The study explores the Ichthyofaunal diversity of freshwater Cyprinidae fish in the Panjkora River of Dir, Khyber Pakhtunkhwa, Pakistan. A total of 911 fish samples were collected from 5 different sites along the river and identified using standard identification keys. Out of the 911 fish samples, 204 were related to family Cyprinidae, having 4 sub families, i.e Cyprininae representing 3 species i.e Carassius auratus, Cyprinus carpio, Cyprinus watsoni, Danioninae representing 6 species i.e Barilius modestus, Barilius vagra, Barilius pakistanicus, Amblypharangodon mola, Salmophasia punjabensis, Rasbora daniconius, Labeoninae representing 5 species i.e Tor putitora, Crossocheilus diplocheilus, Gara gotyla, Labeo rohita, Labeo diplocheilus, and Barbinae representing 6 species i.e Puntius ticto, Puntius sophore, Puntius chonchonius, Schizothorax esocinus, Schizothorax labiatus and Schizothorax plagiostomus. This study adds 6 new species i.e Amblypharangodon mola, Salmophasia punjabensis, Labeo rohita, Labeo diplocheilus, Puntius chonchonius and Rasbora daniconius for the 1st time which are not reported earlier. EXCEL 2019 and XLSTAT were used for principal components analysis to study richness and correlation of fish diversity. Eigenvalues obtained from Sheringal to Zulam are 3.72, 0.511, 0.37, 0.29 and 0.09 respectively. The higher eigenvalue of Sheringal site shows higher diversity. Water quality study shows that the mean values of water temperature was 16.8°C, pH 7.08, total hardness134.6 mg/L, alkalinity 89.3 mg/L, Nitrate 5.93 mg/L, Chlorine 0.87 mg/L, Nitrite0.02 mg/L and Copper 0.04 mg/L, Iron 0.07 mg/L, Lead 0.002 mg/L. The physicochemical parameters of river Panjkora during the study time remained within the permissible limits as prescribed by the WHO (2005). Gut analysis revealed the presence of algae, nymphs insect's larvae, macroinvertebrates and protozoans.
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