GELIDIUM CARTILAGINEUM is a red alga with repeatedly pinnately branched, maroon-purple fronds. In southern California it is represented by the variety robustum, which grows most abundantly on submarine rocks 10 to 40 or more feet below sea level, and which may reach a length of 4 feet. This plant is the principal agarophyte for the manufacture of agar on the Pacific coast of North America. Agar shortage in the last few years has stimulated considerable interest in the study of agarophytes. The importance of raw materials in an industry cannot be over emphasized. The success of the American agar industry will depend, in large measure, on the adequacy of the supply of agarophytes. A knowledge of their physiology and ecology is prerequisite to finding ways of insuring an adequate natural supply of these seaweeds, or, if necessary, of cultivating them on a commercial scale. A program of research on agarophytes has therefore been inaugurated at the Scripps Institution of Oceanography. The present report, the first of a series, is concerned with the photosynthetic activities of this Gelidium. Photosynthesis is the fundamental process providing both energy and substance for the synthesis of the whole plant organism. It follows that in studies of photosynthesis are to be found solutions to many problems concerning the ecological behavior of plants. It is therefore the object of the authors to study the photosynthetic activity of Gelidium cartilagineum under different environmental conditions, and especially to determine the effect of varying light, temperature, and carbon dioxide concentration in sea water, in order to obtain some idea of the optimum conditions for the growth of this particular plant. THE CARBON DIOXIDE FACTOR.-Since photosynthesis is a process requiring carbon dioxide as a substrate, the rate of photosynthesis cannot be constant over a period of time unless carbon dioxide is supplied at a constant rate, or enough is continually present to saturate the enzyme system involved. It has been well established that carbon dioxide is a limiting factor in the photosynthesis of land plants during the day, since the photosynthetic activitv of leaves is greatly enhanced by increasing the carbon dioxide concentration in the air. In the case of algae and aquatic plants under natural conditions, e.g., filamentous algae and phytoplankton in particular,