DNA origami is a milestone in DNA nanotechnology. It is robust and efficient in constructing arbitrary two- and three-dimensional nanostructures. The shape and size of origami structures vary. To characterize them, an atomic force microscope, a transmission electron microscope, and other microscopes are utilized. However, the identification of various origami nanostructures heavily depends on the experience of researchers. In this study, we used the deep learning method (improved Yolox) to detect multiple DNA origami structures and estimate their yield. We designed a feature enhancement fusion network with the attention mechanism, and related parameters were researched. Experiments conducted to verify the proposed method showed that the detection accuracy was higher than that of other methods. This method can detect and estimate the DNA origami yield in complex environments, and the detection speed is in the millisecond range.