Internal brown spot (IBS) is a physiological disorder of potato tubers consisting in the appearance of punctiform and/or enlarged rust-coloured necrosis in parenchymal tissues. The IBS disorder leads to important economic impact and yield depreciation since quality, sensory and processing features of tubers may be highly compromised. Nowadays, the causes of IBS are still debated; however, the combination of several genetic, nutritional and environmental factors seems to have a relevant role in modulation of symptoms. In the current work, IBS occurrences together with skin roughness were monitored in potato tubers of different cultivars harvested in 2 years of field trials under different field conditions (environment, irrigation rate). All three IBS-susceptible cultivars used, Luminella, Majestic and Ricciona di Napoli, showed significant IBS incidence. Importantly, incidence and severity increased with tuber size. Ricciona di Napoli was the cultivar most affected by both IBS and skin roughness. Findings showed a strong positive correlation between IBS and skin roughness, suggesting that suboptimal growth conditions may have affected both physiological disorders. The association between IBS and potato skin roughness may provide new insights for the implementation of non-destructive IBS detection in potatoes.