The study was carried out to investigate the impact and also compare the motivational impact level of sustainable entrepreneurship education on sustainable entrepreneurial intention among public and Private University Undergraduate in Southwest, Nigeria. It adopted survey research design, a sample size of 3,542 out of 26,898 university undergraduates from the selected Public and private universities in Ogun, Osun, and Oyo States were selected. Primary data was sourced using a structured questionnaire. Descriptive statistics was adopted to get the mean and standard deviation of the constructs for the variable under investigation, while Mann-Whitney U-test was used to examine the differences in the impact level and Linear Regression was used to analyse the impact of sustainable entrepreneurship education of sustainable entrepreneurial intention. Findings showed that sustainable entrepreneurship education have significant impact (F1, 3540 = 21187.94; R<sup>2</sup> = 0.8568; P < 0.0000) on Sustainable Entrepreneurial intentions. Also, the public Universities (μ<SUB>R</SUB>=1584.96) students have more of motivational impact level in terms of sustainable entrepreneurship education compared to students in private Universities (μ<SUB>R</SUB>=2035.35). The study recommends based on its findings that the Federal government should also contribute towards putting in place infrastructure for entrepreneurial and skill acquisition in private Universities.
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