Structure Of Outer Layer Research Articles

Retinal G-protein-coupled receptor deletion exacerbates AMD-like changes via the PINK1-parkin pathway under oxidative stress.

The intake of high dietary fat has been correlated with the progression of age-related macular degeneration (AMD), affecting the function of the retinal pigment epithelium through oxidative stress. A high-fat diet (HFD) can lead to lipid metabolism disorders, excessive production of circulating free fatty acids, and systemic inflammation by aggravating the degree of oxidative stress. Deletion of the retinal G-protein-coupled receptor (RGR-d) has been identified in drusen. In this study, we investigated how the RGR-d exacerbates AMD-like changes under oxidative stress, both invivo and invitro. Fundus atrophy became evident, at 12 months old, particularly in the RGR-d + HFD group, and fluorescence angiography revealed narrower retinal vessels and a reduced perfusion area in the peripheral retina. Although rod electroretinography revealed decreasing trends in the a- and b-wave amplitudes in the RGR-d + HFD group at 12 months, the changes were not statistically significant. Mice in the RGR-d + HFD group showed a significantly thinner and more fragile retinal morphology than those in the WT + HFD group, with disordered and discontinuous pigment distribution in the RGR-d + HFD mice. Transmission electron microscopy revealed a thickened Bruch's membrane along the choriocapillaris endothelial cell wall in the RGR-d + HFD mice, and the outer nuclear layer structure appeared disorganized, with reduced nuclear density. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated significantly lower levels of 25(OH)-vitamin D3 metabolites in the RGR-d + HFD group. Under oxidative stress, RGR-d localized to the mitochondria and reduced the levels of the PINK1-parkin pathway. RGR-d mice fed an HFD were used as a new animal model of dry AMD. Under high-fat-induced oxidative stress, RGR-d accumulated in the mitochondria, disrupting normal mitophagy and causing cellular damage, thus exacerbating AMD-like changes both invivo and invitro.

Heart-on-a-Chip Model of Epicardial-Myocardial Interaction in Ischemia Reperfusion Injury.

Epicardial cells (EPIs) form the outer layer of the heart and play an important role in development and disease. Current heart-on-a-chip platforms still do not fully mimic the native cardiac environment due to the absence of relevant cell types, such as EPIs. Here, using the Biowire II platform, engineered cardiac tissues with an epicardial outer layer and inner myocardial structure are constructed, and an image analysis approach is developed to track the EPI cell migration in a beating myocardial environment. Functional properties of EPI cardiac tissues improve over two weeks in culture. In conditions mimicking ischemia reperfusion injury (IRI), the EPI cardiac tissues experience less cell death and a lower impact on functional properties. EPI cell coverage is significantly reduced and more diffuse under normoxic conditions compared to the post-IRI conditions. Upon IRI, migration of EPI cells into the cardiac tissue interior is observed, with contributions to alpha smooth muscle actin positive cell population. Altogether, a novel heart-on-a-chip model is designed toincorporate EPIs through a formation process that mimics cardiac development, and this work demonstrates that EPI cardiac tissues respond to injury differently than epicardium-free controls, highlighting the importance of including EPIs in heart-on-a-chip constructs that aim to accurately mimic the cardiac environment.

Origin of surface oxidation induced the nucleation and propagation of microcracks in TNM alloy

This study investigated the impact of surface degradation caused by oxygen (O) and nitrogen (N) elements in the environmental atmosphere on the service failure of nearly lamellar TNM alloy. The results show that oxidation-induced phase transformations at the surface and subsurface regions strongly affect the alloy's tensile properties at high temperatures. The precipitation of Al2O3, which disrupts the integrity of the Ti2AlN layer, has a weaker deformation resistance capacity compared to the Z-phase. Under stress conditions, numerous dislocations concentrate at the Al2O3 phase, causing Al2O3 particles to easily detach and initiating crack nucleation at the Z-phase/Al2O3 interface. This is the origin of oxidation failure. Cracks nucleate at the Al2O3 detachment region, propagate, and connect with surface microcracks induced by the rough and loose outer oxide layer structure. This leads to the rapid propagation of cracks into the subsurface. The consumption of β-stabilizing elements at the surface, along with the internal diffusion of O and N elements into the subsurface region, results in the formation of the hard brittle Z-phase at the subsurface lamellar structure and a transition from β0 to α2 at the lamellar colony boundaries. The precipitation of α2 and Z-phase also accelerates crack propagation. In summary, the failure mode shifts from toughness fracture to brittle fracture.

Unlocking therapeutic potential: dual gene therapy for ameliorating the disease phenotypes in a mouse model of RPE65 Leber congenital amaurosis.

Leber congenital amaurosis (LCA) is the most common genetic cause of congenital visual impairment in infants and children. Patients with LCA who harbor RPE65 mutations exhibit a deficiency in photoreceptor rhodopsin, leading to severe night blindness and visual impairment following birth. Since either gene replacement therapy or anti-apoptosis therapy alone cannot maintain both functional and morphological normality for a long time in the animal model, we propose a robust treatment strategy, that is, gene replacement therapy combined with anti-apoptotic therapy to protect photoreceptors from further degeneration while compensating for lost RPE65 function. Here, rd12 mice were injected subretinally at postnatal day 14 with four vector administrations, respectively. At 6 months after treatment, it was discovered that injection of three vectors, AAV8 (Y733F)-CBA-hRPE65, AAV8(Y733F)-CBA-hRPE65-BCL-2-L10 and mixture of half-dose AAV8(Y733F)-CBA-hRPE65 and half-dose AAV8 (Y733F)-CBA-BCL-2-L10, could partially restore the visual function of rd12 mice. Meanwhile, these treated eyes also exhibited a thicker outer nuclear layer (ONL) structure. However, despite the fact that the eyes of rd12 mice injected with the AAV8 (Y733F)-CBA-BCL-2-L10 vector displayed a slightly thicker ONL structure compared to untreated eyes, the visual function of the treated eyes did not recover. Continuing the observation period to 12 months after treatment, we found that compared to rd12 mice at 6-month post-treatment, rd12 mice injected with AAV8 (Y733F)-CBA-hRPE65 or mixture of half-dose AAV8(Y733F)-CBA-hRPE65 and half-dose AAV8 (Y733F)-CBA-BCL-2-L10 exhibited varying degrees of decline in both visual function and ONL thickness. However, in the case of rd12 mice injected with the AAV8(Y733F)-CBA-hRPE65-BCL-2-L10 vector, the ONL thickness remains consistent at both 6 and 12 months after treatment. These mice continued to maintain a relatively strong visual function and showed restoration in the levels of RPE65 and Rhodopsin protein expression. Our findings illustrate that early postnatal treatment with AAV vectors containing both the hRPE65 gene and the Bcl-2L10 anti-apoptotic gene provide enhanced and sustained retinal protection.

Mechanical Factors Regulate Annulus Fibrosus (AF) Injury Repair and Remodeling: A Review.

Low back pain is a common chronic disease that can severely affect the patient's work and daily life. The breakdown of spinal mechanical homeostasis caused by intervertebral disc (IVD) degeneration is a leading cause of low back pain. Annulus fibrosus (AF), as the outer layer structure of the IVD, is often the first affected part. AF injury caused by consistent stress overload will further accelerate IVD degeneration. Therefore, regulating AF injury repair and remodeling should be the primary goal of the IVD repair strategy. Mechanical stimulation has been shown to promote AF regeneration and repair, but most studies only focus on the effect of single stress on AF, and lack realistic models and methods that can mimic the actual mechanical environment of AF. In this article, we review the effects of different types of stress stimulation on AF injury repair and remodeling, suggest possible beneficial load combinations, and explore the underlying molecular mechanisms. It will provide the theoretical basis for designing better tissue engineering therapy using mechanical factors to regulate AF injury repair and remodeling in the future.

A ROS-responsive microsphere capsule encapsulated with NADPH oxidase 4 inhibitor ameliorates macrophage inflammation and ferroptosis

Inflammatory macrophages within the synovium play a pivotal role in the progression of arthritis inflammation. Effective drug therapy targeting inflammatory macrophages has long been a goal for clinicians and researchers. The standard approach for treating osteoarthritis (OA) involves systemic treatment and local injection. However, the high incidence of side effects associated with long-term drug administration increases the risk of complications in patients. Additionally, the rapid clearance of the joint cavity poses a biological barrier to the therapeutic effect. NADPH oxidase 4 (NOX4) is an enzyme protein regulating the cellular redox state by generating reactive oxygen species (ROS) within the cell. In this study, we designed and fabricated a hydrogel microsphere consisting of methyl methacrylate (MMA) and polyvinyl acetate (PVA) as the outer layer structure. We then loaded GLX351322 (GLX), a novel selective NOX4 inhibitor, into hydrogel microspheres through self-assembly with the compound polyethylene glycol ketone mercaptan (mPEG-TK) containing a disulfide bond, forming nanoparticles (mPEG-TK-GLX), thus creating a two-layer drug-loaded microspheres capsule with ROS-responsive and slow-releasing capabilities. Our results demonstrate that mPEG-TK-GLX@PVA-MMA effectively suppressed TBHP-induced inflammation, ROS production, and ferroptosis, indicating a promising curative strategy for OA and other inflammatory diseases in the future.

Stomiosphaerina bakae sp. nov., a new calcareous dinocyst of the Upper Cretaceous of the Central European Basin.

In this work the new species of Stomiosphaerina bakae sp. nov. is described. This is the third species of the genus Stomiosphaerina. Several dozen specimens of the newly described species were found within the Upper Cretaceous (upper Turonian) white chalk at Dubivtsi section near Halych in western Ukraine (south-eastern margin of Central European Basin). Stomiosphaerina bakae sp. nov. has an asymmetrical oval (pear-like shape) test, as it is wider in one side, and sharp in the opposite side, ranging from 55 to 67 μm in length and from 42 to 56.5 μm in width. It has a two-layered calcareous test. The outer layer, with thickness ranging from 4.5 to 6.5 μm, is built of long and wide plate-shaped calcite crystals and is white in plain-polarized light with a dark cross in crossed-polarized light. The inner layer, with thickness ranging from 1.5 to 4.5 μm, is yellowish, goldish to brownish color in plain-polarized light, with proximal and distal side smooth and even, and is built of short fibrous calcite crystals, without preferential orientation. One narrow (6 to 8 μm width) aperture is observed. Stomiosphaerina bakae sp. nov. differs from other species of Stomiosphaerina, i.e. Stomiosphaerina biedai Nowak 1974 and Stomiosphaerina proxima Řehánek 1987, by its cyst-shape, cyst-size, layers thickness, structure of outer layer and width of aperture. Stomiosphaerina bakae sp. nov. most likely corresponds to the non-defined species of Stomiosphaerina sp. described by Nowak 1974.

MOF衍生的单原子催化剂中γN原子在ORR反应中的远程作用及其调制机制

The development of fuel cells is conducive to achieving carbon peaking and carbon neutrality goals, but the oxygen reduction reaction (ORR) of its cathodes is still kinetically slow due to multi-step proton-coupled electron transfer. Currently, metal-organic frameworks-derived single-atom catalysts (SACs) are emerging as efficient ORR catalysts. However, the outer layer structure of the active site is difficult to be determined, resulting in the uncertainty of the reaction mechanism. Here, the dual effect of the outer γN atom on its neighboring βC atom and the distant metal center was investigated. The results show that the doping of γN with different locations and amounts changes the electronic configuration of the replaced C sites in Fe-N4/C-γNx structures, which in turn affects the charge distribution of the βC atom closer to the metal center. And these changes indirectly affect the electron distribution and spin of the metal center through long-range effects, significantly enhancing the ORR performance of the 4-e− paths. More importantly, the electronegativity-enhanced βC atoms are thus more likely to adsorb the OH moiety in alkaline electrolyte typically used in experiments. Thus, a γN-modulated βC-assisted pathway was predicted and a higher thermodynamic limiting potential of ORR than that of the common 4-e− pathway was achieved. Further wavefunction analysis, based on the potential limiting steps, provided an understanding on the electronic level and revealed the significance of precise design and characteristics of γN site in analogous SACs.

Repopulated retinal microglia promote Müller glia reprogramming and preserve visual function in retinal degenerative mice.

Rationale: Müller glia (MG) play a key role in maintaining homeostasis of the retinal microenvironment. In zebrafish, MG reprogram into retinal progenitors and repair the injured retina, while this MG regenerative capability is suppressed in mammals. It has been revealed that microglia in zebrafish contribute to MG reprogramming, whereas those in mammals are over-activated during retinal injury or degeneration, causing chronic inflammation, acceleration of photoreceptor apoptosis, and gliosis of MG. Therefore, how to modulate the phenotype of microglia to enhance MG reprogramming rather than gliosis is critical. Methods: PLX3397, a colony-stimulating factor 1 receptor inhibitor, was applied to deplete microglia in the retinas of retinal degeneration 10 (rd10) mice, and withdrawal of PLX3397 was used to induce the repopulated microglia (Rep-MiG). The protective roles of the Rep-MiG on the degenerative retina were assessed using a light/dark transition test, and scotopic electroretinogram recordings. Immunofluorescence, western blot, transcriptomic sequencing, and bioinformatics analysis were performed to investigate the effects and mechanisms of microglia on MG reprogramming. Results: Following PLX3397 withdrawal, Rep-MiG replenished the entire retina with a ramified morphology and significantly improved the retinal outer nuclear layer structure, the electroretinography response, and the visual behavior of rd10 mice. Coincidentally, MG were activated, de-differentiated, and showed properties of retina progenitors in a spatial correlation with Rep-MiG. Morphological and transcriptomic analyses revealed Rep-MiG significantly enhanced protease inhibitor activity and suppressed extracellular matrix (ECM) levels during retinal degeneration. Conclusions: It suggested that Rep-MiG with the homeostasis characteristic stimulated the progenitor cell-like properties of MG, probably through regulating ECM remodeling, which protected photoreceptors and improved visual function of rd10 mice. It might be a potential protocol to reprogram MG and delay mammal retinal degeneration.

Study on internal structure and digestibility of jackfruit seed starch revealed by chemical surface gelatinization

To understand the contribution of the molecular structure to the digestibility of jackfruit seed starches (JSS) from a new perspective, the starch samples were subjected to chemical surface gelatinization by 4.0 M CaCl 2 solution. After the removal of surface gelatinized part, the remaining JSS granules were obtained with approximately 0%, 15%, 30%, 40%, 50%, 60%, and 70% gelatinization degrees (DG), respectively. The DG values of these starches were calculated by integral optical density (IOD) method that was more accurate and efficient compared to traditional weighing method. The structure features (amylose content, particle morphology, relative crystallinity, short-range ordered structure, thermal properties) and in vitro digestion of JSS were studied and correlated as Pearson's matrix. The morphology of remaining JSS granules showed a rough surface with a lamella structure. The distribution of amylose content in the middle layer was slightly higher than that in the periphery and tended to be more uniform. The resistant starch content of JSS granules was in a fluctuant decrease trend from the periphery to the core, which was consistent with the distribution of relative crystallinity. The remaining JSS granules with higher degrees of gelatinization exhibited lower gelatinization temperature and enthalpy. The results imply that the JSS is hydrolyzed by hydrolase from the dense periphery to the relatively loose interior. And, the lower susceptibility of starch to hydrolase is mainly ascribed to the stable double helix structure of outer layer and alternating crystalline structure. • Surface gelatinized remaining jackfruit seed starch granules were obtained. • Integral optical density method was practical to monitor the gelatinization degree. • The molecular structure and digestibility were correlated as Pearson's matrix. • The relative crystallinity is related to resistant starch of jackfruit seed starch. • Jackfruit seed starch was hydrolyzed from the dense periphery to loose interior.

A study of degradation mechanisms in PVDF-based photovoltaic backsheets

Commercial backsheets based on polyvinylidene fluoride (PVDF) can experience premature field failures in the form of outer layer cracking. This work seeks to provide a better understanding of the changes in material properties that lead to crack formation and find appropriate accelerated tests to replicate them. The PVDF-based backsheet outer layer can have a different structure and composition, and is often blended with a poly(methyl methacrylate) (PMMA) polymer. We observed depletion of PMMA upon aging with sequential (MAST) and combined (C-AST) accelerated stress testing. In field-aged samples from Arizona and India, where PVDF crystallizes in its predominant α-phase, the degree of crystallinity greatly increased. MAST and C-AST protocols were, to some extent, able to replicate the increase in crystallinity seen in PVDF after ~ 7 years in the field, but no single-stress test condition (UV, damp heat, thermal cycling) resulted in significant changes in the material properties. The MAST regimen used here was too extreme to produce realistic degradation, but the test was useful in discovering weaknesses of the particular PVDF-based outer layer structure studied. No excessive β-phase formation was observed after aging with any test condition; however, the presence of β-phase was identified locally by Fourier transform infrared spectroscopy (FTIR). We conclude that both MAST and C-AST are relevant tests for screening outdoor failure mechanisms in PVDF backsheets, as they were successful in producing material degradation that led to cracking.

Outer-layer coherent structures from the turbulent/non-turbulent interface perspective at moderate Reynolds number

This study reports the observation of outer-layer coherent structures in turbulent boundary layer from the turbulent/non-turbulent interface perspective at moderate Reynolds number. The observation relies on time-resolved particle image velocimetry measurements with a large field-of-view. The local turbulent kinetic energy is used to detect the turbulent/non-turbulent interface. In the reference frame of turbulent/non-turbulent interface, raw velocity is decomposed into three components: time average of raw velocity, conditionally averaging components and turbulent fluctuations. The triple velocity decomposition eliminates the bias of Reynolds decomposition fluctuations in the intermittent region. The conditional statistics based on the turbulent/non-turbulent interface is considered to discuss the turbulence characteristics. The basic statistics of mean raw velocity, Reynolds stresses, and swirling strength are observed in conditional statistics. And the results are compared with the results based on the traditional wall surface reference frame. Quadrant analysis is introduced to extract ejections & sweeps and reflect the statistical characteristics of the outer-layer structure. The contribution of ejections and sweeps to the Reynolds shear stresses exhibits significantly different characteristics. Then, the configuration of the turbulent fluctuations and instantaneous structures is presented relied on the uniform momentum zones in the instantaneous frames. The spatial characteristics of coherent structures are reflected by two-point correlation of the streamwise turbulent fluctuations. In addition, the spatial scale of the outer-layer structures is revealed from the turbulent/non-turbulent interface perspective.

Contamination and removal of polycyclic aromatic hydrocarbons in multilayered assemblies of firefighting protective clothing

Contaminants deposited on the firefighter protective clothing increase the risk of carcinogenesis by transdermal absorption. The effects of the inclination angle, waterproof grade, and fabric structure of the flame retardant outer layer of firefighting protective clothing on polycyclic aromatic hydrocarbon (PAH) contamination and laundering were studied by simulating smoking exposure. The permeation and transmission process of PAHs in multilayered assemblies were investigated. Results showed that the adsorption capacity of soot and PAH contamination on fabric with a water repellent finish relatively increased, and outer layer absorbed more PAHs at 180° compared to other inclination angles. The fabric structure with single layer had a strong adsorption capacity and low removal capacity for PAHs. The highest amount of PAH contaminant was found in the moisture barrier layer, and the removal rate (44.7%) was much lower than that of the outer layer cleaned through conventional laundering (91.3%). The research can better provide scientific support for pollution control and clothing cleaning in the actual firefight.

Residual Energy Maximization for Wireless Powered Mobile Edge Computing Systems With Mixed-Offloading

This paper studies a joint design of resource allocation and task offloading in a wireless powered mobile edge computing network involving different types of computation tasks. To deal with diverse computation tasks, we explore a mixed-offloading paradigm to support the coexistence of partial and binary offloading modes. Specifically, devices harvest energy from an access point (AP) via wireless power transfer (WPT) and utilize the harvested energy to execute their computation tasks using partial or binary offloading. Based on a practical non-linear energy harvesting model, a residual energy maximization problem is formulated by jointly optimizing the transmit power of the AP, the offloading power of devices, the time allocation on WPT and task offloading, and the task partitions and the binary offloading decisions of devices, which turn out to be a non-convex mixed-integer non-linear programming problem. Thus, we develop an efficient dual-layer optimization algorithm by decomposing the optimization problem into an inner and outer layer structure that aims to obtain resource allocation and offloading decisions. Simulation results show that our proposed scheme achieves residual energy gains compared to existing schemes.

Outer-layer structure arrangements based on the large-scale zero-crossings at moderate Reynolds number

The structural arrangements in the outer layer of turbulent boundary layer flows were explored with large-field time-resolved particle image velocimetry measurements at moderate Reynolds number. The large- and small-scale structures were reconstructed by the modes of multiscale proper orthogonal decomposition. The association between hairpin packets and uniform momentum zones (UMZs) was examined by the conditional averaging results based on the large-scale positive-to-negative/negative-to-positive (PN/NP) zero-crossings. The scale arrangements provided the spatial evidence that the intense small-scale swirling motions are aligned in the confined internal shear layers along the backside of the large-scale, low-speed region, which was characterized by hairpin vortex packets. The uniform momentum zones (UMZs) conditioned on the large-scale PN/NP zero-crossings were detected from the histograms of the instantaneous streamwise velocity. The attached eddy behavior was consolidated based on the conditional events, by presenting the joint probability of UMZs thickness and wall-normal location. A close agreement of the conditional averaging raw velocity and modal velocity was examined. Moreover, the conditional averaging results of the UMZs interface probability exhibited a similar spatial distribution as the small-scale turbulent kinetic energy and swirling strength, which manifests the coincidence between the hairpin heads and the UMZs interfaces. This result was confirmed by the distribution of the wall-normal locations corresponding to the maximum value of interface probability and small-scale representations, which performs a streamwise inclination angle of 15°. The statistical spatial feature demonstrated the association between hairpin packets and uniform momentum as proposed by Adrian et al. [“Vortex organization in the outer region of the turbulent boundary layer,” J. Fluid Mech. 422, 1–54 (2000)].

Improvement of solid-state anaerobic digestion of broiler farm-derived waste via fungal pretreatment

Fungus, Trichoderma longibrachiatum, was used for the pretreatment of broiler farm derived-lignocellulosic bedding material (rice husk) to enhance the subsequent solid-state anaerobic digestion (SS-AD). Fungal pretreatment efficacy was evaluated through a series of batch studies with respect to carbon-to-nitrogen (C/N) ratio and pretreatment time. Lignocellulosic outer layer structure disruption of the rice husk was prominent under the best fungal pretreatment condition evaluated (C/N ratio of 18.9 and pretreatment time of 7 days). Consequently, the resulting methane yield of 438.1 ± 20.0 NmL/gVSadded was obtained which was ~2.0-folds higher than that of the control (without pretreatment). Furthermore, in semi-continuous SS-AD, fungal pretreatment could significantly enhance digestibility of organic substance in high solid loading (30% total solids) AD process by 3.2-folds and improve microbial kinetic parameters with subsequent daily methane yield improvement by 2.4-folds. Thus, fungal pretreatment could be an environmentally-friendly and effective low-cost approach for broiler farm-derived waste management to enhance SS-AD efficacy.

Sandwich-type sheeting improved the processing and eating qualities of potato noodles.

A technology called sandwich-type sheeting was used to produce noodles with potato flakes. The technical parameters of sheeting were first optimized. Then the processing and eating qualities of potato noodles made with sandwich-type sheeting and conventional sheeting were compared. Results showed that the optimal moisture of inner-layer dough and outer-layer dough was 41 and 37%, respectively. The suitable ratio of the thickness of inner layer to that of outer layer was 3:1. The tensile strength of the sandwich-type dough sheet was 1.285 times higher than that of conventional dough sheet. The cooking loss of the sandwich-type noodles was 37.0% lower than that of conventional noodles, and the adhesiveness decreased by 51.0%. In the sandwich-type noodles, the compact gluten network structure of outer wheat layer prevented the leaching of soluble substances in the inner layer added with potato flakes, improving the cooking and eating qualities of potato noodles.

The preparation of N, S, P self-doped and oxygen functionalized porous carbon via aerophilic interface reaction for high-performance supercapacitors

Heteroatom-doped porous carbon materials are promising candidates for supercapacitor electrodes, but the simple and low-cost preparation method for heteroatom self-doped carbon, especially derived from biomass, remains a challenge. Herein, we developed a novel strategy that can provide an aerophilic interfacial reaction to produce N, S, P self-doped and oxygen functionalized porous carbon from the outer layer structure of durian peels in facile environment. The obtained porous biochar with N, S, P self-doped and abundant oxygen-containing functional groups can enhance the electrochemical capacitor performance, stability, and hydrophilicity of carbon electrode. When employed into symmetric supercapacitor device, the biochar of functionalization could deliver a specific capacitance of 158.20 F g−1 at 1 A g−1, a high energy density of 21.97 Wh kg−1 at a power density of 500 W kg−1 within a voltage window of 1.0 V and a remarkable cycling durability with 101.35% after 20,000 charge/discharge cycles at 4 A g−1 in 6 M KOH electrolyte. This work provides an effective and economic approach to obtain heteroatom self-doped and highly stable carbon-based electrode materials.

Morphology evolution of the anodized tin oxide film during early formation stages at relatively high constant potential

Nanoporous tin oxide layers were electrochemically synthesized by potentiostatic anodization of metallic tin. The current density vs time curve exists two trends in the initial stages of tin anodizing at different potentials, which are mainly attributed to the amount of oxygen released. The obtained anodic tin oxide film had an inner and outer layer structure. The morphology evolution of nanoporous tin oxide layers during anodization were studied in detail. And a model is proposed to explain the phenomenon of transverse cracks and voids formed by segmented oxide and excess oxygen, based on which an anodized tin oxide film with few cracks was prepared by suppressing the generation of oxygen.

Ru@CeO2 yolk shell nanozymes: Oxygen supply in situ enhanced dual chemotherapy combined with photothermal therapy for orthotopic/subcutaneous colorectal cancer

Hypoxia is an important factor in forming multidrug resistance, recurrence and metastasis in solid tumors. Nanozymes respond to tumor microenvironment for tumor-specific treatment is a new and effective strategy. In this study, one-pot method was used to synthesize hollow Ru@CeO2 yolk shell nanozymes (Ru@CeO2 YSNs), which possess excellent light-to-heat conversion efficiency and catalytic performance. Antitumor drug ruthenium complex (RBT) and resveratrol (Res) were dual-loaded in Ru@CeO2 YSNs, and a double outer layer structure using polyethylene glycol was constructed to form dual-drug delivery system (Ru@CeO2-RBT/Res-DPEG) that was released on demand. The double outer layer structure increased the biocompatibility of Ru@CeO2 YSNs and effectively prolong the circulation time in blood. Ru@CeO2-RBT/Res-DPEG catalyzes endogenous H2O2 to produce oxygen, which achieve in situ oxygen supply and enhanced dual-chemotherapy and photothermal therapy (PTT) for colorectal cancer. In vitro studies found that Ru@CeO2-RBT/Res-DPEG has good tumor penetration depth and antitumor effect. In addition, Ru@CeO2-RBT/Res-DPEG can alleviate tumor hypoxia, and inhibit metastasis and recurrence of orthotopic and subcutaneous colorectal cancer. Accordingly, the study shows that yolk shell nanozymes can be used as an efficient synergistic system for dual-chemotherapy and PTT to kill tumor and inhibit orthotopic colorectal cancer metastasis and recurrence.