To gain optimal positioning to make sure the game laws are applied in uniform way, the performance of field referee must be periodically evaluated to have constantly adequate training during a match and during the competitive season. Considering that field Yo-Yo Intermittent Recovery Test-Level 1 is frequently employed in elite team sport players to estimate maximal oxygen uptake (VO<inf>2max</inf>) in field settings, the aim of this cross-sectional, gender-comparative study was to develop a new adjusted Yo-Yo-1 equation for estimating VO<inf>2</inf>max in football referees. During off-season, 20 male (21.2±3.6 yrs) and 20 female (22.5±4.6 yrs) sub-elite football referees underwent laboratory treadmill test and Yo-Yo-1. VO<inf>2max</inf> during Yo-Yo-1 and laboratory treadmill test were significantly correlated in the whole sample (r=0.871; P<0.0001), women (r=0.861; P<0.0001) and men (r=0.800; P<0.0001). Only in women VO<inf>2max</inf> during Yo-Yo-1 was lower than laboratory treadmill test (-4.3%; P=0.014). Adjusted formulae (pooled and gender-based) allowed to mitigate the underestimated values of laboratory treadmill test of VO<inf>2max</inf> found with the Bangsbo's equation (pooled sample: -14.9%, P<0.0001; women: -13.2%, P<0.0001; men: -16.4%, P<0.0001). Adjusted Yo-Yo-1 equations allow to accurately monitor changes in cardiopulmonary performance, and based on this, administer constantly adequate training loads, in male and female field referees.
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