Subsequent Measurements Research Articles

Adalimumab Autoantibodies in Uveitis Patients: Do We Need Routine Drug Monitoring?

Background: Adalimumab, an anti-TNF-α biologic agent, has emerged as a principal treatment option for patients with non-infectious uveitis. The influence of adalimumab anti-drug antibodies (AAA) on the efficacy of adalimumab therapy is not yet fully understood. We aim to understand their clinical implications in the context of therapeutic drug monitoring and the factors contributing to the formation of these antibodies. Methods: We conducted a retrospective analysis of 114 patients with non-infectious uveitis who developed AAA while undergoing adalimumab therapy. Results: Among the 114 AAA-positive uveitis patients, a significant correlation was observed between AAA levels and reduced adalimumab serum levels (r = −0.58, p < 0.001). The mean time to AAA detection was 2.1 years (range 0.1–11.9 years), with 45.6% of cases identified through routine testing. If AAA levels were initially low, subsequent measurements for AAA were more likely to become negative during treatment (r = 0.63, p < 0.001). Higher AAA concentrations were associated with a shorter time to detection (r = −0.27, p = 0.01) and younger age (r = −0.21, p = 0.03). There was a trend, though no significant influence, of concomitant immunosuppression with prednisolone ≤ 7.5 mg or methotrexate on antibody formation (p = 0.18). No significant difference was observed in AAA levels between uveitis subtypes. Conclusions: Higher AAA concentrations are associated with lower adalimumab serum levels in uveitis patients. Routine clinical testing is essential for optimal therapeutic drug monitoring to prevent early loss of effectiveness.

Cryopreserved PBMCs can be used for the analysis of mitochondrial respiration and serve as a diagnostic tool for mitochondrial diseases.

Mitochondrial diseases are severe, inherited metabolic disorders that affect the paediatric population. They affect the functioning of mitochondrial oxidative phosphorylation (OXPHOS) apparatus either directly or indirectly. Since mutations in mtDNA are responsible for only 25% of paediatric cases and next-generation sequencing does not always provide a conclusive diagnosis, the biochemical approach still represents a valuable tool in diagnostics. Mitochondrial defects can be identified in tissue biopsies (muscle or skin). However, they also often manifest in peripheral blood cells. We developed a protocol for isolation and cryopreservation of peripheral blood mononuclear cells (PBMCs) from 5 ml of children's blood using Ficoll centrifugation which can be utilised for subsequent functional measurements on thawed samples. Furthermore, we evaluated the diagnostic utility of the optimised high-resolution oxygraphy protocol using digitonin-permeabilized cryopreserved PBMCs on 47 samples from patients with confirmed or suspected mitochondrial disease. Overall, the diagnosis was confirmed in 72% of cases, while the analysis of cryopreserved PBMCs provided a false negative outcome in 13% of cases. Our study demonstrates a sensitive, fast, and non-invasive approach for the diagnostics of various types of mitochondrial disorders, especially those of nuclear genetic origin manifesting in paediatric patients.

Conformal leaky wave antenna based on gap waveguide technology

Abstract Due to structural and aerodynamic limitations, conformal antennas are increasingly sought after in antenna design. Gap waveguide technology has also gained traction for its potential in leaky wave antenna development. This paper proposes a novel conformal leaky wave antenna utilizing this emerging gap waveguide technology. Prior research has not extensively explored the radiation characteristics of such antennas on curved and conformal surfaces. This work addresses this gap by investigating the design procedure, radiation mechanism, and optimization methods for leaky wave antennas on conformal surfaces. The proposed antenna achieves a gain of approximately 18 dB and a scanning angle ranging from 1 to 33 degrees within the frequency band of 9.5 GHz to 12 GHz. Fabrication and subsequent measurements of the antenna were conducted, with the results demonstrating good agreement with the simulations.

Quantification of Rapid Cooling of Glycerol/Water Solutions Based on Photoluminescence from Thioflavin T.

Rapid cooling to a solid state allows intermediates in chemical and biomolecular processes that occur in solution near room temperature to be trapped for subsequent measurements by magnetic resonance spectroscopies, electron microscopy, or other techniques. In time-resolved solid state nuclear magnetic resonance and rapid freeze-quench electron paramagnetic resonance studies, solutions are typically frozen by spraying into a cold bath or onto a cold metal surface. Although simulations suggest freezing on millisecond or submillisecond time scales, direct experimental measurements of cooling rates have been elusive. Here, we describe a method for quantification of rapid cooling rates based on measurements of temperature-dependent photoluminescence from thioflavin T (ThT). In our experiments, a jet of ThT solution in glycerol/water, with 10.8 m/s jet velocity and 30 μm diameter, freezes on a cold, rotating copper surface. Images of ThT photoluminescence on the copper surface indicate that the cooling rate of the solution increases linearly with the surface velocity over the 0.45-6.2 m/s range. At surface velocities greater than 3.8 m/s, the time to cool from 300 to 260 K or from 300 to 230 K is less than 100 μs or less than 700 μs. The experimental results do not agree quantitatively with calculations in which a layer of glycerol/water cools by thermal conduction when suddenly brought in contact with a cold copper surface. Discrepancies between experimental results and simplistic calculations illustrate the importance of direct measurements of cooling rates.

2D and 3D surface roughness parameters of surfaces machined by EDM

In electro discharge machining (EDM or spark erosion) the roughness of the machined surface plays a very important role in the applicability of the process. The EDM machined surfaces are near isotropic, so the 3D measured surface roughness parameters can be compared to the parameters measured by 2D method. This paper reports on the results of research on the comparison of 2D and 3D surface roughness parameters of EDM machined surfaces of tool steels. Based on the results of the performed cutting experiments and the subsequent measurements, conclusions will be drawn regarding the connection between the 2D and 3D surface parameters and the extent of roughness of steel surfaces machined by two type electrode material.

Cut-offs for relapse detection in men with stage I testicular germ cell tumors during active surveillance within a prospective multicentre cohort study using either raw or housekeeper normalized miR-371a-3p serum levels

PurposeMiR-371a-3p represents a novel liquid biomarker that detects all histologies of germ-cell tumors (GCT) except teratoma. However, it is currently unclear whether miR-371a-3p results obtained directly from RT-PCR (raw Cq) or normalized for housekeeper genes and transformed into the relative quantity (RQ) value should be used and at what cut-off level. The purpose of this research was to evaluate, which values should be used, and a potential cut-off level for relapse-detection to inform subsequent studies. Materials and MethodsWe applied a CE-certified qRT-PCR test to measure miR-371a-3p at each follow-up visit during active surveillance in 34 men with stage I testicular GCT. MiR-371a-3p levels were calculated by the ΔΔ method. ResultsAbout 18 Patients had pure seminoma and 16 had mixed or nonseminomatous testicular GCT. Recurrences were detected in 10 patients and were correctly identified by both raw and housekeeper-normalized miR-371a-3p serum levels. The raw Cq, with a cut-off value of <28, resulted in only 1 false positive (3%), whereas RQ, with a cut-off value of >15, produced 6 false positive results (17%). Most of these false positive results normalized in subsequent measurements. The RQ approach detected recurrence in 1 patient 6 months earlier than the raw Cq approach. ConclusionOur preliminary data suggest that this CE-certified assay, using previously suggested cut-off values, is a promising method for detecting disease recurrence, provided a confirmatory second test is conducted to identify false positive results. To avoid unnecessary scans or overtreatment, we are currently validating this assay and cut-offs in a prospective cohort study.

Antibody titer trends after SARS-CoV-2 vaccination in patients aged 12 to 25 years with underlying diseases.

Whereas declines in antibody titers after SARS-CoV-2 vaccination have been reported, most reports are predominantly from adults. Long-term trends in SARS-CoV-2 antibody titers after the first BNT162b2 vaccination series in children and young adults with underlying diseases remain less studied. This prospective single-center observational cohort study enrolled patients aged 12-25 years with underlying diseases who received the first BNT162b2 vaccination series. At least three longitudinal antibody titers were evaluated during the first year following vaccination. Among 429 study subjects, 61 patients with at least three subsequent antibody titer measurements were included. The median (interquartile range [IQR]) time intervals from the vaccination to the first, second, and third antibody measurements were 43 (30-56), 126 (110-155), and 224 (207-256) days, and antibody titers declined with median (IQR) values of 2310 (1440-3515), 2010 (1165-3055), and 1410 (904-2195) U/mL, respectively. In 35 immunocompetent patients, the antibody titers decreased consistently in each measurement. In contrast, the antibody titers in immunocompromised patients remained stable between the first and second measurements, but declined by the third. A two-way ANOVA revealed that time was a more significant factor than immunocompromised status for the declines in antibody titers. In patients aged 12-25 years with underlying diseases, antibody titers in immunocompromised patients after SARS-CoV-2 vaccination were lower than in immunocompetent patients in the early post-vaccination period, but they maintained titers similar to the immunocompetent counterpart during the long-term follow-up.

Comparative evaluation of the upper pharyngeal airway among children with/without UCLP and with/without OSA.

To evaluate the volume and the most constricted cross-sectional area (lumen) sizes of the upper pharyngeal airway among children with/without unilateral cleft lip and palate (UCLP) and with/without obstructive sleep apnea (OSA). This prospective study was conducted on 66 Thai children aged 5 to 12 years, encompassing demographic information, polysomnographic data, and sex distribution: 34 with non-syndromic UCLP (16 with OSA; 18 without OSA) and 32 non-cleft children (16 with OSA; 16 without OSA). Subjects were divided into two age groups: preadolescent group (ages 10-12) and younger group (ages 5-9). Cone-beam computed tomography images were acquired with subjects in a supine position. Subsequent measurements were conducted using the Dolphin imaging program (version 11.7 premium) to investigate and compare the volumes and lumens of the nasopharyngeal, oropharyngeal, and hypopharyngeal airways. In the younger group, the UCLP with OSA group exhibited significantly smaller volumes and lumens in the oropharyngeal airway compared to the non-cleft group without OSA (volume: p = 0.044; lumen: p = 0.031, 95% CI). All upper pharyngeal airway parts had comparable volumes and lumens between age groups. However, preadolescence reported no significant differences. Statistically significant differences were observed only in the oropharyngeal airway measurements in the younger sample compared to the adolescent sample. This underscores the importance of considering oropharyngeal airway structure in diagnosing and preventing OSA in children. However, it is essential to note that while airway size is a fundamental factor, it may not be the sole determinant of OSA occurrence. Other factors likely contribute to the condition as well.

Weakly-Supervised Hair SEM Microscope Image Segmentation Using a Priori Structure Information

The analysis of microscopic images of hair has a wide range of applications in the domains of cosmetics, healthcare and forensics. The segmentation of the hair represents the initial step of automatic large-scale quantitative analysis of microscopic images of hair. It ensures that subsequent quantitative measurements are performed in an appropriate area corresponding to the hair, avoiding artifacts near the boundaries. This process can be time-consuming, tedious and susceptible to subjective errors when conducted by a human operator. Deep learning methods represent a promising solution; however, obtaining pixel-level accurate masks is a costly process. This paper presents a novel weakly supervised pipeline for the segmentation of hair SEM (Scanning Electron Microscope) microscopic images, which requires only simple image-level annotations for training. The proposed method incorporates the Radon transform, the Sobel operator and a novel Boundary Discrimination Module (BD-module) for the estimation of the presence of boundaries. The proposed pipeline was evaluated on a recently collected hair SEM dataset (429 images). Furthermore, it is benchmarked with methods including Unet and Segment Anything Model (SAM). The results demonstrated a mean Hausdorff Distance improvement of over 30% and a standard deviation improvement of over 50% in comparison with the Unet and SAM. Moreover, we proposed additional refinement modules to address boundary nonlinear cases and conducted Grad-CAM analysis to enhance the interpretability of the BD-module. Additionally, we proposed a novel quality estimation metric based on gradient map for self-quality assessment. The SEM hair dataset is accessible to the research community in an open-source format.

Hydrogen Production and Li-Ion Battery Performance with MoS2-SiNWs-SWNTs@ZnONPs Nanocomposites.

This study explores the hydrogen generation potential via water-splitting reactions under UV-vis radiation by using a synergistic assembly of ZnO nanoparticles integrated with MoS2, single-walled carbon nanotubes (SWNTs), and crystalline silicon nanowires (SiNWs) to create the MoS2-SiNWs-SWNTs@ZnONPs nanocomposites. A comparative analysis of MoS2 synthesized through chemical and physical exfoliation methods revealed that the chemically exfoliated MoS2 exhibited superior performance, thereby being selected for all subsequent measurements. The nanostructured materials demonstrated exceptional surface characteristics, with specific surface areas exceeding 300 m2 g-1. Notably, the hydrogen production rate achieved by a composite comprising 5% MoS2, 1.7% SiNWs, and 13.3% SWNTs at an 80% ZnONPs base was approximately 3909 µmol h-1g-1 under 500 nm wavelength radiation, marking a significant improvement of over 40-fold relative to pristine ZnONPs. This enhancement underscores the remarkable photocatalytic efficiency of the composites, maintaining high hydrogen production rates above 1500 µmol h-1g-1 even under radiation wavelengths exceeding 600 nm. Furthermore, the potential of these composites for energy storage and conversion applications, specifically within rechargeable lithium-ion batteries, was investigated. Composites, similar to those utilized for hydrogen production but excluding ZnONPs to address its limited theoretical capacity and electrical conductivity, were developed. The focus was on utilizing MoS2, SiNWs, and SWNTs as anode materials for Li-ion batteries. This strategic combination significantly improved the electronic conductivity and mechanical stability of the composite. Specifically, the composite with 56% MoS2, 24% SiNWs, and 20% SWNTs offered remarkable cyclic performance with high specific capacity values, achieving a complete stability of 1000 mA h g-1 after 100 cycles at 1 A g-1. These results illuminate the dual utility of the composites, not only as innovative catalysts for hydrogen production but also as advanced materials for energy storage technologies, showcasing their potential in contributing to sustainable energy solutions.

Safety evaluation and modulatory effects on innate immune system of pyrazoline-derived compounds.

Pyrazolines are compounds that have been studied for their strong biological potential and structure diversity. Several studies demonstrated their biological effectiveness, highlighting their anti-inflammatory potential. This study aimed to evaluate the physicochemical profile, the safety, and the anti-inflammatory effects of four pyrazolines (PH0, PH3, PH4, and PH7). Initially, in silico analysis were performed on SwissADME and QSAR Toolbox platforms. The anti-inflammatory activity was assessed by in vitro and in vivo methodologies. Neutrophils collected from mice peritoneum and macrophages immortalized cell line (Raw 264.7) were stimulated with lipopolysaccharide (LPS), and subsequent measurement of nitric oxide (NO) and IL-1β, TNF, and IL-6 cytokines were performed by ELISA method. The effect on cell migration was evaluated by chemotaxis assay. The effect on efferocytosis was investigated using senescent neutrophils and macrophages from mice's bone marrow. The in silico results suggest suitable properties for a pharmacological prototype for oral administration, with no significant toxic effects. All compounds significantly reduced NO levels, as well as levels of IL-1β, TNF, and IL-6 cytokines. Also, they were able to reduce cell migration and increase efferocytosis. The in vivo air pouch model confirmed the effects of pyrazolines on cell kinetics and on the levels of cytokines (IL-1β and TNF) on the air pouch lavage. All of the pyrazolines evaluated showed to have positive effects on mechanisms that modulate the inflammatory response. Furthermore, the in silico analysis suggests that chemical changes in the structure can lead to improvement of the biological and pharmacokinetics proprieties.

Densely Populated Cell and Organelles Segmentation With Multi-Plane Deep Learning Pipeline.

Here we develop a robust machine vision pipeline for cell and organelle segmentation within vEM datasets. We collect neural network predictions along multiple planes, capturing 3D correlations using only 2D neural networks. We segment and analyze hundreds of platelets and report quantitative morphological measurements. We apply our segmentation pipeline to the CREMI neuron segmentation challenge data and provide state-of-the-art results. Our work paves the way for large-scale platelet studies and lends unique insight into platelet structure. We publish a lightweight Jupyter notebook for initializing and running the neural network. We also provide Amira Avizo protocols for converting neural network outputs to cell and organelle instances and subsequent morphological measurements.

Abstract 4137672: Prevalence, Clinical Characteristics, and Outcomes in Heart Failure with Improved Ejection Fraction by Sex

Background: Heart failure with improved ejection fraction (HFimpEF) is a growing clinical entity. While males are more likely to present with HFrEF, there remains little understanding of how sex relates to the epidemiology, predictors, and progression of HFimpEF. Research Question: What are the differences in epidemiology, clinical characteristics, medical management, and outcomes in patients (pts) with HFimpEF based on sex. Methods: We identified adults age ≥18 years with incident HFrEF (EF ≤40%) between January 2013 and December 2018 within Kaiser Permanente Northern California, an integrated health system serving &gt;4.5 million members. HFimpEF was defined as subsequent EF measurement &gt;40% within the following 12 months. Sex was based on self-report. Among pts with HFimpEF, we examined guideline-directed medical therapy (GDMT) use at time of incident HFimpEF, and at 6- and 12-months post-HFimpEF, stratified by sex. The incidence of worsening HF (WHF) events and all-cause death was assessed in a landmark analysis at 12 months post-HFrEF. We compared event rates between patients with HFimpEF and persistent HFrEF using Cox proportional hazards models adjusted for baseline age and EF. Results: Among 12,639 patients with incident HFrEF (female: 36%), 33% experienced HFimpEF. Females were more likely to experience HFimpEF in the 12 months following HFrEF than males (34% vs. 32%, p&lt;0.01). Among pts with HFimpEF, treatment with GDMT was similar among males and females and declined after incident HFimpEF in both sexes (Figure, Panel A). Females had similar rates of WHF or all-cause death compared to males (adjusted [a]HR: 1.10, 95% CI: 0.96, 1.26, P=0.17). Compared with those with persistent HFrEF, pts with HFimpEF had lower rates of WHF or all-cause death, a finding which was consistent by sex (male: aHR: 0.55, 95% CI: 0.50 to 0.61, female: aHR: 0.54, 95% CI: 0.48 to 0.62; Pinteraction by sex=0.78; Figure, Panel B). Conclusions: HFimpEF is more common among females compared to males, but prognosis is similar. Despite similar medical therapy, GDMT discontinuation after EF improvement is prevalent. Improvement in EF is similarly associated with improved outcomes across sex. Further data are needed to elucidate the differential etiologies of HF by sex.

Measurement of Steam-Generator-Tube Vibration Damping Caused by Anti-Vibration-Bar Supports

Abstract In 2013, Atomic Energy of Canada Limited (now Canadian Nuclear Laboratories) experimentally measured the damping of a straight tube with a variety of tube supports to examine low-frequency damping. These tests were motivated by the discovery of severe tube damage caused by in-plane fluidelastic instability (FEI) in the U-bend regions of new replacement recirculating steam generators (SG). The measurements were intended to assess the applicability of existing design guidelines for estimating the support-related damping of a tube. In the tests, the damping ratios of a single steam generator tube were measured using both log-decrement and power-based methods. Noncontacting excitation and position-sensing techniques were employed to improve accuracy. Initial baseline tests explored configurations with no support and drilled-hole supports of different hole sizes, and these results were compared with previously published work. Subsequent tests were performed to measure damping of tube vibration parallel to flat-bar supports. Most of the tests were performed with the tube fully submerged in still water. The tests examined the effects of fluid (water or air), natural frequency, gap width, preload, and vibration normal to the bars. This paper describes the test apparatus, methods, and analysis techniques. A summary of the results is presented. The initial baseline results showed that the damping ratios measured without any supports and with a drilled-hole support were consistent with previously published data. However, the subsequent measurements showed that, contrary to a published design guideline, the anti-vibration bars resulted in no significant additional viscous or squeeze-film damping when the vibration was parallel to the bars. On the other hand, the results did show that anti-vibration bars could introduce significant in-plane Coulomb-type damping if there was sufficient tube-to-support preload or impacting.

PRAKTIK JUAL BELI BIBIT LELE DENGAN SISTEM TAKARAN PERSPEKTIF HUKUM ISLAM DAN HUKUM POSITIF

The growing public interest in catfish farming has resulted in an increased demand for catfish fingerlings. This trend has led many individuals to engage in the trade of catfish fingerlings, either as a primary source of income through the establishment of fisheries groups or as a supplementary means of generating additional revenue. This study aims to investigate the implementation of the catfish fingerling trading system based on volume measurement from the perspectives of Islamic law and positive law. Utilizing a qualitative research approach, primary data were collected through interviews and documentation. The primary data sources include informants from fisheries business groups and individuals involved in the trade of catfish fingerlings using the volume-based measurement system. The findings reveal that the volume measurement system is employed to simplify the calculation process, although the initial reference measurement is not consistently aligned with subsequent measurements. Nonetheless, both parties agree to add an additional volume as compensation for potential mortality of the fingerlings. From the perspective of Islamic law, the catfish fingerling trading practices in Gunung Sembung Village, Pegaden Subdistrict, Subang Regency, fulfill the essential elements and conditions required for lawful transactions under Islamic law and are deemed valid according to positive law, as they conform to existing legal regulations.

Evaluation of early cardiotoxicity in HER2-positive breast cancer patients receiving radiotherapy and concurrent trastuzumab.

Overexpression of human epidermal growth factor receptor 2 (HER-2) is associated with aggressive disease in breast cancer. Trastuzumab and radiotherapy are standard treatments for patients with HER-2 + breast cancer, but they may increase the risk of cardiotoxicity. This study aimed to assess early cardiotoxicity in patients receiving radiotherapy (RT) and concurrent trastuzumab. The study included 116 patients with HER-2 + breast cancer who received concurrent treatment with trastuzumab and RT (52 right-side; 64 left-side). Five left ventricular ejection fraction (LVEF) measurements were performed: one before treatment and four subsequent measurements taken at three-month intervals. LVEF was also assessed before (preRT-EF) and after (postRT-EF) radiotherapy. The baseline LVEF was 62.27 ± 5.5%, while the 12-month LVEF was 59.8 ± 5.8% (p < 0.05). In subgroups, post-RT LVEF values ​​were significantly lower than pre-RT LVEF values (p < 0.05). No significant difference was found between the reduction in LVEF for patients receiving 50Gy and 60Gy doses. Moreover, the contribution of regional lymph node irradiation to the decrease in LVEF could not be demonstrated. A positive correlation was found between the total trastuzumab dose and the decrease in LVEF from preRT to postRT. Additionally, a positive correlation was observed between the total taxane dose and the reduction in LVEF from baseline to 9months, both in the overall group and in the left breast cancer group. In our study,it was found that not only trastuzumab but also taxane-based agents could be cardiotoxic. However, no connection was found between RT doses and the decrease in LVEF.

Specific loss of GIPR signaling in GABAergic neurons enhances GLP-1R agonist-induced body weight loss

ObjectivesDual incretin agonists are among the most effective pharmaceutical treatments for obesity and type 2 diabetes to date. Such therapeutics can target two receptors, such as the glucagon-like peptide-1 (GLP-1) receptor and the glucose-dependent insulinotropic polypeptide (GIP) receptor in the case of tirzepatide, to improve glycemia and reduce body weight. Regarding body weight effects, GIPR signaling is thought to involve at least two relevant mechanisms: the enhancement of food intake reduction and the attenuation of aversive effects caused by GLP-1R agonists. Although it is known that dual GLP-1R-GIPR agonism produces greater weight loss than GLP-1R agonism alone, the precise mechanism is unknown. MethodsTo address this question, we used mice lacking GIPR in the whole body, GABAergic neurons, or glutamatergic neurons. These mice were given various combinations of GLP-1R and GIPR agonist drugs with subsequent food intake and conditioned taste aversion measurements. ResultsA GIPR knockout in either the whole body or selectively in inhibitory GABAergic neurons protects against diet-induced obesity, whereas a knockout in excitatory glutamatergic neurons had a negligible effect. Furthermore, we found that GIPR in GABAergic neurons is essential for the enhanced weight loss efficacy of dual incretin agonism, yet, surprisingly, its removal enhances the effect of GLP-1R agonism alone. Finally, GIPR knockout in GABAergic neurons prevents the anti-aversive effects of GIPR agonism. ConclusionsOur findings are consistent with GIPR research at large in that both enhancement and removal of GIPR signaling are metabolically beneficial. Notably, however, our findings suggest that future obesity therapies designed to modulate GIPR signaling, whether by agonism or antagonism, would be best targeted towards GABAergic neurons.

Promoted metabolic remolding by overexpression of AspAT9 ameliorates cadmium toxicity in Arabidopsis

Cadmium (Cd) is a toxic heavy metal that poses a serious threat to crop safety and human health. Aspartate aminotransferase (AspAT) is a prime enzyme engaged in amino acid metabolism, contributing essential metabolic substances for plant growth and acclimatization to various stresses. In this study, we identified a novel AspAT9 gene with high responsiveness to Cd stress from poplar ‘Nanlin895′ and subsequently transformed it into Arabidopsis. The resulting transgenic AspAT9–1 plants (designated A9) and wild-type (WT) Arabidopsis were subjected to Cd treatment (T) or maintained under control conditions (CK). Phenotypic assessments showed that A9 plants displayed greater resistance to Cd stress compared to WT, as evidenced by their stay-green trait and higher biomass. Subsequent metabolism measurement revealed that A9 plants accumulated more Cd in their roots than WT. Meanwhile, the content of various proteinogenic amino acids, Cd-chelating compounds, such as lignin and phytochelatins (PCs), along with antioxidants like glutathione (GSH) and chlorophyll in A9-T exceeded WT-T. Further RNA-seq analysis uncovered significant transcriptional changes in genes implicated in aspartate-glutamate metabolism, antioxidant systems, phenylpropanoid biosynthesis, transporters, and photosynthesis activities. Our findings demonstrate the beneficial effect of overexpressing AspAT9 on Cd phytoextraction, highlights its potential as a valuable genetic resource for phytoremediation applications.

Neighborhood Social and Physical Environments and Sedentary Behaviors: Importance of Early Childhood in Shaping Health Behaviors.

This study aims to investigate the persistence of sedentary behaviors from early childhood to adolescence and the longitudinal association between neighborhood social and physical environments and childhood sedentary behaviors. Using the Future of Families & Child Wellbeing Study (N = 2716), we conducted a cross-lagged path analysis to examine the longitudinal association between neighborhood social and physical environments (neighborhood socioeconomic status, observed physical disorder, and crime rates) and sedentary behaviors at subsequent measurement points, after controlling for demographic factors and family socioeconomic status. We observed the continuity of sedentary behaviors in early childhood, extending throughout adolescence. Neighborhood socioeconomic status was significantly associated with sedentary behaviors at subsequent measurement points from early childhood to adolescence. Observation-based physical disorder and crime rates were associated with engagement in greater sedentary behaviors in adolescence but not in early and middle childhood. We also observed a partial mediation effect of neighborhood physical disorder and crime rates in the association between neighborhood socioeconomic status and sedentary behaviors in adolescence. Our results demonstrated the importance of early childhood in shaping healthy behaviors and highlighted the contribution of unfavorable environments to sedentary behaviors. These findings call for the need to support disadvantaged neighborhoods, particularly by addressing safety concerns and physical disorder, for promoting physical activity and reducing sedentary behaviors among adolescents.

The relationship between sense of coherence and self-efficacy with well-being and mental health-the situation of students at a typical German university during the COVID-19 pandemic and 1 year after the lifting of social restrictions.

The present study aims to evaluate the role of sense of coherence and self-efficacy in relation to mental health and well-being, with and without social restrictions due to the COVID-19 pandemic. Additionally, this study seeks to investigate the differences in sense of coherence, self-efficacy, mental health and well-being depending on the manner in which the pandemic is being handled. A total of 27,162 students at Heidelberg University were surveyed via email at two measurement points, once with and once without social restrictions. The survey assessed sense of coherence, self-efficacy, mental health and well-being. To this end, the questionnaires Sense of Coherence Scale, Patient Health Questionnaire, WHO-Well-being-Index and the General Self-Efficacy Scale were employed. A total of 2,398 individuals participated in the initial measurement, while 701 individuals participated in the subsequent measurement. The lifting of social restrictions has been associated with a notable improvement in well-being and mental health, particularly in the context of depressive syndromes. Further analysis demonstrated a positive correlation between the sense of coherence and self-efficacy at both measurement points, as well as between these two constructs and mental health and well-being. Furthermore, the sense of coherence and self-efficacy were found to account for a notable proportion of the observed variability in mental health and well-being values. Self-efficacy exhibited a significantly higher mean value at the initial measurement time point compared to the subsequent time point. In contrast, no significant difference was observed in the sense of coherence between the two measurement points. The findings presented here illustrate the significance of social interaction, sense of coherence and self-efficacy for mental health and well-being.