ABSTRACTHigh-resolution chironomid (Insecta: Diptera) stratigraphies were developed for three subalpine lakes in the Sierra Nevada of California to assess whether these lakes have been impacted by recent climate change evident in regional instrumental records for the 19th and 20th centuries. Detrended correspondence analysis (DCA) of the chironomid fauna indicates that the lakes have experienced similar unidirectional change in community composition over the 20th century, with two of the lakes showing particularly sharp gradients of change since the 1970s. Application of a chironomid-based surface water temperature inference model (r2 jack = 0.73, RMSEPjack = 1.1°C, and a maximum bias of 1.24°C) to the subfossil chironomid assemblages preserved in the lake sediment provided quantitative estimates of surface water temperature changes and revealed the existence of similar water temperature trends between the late 19th century and the present. Above average water temperatures characterized the late 20th century and below average surface water temperatures occurred between a.d. 1910 and a.d. 1980. Fluctuations in the surface water temperature of these lakes closely track changes in mean July air temperature as measured in Fresno, California, over the period a.d. 1895–2001. It appears that 20th century climate change has had an overriding influence on the composition of the chironomid communities within these three lakes. This study demonstrates that subfossil chironomid analysis can provide detailed records of community response to local and regional climatic changes at subdecadal time scales. It also suggests that chironomid communities in subalpine lakes in the Sierra Nevada are already recording the impact of recent climate warming.