Background: Although the comparison between self-managed rest and fixed rest periods in subjects experienced in lower-limb strength training has been investigated, the results remain unclear due to controversies among some studies. Therefore, the present study aimed to analyze the role of self-managed rest versus fixed rest in athletic performance, mean propulsive velocity, velocity loss, muscle oxygen saturation, and rest time in trained subjects; Methods: Thirteen subjects with a minimum of one year of training experience (age (years): 26.31 ± 3.84; height (cm): 175.46 ± 5.61; weight (kg): 79.24 ± 6.83) were randomly assigned to two groups (self-selected rest group [SR] = 7 and fixed rest group [FR] = 6). The subjects underwent a session for evaluation (one maximum repetition (1RM) estimation, familiarization, and data collection) and another day for a traditional strength training session for the back squat, consisting of five sets of four repetitions at 80% of 1RM. One group took a fixed 2 min break, while the other group managed their breaks autonomously (resuming when they felt ready to perform the next set at maximum velocity). Mean propulsive velocity (MPV) was monitored using a linear position transducer, and muscle oxygen saturation (SmO2) was measured with a near-infrared spectroscopy device; Results: Significant differences between the groups were found for the rest time between the first and second sets (SR 97.29 ± 23.70 seg vs. FR 120 ± 0.00 seg). However, no differences were found for MPV, velocity loss, or SmO2; Conclusions: Given the similarities in performance and physiological outcomes between fixed and self-selected rest conditions, both can be used equally depending on the preferences and training goals of coaches and athletes.