Educational status of the married women and their conception about pre-natal and post natal care of infants in rural and urban Bangladesh differ from each other to a significant scale. This study was conduced on married women aged from 16 years to 35 years who had at least one child bearing the age below one year. The study area of this research was Khulna city ward no. 24 (considered as the urban study area) and Sonka village of Kaliganj upazila in Satkhira (considered as the rural study area) in Bangladesh. The objective of the study was to understand the educational status of mother and their concept about pre-natal and post-natal care in rural and urban Bangladesh. The study is an explanatory research based where survey research design was used mainly. A total of 100 respondents were selected equally from both urban and rural area following purposive sampling method. It was found from the study that 56 percent of the rural mothers and 36 percent of the urban mothers had education up to primary level (equal to 5 Grade). Findings also reveal that 84 percent of the rural mothers and 48 percent of the urban mothers had rest hours below one to a day. The findings suggest that education had a significant influence over the conception of mothers regarding pre-natal and postnatal care of the infants with a significant variance in the rural and urban areas.