for the analysis of the informational-biometric field of social oblasts as for the tool BI will be received from the number of publications indexed by Scopus (Elsevier). Hours of progress for analysis at the end of 15 years (2005-2020 years). As for the research methodology, it is quite broad and includes built-in tools for analyzing the publication of the Scopus database, which provide a general idea of the information and bibliometric field of social bonds, software bibliometric analysis based on VOSviewer 1.6.15 for clustering analyzed publications with subsequent visualization, Google tools Trends, and Google Data to study Internet search activity information and statistics for this tool. The first two methods allowed to analyze the publishing activity on the studied tools in academic circles on the leading base of scientific publications. The third is to describe the current trends in the development of information and statistical support of social bonds. The application of these methods for structuring the scientific sphere of social bonds allowed to justify its status – as one that is formed and requires additional information and statistical support. Keywords: social bonds, responsible investing, Scopus (Elsevier), VOSviewer.

Preparation of Cultured Cells Using High-Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope.

Transmission electron microscopy (TEM) is an invaluable technique used for imaging the ultrastructure of samples, and it is particularly useful when determining virus-host interactions at a cellular level. The environment inside a TEM is not favorable for biological material (high vacuum and high energy electrons). Also biological samples have little or no intrinsic electron contrast and rarely do they naturally exist in very thin sheets, as is required for optimum resolution in the TEM. To prepare these samples for imaging in the TEM therefore requires extensive processing which can alter the ultrastructure of the material. Here we describe a method which aims to minimize preparation artifacts by freezing the samples at high pressure to instantaneously preserve ultrastructural detail, then rapidly substituting the ice with resin to provide a firm matrix which can be cut into thin sections for imaging. Thicker sections of this material can also be imaged and reconstructed into 3D volumes using electron tomography.

Development of a data collection system for a CNC system using cloud FRED technology and OPC UA specification

Collecting data from process equipment is currently an interesting task. The use of complex SCADA systems is not always possible and is usually an expensive solution. For some tasks, in-depth analysis of the received technological information is not needed, but visualization of the necessary data is sufficient. In control systems, it is usually not possible to create custom HMI screens. The use of new approaches based on web technologies and cloud solutions allows collecting and visualizing data on web pages and screens of mobile devices. The article shows a solution for collecting data from a CNC system using the OPC UA protocol and subsequent data visualization based on the FRED cloud platform in the required format with the ability to configure HMI screens.

Molecular Diagnosis of FLT3 Mutations in Acute Myeloid Leukemia Patients

Background. FLT3 gene is an important prognostic molecular marker in acute myeloid leukemia (AML). However, the detection of FLT3 mutations presents a challenge. Aim. To compare techniques used for the detection of FLT3 mutations, and to develop a test-system based on polymerase chain reaction (PCR) for quick and reliable determination of FLT3 mutation status. Materials & Methods. Bone marrow samples obtained from AML patients were subjected to examination. To detect FLT3-ITD and FLT3-TKD mutations PCR was performed with subsequent agarose gel electrophoresis visualization. The results were verified by Sanger sequencing. The data obtained using our test-system were compared with widely applied commercial kit ‘FLT3 Mutation Assay for Gel Detection’ by Invivoscribe. Results. To determine the FLT3 mutation status a PCR test was developed. This technique was validated on 22 bone marrow samples obtained from AML patients. FLT3-ITD mutation was detected in 4 patients, 3 patients showed FLT3-TKD mutation. In 1 patient both mutations were identified. These results fully corresponded to the molecular genetic analysis of FLT3, performed by ‘FLT3 Mutation Assay for Gel Detection’. The chosen technique was validated using Sanger sequencing data analysis. Conclusion. The article offers the review of all existing FLT3 mutation screening techniques and describes the experience of developing the PCR test for FLT3-ITD and FLT3-TKD mutation detection. The chosen technique is affordable and easy to use compared with the others. This study can be used as a guide for both doctors and researchers.

Visualization of Arbuscular Mycorrhizal Fungal Extraradical Hyphae and Spores Vitality and Activity.

The hyphae and spores of arbuscular mycorrhizal (AM) fungi represent an essential component in the extraradical zone due to their role in nutrients and water uptake and as propagules that allow the perpetuation of the AM symbiosis over time, respectively. However, the attention of scientific literature is usually more focused on root colonization than on the study of the extraradical components of AM fungi, especially their vital, active, or functional fractions. This chapter presents some easy-to-use alternatives for staining vital, active, or functional structures of AM fungi for their subsequent microscopic visualization, such as the application of enzyme-based stains, NADPH formation, and also nucleus staining. Some modified methods for the extraction of mycelium from the soil are also presented.

Orbitozygomatic Craniotomy for Giant Anterior Communicating Artery Aneurysm: 2-Dimensional Operative Video.

Giant intracranial aneurysms pose a significant surgical challenge because of the associated difficulty in achieving adequate visualization of the parent artery and aneurysm neck. This patient had an incidentally identified giant anterior communicating artery aneurysm. An orbitozygomatic craniotomy was performed for aneurysm exposure and aneurysmal neck dissection. Aneurysm dome opening and thrombectomy was performed to debulk the aneurysmal mass, which facilitated subsequent aneurysmal neck visualization. Sequential utilization of temporary clips of the bilateral A1 and bilateral A2 vessels reduced hemorrhage during thrombectomy. Multiple permanent clips were applied along the dissected aneurysm neck to permit occlusion. A small fracture of the aneurysm neck was identified, and cotton was applied with subsequent tamponade utilizing a fenestrated clip to maintain hemostasis. Indocyanine green fluoroscopy was used to verify parent and distant vessel patency. The patient gave informed consent for surgery and video recording. Institutional review board approval was deemed unnecessary. Used with permission from Barrow Neurological Institute, Phoenix, Arizona.

Building a Comprehensive Automated Programming Assessment System

Automated Programming Assessment Systems (APAS) are used for overcoming problems associated with manually managed programming assignments, such as objective and efficient assessing in large classes and providing timely and helpful feedback. In this paper we survey the literature and software in this field and identify the set of necessary features that make APAS comprehensive - such that it can support all key stages in the assessment process. Put differently, comprehensive APAS is generic enough to meet the demands of “any” computer science course. Despite the vast number of publications, the choice of software turns out to be very limited. We contribute by developing Edgar, a comprehensive open-source APAS which, to the best of our knowledge, exceeds any other similar free and/or open-source tool. Edgar is the result of three years of development and usage in, for the time being, eight courses dealing with various programming languages and paradigms (C, Java, SQL, etc.). Edgar supports various text-based programming languages, multi-correct multiple-choice questions, provides rich exam logging and monitoring infrastructure to prevent potential fraudulent behaviour, and subsequent data analysis and visualization of students' scores, exams, question quality, etc. It can be deployed on all major operating systems and is written in a modular fashion so that it can be adjusted and scaled to a custom fit. We comment on the architecture and present data from real-world use-cases to support these claims. Edgar is in active use today (1000+ students per semester) and it is being constantly developed with new features.

Method combining BAC film and positive staining for the characterization of DNA intermediates by dark-field electron microscopy

DNA intermediate structures are formed in all major pathways of DNA metabolism. Transmission electron microscopy (TEM) is a tool of choice to study their choreography and has led to major advances in the understanding of these mechanisms, particularly those of homologous recombination (HR) and replication. In this article, we describe specific TEM procedures dedicated to the structural characterization of DNA intermediates formed during these processes. These particular DNA species contain single-stranded DNA regions and/or branched structures, which require controlling both the DNA molecules spreading and their staining for subsequent visualization using dark-field imaging mode. Combining BAC (benzyl dimethyl alkyl ammonium chloride) film hyperphase with positive staining and dark-field TEM allows characterizing synthetic DNA substrates, joint molecules formed during not only in vitro assays mimicking HR, but also in vivo DNA intermediates.

The improved targeting of an aspirin prodrug albumin-based nanosystem for visualizing and inhibiting lung metastasis of breast cancer.

Lung metastasis is the principal reason for the majority of deaths from breast cancer. The nonsteroidal anti-inflammatory drug aspirin can prevent lung metastasis in breast tumors via inhibiting heparanase. However, the lack of specific targets and limited accumulation at the site of the tumor have thus far hindered the use of aspirin in oncotherapy. In this study, we developed the nanoplatform FA-BSA@DA and loaded it with the versatile aspirin prodrug DA to visualize and inhibit breast cancer metastasis via targeting heparanase. This nanosystem can be effectively targeted to folic acid (FA)-positive tumor cells, and would then subsequently release a high dose of DA, whose ester bond is specifically ruptured by H2O2 in the tumor microenvironment to afford the therapeutic drug aspirin and near-infrared (NIR) fluorescent reporter DCM. The released aspirin can effectively prevent breast cancer lung metastasis through the inhibition of heparanase activity, and the NIR fluorescent signals emitted from DCM can be used to monitor and evaluate the metastasis levels of breast cancer. Our results showed that the expression of heparanase was significantly decreased, and lung metastasis from breast cancer was effectively monitored and inhibited after treatment with FA-BSA@DA. Furthermore, the collaborative therapy nanoplatform FA-BSA@DA/DOX exhibited strong therapeutic effects in the treatment of breast cancer in vitro and in vivo via the introduction of doxorubicin (DOX) to the system, which resulted in an even stronger result due to its synergistic effects with aspirin. This heparanase-reliant strategy has profound significance for the extended development of nanoplatforms based on versatile aspirin prodrugs, which may offer a solution to clinically prevent breast cancer recurrence and lung metastasis.

INTELLIGENT TECHNOLOGY OF WAVELET ANALYSIS OF VIBRATION SIGNALS

During solution of engineering problems of machinery dynamics a need of revealing the harmonic components often arises in the narrow timing gate. This requires the use of wavelet-transformation oscillation methods and introduction of intelligent systems to hardware and software used in the experiment. The wavelet is considered as a short in time signal functional window, which has its internal structure in the form of a fading wavelike burst, and it is characterized by a scale of display of certain events in the field of the signal frequency spectrum, as well as and by time axis shifts. Complex-functioned continuous functions of real arguments (Daubechies wavelets, Gaussian wavelets, MHat-wavelets), complex-valued functions of real arguments (Morlet and Paul wavelets), as well as real discrete functions (HААRT- and FHat-wavelets) are used as wavelet functions. The wavelet analysis method of vibration signals is disclosed at acoustic diagnostics of machines and mechanisms. Digital implementation of discrete indications of wavelets with the subsequent visualization of results in the form of scalotons is the mathematical basis of the algorithm for procession of vibration signals. It has been suggested that engineering analysis and reconstruction of signals should be implemented by means of directed and reverse continuous wavelet conversions, which are discrete by arguments. The structural and functional scheme of the multichannel system of the intelligent wavelet analysis of vibration signals in machines has been considered. The intelligent system for study of vibration signals makes it possible to form the totality of photographic parameters, when scalotons are calculated by wavelet functions. An example of experimental implementation of the wavelet conversion method of vibration signals parameters is shown. Results of scalotons calculation are shown, when MHat-wavelet and DOG-wavelet are used.

Sublamina-specific organization of the blood brain barrier in the mouse olfactory nerve layer.

Astrocytes constitute the main glial component of the mammalian blood brain barrier (BBB). However, in the olfactory bulb (OB), the olfactory nerve layer (ONL) is almost devoid of astrocytes, raising the question which glial cells are part of the BBB. We used mice expressing EGFP in astrocytes and tdTomato in olfactory ensheathing cells (OECs), a specialized type of glial cells in the ONL, to unequivocally identify both glial cell types and investigate their contribution to the BBB in the olfactory bulb. OECs were located exclusively in the ONL, while somata of astrocytes were located in deeper layers and extended processes in the inner sublamina of the ONL. These processes surrounded blood vessels and contained aquaporin-4, an astrocytic protein enriched at the BBB. In the outer sublamina of the ONL, in contrast, blood vessels were surrounded by aquaporin-4-negative processes of OECs. Transcardial perfusion of blood vessels with lanthanum and subsequent visualization by electron microscopy showed that blood vessels enwrapped by OECs possessed intact tight junctions. In acute olfactory bulb preparations, injection of fluorescent glucose 6-NBDG into blood vessels resulted in labeling of OECs, indicating glucose transport from the perivascular space into OECs. In addition, Ca2+ transients in OECs in the outer sublamina evoked vasoconstriction, whereas Ca2+ signaling in OECs of the inner sublamina had no effect on adjacent blood vessels. Our results demonstrate that the BBB in the inner sublamina of the ONL contains astrocytes, while in the outer ONL OECs are part of the BBB.

Phase Composition and Microstructure of UHPHT Vein Glass from Giant Diamondiferous Kara Impact Crater by Raman Mapping

2D and 3D Raman mapping allows to observe “in situ” distribution of phases and relationship of minerals in rock samples, different mineral aggregates, mineral inclusions within crystals and other quantitative spectroscopic studies of mineral substances and materials. The method is realized in accumulating and processing of big data array of Raman spectra (RS) from selected areas of a studied sample with subsequent visualization in XY plane at 2D mapping or in the form of 3D experimental models with XYZ coordinates in a case of 3D Raman mapping. During the mapping different analytical data can be visualized as maps such as spatial distribution of mineral phases, degrees of their crystallinity, orientation of crystallites and their size, residual tension in minerals and inclusions and many other parameters. As 3D Raman mapping demands higher requirements to quality of samples, takes significantly long time expenditure and big data for processing, 2D Raman mapping looks to be the most effective for different mineralogical applications. In this work we demonstrate advantages of 2D Raman mapping on the example of studying of the impact ultrahigh-pressure high-temperature (UHPHT) vein glasses with melt-crystallized ultrahigh-pressure silica phase – coesite, for the first time found within diamondiferous impactites of the giant Kara meteorite crater. The mapping was produced with processing of typical spectra ranges of recognized mineral phases met in analyzed areas. As a result of the conducted studies it has been established what according to spectroscopic data coesite hangs to central parts of UHPHT silica glass located in general matrix of aluminosilicate glass. The sites of silicate glass are characterized by fully amorphous state that is obviously shown in its typical ranges. The areas with a high luminescence correspond to inclusions of smectite micro-drops in UHPHT silica glass isolations that has been confirmed with the accompanying study with use of electron probe microanalysis (EPMA), energy dispersive spectroscopy (EDS) and electron diffraction (ED). The used 2D Raman mapping to the impact vein glasses of the Kara meteorite crater allowed to reveal distribution of mineral phases in UHPHT glass, showing distribution of amorphous and crystalline phases that is undoubted advantage of the Raman spectroscopy. In full text of the paper technical features of Raman 2D mapping are shown that can be useful for similar mineral objects and materials with complex phase structure and different degree of ordering.

Visualizing a field of research: A methodology of systematic scientometric reviews.

Systematic scientometric reviews, empowered by computational and visual analytic approaches, offer opportunities to improve the timeliness, accessibility, and reproducibility of studies of the literature of a field of research. On the other hand, effectively and adequately identifying the most representative body of scholarly publications as the basis of subsequent analyses remains a common bottleneck in the current practice. What can we do to reduce the risk of missing something potentially significant? How can we compare different search strategies in terms of the relevance and specificity of topical areas covered? In this study, we introduce a flexible and generic methodology based on a significant extension of the general conceptual framework of citation indexing for delineating the literature of a research field. The method, through cascading citation expansion, provides a practical connection between studies of science from local and global perspectives. We demonstrate an application of the methodology to the research of literature-based discovery (LBD) and compare five datasets constructed based on three use scenarios and corresponding retrieval strategies, namely a query-based lexical search (one dataset), forward expansions starting from a groundbreaking article of LBD (two datasets), and backward expansions starting from a recently published review article by a prominent expert in LBD (two datasets). We particularly discuss the relevance of areas captured by expansion processes with reference to the query-based scientometric visualization. The method used in this study for comparing bibliometric datasets is applicable to comparative studies of search strategies.

Comparative in silico study of congocidine congeners as potential inhibitors of African swine fever virus.

African swine fever virus (ASFV) infection is fatal in domesticated pigs, with a mortality rate approaching 100%. This may result in economic losses and threats to food security. Currently, there are no approved vaccines or antiviral therapies for ASFV. Therefore, in this study, we evaluated congocidine congeners and a tris-benzimidazole as potential inhibitors of ASFV transcription using an in silico approach. We applied redocking of congocidine and docking of its congeners and a tris-benzimidazole to a receptor containing B-DNA with AT-motifs as a target to mimic conserved ASFV late gene promoters. Subsequently, the binding scores of DNA-ligand docked complexes were evaluated and their binding affinity was estimated. Molecular dynamics (MD) simulation was then used to assess ligand behavior within the minor groove. From our results, it is evident the less toxic congocidine congeners and tris-benzimidazole could dock to AT-rich regions significantly. Additionally, the predicted binding affinities had suitable values comparable to other experimentally determined minor groove binders, MD simulation of the docked DNA-ligand complexes and subsequent molecular trajectory visualization further showed that the ligands remained embedded in the minor groove during the time course of simulation, indicating that these ligands may have potential applications in abrogating ASFV transcription.

Novel technique with the IRIS U kit to prevent urethral injury in patients undergoing transanal total mesorectal excision.

BackgroundLow anterior resection of the rectum with total mesorectal excision (TME) has been the gold standard for the surgical treatment of rectal cancer as it has the lowest recurrence rates. The key issue while performing transanal TME (TaTME) is avoiding iatrogenic urethral injury. We introduce our surgical technique for TaTME.Surgical techniqueIntraurethral indocyanine green injection using the IRIS U kit with subsequent visualization under NIR was safely utilized during the TaTME. We were able to easily detect and visualize the IRIS urethral kit. The prostatic segment of the urethra can be identified in real-time using the infrared illumination system urethral kit (IRIS U kit).BenefitsThe prostatic segment of the urethra was easily and quickly identified by the green fluorescence during TaTME.ConclusionOur TaTME technique is an easy and feasible approach that provides real-time urethral images.

Therapeutic use of tetrasodium ethylenediaminetetraacetic acid solution for treatment of subcutaneous ureteral bypass device mineralization in cats.

BackgroundSubcutaneous ureteral bypass (SUB) device placement is an increasingly popular treatment option for decompression of ureteral obstruction in cats. Mineralization occlusion of the device occurs in a minority of cases but is the most common complication.ObjectiveTo evaluate a 2% tetrasodium ethylenediaminetetraacetic acid (tEDTA) solution for treatment of mineralization occlusion in cats with SUBs.AnimalsSix client‐owned cats (8 obstructed devices).MethodsCase series. Each cat was found to have device occlusion based on a combination of ultrasound examination, SUB irrigation, and failure to identify another cause of device obstruction. Each SUB was drained, irrigated using sterile saline, and infused with 1‐2 mL of 2% tEDTA solution. Success was defined as normalization of flow during subsequent ultrasound visualization while irrigating. The volume and frequency of tEDTA instillations, time to achieve device patency, follow‐up biochemical and ultrasound findings, and future reobstruction events were recorded.ResultsResolution of mineralization was documented in all 8 SUBs. Reobstruction events occurred in 2 cats, all of which resolved after additional tEDTA infusions, but 1 cat ultimately required device exchange at 356 days from the first tEDTA infusion. In 1 cat, a single infusion was prematurely discontinued because of persistent pelvic dilatation after 1.25 mL of tEDTA had been instilled. No complications were observed.Conclusions and Clinical ImportanceTetrasodium EDTA infusions can be safely considered as a treatment option for mineralized SUB devices in cats. This solution was easily infused, well tolerated, and avoided the need for SUB device exchange in the majority of cats in which it was used.

User-perceived Information Visualization Method in Human Body Acupoint System

Information visualization is a trend and research hotspot in various fields. This paper constructs a user-perceived information visualization framework and applies it to the human acupoint system. A user-perceptual visualization method of three-layer architecture is proposed. A unified storage method and access interface are provided in the data unified layer to effectively manage data information. Then, in the user perception layer, the K-means clustering method is used to generate the expected interface of the system, which reduces the processing tasks of the subsequent visualization process. The visual implementation layer forms an actual interface for interface design such as interaction, navigation, and the like of the content of the intended interface. Finally, an application example of the acupoint system is given, which proves that the method can complete the visualization from the source data to the actual interface.

Remote control of a robotic prosthesis arm with six-degree-of-freedom for ultrasonic scanning and three-dimensional imaging

Robotic ultrasound systems have turned into clinical use over the past decades, increasing precision and quality of medical operations. In this paper, we propose a robot-assisted and tele-controlled ultrasound scanning system for three-dimensional imaging. A six degree-of-freedom (DOF) robot arm acting as an upper-limb prosthesis remotely controlled by an operator drove the ultrasound probe to scan the skin surfaces. Via internet, the operator in a distant place monitored the robotic prosthesis and the patient through four video cameras on-site. According to the real-time video streams, the operator could manipulate a joystick for sending instructions from the remote operating terminal to the system on the patient side to adjust the probe motions. In this way, the safety and accuracy of the proposed system could be guaranteed. During the probe scanning, the acquired B-scans and their positional data were transferred to the remote workstation for the subsequent volume reconstruction and visualization. In-vitro and in-vivo experiments were conducted to validate the feasibility and performance of the proposed system. The quantitative experimental results show that the error for volume measurement was less than 1.1%, indicating that our system could accurately and flexibly control the probe scanning and produce 3D ultrasound images with high quality.

Current Techniques of Intravascular Imaging and Implantation of Bioresorbable Coronary Vascular Scaffolds (Stents)

Introduction. The use of intravascular imaging techniques, in particular intravascular ultrasound, makes it possible to reduce the number of subsequent revascularisations. The method of optical coherence tomography (ОСТ), due to its high resolution accuracy, makes it possible to establish and assess the edge dissection, stent thrombosis, tissue prolapse, and strut fracture and malposition. This paper aims to assess the impact of routine use of OCT on long term outcomes in patients with scaffolds implanted.Materials and methods. 32 stenting procedures with the use of bioresorbable coronary intravascular scaffolds (Absorb, Abbot Vascular) were performed in 2014-2015 with subsequent visualisation and assessment with optical coherence tomography. In the control group (n=16) scaffolds were implanted without the use of OCT under traditional contrast enhanced X-ray imaging. The analysis of the combination of outcomes that included subsequent emergency revascularisation in the target artery, cardiac death, myocardial infarction and 12 months post-op OCT imaging data, served as the end.Results. The total of 55 BVS were implanted, 22 in the OCT group and 23 in control. Suboptimal results were registered in eight cases out of 16 in the OCT group (50%). These included: one dissection and atherosclerotic plaque prolapse — implantation of a second BVS and postdilatation, one eccentric stent expansion — a model for acute thrombosis, resolved with postdilatation, and stent underexpansion in the remaining six. Once the OCT revealed the intima dissection following predilatation; this was stabilised with the implantation of a second scaffold. The examination results at 12 months follow up established that there were 3.215 more scaffold restenoses in the control group.Conclusion. The cutting edge technique of intravascular revascularisation with bioresorbable coronary scaffolds requires careful preparation and intraoperative control. The results of our study support the use of intravascular imaging techniques as methods of choice for the assessment of the expansion, areas of dissection, thrombosis and scaffold eccentricity.