Correlational Study Research Articles

Characteristics and Correlation Study of Mountainous Lake Ice Phenology Changes in Xinjiang, China Based on Passive Microwave Remote Sensing Data

Lake ice phenology directly reflects local climate changes, serving as a key indicator of climate change. In today’s rapidly evolving climate, utilizing advanced remote sensing techniques to quickly extract long-term lake ice phenology features and studying their correlation with other climate factors is crucial. This study focuses on lakes in Xinjiang, China, with a mountainous area greater than 100 km2, including Sayram Lake, Ayahkum Lake, Achihkul Lake, Jingyu Lake, and Ahsaykan Lake. The Bayesian ensemble change detection algorithm was employed to extract lake ice phenology information, and the Mann–Kendall (MK) non-parametric test was used to analyze trends. The visual interpretation method was used to interpret the spatial evolution characteristics of lake ice, and the Pearson correlation coefficient was used to explore the driving factors of lake ice phenology. Results indicate the following: (1) Jingyu Lake exhibited the most significant delay in both freezing and complete freezing days, while Ayahkum Lake showed the most stable pattern. Ahsaykan Lake demonstrated the least delay in both starting and complete melting days. (2) Sayram Lake’s ice evolution was unstable, with wind causing variability in the locations where freezing begins and melting spreading from the west shore. Ayahkum Lake, Ahsaykan Lake, and Jingyu Lake exhibited similar seasonal variations, while Achihkul Lake’s ice spatial changes spread from the east to the center during freezing and from the center to the shore during melting. (3) The study found that the freeze–thaw process is influenced by a combination of factors including lake area, precipitation, wind speed, and temperature.

The gut microbiota metabolite trimethylamine-N-oxide in children with β-thalassemia: potential implication for iron-induced renal tubular dysfunction.

Renal tubular dysfunction is common in transfusion-dependent β thalassemia (β-TM). Iron overload, chronic anemia, and hypoxia are precipitating factors for renal insult. However, gut microbiota engagement in the renal insult has not been explored. Our work aimed to assess the potential link between iron overload, gut leakage/dysbiosis, and kidney dysfunction in these children. We enrolled 40 children with β-TM and 40 healthy controls. Gut leakage/dysbiosis biomarkers (trimethylamine-N-oxide [TMAO] and fecal short-chain fatty acids [SCFAs]), oxidative stress and inflammatory biomarkers, TMAO-regulated proteins such as serum sirtuin 1 (S.SIRT1) and serum high mobility box group-1 (S.HMGB1), and tubular dysfunction biomarkers were assessed. Correlations and regression analysis were performed to assess the relation between different parameters. Iron overload, redox imbalance, and generalized inflammation were evident in children with β-TM. Renal tubular dysfunction biomarkers and S.TMAO were significantly elevated in the patient group. Furthermore, fecal SCFAs were significantly lower with upregulation of the investigated genes in the patient group. The correlation studies affirmed the close relationship between circulating ferritin, TMAO, and renal dysfunction and strongly implicated SIRT1/HMGB1 axis in TMAO action. Gut dysbiosis may have a role in the pathogenesis of renal injury in children with β-TM. Renal tubular dysfunction is a prominent health issue in β thalassemia major (β-TM). Iron overload, chronic anemia, and hypoxia are known precipitating factors. However, gut microbiota engagement in renal insult in these patients has not yet been explored. We aimed to assess potential link between iron overload, gut leakage/dysbiosis, and kidney dysfunction in β-TM children and to highlight the SIRT1/HMGB1 axis, a signal motivated by the gut microbiota-dependent metabolite trimethylamine-N-oxide (TMAO), involvement in such insults. We found that gut leakage/dysbiosis may have a role in kidney dysfunction in β-TM children by exacerbating the iron-motivated oxidative stress, inflammation, ferroptosis, and modulating SIRT1/HMGB1 axis.

Releasing our model T - chimeric antigen receptor (CAR) T-cells for autoimmune indications.

This review provides an update on the rapidly growing field of engineered cellular therapies for autoimmune disorders, primarily focusing on clinical experience and correlative studies with chimeric antigen receptor (CAR) T-cells. To date, two case series describing treatment with CAR T-cell therapy for systemic lupus erythematosus (SLE) suggest that drug-free remission can be sustained in patients with previously treatment-refractory disease. The outcomes of these studies are similar, despite the use of different CAR constructs and lymphodepletion regimens. Although it is not yet clear whether the patients described have truly been cured, the majority of remissions have remained durable up to last follow-up at 1-2 years from treatment. Meanwhile, mechanistic studies are providing a window into how transient B-cell depletion mediates lasting benefit. With the encouraging data in SLE, CAR T-cells and other novel B-cell-depleting agents (e.g. bispecific T-cell engagers) are now being evaluated as treatment for other autoimmune conditions, with the goal of durable response. Recent reports highlight cellular therapies as a promising strategy for patients with treatment-refractory autoimmune conditions; however, there is still limited experience, and better insight into this therapeutic approach is expected to emerge rapidly.

Experience of Labour and Childbirth in a Sample of Portuguese Women: A Cross-Sectional Study

Background/Objectives: Childbirth is a profoundly personal experience that often does not align with expectations. The World Health Organization has established guidelines for best practises; in this sense, it is crucial to understand the childbirth experiences of Portuguese women in comparison with these guidelines. Methods: A quantitative, descriptive, correlational, and cross-sectional study was conducted to achieve this. In total, 615 women completed a sociodemographic questionnaire and the Labour and Childbirth Experience questionnaire, which comprised 39 statements based on the WHO’s recommendations. Additionally, the study utilized the Life Satisfaction Scale and gathered insights into participants’ overall perception of care during this phase. Results: The results were categorized as follows: (1) practises influencing the labour experience; (2) practises influencing the experience of vaginal birth; (3) practises affecting the experience of caesarean birth; and (4) emotional experience during labour and birth. Conclusions: Notably, the study found that practises discouraged by the WHO are still prevalent, potentially enabling obstetric violence. However, a robust and statistically significant correlation was observed between the childbirth experience and the overall perception of care.

Prevalence of Dunning-Kruger effect in first semester medical students: a correlational study of self-assessment and actual academic performance

IntroductionThe ability to self-assess is a crucial skill in identifying one’s own strengths and weaknesses and in coordinating self-directed learning. The Dunning-Kruger effect occurs when limited knowledge causes individuals to overestimate their competence and underestimate others’, leading to poor self-assessment and unrecognized incompetence. To serve as a foundation for developing strategies to improve self-assessment, the self-assessment abilities of first-semester students were assessed.MethodsIn the final weeks of the summer 2021, winter 2021/22, and summer 2022 semesters, the academic performance (oral anatomy exam) of first semester students was assessed (0–15 points). Before the exam results were announced, students were asked to self-assess their performance.ResultsExam scores (M = 10.64, SD = 2.95) and self-assessed scores (M = 10.38, SD = 2.54) were comparable. The absolute difference between them, as a measure of self-assessment ability ranged from − 9 to + 9 points (M = -0.26, SD = 2.59). Among participants (N = 426), 18.5% assessed themselves accurately, 35.5% overestimated, and 46.0% underestimated their performance. The correlation between actual score and self-assessment was ρ = -0.590 (p < 0.001), reflecting the Dunning-Kruger effect. When separated by gender, correlation for females was ρ = -0.591 (p < 0.001), and for males ρ = -0.580 (p < 0.001).ConclusionsRealistic self-assessment is a challenge for first-semester students. The data indicate that females tend to overestimate their performance while males underestimate theirs. A pronounced Dunning-Kruger effect is evident in both genders, with significant negative correlations between self-assessment and actual performance. There are several reasons for the occurrence of the Dunning-Kruger effect. Considering that the COVID-19 pandemic influenced learning environments, collaborative learning was significantly restricted. The lack of opportunities for comparison could potentially lead to unrealistic self-assessment.

Evaluation of the psychometric properties of the Turkish version of the Skin-to-Skin Contact Scale for nurses.

Skin-to-skin contact is the practice with the highest level of evidence that increases the growth, development, and healing rate of the newborn. Neonatal nurses are also the best practitioners of skin-to-skin contact. This study examined the psychometric properties of the Turkish version of the Skin-to-Skin Contact Scale for nurses. This descriptive, correlational, and cross-sectional study was carried out with 226 neonatal and pediatric nurses between July 2022 and July 2023. The data were collected using an online questionnaire, including a descriptive information form and a skin-to-skin contact scale for nurses. Data were analyzed by explanatory and confirmatory factor analysis, Cronbach's alpha, and item-total score analysis. It was determined that the scale consisted of 20 items and four sub-dimensions and that those four sub-dimensions explained 53.01% of the total variance. All factor loads were higher than 0.30 in the explanatory and confirmatory factor analyses. All the fit indices were higher than 0.80, and the Root Mean Square Error of Approximation (RMSEA) was less than 0.080 in the confirmatory factor analysis. Cronbach's alpha was determined to be 0.89 for the total scale and greater than 0.70 for all sub-dimensions. The Skin-to-Skin Contact Scale for nurses was determined to be a valid and reliable measurement tool for Turkish nurses. It is recommended that the scale be used regularly in clinics to determine nurses' attitudes towards skin-to-skin contact.

Caregiver burden, religious coping, and spiritual well-being among caregivers of elderly stroke patients and predictors affecting spiritual well-being.

Stroke is a disease that affects the arteries leading to and within the brain. Stroke usually creates a range of disabilities such as physical deficits and difficulty in performing daily activities. These long-term needs and the continuous caregiving sequence affect the care burden and spiritual well-being of caregivers. The correlational study population consisted of caregivers of stroke older patients (N = 100). Burden Interview Scale, Spiritual Well-Being Scale and Religious Coping Scale were used. Descriptive analyses, independent sample t-test, one-way analysis of variance, correlation, regression analyses were used to evaluate the data. The mean age of caregivers was 48.38 ± 13.52 years. The score of Burden Interview Scale was 31.41 ± 15.13 (light/moderate), Negative Religious Coping was 7.39 ± 3.17 (moderate), Positive Religious Coping was 24.30 ± 4.34 (high), and Spiritual Well-Being was 21.65 ± 6.73 (low). There is a significant relationship between Burden Interview and Negative Religious Coping (r = 0.274; P = 0.006), Burden Interview and spiritual well-being (r = -0.563; P = 0.000), and spiritual well-being and positive religious coping (r = 0.228; P = 0.026). The predictors that affect the spiritual well-being of caregivers were found to be Burden Interview (β = -0.571) and Positive Religious Coping (β = 0.181) (P < 0.05). Nurses play an important role in increasing the spiritual well-being levels and religious coping behaviours of caregivers. Nurses should communicate empathically with caregivers, understand their feelings, and provide emotional support.

Investigation of immunosuppressive treatment compliance, dyspnea, anxiety, and depression levels in lung transplant recipients: online interview

IntroductionFollowing lung transplantation (LTx), it is important for recipients to comply with immunosuppressive treatment and cope with related problems. In the post-LTx period, the course of dyspnea and psychological problems it causes in case of progression are not known. Depression and anxiety may develop in recipients after LTx. However, the relationship between this situation and treatment compliance and dyspnea is uncertain.ObjectiveThe aim of this study was to investigate dyspnea, anxiety, and depression levels of recipients following LTx and their immunosuppressive treatment compliance.MethodThe study was planned as a descriptive, correlational, and cross-sectional study. Data were collected on various social media platforms via an online interview, and 65 LTx recipients were included in the sample (n = 65). A Participant Information Form, the Modified Borg Scale, the Beck Anxiety Inventory, the Beck Depression Inventory, and the Immunosuppressive Drug Compliance Scale were employed to collect data. The collected data were analyzed using descriptive statistics, the Mann–Whitney U test, and the Kruskal-Wallis test.ResultsThe mean age of the participants was found to be 52.60 ± 9.44 years, and 56.9% were male. Forty percent of the participants were dependent on oxygen support, and 32.2% had hypertension. Their dyspnea levels were mild, anxiety levels were moderate, depression levels were high, and immunosuppressive treatment compliance levels were slightly above-average. According to the correlation analysis results, dyspnea was associated with anxiety and depression (p &amp;lt; 0.05). As depression increased, immunosuppressive treatment compliance decreased, and the correlation between the two variables was statistically significant (p &amp;lt; 0.05).ConclusionIn this study, as the severity of dyspnea experienced by LTRs increased, the severity of their anxiety and depression also increased. Additionally, there was an inverse correlation between depression and immunosuppressive treatment compliance. LTRs demonstrated insufficient adherence to their immunosuppressive drug regimens, which is very significant in terms of graft survivability. These results suggest that LTRs should be closely monitored at home.

In situ digital image correlation study on the mechanical properties of GH4169 at different temperatures

AbstractGH4169 is a precipitation‐strengthened nickel‐based high‐temperature alloy, and its mechanical behavior at different temperatures is of significant importance for practical applications. Here, an in situ study on the mechanical properties and microstructural evolution of the GH4169 alloy at different temperatures was conducted using scanning electron microscopy in combination with digital image correlation. The results demonstrate the existence of a linear relationship between the local average strain and the macroscopic strain. At temperatures of 20 °C, 100 °C, 180 °C, and 260 °C, the local average strain is 1.96, 2.13, 2.26, and 2.30 times the macroscopic strain, respectively, which shows a clear trend. This indicates that the temperature has a significant influence on the plasticity of the alloy. Additionally, the strain is concentrated below the notch, whereby at a macroscopic strain of 1.7 % at 20 °C and 260 °C, the local maximum strain at the notch is 52 % and 83 %, respectively. Furthermore, when the macroscopic strain reaches 2.12 %, the local maximum strain at the notch is 72 % and 103 %, respectively. At this point, cracks start to initiate and gradually propagate. In situ observations show that at the beginning of the tensile plasticity stage, the plastic strain rate in the grains is twice the rate at the grain boundaries; subsequently, both rates tend to stabilize, and a high‐strain zone appears inside the grains and is coordinately transferred to the surrounding grains through the grain boundaries. The final stage of damage of the alloy consists of perforation fracture, and the presence of a large number of cracked carbides at the fracture results in specimen cracking.

The Effect of Depression and Hopelessness Level on Death Anxiety in Earthquake Survivor Students One Year After the Earthquake.

It is important to reveal the long-term psychological effects of the earthquake in earthquake-exposed individuals. The aim of this study was to determine the levels of depression, hopelessness and death anxiety in students who experienced the earthquake one year after the earthquake and to examine the effects of depression and hopelessness on death anxiety. This is a descriptive and correlational study. Data were collected from 155 Emergency Relief and Disaster Management students who experienced the Kahramanmaras earthquake in Türkiye in 2023. Data were collected using Personal Information Form, Beck Depression Scale, Beck Hopelessness Scale, Thorson-Powell Death Anxiety Scale. In the study, a moderate positive correlation was found, indicating that as levels of depression and hopelessness increased, so did death anxiety (p < .05). Depression and hopelessness explained 18.1% of the variance in death anxiety. Death anxiety was found to be higher in males and in students whose relatives or close friends died.

Personality Traits and Common Ingroup Identity: Support for Refugee Policies Among Host Members

Türkiye, which has hosted the largest number of refugees in recent years, requires remedial intervention programs to facilitate adaptation and coexistence. The irony of harmony studies that guide these interventions seem incomplete due to limited sample characteristics and a lack of attention to personality traits. Hence, we aimed to explore relationships between personality traits, identification with common ingroup identity, and support for social policies toward refugees by sampling the advantaged majority and the disadvantaged largest minority in Türkiye across two correlational studies (Ntotal = 772). In Study 1, agreeableness, extraversion, openness, narcissism, and psychopathy were associated with support for positive social policies. On the other hand, neuroticism was linked with support for negative social policies. However, when identification with common ingroup identity was included in the model, the significance of personality traits in almost all models disappeared, indicating that only the prediction of identification with common ingroup identity remained. The findings of Study 2 replicated and extended the previous result by sampling disadvantaged group members. We suggest that it may be more effective to focus on intergroup variables rather than personality traits to strengthen support for refugee policies, as the overall findings pointed out.

The association between caregiver burden, social capital, and family functioning among caregivers of older adults with PSD - A cross-sectional study.

This study aimed to explore the relationships between social capital, family functioning, and caregiver burden in caregivers of older adults with post-stroke dementia (PSD). A cross-sectional correlational study was conducted between November 2017 and April 2018 among 105 caregivers of older adults with PSD using a demographic questionnaire, the Social Capital Scale, Family APGAR Index (APGAR), and Caregiver Burden Inventory (CBI).An independent samples t-test was also utilized to compare caregiver burden based on demographic characteristics. Statistical analysis comprised Pearson correlation analysis and multiple regression analysis using SPSS version 22.0 to identify influencing factors. Significant negative correlations were found between caregiver burden and variables such as social capital (p < .001) and family functioning (p < .01). The results of the multivariate analysis revealed that social capital, physical condition, and duration of caregiving were the main influencing factors (β = 58.162, ΔR2 = 0.289, p < .001). These findings suggest that nurses should continue to prioritize the well-being of patients' families, and collaborative efforts should be made to establish care facilities that cater to both medical and social needs.

Father-child attachment on children’s screen time: mediating role of ego resilience

BackgroundChildren spend a significant amount of time engaging in sedentary behaviors, defined as activities that do not increase energy expenditure significantly above resting levels, such as watching TV and playing PC/video games. Increased screen time is a significant public health concern because children are in a developmental stage where lifestyle behaviors predict various health outcomes in adulthood and beyond. This study explores the links between parent-child attachment, ego resilience, and children’s screen time.MethodsThis cross-sectional correlation study uses 1,163 parents and their children data from the 12th Panel Survey of Korean Children. The study instruments were a modified Armsden and Greenberg scale of parent-child attachment and modified the children’s ego resilience scale of the Block and Kremen. Children’s screen time was classified as more than 2 h per day spent watching television, playing on computers, or playing video games in their leisure time.ResultsStudy results show a negative association between father-child attachment and children’s screen time (ß = -0.43, p < .001) and a positive association between father-child attachment and ego resilience (ß = 0.24, p < .001). Ego resilience showed a negative link to children’s screen time after adjusting for parental attachment (ß =- 0.03, p = .009). Father-child attachment link to children’s screen time (ß = -0.36, p < .001) continued even though the association was slightly reduced in magnitude after adjusting for ego resilience.ConclusionsOur findings show the mediating role of ego resilience on a negative path of father-child attachment to children’s screen time. This finding emphasizes the importance of fostering solid father-child relationships and enhancing children’s psychological resilience as critical strategies for reducing excessive screen time. By addressing emotional support and resilience-building, interventions can more effectively promote healthier behavioral outcomes in children.

Shaping The Future of Work Readiness of Internship Students : Role of Gen Z’s Resilience

This study aims to analyze the role of resilience on work readiness in MBKM (Merdeka Belajar Kampus Merdeka) internship students. Non-experimental correlational study design was used. A quantitative approach through regression and descriptive statistical analysis was used to analyze the collected data. Non-probability sampling, specifically the convenience sampling, was also used. Data collection was carried out through an online questionnaire using Google Forms. The respondents consisted of 140 students from a university in West Java who had participated in the MBKM internship program. The Resilience Quotient Test (RQ-Test) and Work Readiness Scale (WRS) were used to measure resilience and work readiness, respectively. The result of this study showed that resilience had a significant role on work readiness (r2 = 0.285; p = 0.000). This study is expected to present information and contribute theoretically towards resilience and its role towards work readiness in MBKM internship students. The result of this study can be used by students that participate in the internship program to evaluate themselves and to show them the importance of developing resilience for their work readiness in the future.

Factors Affecting Delirium Care Core Competency Among Nurses: A Descriptive Correlational Study.

Nurses require optimal competency to deliver safe and high-quality care to patients with delirium. However, the factors influencing delirium care core competency remain unclear. This study aims to investigate the factors influencing core competency in delirium care among nurses. This study was conducted as a descriptive correlational study following the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. A convenience sample of 202 Korean nurses from 15 hospitals completed a structured, web-based, self-administered questionnaire. The survey covered general characteristics, delirium care knowledge, moral sensitivity, barriers to delirium care, and core competency in delirium care. Multiple regression analysis identified the factors affecting core competency in delirium care. Results showed that barriers to delirium care, moral sensitivity, academic qualifications, and clinical ladder level were significant factors, accounting for 18.1% of the variance in delirium care core competency. To improve delirium care among nurses, using individual, team, and organizational approaches to address perceived barriers and moral sensitivity is crucial. Competency-based training programs and administrative support are crucial for enhancing the competency of nurses who are novices and those with associate degrees.

Heritability, signal perception and autoregulation of root nodulation in chickpea

Chickpea (Cicer arietinum L.) establishes symbiotic interactions with Mesorhizobium to develop root nodules where nitrogen fixation occurs. This symbiotic relationship can fix atmospheric nitrogen (N2) up to 140 kg N/ha that contribute nearly 80% nitrogen requirement of the crop. Global researchers had revealed the existence of natural variations in chickpea germplasm for nodulation traits with high heritability. Surprisingly, the contribution of environmental variation is too low for Biological Nitrogen Fixation (BNF) traits and high broad-sense heritability (&gt;60) was observed for early nodulation, late nodule senescence and high nodule number traits. Correlation studies indicated a strong positive correlation between nodule number at flowering stage with total nodule weight and plant biomass and seed protein content. Nod Factor receptors in chickpea (CaNFR1 and CaNFR5) are characterized recently that forms phylogenetically distinct group along with M. truncatula, P. sativum, and L. japonicus. Critical role of cytokinin signalling through members of two component system (TCS) in nodulation was investigated in chickpea. The chickpea ortholog CaHK19 was the master spigot of cytokinin perception in chickpea. The co-expression pattern of CaHKs and CaNIN clearly indicated a link between cytokinin perception and downstream expression of CaNIN in chickpea as earlier established in Medicago. Genes involved in AON pathway are partially revealed in chickpea. CaRND1, CaRDN2, and CaRDN3 (C. arietinum Root-Determined Nodulation) function as receptors for signals produced from the roots. Revealing the molecular basis of root nodule organogenesis and their regulatory mechanisms along with identification of potential genetic stock will help on breeding or engineering chickpea genotypes with high symbiotic efficiency, extended nitrogen fixation and high symbiotic efficiency make grain legumes as nitrogen fixing factories to fertilize the soil in a sustainable way.

Assessment of ICU nurses' competency towards delirium among critically ill patients.

ICU nurses play a critical role in managing delirium in critically ill patients, yet their competency in this area remains under-explored. To assess ICU nurses' competency including their knowledge, practice, and attitudes toward delirium among critically ill patients. A correlational descriptive study was conducted with 67 nurses in the medical intensive care unit at Suez Canal University Hospitals. Data was collected using an online survey and analyzed using descriptive statistics, ANOVA, and Pearson's correlation. Nurses showed a moderate knowledge level (M = 8.55), a low practice level (M = 6.62), and positive attitudes (M = 21.65) toward ICU delirium. ANOVA results indicated significant differences in practice scores based on educational level, F (2, 64) = 3.361, p = .041, and in knowledge scores based on ICU experience, F(3, 63) = 6.455, p < .001. Nurses with Master of Science in Nursing (MSN) degrees had higher practice scores than those with diplomas or Bachelor of Science in Nursing (BSN) degrees. There were no significant differences in knowledge and attitude scores based on educational level or age. Gender differences were minimal, with male nurses slightly outperforming female nurses. Correlation analysis showed positive relationships between age, education, ICU experience, and ICU nurses' levels of knowledge and practice. ICU nurses demonstrate a knowledge-practice gap in delirium management. Targeted educational interventions, mentorship programs, and a focus on practical skills are essential to enhance delirium care. These findings highlight the urgent need for comprehensive delirium education and training programs for ICU nurses. By improving nurses' knowledge and practice, healthcare organizations can significantly enhance the early detection, prevention, and management of delirium, ultimately leading to improved patient outcomes and reduced length of stay in the ICU.

Perspective of Midwives in Turkey Regarding Sexual Health Literacy and Sexual Healthcare Services.

This study aimed to examine the factors associated with the level of sexual health literacy of midwives and their views on sexual healthcare. This is a descriptive and correlational study. A total of 324 midwives with at least 1 year of professional experience and at least a bachelor's degree participated in the study. Data were collected with the "Midwife Information Form" and "Sexual Health Literacy Scale." The professional work experience, marital status, and education related to sexual health did not affect the sexual health literacy of midwives. Age, educational status, and voluntary choice of the profession willingly affected sexual health literacy. The sexual health literacy of midwives who hold positive views on sexuality and sexual healthcare services is higher. The midwives' perspectives on sexual health, their level of knowledge, and the sexual health education they receive impact the quality of care they provide. Sexual and reproductive health should be emphasized within the scope of midwifery education.

Clotrimazole-induced shifts in vaginal bacteriome and lipid metabolism: insights into recovery mechanisms in vulvovaginal candidiasis.

Vulvovaginal candidiasis (VVC) is a prevalent condition affecting a significant proportion of women worldwide, with recurrent episodes leading to detrimental effects on quality of life. While treatment with clotrimazole is common, the specific alterations it evokes in the vaginal bacteriome and metabolome were previously underexplored. In this prospective study, we enrolled reproductive-age women diagnosed with single VVC and conducted comprehensive analyses of vaginal fungi, bacteriome, and metabolome before and after local clotrimazole treatment. We observed a significant reduction in Candida albicans and notable improvements in vaginal cleanliness. Advanced sequencing revealed substantial shifts in the vaginal bacteriome, with an increase in Lactobacillus-dominant communities post-treatment. Our findings identified 17 differentially abundant bacterial species, including notable decreases in pathogenic anaerobes such as Gardnerella vaginalis, Dialister micraerophilus, and Aerococcus christensenii, suggesting a restoration of a healthier microbial balance. Furthermore, metabolomic analysis revealed significant changes in 230 metabolites, particularly within lipid metabolism pathways, with marked downregulation of lipid-related compounds linked to inflammation. Correlation studies indicated a strong interplay between lipid metabolites and specific bacterial species, emphasizing the influence of clotrimazole treatment on microbial and metabolic interactions. Importantly, predictive models using microbiota and metabolite signatures demonstrated high accuracy in distinguishing pre- and post-treatment states. This research highlights clotrimazole's dual role in effectively clearing Candida infection and promoting a healthier vaginal microenvironment, paving the way for novel microbial and metabolomic-based diagnostic approaches to enhance VVC management and understand its underlying mechanisms.

The Impact of Self-Directed Learning on Student Motivation and Creativity in Electronics Education: A Correlational Study at SMK Negeri 1 Batipuh

The Impact of Self-Directed Learning on Student Motivation and Creativity in Electronics Education: A Correlational Study at SMK Negeri 1 Batipuh