Chronic consumption of a mixture of conjugated linoleic acid isomers reduces body fat deposition. Acute ingestion apparently enhances metabolic rate and fatty acid oxidation in animal models. Recent research suggests that effects on lipid metabolism are due primarily to the trans-10, cis-12 isomer of CLA (10, 12CLA). PURPOSE To examine the effects of acute oral 10,12CLA on resting and exercise metabolic rate and fuel use in humans. METHODS Thirteen 18–25y, healthy, untrained males underwent baseline VO2peak testing. Fasted subjects consumed 4.8g of 10,12CLA (EXP) or an olive oil placebo on four different occasions (two resting, two submaximal exercise trials for 40 min at 60% VO2peak). For each of these trials, four blood samples (10 subjects) and measures of metabolic rate (indirect calorimetry) and respiratory quotient (RQ) were measured in 10 min segments (prior, 80, 110, and 140 min post supplement ingestion). Exercise began 80 min post ingestion for exercise trials. Blood was analyzed for glucose, glycerol, and fatty acids. RESULTS For resting trials, serum 10,12CLA was significantly higher by 80 min post ingestion (0.38±0.19 μ g/ml) compared to baseline (0.0 μ g/ml) with a peak at 140 min (10.10±2.50 μ g/ml,p < 0.05). Four subjects showed no change in serum 10,12CLA during exercise EXP trials. Those who responded during the exercise trial had a peak in serum 10,12CLA at 110 min (12.83 μ g/ml±3.3, p < 0.05) which decreased to 9.09 μ g/ml ± 3.3 by 140 min. There was no effect of supplement on RQ at rest while there was a trend for a more rapid drop in RQ during exercise in EXP(p = .062). No significant differences between treatments were found for glucose, glycerol, resting or exercise metabolic rate for any time points. CONCLUSIONS At rest, 10,12CLA significantly rose in the blood by 80 min after ingestion of 4.8g. Exercise appeared to interfere with 10,12CLA absorption in some subjects. There was no significant effect of the supplement on indicators of lipolysis, fuel use, or metabolic rate at rest or during exercise.
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