Study Of Genetic Material Research Articles

A comprehensive exploration of restriction enzymes and their applications in molecular biology: A review

Restriction enzymes, derived from bacteria, serve as indispensable tools in molecular biology, enabling precise manipulation of DNA with specificity. These enzymes recognize specific DNA sequences, cleaving the DNA strand at those designated sites. Two types, blunt-end cutters, and sticky-end producers, offer distinct advantages and disadvantages. In molecular biology, restriction enzymes find diverse applications, prominently in gene cloning, facilitating the insertion of foreign DNA into host organisms. Additionally, they play a vital role in DNA sequencing, DNA fingerprinting, and other techniques crucial for studying gene function and regulation. A groundbreaking development is witnessed in gene editing, where engineered enzymes recognize specific DNA sequences, allowing scientists to target and modify genes with unprecedented precision. This breakthrough holds the potential to revolutionize medicine, paving the way for treating genetic diseases and creating personalized therapies. Research efforts are focused on discovering new restriction enzymes with novel specificities, expanding the range of manipulable DNA sequences. This opens avenues for innovative applications in synthetic biology and biotechnology, further advancing the field. Despite their numerous applications, challenges persist, including the potential for off-target effects and the quest for enzymes with specific recognition sequences. Ongoing research and development continue to push the boundaries of what is achievable with restriction enzymes. In conclusion, restriction enzymes have significantly impacted molecular biology and biotechnology, facilitating the precise manipulation and study of genetic material. Ongoing research promises to unveil new applications and discoveries in this dynamic and promising field.

Изследване на морфологични и качествени показатели на нови линии ориенталски тютюн от екотип Крумовград

In recent years, oriental tobacco varieties from the Krumovgrad ecotype have been among the most preferred by commercial companies in Bulgaria. The creating and study of genetic material with their participation is important to meet the needs and requirements of the sector. During a three-year period in the conditions of experimental field (EF) - Markovo were studied biometric (number of leaves, plant height, length and width of 14 and 21 leaves) and quality (yield and distribution by classes) indicators of four lines of oriental tobacco, created with the Krumovgrad ecotype participation. As control was used variety Krumovgrad 90. As a result of the studied biometric and qualitative indicators, it was established, that according to the indicators plant height, the studied lines are in the oriental tobacco range, but form a greater number of leaves from the control variety (the largest numbers of leaves and smaller by size in line 72). Line 71 has a statistically proven longest length on the 14th and 21st leaves. Lines 71 and 73 have the highest average yield (kg/da) for the three years and exceed the yield of the control variety by 10-15%. The class distribution results show, that lines 72 and 73 have the highest percentage of I-st class, and line 71 has the highest percentage of II-nd class and the lowest percentage of III- rd class. From the assessment made lines 71 and 73 have the best values of the biometric and quality indicators.

Ancient DNA and paleogenetics: risks and potentiality.

SummaryPaleopathology, the science that studies the diseases of the past, has always been addressed to the future in the use of new diagnostic methods. One of its relatively recent branches is paleogenetics, which is the study of genetic material from the past. Nuclear and mitochondrial DNA recovered from archaeological and paleontological specimens is called ancient DNA (aDNA), which can be extracted from a large variety of biological materials, of different origin, state of preservation and age, such as bones, teeth, coprolites, mummified tissues and hairs. There are many applications for ancient DNA research in the field of archaeology and paleopathology: population demography, genealogy, disease studies, archaeological reconstruction of plant vegetation, calibration of the molecular clock, phylogenetic relationship between different mammals and interpretation of the paleoclimate. However, the study of ancient genetic material is extremely difficult due to its poor quality and quantity, as well possible contamination with modern DNA. New advanced methods will allow extracting DNA from a greater variety of materials, and improvements in sequencing techniques will unveil data that are currently concealed.The aim of this paper is to provide initial insights into paleogenetics and ancient DNA study and to illustrate the limits, risks and potentiality of the research on the genetic material of ancient specimens, whose results have a strong impact on the present and future medicine.

Next Generation High Throughput Sequencing to Assess Microbial Communities: An Application Based on Water Quality.

Traditional techniques to identify different contaminants (biological or chemical) in the waters are slow, laborious, and can require specialized expertise. Hence, the rapid determination of water quality using more sensitive and reliable metagenomic based approaches attains special importance. Metagenomics deals with the study of genetic material that is recovered from microbial communities present in environmental samples. In traditional techniques cultivation-based methodologieswere used to describe the diversity of microorganisms in environmental samples. It has failed to function as a robust marker because of limited taxonomic and phylogenetic implications. In this backdrop, high-throughput DNA sequencing approaches have proven very powerful in microbial source tracking because of investigating thefull variety of genome-based analysis such as microbial genetic diversity and population structure played by them. Next generation sequencing technologies can reveal a greater proportion of microbial communities that have not been reported earlier by traditional techniques. The present review highlights the shift from traditional techniquesfor the basic study of community composition to next-generation sequencing (NGS) platforms and their potential applications to the biomonitoring of water quality in relation to human health.

Detection of non-Saccharomyces yeast strains in alcoholic fermentations by direct PCR and/or plating methods

Since the population of indigenous non-Saccharomyces yeasts is displaced from an alcoholic fermentation within a few hours, the use of a selective enrichment broth would be a valid approach for their detection. To accomplish this, yeast carbon base (YCB)–lysine broth without or with different concentrations of cycloheximide was tested. Lysine is an amino acid that cannot serve as the only nitrogen source for Saccharomyces spp., which is in contrast to non-Saccharomyces yeast, while cycloheximide is an antibiotic that is more or less effective against Saccharomyces spp. strains. Metagenomics, the study of genetic material from mixed microbial community as a grape must fermentation has the potential for operating in a culture-independent environment. Direct identification by PCR (dPCR) without previous isolation and a classical approach to analyse the yeast population in a wine inoculated with a mixed population of Saccharomyces cerevisiae and non-Saccharomyces yeasts were compared. Results showed that pre-incubation on YCB–lysine and dPCR combined with classic approaches based on the use of solid media is a suitable strategy for detecting yeast species involved in vinifications.

Intestinal microbiota: its role in digestive diseases.

It is now well known that intestinal microbiota exerts not only several physiological functions, but has also been implied in the mechanisms of many conditions, both intestinal and extraintestinal. These advances, to the best of our knowledge, have been made possible by the development of new ways of studying gut flora. Metagenomics, the study of genetic material taken directly from environmental samples, avoiding individual culture, has become an excellent tool to study the human microbiota. Therefore, it has demonstrated an association between an altered intestinal microbiota and inflammatory bowel disease or irritable bowel syndrome, perhaps the most extensively studied conditions associated with this particular subject. However, microbiota has a potential role in the development of other diseases; their manifestations are not confined to the intestine only. In this article, an extensive updated review is conducted on the role intestinal microbiota has in health and in different diseases. Focus is made on the following conditions: inflammatory bowel disease, irritable bowel syndrome, celiac disease, hepatic encephalopathy, and obesity.

DNA Extraction Systematics for Spectroscopic Studies.

Study of genetic material allows the comprehension the origin of the many biochemical changes that follow diseases, like cancer, promoting the development of early preventive inquiry and more efficient individual treatments. Raman spectroscopy can be an important tool in DNA study, since it allows probe molecular vibrations of genetic material in a fast way. The present work established a systematic way for extract DNA in suitable concentrations and structural integrity allowing studies by Raman spectroscopy or other spectroscopic technique, including bio-analytical sensors for probing genetic alterations.