Cell Structure Research Articles

Genetic Engineering in Bacteria, Fungi, and Oomycetes, Taking Advantage of CRISPR

Genetic engineering has revolutionized our ability to modify microorganisms for various applications in agriculture, medicine, and industry. This review examines recent advances in genetic engineering techniques for bacteria, fungi, and oomycetes, with a focus on CRISPR-Cas systems. In bacteria, CRISPR-Cas9 has enabled precise genome editing, enhancing applications in antibiotic production and metabolic engineering. For fungi, despite challenges associated with their complex cell structures, CRISPR/Cas9 has advanced the production of enzymes and secondary metabolites. In oomycetes, significant plant pathogens, modified Agrobacterium-mediated transformation, and CRISPR/Cas12a have contributed to developing disease-resistant crops. This review provides a comparative analysis of genetic engineering efficiencies across these microorganisms and addresses ethical and regulatory considerations. Future research directions include refining genetic tools to improve efficiency and expand applicability in non-model organisms. This comprehensive overview highlights the transformative potential of genetic engineering in microbiology and its implications for addressing global challenges in agriculture, medicine, and biotechnology.

Fabrication of Hydroxyl Modified Hollow Glass Microsphere Composite Isocyanate‐Based Polyimide Foam and Optimization Strategy Based on Different Bonding Mechanisms

ABSTRACTIn this study, the hydroxyl modified hollow glass microsphere (HM‐HGM) is added to different foaming slurries of isocyanate‐based polyimide foam (IBPIF) at varying ratios, and different bonding effects are formed to optimize the dispersion behavior. Then, the novel HGM composited IBPIF (IBPIF/HGM) is prepared. Hydroxyl groups on HM‐HGM establish hydrogen bonding effect with pyromellitic acid dimethyl ester and dimethyl formamide in the white slurry and react with isocyanate groups in the black slurry. The cell structure of IBPIF is altered to improve its sound absorption performance and mechanical behaviors. Compared with IBPIF/HGM‐0, the average cell size of IBPIF/HGM‐1 and BPIF/HGM‐5 decreases significantly. The sound absorption performance and mechanical behaviors of them are improved to some extent. Compared with samples in which the HM‐HGM is added alone to a single slurry, when the dosage ratio of HM‐HGM in black and white slurries is 1:1, IBPIF/HGM‐3 has more uniform cell structure. The change of IBPIF cell structure by the introduction of HM‐HGM and the unique structure of HM‐HGM can enhance the sound absorption performance and mechanical behaviors of IBPIF. The design idea of different bonding mechanisms significantly provides technical assistance to enhance the acoustic performance of polymeric foam materials.

Correlative light and soft X-ray tomography of in situ mesoscale heterochromatin structure in intact cells

AbstractHeterochromatin organization is critical to many genome-related programs including transcriptional silencing and DNA repair. While super-resolution imaging, electron microscopy, and multiomics methods have provided indirect insights into the heterochromatin organization, a direct measurement of mesoscale heterochromatin ultrastructure is still missing. We use a combination of correlative light microscopy and cryo-soft X-ray tomography (CLXT) to analyze heterochromatin organization in the intact hydrated state of human mammary fibroblast cells. Our analysis reveals that the heterochromatin ultra-structure has a typical mean domain size of approximately 80 nm and a mean separation of approximately 120 nm between domains. Functional perturbations yield further insights into the molecular density and alterations in the mesoscale organization of the heterochromatin regions. Furthermore, our polymer simulations provide a mechanistic basis for the experimentally observed size and separation distributions of the mesoscale chromatin domains. Collectively, our results provide direct, label-free observation of heterochromatin organization in the intact hydrated state of cells.

Abstract 4120919: Cell physiology and multi-omics analysis revealed disordered embryogenesis as early as mesoderm specification in models of Holt-Oram Syndrome

Introduction: Congenital heart diseases (CHDs) are the leading cause of childhood morbidity and mortality. The dysregulation of several cardiac transcription factors (TFs) leads to CHD. The coordination of several cardiac TFs is required and essential for cardiogenesis. However, the mechanisms to acquire its cardiac cell identity remain unclear. Hypothesis: Mutant Tbx5 dysregulates embryogenesis during mesoderm specification, prior to its expression in the tissues in Holt-Oram syndrome. Methods: Using cellular physiology and multiomics analysis in both human ES cells and a zebrafish model of TBX5 germline mutation. we evaluated embryonic structure and function, prior to the onset of gastrulation in the context of both heterozygous and homozygous TBX5 mutation. Results: Zebrafish time course transcriptome profiles over gastrulation revealed that loss of Tbx5 impacted transcriptional profiles at the blastula stage. Single cell RNA sequencing (scRNA-seq) on 16243 zebrafish blastula stage cells showed loss of Tbx5 impacted transcription noise at the blastula stage prior to the expression of zygotic Tbx5 with associated effects on chromatin accessibility using omni-assay for transposase-accessible chromatin (ATAC) and evidence of aberrant Wnt signaling even at the blastula stage. Embryo-wide cell structure and function were abnormal in both Tbx5 mutant zebrafish heterozygotes or homozygotes. To validate Undifferentiated human ES cell H3K4me3 Cleavage Under Targets&Release Using Nuclease (CUT&RUN) also showed abnormal Wnt signaling in TBX5 homozygous mutation and TBX5 CUT&RUN showed aberrant mesoderm pathway. Calcium imaging analysis demonstrated the lowest excitation frequency in TBX5 homozygous mutation prior to mesoderm specification. TBX5 homozygous human ES derived mesoderm cells exhibited low expression levels of mesoderm marker genes compared to TBX5 wild type mesoderm. Conclusion: Integrating single cell physiology and multi-omics technologies, we idneifified fundamental dysregulation of embryogenesis in mutant cardiac-restricted gene disorder prior to mesoderm specification or the zygotic expression of the mutant gene. These findings suggest that Tbx5 started to determine cell fate prior to mesoderm specification by altering chromatin accessibilities and histone modification. Tbx5 affected mesoderm differentiation by influencing Wnt signaling and cell physiology.

Optimized Graph Sample and Aggregate‐Attention Network‐Based High Gain Meta Surface Antenna Design for IoT Application

ABSTRACTThis paper presents a High Gain Metasurface (HGM) antenna design optimized for IoT applications. The antenna operates at a 5.8 GHz frequency and utilizes a quarter‐wavelength unit cell structure. The design employs a Graph Sample and Aggregate‐Attention Network (GSAAN) to enhance the transmission characteristics of the metasurface. To optimize the performance of GSAAN, the Giza Pyramids Construction Algorithm (GPCN) is applied to adjust the weight parameters, leading to significant improvements in radiation efficiency, bandwidth, gain, directivity, and return loss. The proposed HGM antenna is simulated in MATLAB 2023b, where it demonstrates substantial performance gains over existing methods. Specifically, the proposed design achieves 28.34% higher gain compared to the MSA‐RHCOA method, 24.47% improvement over HGM‐AD‐MATCS, 26.34% better gain than BR‐HGA‐PGM, and a 32.12% gain increase relative to MSA‐Hyb‐ADSA‐HMSOA. These enhancements make the proposed antenna design a competitive solution for high‐gain applications in wireless communication, satellite communication, and radar systems. The integration of GSAAN with GPCN optimization in the HGM antenna design enables the creation of highly directive radiation patterns, making it well‐suited for IoT applications that require high performance and reliability. The results of this study demonstrate the effectiveness of the proposed approach in achieving superior antenna performance, positioning it as a strong alternative to existing metasurface antenna designs.

Inactivation of Pichia membranaefaciens in Soybean Paste by Dual-Frequency and Moderate Thermosonication

Dual-frequency and moderate thermosonication (TS, 300 + 300 W, 20 + 40 kHz, 25~60 °C) was employed to inactivate Pichia membranifaciens in soybean paste. The aim was to evaluate the effect of TS on the inactivation of P. membranaefaciens and on the quality of soybean paste. The Weibull model fitted the survival data of P. membranaefaciens in thermosonicated soybean paste well and a decrease of 5 log of P. membranaefaciens in soybean paste was obtained at TS50°C, TS55°C, TS60°C, and T65°C for 15.41, 7.49, 2.27, and 18.61 min. Scanning electron microscope observation revealed TS50°C damaged the cell structure, leading to the leakage of intracellular contents. The physicochemical properties of soybean paste treated by TS were more retained than in paste treated by heat. The GC-MS analysis indicated that the flavor components had increased after TS treatment, especially at TS50°C. In conclusion, TS can inactive P. membranaefaciens in soybean paste without causing significant changes in its physicochemical and flavor qualities.

Biothermodynamics of Hemoglobin and Red Blood Cells: Analysis of Structure and Evolution of Hemoglobin and Red Blood Cells, Based on Molecular and Empirical Formulas, Biosynthesis Reactions, and Thermodynamic Properties of Formation and Biosynthesis

Biothermodynamics of Hemoglobin and Red Blood Cells: Analysis of Structure and Evolution of Hemoglobin and Red Blood Cells, Based on Molecular and Empirical Formulas, Biosynthesis Reactions, and Thermodynamic Properties of Formation and Biosynthesis

Staining and resin embedding of whole Daphnia magna samples for micro-CT imaging enabling 3D visualization of cells, tissues, and organs.

Micro-CT imaging is a powerful tool for generating high-resolution, isotropic, three-dimensional datasets of whole, centimeter-scale model organisms. At histological resolutions, micro-CT can be used for whole-animal qualitative and quantitative characterization of tissue and organismal structure in health and disease. The small size, global freshwater distribution, wide range of cell size and structures of micron scale, and common use of Daphnia magna in toxicological and environmental studies make it an ideal model for demonstrating the potential power of micro-CT-enabled whole-organism phenotyping. This protocol details the steps involved in D. magna samples preparation for micro-CT, including euthanasia, fixation, staining, and resin embedding. Micro-CT reconstructions of samples imaged using synchrotron micro-CT reveal histological (microanatomic) features of organ systems, tissues, and cells in the context of the entire organism at sub-micron resolution and in 3D. The enabled "3D histology" and 3D renderings can be used for morphometric analyses across cells, tissues, and organ systems.

Electron microscopy method in assessing the quality of cell cryopreservation

During its existence, electron microscopy has become one of the reference methods for assessing the structural and functional state of cells, tissues and organs, and an extensive evidence base has been formed that allows its use in the creation and formation of a biobank. The selection of sources for the literature review was carried out using keywords based on publications over the past 20 years. The publications presented in the review were selected by searching the eLIBRARY.RU, PubMed and Scopus databases. Based on foreign and domestic experience in the work of biobanks, we can distinguish four areas that determine the effectiveness of the use of electron microscopy studies as a component of its work. Firstly, it is control of microbiological contamination of a biological sample. The effectiveness of electron microscopy in detecting contamination of a biological sample with bacteria, fungi and viruses is comparable to the effectiveness of classical microbiological techniques. Secondly, it is a diagnostic tool that allows you to identify or confirm the presence in a sample of a pathogenetic process that is of interest for biobanking: tumor growth, atherosclerotic vessel damage, etc. Thirdly, it is quality control for cryopreservation of samples. A wide range of morphological characteristics of the ultramicroscopic structure of cells and microanatomical formations makes it possible to characterize the quality of cryopreservation and quantify the degree of damage, which contributes to the unification and standardization of biobanking. Transmission electron microscopy is the most informative in this matter. Fourthly, this is the basis for digitalization of the results obtained and the formation of an interdisciplinary biobank repository, which allows the use of “big date” technologies for fundamental research. The compliance of the biobank profile with the economic, scientific and industrial characteristics of the infrastructure of the industry or region is of great importance. Electron microscopy data are successfully combined with the results of molecular studies, which allows the formation of interdisciplinary metadata databases suitable for interregional and interdisciplinary scientific integrations. The latter makes it possible to use electron microscopy data to solve a wide range of applied and interdisciplinary problems. The above allows us to consider scanning and transmission microscopy methods as one of the key methods in the development of biobanking in the region.

Hybrid poly(lactide-co-glycolide) membranes incorporated with doxycycline-loaded copper-based metal-organic nanosheets as antibacterial platforms.

The rise of antimicrobial resistance necessitates innovative strategies to combat persistent infections. Metal-Organic Frameworks (MOFs) have attracted significant attention as antibiotic carriers due to their high drug loading capacity and structural adaptability. In particular, 2D MOF nanosheets are emerging as a notable alternative to their traditional 3D relatives due to their remarkable advantages in enhanced surface area, flexibility and exposed active region properties. Herein, we synthesized 2D Copper 1,4-benzendicarboxylate (CuBDC) nanosheets and utilized them as a carrier and controlled release system for Doxycycline (Doxy@CuBDC), for the first time. The Doxy@CuBDC nanosheets were subsequently incorporated into poly (DL-lactide-co-glycolide) (PLGA) electrospun membranes (Doxy@CuBDC/PLGA). The resultant bioactive fibrous membranes exhibited double-barrier controlled release properties, extending the Doxy release up to ∼9 days at pH 7.4 and 5.5. Significant inhibitory effects against Staphylococcus aureus and Escherichia coli were observed. The morphological analyses revealed the deformed bacterial cell structures on Doxy@CuBDC/PLGA membranes that indicates potent bactericidal activity. Furthermore, cytotoxicity assays demonstrated the non-toxic nature of the fabricated membranes, underscoring their potential use for biomedical applications. Overall, the hybrid antibacterial PLGA membranes present a promising strategy for combating microbial infections while maintaining biocompatibility and offer a versatile approach for biomedical material design and surface coatings (e.g., wound dressings, implants).&#xD.

Analysis on future development of solar cells focusing on materials and devices

In recent times, solar cells have garnered significant attention due to their numerous advantages. These include continuously improving energy conversion efficiency, a simple solution preparation method, flexibility, lightweight design, wearability, suitability for ultra-lightweight space applications, and low-cost material components. Perovskite solar cells have achieved impressive efficiencies of over 25% by using high-quality perovskite films synthesized at low temperatures and by making advancements in compatible interface and electrode materials. Moreover, the stability of cells has become a major focus of research. This paper analyzes the development history, material system, device structure, challenges, and future development direction of perovskite solar cells. The unique photoelectric properties and tunable band gap of materials have made them a hot topic in the field of solar cells. In this paper, the basic characteristics and synthesis methods of perovskite materials are introduced before discussing the device structure, interface engineering, and stability of perovskite solar cells in detail. Finally, it prospects on the future development trend of perovskite solar cells.

A Rationally Designed Azobenzene Photoswitch for DNA G‐Quadruplex Regulation in Live Cells

AbstractG‐quadruplex (G4) DNA structures are increasingly acknowledged as promising targets in cancer research, and the development of G4‐specific stabilizing compounds may lay a fundamental foundation in precision medicine for cancer treatment. Here, we propose a light‐responsive G4‐binder for precise modulation of drug activation, providing dynamic and spatiotemporal control over G4‐associated biological processes contributing to cancer cell death. We developed a specialized fluorinated azobenzene (AB) switch equipped with a quinoline unit and a positively charged carboxamide side chain, Q‐Azo4F‐C, designed for targeted binding to G4 structures within cells. Biophysical studies, combined with molecular dynamics simulations, provide insights into the unique coordination modes of the photoswitchable ligand in its trans and cis configurations when interacting with G4s. The observed variations in complexation processes between the two isomeric states in different cancer cell lines manifest in more than 25‐fold reversible cytotoxic activity. Immunostaining conducted with the structure‐specific G4 antibody (BG4), establishes a direct correlation between cytotoxicity and the varying extent of G4 induction regulated by the two isoforms. Finally, we demonstrate the photo‐driven reversible regulation of G4 structures in lung cancer cells by Q‐Azo4F‐C. Our findings highlight the potential of light‐responsive G4‐binders in advancing precision cancer therapy through dynamic control of G4‐mediated pathways.

Microstructure instability of additively manufactured alloy 718 fabricated by laser powder bed fusion during thermal exposure at 600–1000 ℃

Microstructure and precipitation behavior of alloy 718 produced by laser powder bed fusion (L-PBF) and its recrystallized counterpart were evaluated after thermal exposure at a temperature range of 600–1000 ℃ for up to 1000 h. The as-built 718 featured a microstructure of columnar grains, and dislocation cell structures (DCSs) with chemical segregations (rich in Nb, Ti, Mo and depleted in Cr, Fe) and precipitates (Laves phase and carbonitride). The DCSs remained thermally stable for up to 1000 h at 600 ℃ or 100 h at 700 ℃, but only 10 h at 800 ℃. With the temperature increasing to 900 ℃ and 1000 ℃ for 1 h duration, the DCSs tended to partially disassemble, and the chemical segregations were removed. The chemical segregations at cell boundaries of the as-built 718 promoted the formation of γ′, γ″ and δ phases during aging, leading to a spatially heterogeneous distribution of γ′ and γ″ phases between the cell boundaries and interiors during short exposure time or at lower temperatures. Meanwhile, the coarsening of γ′ and γ″ phases and the phase transformation of γ″ to δ at 700–800 ℃ were facilitated in the as-built 718 compared to the recrystallized ones. A unique precipitation distribution was observed at 700 ℃ that γ′ and γ″ phases precipitated both at the cell boundaries and within cell interiors, while only γ′ formed in the vicinity of cell boundaries. Time-temperature-transformation curves for additively manufactured and recrystallized 718 were constructed based on the microstructure analyses. This study indicates that conventional heat treatment procedures may be suboptimized for additively manufactured 718 in high temperature applications.

A Comprehensive Approach to Optimization of Silicon-Based Solar Cells

In this work, we report a detailed scheme of computational optimization of solar cell structures and parameters using PC1D and AFORS-HET codes. Each parameter’s influence on the properties of the components of heterojunction silicon-based solar cells (HIT) has been thoroughly examined. The proposed approach follows a stringent sequence of steps to optimize various parameters of the studied HITs. Furthermore, we have revealed the effects of the metal-semiconductor contact, and a model of a photocell with an ohmic contact and a Schottky contact has been simulated. The optimal model of HIT for available materials has been proposed and fabricated based on the results of these simulations. A comparison of predicted and measured performance unequivocally demonstrates the efficiency of the proposed scheme in developing silicon-based HITs, providing reassurance about its practical application.

Versatile silicate-modified hydrochar based on Scutellaria baicalensis-residue for efficiently removing heavy metal-antibiotic co-contamination and relevant bio-contaminants from wastewater

In this study, we utilized typical Chinese medicine herbal residues (CMHRs)－Scutellaria baicalensis (Scutellaria baicalensis Georgi) residue (SR), as the raw material and employed Na2SiO3 and Fe2(SO4)3 as modifying agents to fabricate a novel and multifunctional hydrochar (FeSi-SRHC), which was designed for comprehensive removal of typical contaminants such as Cu2+, Zn2+, tetracycline (TC), and ciprofloxacin (CIP), along with relevant bio-contaminants (resistant bacteria (RBs) and resistance genes (RGs)) present in wastewater. Based on the analysis of adsorption kinetics and Freundlich isotherm, it was found that FeSi-SRHC exhibited physical monolayer adsorption for Cu2+/Zn2+ while mainly chemical multilayer adsorption for TC/CIP in single-contamination system. Furthermore, Langmuir isotherm demonstrated excellent adsorption capacity of FeSi-SRHC towards Cu2+/Zn2+/TC/CIP with maximum capacities of 255.75, 265.26, 425.53, and 404.86 mg/g, respectively. In the co-contamination system, the presence of Cu2+, Zn2+, TC, and CIP exhibited varying degrees of inhibitory or promotive effects on the mutual adsorption by FeSi-SRHC. This divergence stemmed from differences in complexation intensities and concentration ratios among diverse co-existing contaminants. Based on XPS and Density Functional Theory (DFT) analyses, the adsorption process for Cu2+, Zn2+, TC, and CIP by FeSi-SRHC primarily involves pore fill, complexation reactions, ion exchange, hydrogen bonding, π-π stacking interactions, and electrostatic interactions. Bio-contaminant removal experiments revealed that the release of baicalin and wogonoside from FeSi-SRHC disrupts the structure of RBs cells and compromises the integrity of resistance plasmids. The practical application experiment showed that FeSi-SRHC displayed favorable performance for removing heavy metals, antibiotics, and bio-contaminants in actual wastewater. This study presented a “Treating waste with waste” strategy, which provided a method with low carbon, eco-friendly, and inexpensive for CMHRs resources and turning waste into treasure while proposing a idea to address the challenges associated with treating heavy metal, antibiotic, and bio-contaminant contamination in wastewater.

A Horizontal Double‐Sided Copper Metallization Technology Designed for Solar Cell Mass‐Production

ABSTRACTIn the international photovoltaic market, the “carbon footprint” label has received great attention, and low‐carbon footprint products are widely popular. Compared with metallic silver, copper has the characteristics of low carbon emissions and low cost, which can effectively reduce the carbon footprint. Based on this, this article reports a horizontal double‐sided copper metallization technology. This technology can not only metalize the front and back sides of various types of silicon solar cells at the same time but also has fast speed, good uniformity, and simple process, making it suitable for the industrial mass production of solar cells. This article describes the structure and manufacturing process of TOPCon solar cells patterned with an ultrashort pulse laser and metalized using this novel horizontal double‐sided copper metallization technology. In this batch, average efficiency reached above 26%.

Exploring new lead-free halide perovskites RbSnM3 (M = I, Br, Cl) and achieving power conversion efficiency > 32 %

Lead-free ABX3 inorganic perovskites, where A = Cs, Rb; BSn, Ge; and X = I, Br, Cl, have recently gained significant attention due to their remarkable optical, structural, and electronic properties, as well as their potential for solar cell applications. In this study, we thoroughly examined the optical, structural, and electronic properties of RbSnM3 (M = I, Br, Cl) perovskites through first-principles calculations and explored their application in a HTL-free solar cell structure using SCAPS-1D. Our analysis revealed that RbSnI3, RbSnBr3, and RbSnCl3 have direct band gaps of 0.828, 0.988, and 1.242 eV, respectively, using the HSE functional. The electron charge distribution indicates a strong ionic bond between Rb and the halides, as well as a significant covalent bond between Sn and the halides. Additionally, we calculated optical properties such as electron loss function, absorption coefficients, and the real and imaginary parts of the dielectric functions. We also explored the photovoltaic performance of RbSnM3 absorbers paired with a SnS2 ETL layer, investigating different thicknesses, defect densities, doping concentrations, and interface defect densities. The highest power conversion efficiencies (PCE) achieved were 26.38 %, 29.79 %, and 32.53 % for RbSnI3, RbSnBr3, and RbSnCl3 absorber layers, respectively, when paired with a SnS2 ETL. Overall, RbSnCl3 stands out as a highly promising absorber material for future photovoltaic devices, especially when combined with the SnS2 ETL layer.

The Effect of the Acid-Base Imbalance on the Shape and Structure of Red Blood Cells.

Red blood cells respond to fluctuations in blood plasma pH by changing the rate of biochemical and physical processes that affect the specific functions of individual cells. This study aimed to analyze the effect of pH changes on red blood cell morphology and structure. The findings revealed that an increase or decrease in pH above or below the physiological level of pH 7.4 results in the transformation of discocytes into echinocytes and causes significant alterations in the membrane, including its roughness, cytoskeleton structure, and the cell's elastic modulus. Furthermore, the study shown a strong connection between critical acidosis and alkalosis with increased intracellular reactive oxygen species production.

Crucial Roles of Brassinosteroids in Cell Wall Composition and Structure Across Species: New Insights and Biotechnological Applications.

Brassinosteroids (BR) are steroidal phytohormones essential for plant growth, development, and stress resistance. They fulfil this role partially by modulating cell wall structure and composition through the control of gene expression involved in primary and secondary cell wall biosynthesis and metabolism. This affects the deposition of cellulose, lignin, and other components, and modifies the inner architecture of the wall, allowing it to adapt to the developmental status and environmental conditions. This review focuses on the effects that BR exerts on the main components of the cell wall, cellulose, hemicellulose, pectin and lignin, in multiple and relevant plant species. We summarize the outcomes that result from modifying cell wall components by altering BR gene expression, applying exogenous BR and utilizing natural variability in BR content and describing new roles of BR in cell wall structure. Additionally, we discuss the potential use of BR to address pressing needs, such as increasing crop yield and quality, enhancing stress resistance and improving wood production through cell wall modulation.

Enhancement in the Electrical Properties and Electromagnetic Interference Shielding Performance of Acrylonitrile–Butadiene–Styrene/Carbon Nanotubes Foams via the Introduction of Bimodal Cell Structures

The cell structures of conductive polymer‐based composite foams significantly influence their electrical properties and electromagnetic interference (EMI) shielding effectiveness (SE), necessitating a thorough understanding of how these properties improve with evolving cell structures. In this study, supercritical CO2 foaming technique was manipulated to fabricate acrylonitrile–butadiene–styrene (ABS)/carbon nanotubes (CNTs) foams. The constant‐temperature mode was used to prepare unimodal foams (UF), while the bimodal foams (BF) were produced by varying‐temperature mode. The foaming properties, electrical conductivity, complex permittivity, and EMI SE of ABS/CNTs foams with various cell structures are methodically investigated at identical volume expansion ratio and CNTs content. The electrical conductivity of bimodal ABS/CNTs foam with CNTs content of 20% (BF‐C20) is 0.2191 S cm−1, higher than that of unimodal ABS/CNTs foam with CNTs content of 20% (UF‐C20) (0.1765 S cm−1) owing to the introduction of bimodal cell structures. Complex permittivity results manifest that at 8.2 GHz, the ε′ and ε″ of BF‐C20 are 67.7 and 74.5, respectively, which are higher than 57.9 and 52.9 of UF‐C20. Among all ABS/CNTs foams, the total EMI SE of BF‐C20 attains the highest EMI shielding value, which reaches 30.2 dB. Furthermore, the absolute shielding effectiveness of BF‐C20 is 188.5 dB (g cm−2)−1, which is 17.3% higher than that of UF‐C20.