This research aims to determine the improvement of creative thinking skills in marine ecology in developing bioentrepreneurship towards sustainable development in prospective biology teacher students. The material in this research is plankton, especially the cultivation of Spirulina sp in the laboratory. The research method used was quasi-experimental with a one-group pretest posttest design. The research sample consisted of 27 students who took a marine ecology course in semester 5 at Central Kalimantan University. The test instruments were multiple choice (PG) reasoned and plankton material description test questions. The research results show that each indicator of creative thinking skills, namely flexibility, originality, and elaboration, is significantly different (p = 0.000). The research results show that plankton material towards sustainable development can improve creative thinking skills in prospective biology teacher students. The contribution of this research can have implications for sustainable development goals point 4, namely quality education, and point 14, namely life underwater.