Subsequent Transformation Research Articles

Porous and Meltable Metal-Organic Polyhedra for the Generation and Shaping of Porous Mixed-Matrix Composites.

Here, we report the synthesis of BCN-93, a meltable, functionalized, and permanently porous metal-organic polyhedron (MOP) and its subsequent transformation into amorphous or crystalline, shaped, self-standing, transparent porous films via melting and subsequent cooling. The synthesis entails the outer functionalization of a MOP with meltable polymer chains: in our model case, we functionalized a Rh(II)-based cuboctahedral MOP with poly(ethylene glycol). Finally, we demonstrate that once melted, BCN-93 can serve as a porous matrix into which other materials or molecules can be dispersed to form mixed-matrix composites. To illustrate this, we combined BCN-93 with one of various additives (either two MOF crystals, a porous cage, or a linear polymer) to generate a series of mixed-matrix films, each of which exhibited greater CO2 uptake relative to the parent film.

Efficient light propagation algorithm using quantum computers

Quantum algorithms can potentially overcome the boundary of computationally hard problems. One of the cornerstones in modern optics is the beam propagation algorithm, facilitating the calculation of how waves with a particular dispersion relation propagate in time and space. This algorithm solves the wave propagation equation by Fourier transformation, multiplication with a transfer function, and subsequent back transformation. This transfer function is determined from the respective dispersion relation, which can often be expanded as a polynomial. In the case of paraxial wave propagation in free space or picosecond pulse propagation, this expansion can be truncated after the quadratic term. The classical solution to the wave propagation requires O(NlogN) computation steps, where N is the number of points into which the wave function is discretized. Here, we show that the propagation can be performed as a quantum algorithm with OlogN2 single-controlled phase gates, indicating exponentially reduced computational complexity. We herein demonstrate this quantum beam propagation method (QBPM) and perform such propagation in both one- and two-dimensional systems for the double-slit experiment and Gaussian beam propagation. We highlight the importance of the selection of suitable observables to retain the quantum advantage in the face of the statistical nature of the quantum measurement process, which leads to sampling errors that do not exist in classical solutions.

Soil Microbiome of Abandoned Plaggic Podzol of Different-Aged Fallow Lands and Native Podzol in South Taiga (Leningrad Region)

The soil microbiome is composed of various communities that play an important role in the existence of ecosystem services and the sustainable functioning of ecosystems under high anthropogenic loads. The transition of soils to a fallow state and their subsequent transformation lead to a notable alteration in the taxonomic composition of the soil microbiome, impacting the biochemical processes within the soil and its fertility levels. The object of this study comprised different-aged fallow soils of the southern taiga in the vicinity of Ban’kovo village, Leningrad region. The method comprising the high-throughput sequencing of 16S rRNA gene fragments using an Illumina MiSEQ sequencer was used to analyze the microbial community. The general processing of sequences was carried out with the dada2 (v1.14.1) package. It was found that the morphological organization of fallow soils has significant differences from the native podzol. In fallow soils, there are signs of leaching expressed in the accumulation of leached mineral particles, which indicates the degradation of the fallow–arable horizon. At the same time, there is a decrease in the content of P2O5 and K2O and an increase in the content of N-NH4 and N-NO3 in fallow soil. The analysis of alpha diversity index values showed that the highest level of alpha diversity in the microbial community is characteristic of 40-year-old soil, the alpha diversity index decreased with the increasing time of the fallow state, and the lowest alpha diversity index was observed in the native podzol. According to the values of the beta diversity index, a high correlation between the soil microbiome and the physicochemical characteristics of the soil was revealed, which indicates the formation of functional specialization in the studied microbial communities. As a result of the study of the taxonomic composition of microbial communities in fallow soils, it was found that the most represented microbial communities in fallow soils belong to Nitrosomonadaceae (Pseudomonadota), Mycobacterium (Actinobacteria), Nitrospira (Nitrospirota), and Luteolibacter (Verrucomicrobiota). The duration of post-agrogenic transformation is the leading factor influencing the changes in microbial communities; so, with an increase in the time that soils were in a fallow state, an increase in the oligotrophic microbial community was observed.

Enhanced microbubble-mediated cavitation by using acoustic droplet vaporization

Acoustic cavitation serves various therapeutic purposes, with both microbubbles and phase-transit droplets serving as potential cavitation nuclei. This project focused on investigating the intricate interaction between microbubbles and droplets and the resultant impact on acoustic cavitation.To facilitate this investigation, microbubbles with a negative potential of −51.58 mV were prepared, while droplets were endowed with a positive potential of +4.22 mV, facilitating the formation of microbubble-droplet clusters. Microscopic analysis was employed to observe the morphology of these clusters, revealing an initial mean size of 4.85 µm before ultrasound activation and subsequent transformation into larger bubbles with a mean size of 12.54 µm after ultrasound activation. The experimental approach involved the use of varying driven voltages to generate ultrasound waves with different intensities. A passive cavitation detector (PCD) was then employed to capture cavitation signals. Results demonstrated that, when maintaining a microbubble ratio of 50% and varying droplet ratios from 0% to 50%, the driven voltage at which the cavitation peak appeared decreased from 180 mV to 140 mV. Additionally, the mean cavitation intensity exhibited an initial increase from 0.58 to 0.78, followed by a subsequent decrease from 0.78 to 0.63 after normalization.This study provided valuable insights into the dynamic interactions between microbubbles and droplets. Specifically, it showed that microbubble cavitation transferred energy to droplets, leading to the generation of larger bubbles and higher cavitation intensity. Nevertheless, the observed cavitation intensity and threshold were contingent upon the ratios of microbubbles and droplets. Notably, to achieve an ideal cavitation intensity, the energy generated by microbubble cavitation must be substantial enough to activate all droplets, inducing acoustic droplet vaporization (ADV) and cavitation.

Genomic Analysis Points to Multiple Genetic Mechanisms for Non-Transformable Campylobacter jejuni ST-50.

Campylobacter jejuni and Campylobacter coli are well known for their natural competence, i.e., their capacity for the uptake of naked DNA with subsequent transformation. This study identifies non-transformable C. jejuni and C. coli strains from domestic animals and employs genomic analysis to investigate the strain genotypes and their associated genetic mechanisms. The results reveal genetic associations leading to a non-transformable state, including functional DNase genes from bacteriophages and mutations within the cts-encoded DNA-uptake system, which impact the initial steps of the DNA uptake during natural transformation. Interestingly, all 38 tested C. jejuni ST-50 strains from the United States exhibit a high prevalence of non-transformability, and the strains harbor a variety of these genetic markers. This research emphasizes the role of these genetic markers in hindering the transfer of antimicrobial resistance (AMR) determinants, providing valuable insights into the genetic diversity of Campylobacter. As ST-50 is a major clone of C. jejuni globally, we additionally determined the prevalence of the genetic markers for non-transformability among C. jejuni ST-50 from different regions of the world, revealing distinct patterns of evolution and a strong selective pressure on the loss of competence in ST-50 strains, particularly in the agricultural environment in the United States. Our findings contribute to a comprehensive understanding of genetic exchange mechanisms within Campylobacter strains, and their implications for antimicrobial resistance dissemination and evolutionary pathways within specific lineages.

Protection-Free, Two-step Synthesis of C5-C Functionalized Pyrimidine Nucleosides.

A simple, reliable, and efficient method for the gram-scale chemical synthesis of pyrimidine nucleosides functionalized with C5-carboxyl, nitrile, ester, amide, or amidine, starting from unprotected uridine and cytidine, is described. The protocol involves the synthesis of 5-trifluoromethyluridine and 5-trifluoromethylcytidine with Langlois reagent (CF3 SO2 Na) in the presence of tert-butyl hydroperoxide and subsequent transformation of the CF3 group to the C5-C 'carbon substituents' under alkaline conditions. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Synthesis and characterization of 5-trifluoromethyluridine (5-CF3 U) and 5-trifluoromethylcytidine (5-CF3 C) Basic Protocol 2: Conversion of 5-CF3 U and 5-CF3 C to several C5-substituted ribonucleosides.

Deciphering orbital hybridization in heterogeneous catalysis

AbstractThe catalytic coordinate is essentially the evolving frontier orbital interaction while feeding with catalytic materials and adsorbates under proper reaction conditions. The heterogeneous catalytic reaction mechanism involves the initial adsorption and activation of reactants, subsequent intermediate transformation, final target product desorption, and regeneration of catalytic materials. In these catalytic processes, interaction modulations in terms of orbital hybridization/coupling allow an intrinsic control on both thermodynamics and kinetics. Concerned charge transfer and redistribution, orbital splitting and rearrangement with specific orientation, and spin change and crossover pose a formidable challenge on mechanism elucidation; it is hard to precisely correlate the apparent activity and selectivity, let alone rational modulations on it. Therefore, deciphering the orbital couplings inside a catalytic round is highly desirable and the dependent descriptor further provides in‐depth insights into catalyst design at the molecule orbital level. This review hopes to provide a comprehensive understanding on orbital hybridizations, modulations, and correlated descriptors in heterogeneous catalysis.

Secondary School Students’ Response to Learning the Concept of Destruction and Transformation of an Artwork into Another Artwork in the Visual Arts Class

This empirical study aimed to introduce the concept of destruction and transformation into secondary school art education as a potential way of incorporating conceptual and contemporary artistic practices. Experts have highlighted the absence of such content in art education, emphasising the responsibility of teachers to integrate it into the learning process thoughtfully. To encourage students to engage in profound thinking about destruction and internalise the essence of conceptual art through practical experiences, we conducted a study at a Ljubljana secondary school. Here, students created their initial artwork (ready- made), a prerequisite for its subsequent destruction and transformation into a new artwork. We included two first-year classes, exploring 1) their response to the presented artworks aligned with the researched concept, 2) their attitude towards destroying and transforming their initial art- work, and 3) their response to the concept of destruction and transformation through their creative practice. Over four school hours, we gathered data through both qualitative and quantitative research techniques. The results showed that in-depth discussions of conceptual foundations led to a predominantly positive student attitude towards contemporary artworks. They not only understood but also internalised the essence of these artworks, which was vividly reflected in their practical artistic expressions. In conclusion, the study effectively introduced the concept of destruction and transformation into secondary school art education, promoting a deeper understanding of contemporary art among students while cultivating their creative and critical thinking skills.

THE PHYSICOCHEMICAL INVESTIGATION OF THE ZHURAVLINY LOG KAOLIN. PART 2

MAS NMR spectra of concentrated kaolin of the Zhuravliny Log deposit (Chelyabinsky district, Russia) and its heated products were studied. In a raw concentrated kaolin, a sharp absorption of 29Si NMR was observed at –91.2 ppm, which corresponded to the characteristic signal of layered structure in silicates with no Al tetrahedra (Q3(0Al)). In the heated at 800 °C samples, the center of the Si resonance shifted to –102.9 ppm. The peak became broader and partially asymmetric that might ascribe the disordered layered structure of metakaolin (Q3 state). At 900 °C the spectral line center was at –106.8 ppm (Q4 Si) related to the presence of amorphous SiO2 or cristobalite. The 27Al NMR spectrum of a raw kaolinite consisted of only a single sharp resonance centered at 1.3 ppm which was assigned to AlVI. Heat treatment at a temperature above 500 °C led to the partial destruction of the layered structure. Two new lines appeared at 58.1–60.2 and 29.0–32.9 ppm which were attributed to both Al atoms in tetrahedral and penta-coordinated positions, respectively. The signal intensity of AlIV peak increased rapidly. The raw kaolinite had particles with angular edges of pseudo-hexagonal form which suggested a well-ordered mineral. The burning temperature increasing (800–1200 °C), flakes got the more rounded boundaries. At 1000–1200 °C flakes were agglomerated. According to the dilatometry curve for kaolin, in 500–650 °C and 630–880 °C intervals there was a small shrinkage which was related to dehydroxylation of kaolinite and structural reorganization of metakaolinite, respectively, with the subsequent transformation into the Si-Al spinel phase above 900 °C.

Synthesis and isolation of a cyclic bis-vinyl germylene via a diazoolefin adduct of germylene dichloride.

Since the successful isolation of various stable diazoolefins, an array of complexes containing these promising ligands have been synthesized. We herein report the synthesis, characterization, and structures of neutral group 14 diazoolefin complexes and the subsequent transformation into a new cyclic bis-vinyl germylene.

Genome‐wide characterization of trihelix genes reveals Cqtrihelix23 enhances the salt tolerance in quinoa (Chenopodium quinoa)

AbstractSoil salinity poses an escalating threat to global environmental sustainability, agricultural productivity, and food safety. Quinoa (Chenopodium quinoa), recognized as a halophyte, has emerged as a promising crop due to its high nutritional value and stress resistance. Nevertheless, the current understanding of salt tolerance genes in quinoa remains incomplete. This comprehensive study aimed to identify the quinoa trihelix family and families associated with salt stress, including Na+/H+ antiporter (NHX) and calcineurin B‐like (CBL). Through expression analysis, Cqtrihelix23 was identified as responsive to salt stress. Subsequent transient transformation experiments revealed that Cqtrihelix23 enhanced quinoa's tolerance to salt stress by promoting root development, maintaining the antioxidant system, and reducing the Na+/K+ ratio. Additionally, it was discovered that Cqtrihelix23 upregulated the expression of CqCBL10 and CqNHX4. Further protein interaction experiments confirmed the interaction between Cqtrihelix23, CqCBL10, and CqNHX4. Notably, CqCBL10 and CqNHX4 also contributed to salt stress resistance, and in combination with Cqtrihelix23, they synergistically enhanced salt stress tolerance. In conclusion, this study highlights the significance of Cqtrihelix23, CqCBL10, and CqNHX4 as key contributors within the regulatory network associated with quinoa's salt tolerance. These findings lay a solid groundwork for the development of salt‐tolerant quinoa varieties.

Supramolecular "baking powder": a hexameric halogen-bonded phosphonium salt cage encapsulates and functionalises small-molecule carbonyl compounds.

We report a hexameric supramolecular cage assembled from the components of a Wittig-type phosphonium salt, held together by charge-assisted halogen bonds. The cage reliably encapsulates small polar molecules, including aldehydes and ketones, to provide host-guest systems where components are pre-formulated in a near-ideal stoichiometry for a mechanochemical base-activated Wittig olefination. These pre-formulated solids represent a proof-of-principle for a previously not reported supramolecular design of solid-state reactivity in which the host for molecular inclusion also acts as a complementary reagent for the subsequent chemical transformation of an array of guests. The host-guest solid-state complexes can act as supramolecular surrogates to their Wittig olefination vinylbromide products in a Sonogashira-type coupling that enables one-pot mechanochemical conversion of an aldehyde to an enediyne.

Registers of Educational Programmes as a Tool for Developing the Industry’s Human Resources Potential

The purpose of the article is to present a toolkit for analyzing changes in the system of library training, its subsequent adjustment and transformation into an effective tool for the development of the industry. The basis of this work is the monitoring of the functioning of the system of university and additional professional education (APE) in the field of “Library and Information Activities”.To solve this task the specialists of the Russian State Library have developed and created two registers: The Register of basic educational programmes of higher education, implemented in the direction of “Library and Information Activity”, and the Register of existing educational programmes of advanced training and professional retraining for library specialists. The registers are placed in the “Library Professionals” section of the federal state information system “National Electronic Library”. A study of these registers has been carried out and their expert evaluation has been made. The result of this work may be the creation of a “map” of higher and additional professional education of Russian library professionals.The register of basic educational programmes of higher education implemented in the field of “Library and Information Activity” contains details about 73 educational programmes of higher education of institutions from 32 subjects of the Russian Federation. The register of educational programmes of professional development and professional retraining for library specialists includes information about 241 educational programmes of additional professional development (29 professional retraining programmes and 212 professional development programmes). These programmes are implemented in 34 subjects of the Russian Federation.The programmes of APE are analyzed. The author’s methodology of evaluation of professional retraining programmes is presented. The minimum number of points that a professional retraining programme received according to the results of the examination was 6, the maximum number of points was 43.33 (out of 50 possible).Conclusions on the results of the expert evaluation are given. The prepared registers are presented as one of the elements of the information and educational platform of Russian libraries.

ALEVİ SİYASALLAŞMASI VE DIŞ POLİTİKA: ALEVİ SİYASİ PARTİLERİNDE KİMLİK-ULUSLARARASI POLİTİKA BAĞLANTISI

The foreign policy and international relations perceptions of sub-state groups have recently begun to be discussed in the literature. The central role of the state is eroding and non- and sub-state actors are emerging as influential factors in international politics. Identity is the most dominant factor in the foreign policy perspectives of these actors. This study examines Alevi politicization in Turkey in relation to foreign policy and identity. The politicization of Alevism in Turkey has accelerated since the 1950s with the urbanization of Alevis and their rapprochement with leftist ideologies. The tension between Alevism and the state since the Ottoman period has also fed this process. As a result of this politicization, the Unity Party (1966-1980) and the Peace Party (1996-1999) were established as Alevi parties. The relationship that both parties established with their Alevi base, their perception of domestic politics and their interpretation of international developments influenced their identities. This study examines the relationship between Alevi identity and foreign policy based on the parties' programs. The study aims to contribute to the literature by making three claims. The first is that sub-state entities can also make foreign policy. Sub-state identities formulate their own foreign policy concepts independently of governments and successfully integrate domestic and international developments into their political programs. Second, domestic and international developments have a transformative impact on the politicization of Alevis. The birth of the Alevi political movement following urbanization and its subsequent ideological transformation with leftist politics illustrates this. As a result, there has been a rupture between the Alevi political movement and its traditions and institutions. Third, Alevi politicization in the 1960s and 1990s followed different paths. The first was the process of individual Alevi politicization in the bipolar order of the Cold War conditions, which shrouded micro debates, while the second was the emergence of an Alevi political movement as a result of the increased emphasis on identity after the end of the Cold War.

ALCHEMICAL SYMBOLISM OF THE NOVELLA “THE GOLDEN POT: A MODERN FAIRY TALE” BY E. T. A. HOFFMANN

The aim of this study is to analyse the symbolism of E.T.A. Hoffman’s novella “The Golden Pot” within the framework of the alchemical discourse. The researchers employed historical-genetic, structural-semantic methods and the method of motivic analysis to explore the elements of medieval alchemical symbolism in the novella, namely: the motif of the hero’s initiation and his subsequent spiritual transformation, the symbols of snake, elemental spirits (salamander and dragon), manuscripts, blood, apples, garden, four elements, palm and cypress, as well as the symbolism of colour – red, black, white, gold and bronze. Results of the research suggest that the medieval theory of alchemy corresponded to the image of achieving creative harmony and spiritual transformation in German Romanticism.

Direct quantification of 1,1-dimethylhydrazine transformation products in sandy soil by thermal desorption gas chromatography – Tandem mass spectrometry

The use of rocket propellants based on unsymmetrical dimethylhydrazine (UDMH) in launch vehicles and boosters poses a threat to the environment due to the release of UDMH into soils and its subsequent transformation into a variety of nitrogen-containing compounds, including toxic and carcinogenic ones. The present proof-of-concept study proposes a novel approach to screening and simultaneous determination of a wide range of nitrogen-containing UDMH transformation products in soils, based on direct thermal desorption (TD) as a solvent-free sample extraction technique coupled with GC–MS/MS analysis.Optimal TD conditions consisting of the incubation of 5 mg sample at 300 °C for 30 min, allowed attaining high recoveries (80–100 %) for the most tested compounds and developing the method for quantification of fifteen acyclic and heterocyclic UDMH transformation products in sandy soil with LODs of 0.3–7.8 µg kg−1. The matrix matched calibration and introduction of the internal standard (pyridine‑d5) ensured high accuracy (75–100 %) and precision (RSD < 15 %) of the analysis. Along with high sensitivity and accuracy, the main advantages of the developed method are exceptional simplicity of sample preparation, low amount of toxic sample, high automation degree, and environmental friendliness.The developed method was successfully tested in the analysis of seven real soil samples taken from the Baikonur cosmodrome operational zone (Kazakhstan). The use of TD-GC–MS analysis in full spectrum scanning mode allowed for tentative identification of 62 UDMH transformation products including several compounds not yet described in the literature.

TGF-β and BMP signaling are associated with the transformation of glioblastoma to gliosarcoma and then osteosarcoma.

Gliosarcoma, an isocitrate dehydrogenase wildtype (IDH-WT) variant of glioblastoma, is defined by clonal biphasic differentiation into gliomatous and sarcomatous components. While the transformation from a glioblastoma to gliosarcoma is uncommon, the subsequent transformation to osteosarcoma is rare but may provide additional insights into the biology of these typically distinct cancers. We observed a patient initially diagnosed with glioblastoma, that differentiated into gliosarcoma at recurrence, and further evolved to osteosarcoma at the second relapse. Our objective was to characterize the molecular mechanisms of tumor progression associated with this phenotypic transformation. Tumor samples were collected at all 3 stages of disease and RNA sequencing was performed to capture their transcriptomic profiles. Sequential clonal evolution was confirmed by the maintenance of an identical PTEN mutation throughout the tumor differentiation using the TSO500 gene panel. Publicly available datasets and the Nanostring nCounter technology were used to validate the results. The glioblastoma tumor from this patient possessed mixed features of all 3 TCGA-defined transcriptomic subtypes of an IDH-WT glioblastoma and a proportion of osteosarcoma signatures were upregulated in the original tumor. Analysis showed that enhanced transforming growth factor-β (TGF-β) and bone morphogenic protein signaling was associated with tumor transformation. Regulatory network analysis revealed that TGF-β family signaling committed the lineage tumor to osteogenesis by stimulating the expression of runt-related transcription factor 2 (RUNX2), a master regulator of bone formation. This unusual clinical case provided an opportunity to explore the modulators of longitudinal sarcomatous transformation, potentially uncovering markers indicating predisposition to this change and identification of novel therapeutic targets.

Biogenic Vaterite Calcium Carbonate-Silver/Poly(Vinyl Alcohol) Film for Wound Dressing.

Vaterite, a spherical polymorph of CaCO3, shows potential as a carrier for the stable and controlled release of silver nanoparticles (AgNPs), preventing their aggregation or loss of efficacy during application. Furthermore, the embedding of CaCO3-Ag in a poly(vinyl alcohol) (PVA) matrix helps effectively encapsulate and protect the CaCO3-Ag microspheres and provides mechanical stability for better contact with the wound surface. This article focuses on the fabrication of an antimicrobial and biocompatible absorbent film embedded with precipitated biogenic vaterite CaCO3-Ag microspheres. The impact of vaterite CaCO3-Ag on the physical, chemical, nanomechanical, biocompatibility, and antimicrobial properties of the PVA films was investigated. The morphology study revealed a bilayer film structure with an inactive and active surface containing homogeneously distributed vaterite CaCO3-Ag. The X-ray photoelectron spectroscopy (XPS) analysis of the spin-orbit splitting in the Ag 3d5/2 and Ag 3d3/2 peaks indicated the presence of both metallic and ionic states of silver in vaterite CaCO3-Ag prior to its incorporation into the PVA polymer matrix. However, upon embedding in the PVA matrix, a subsequent transformation to solely ionic states was observed. The nanomechanical properties of PVA improved, and the reduced modulus and hardness increased to 14.62 ± 5.23 and 0.64 ± 0.29 GPa, respectively. The films demonstrate a significant activity toward Gram-negative Escherichia coli bacteria. The release of AgNPs was studied in both open and closed systems at pH 6, mimicking the pH environment of the wound, and it demonstrated a dependency on the type of capping agent used for synthesis and loading of AgNPs. The results further revealed the biocompatibility of the prepared films with human dermal fibroblast cells at a concentration of ≤5 mg/mL, making them applicable and functional for wound dressing applications.

The transformation of multifunctional bio-patch to hydrogel on skin wounds for efficient scarless wound healing

Hydrogels have been widely used in various biomedical applications, including skin regeneration and tissue repair. However, the capability of certain hydrogels to absorb exudate or blood from surrounding wounds, coupled with the challenge in their long-term storage to prevent bacterial growth, can pose limitations to their efficacy in biological applications. To address these challenges, the development of a multifunctional aloin-arginine-alginate (short for 3A) bio-patch capable of transforming into a hydrogel upon absorbing exudate or blood from neighboring wounds for cutaneous regeneration is proposed. The 3A bio-patch exhibits outstanding features, including an excellent porous structure, swelling properties, and biodegradability. These characteristics allow for the rapid absorption of wound exudates and subsequent transformation into a hydrogel that is suitable for treating skin wounds. Furthermore, the 3A bio-patch exhibits remarkable antibacterial and anti-inflammatory properties, leading to accelerated wound healing and scarless repair in vivo. This study presents a novel approach to the development of cutaneous wound dressing materials.

Formation of new narratives in the field of regional economic research

The article studies evolution of main approaches to the study of regional economy. Over the past half a century, the research paradigm in the area of economic sciences has undergone significant changes: the theories of regional economics have changed, significantly evolving, complementing, and mutually reinforcing each other. The original objectives of regional economic research have changed, responding to modern challenges of the world community development and global crisis phenomena. As a result, as shown in the article, in recent years focus of research has shifted significantly towards analyzing strategic aspects and directions of regional development, as a result of which the relevant concepts have become less economics-centric. An important pattern of modernity has also become a new wave of scientific and technological progress, which determined regional innovation systems development and their subsequent digital transformation that also put a significant imprint on the paradigm of regional economy research.