There is no common concept regarding the effectiveness of the use of fluorescence-guided surgery in patients with tumors located in eloquent areas of the brain, in which resection can be stopped because the high risk of an increase of neurological deficit. Objective is to evaluate the effectiveness of fluorescence-guided surgery in patients with tumors located near the motor cortex and corticospinal tract. Methods. Our research includes 108 adult patients with gliomas near the motor cortex and corticospinal tract divided into two groups depending on the use of fluorescence-guided surgery: control (34 patients without fluorescence) and main (62 patients with fluorescence). Results. There is no difference between study groups in the radicality of surgery and neurological outcomes. Conclusion. Fluorescence-guided surgery can be a useful tool as part of complex intraoperative monitoring in patients with tumors located near the motor cortex and corticospinal tract despite the absence of a statistically significant difference.