The passive bristling mechanism of the scales on the shortfin mako shark (Isurus oxyrinchus) is hypothesized to play a crucial role in controlling flow separation. In the hypothesized mechanism, the scales are triggered in response to patches of reversed flow at the onset of separation occurring in the low-speed streaks that form in a turbulent boundary layer. The two goals of this investigation were as follows: (1) to measure the reversing flow occurring within the low-speed streaks in a separating turbulent boundary layer; (2) to understand the passive flow control mechanism of movable shark skin scales that inhibit reversing flow within the low-speed streaks. Experiments were conducted using digital particle image velocimetry (DPIV). DPIV was used to analyze the flow in a turbulent boundary layer subjected to an adverse pressure gradient formation over both a smooth flat plate and a flat plate on which shark skin specimens were affixed. The experimental analysis of the flow over the smooth flat plate corroborated the findings of previous direct numerical simulation studies, which indicated that the average spanwise spacing of the low-speed streaks increases in the presence of adverse pressure gradients upstream of the point of separation. However, the characteristics of the flow over the shark skin specimen more closely resemble that of a zero-pressure gradient turbulent boundary layer. A comparative analysis of the width and velocity of the reversed streaks between flat plate and shark skin cases reveals that the mean spanwise spacing decreases, and thus, the number of streaks increases over the shark skin. Additionally, the reversed streaks observed over shark scales are thinner and the highest negative velocity within the streaks falls within the range required to bristle the scales.