<p>Adjustment of adolescents in relation to their gender and religion was investigated. Gender (Male and Female) and religion (Hindu and Muslim) were treated as independent variables whereas adjustment was treated as a dependent variable. The sample comprised of 282 high school students (from class IX and X) of state Government schools of Guwahati city that belonged to lower middle-income group. The multi-stage sampling method was used for selecting the sample. Upadhyay-Saxena Socio-Economic Status (USSES) Scale, Adjustment Inventory for School Students (AISS) and Personal Information Data sheet were administered to all the participants. Mean, Standard Deviation and ANOVA were calculated to analyze the responses. The findings of the study revealed that females are better adjusted in the areas of emotional adjustment, educational adjustment and also in terms of total adjustment in comparison to males, which implies gender has a significant effect in the area of emotional adjustment, educational adjustment and also in the total adjustment of the adolescents. But no significant difference was found in the area of social adjustment between male and female adolescents. Further, it was found that religion has no significant effect on the adjustment among adolescents, which means that there is no significant difference in the adjustment of Hindu and Muslim adolescents of Guwahati city. </p>