Structure Of Aerobic Granules Research Articles

Influence of an aniline supplement on the stability of aerobic granular sludge

In order to evaluate the stability of aerobic granules in a toxic environment, this study discussed the influence of an aniline supplement on the properties and microbial community of aerobic granules. In the early stages of sequencing batch reactor (SBR) operation, an aniline supplement slightly affected the properties of the aerobic granules (strength, growth rate, SVI and so on). This effect was thereafter removed because of a change in the microbial community and the structure of aerobic granules: with the present of aniline, microbes with biodegradation ability appeared and gathered in the aerobic granules and the aerobic granules densified and settled faster as their SVI decreased to 35 mL/g and settling velocity increased to 41.56 m/h. When a synthetic waste water containing acetate as carbon source was used as influent, aniline (10–500 mg/L) could be degraded in 6 h, at a rate as high as 37.5 mg aniline/(L·h), with a removal rate in excess of 90%, while the effluent COD fell below 100 mg/L from the initial about 2000 mg/L. The aerobic granules cultured by acetate were compact, stable and resistant to aniline.

Molecular mechanisms governing aerobic granular sludge processes

The aerobic granular sludge process is a promising technology for wastewater treatment. The formation and structure of aerobic granules are traditionally thought to depend highly on selection pressures, while the underlying molecular mechanisms are unclear. It is well known that bacteria coordinate their behavior using small signaling molecules, known as quorum sensing (QS). This paper is an attempt to provide updated information on QS mechanisms governing granular sludge processes. It is shown that QS-mediated cellular communication has a significant role throughout aerobic granulation, including granule development, structural stability and integrity maintenance. Such understanding is helpful for developing novel aerobic granular sludge processes.

Multiple EPS interactions involved in the cohesion and structure of aerobic granules

This study aims to clarify the biochemical nature and interactions of Extracellular Polymeric Substances (EPS) involved in the structure and cohesive properties of aerobic granules. Granules were incubated with selective hydrolytic enzymes or with chemicals and the resistance of digested granules to shear stress was evaluated. After α-amylase digestion, the hydrodynamic stress released macro-particles (>315μm) while soluble molecules (<1.5μm) and micro-particles (1.5–315μm) where mainly recovered after savinase and EDTA treatments. These data show that α (1–4) glucans and proteins are key polymers for granule cohesion and that divalent cationic bridging is a major aggregative mechanism.On the basis of these experiments and microscopy observations, a model is proposed for the spatial organization of EPS in the granular structure, in which α glucans are arranged in a capsular layer surrounding bacterial clusters while anionic proteins constitute the intercellular cement that may reinforce cohesion inside the bacterial clusters.

A review on the essential role of substrate on aerobic granulation

Aerobic granulation is a selection pressure-driven cell-to-cell immobilisation process. So far, the essential role of substrates on aerobic granulation has not been well clarified. This paper reviews current studies about aerobic granulation with different substrates. Although substrates do not exert a major selection pressure on aerobic granulation, they will select substrate-related microorganisms and adjust microbial diversity, and further influence the formation, stability, and structure of aerobic granules. Co-substrate cultivation strategy can be adopted to accelerate aerobic granulation and improve the stability and performance of aerobic granules. This paper hopes to provide a platform for developing full-scale aerobic granules based bioreactors.

Bioaugmentation and coexistence of two functionally similar bacterial strains in aerobic granules

The survival of the inoculated microbial culture is critical for successful bioaugmentation but impossible to predict precisely. As an alternative strategy, bioaugmentation of a group of microorganisms may improve reliability of bioaugmentation. This study evaluated simultaneous bioaugmentation of two functionally similar bacterial strains in aerobic granules. The two strains, Pandoraea sp. PG-01 and Rhodococcus erythropolis PG-03, showed high phenol degradation and growth rates in phenol medium, but they were characterized as having a poor aggregation activity and weak bioflocculant-producing and biofilm-forming abilities. In the spatially homogeneous batch conditions, strain PG-01 with higher growth rates outcompeted strain PG-03. However, the two strains could stably coexist in the spatially heterogeneous conditions. Then the two strains were mixed and bioaugmented into activated sludge in two sequencing batch reactors, which were operated with the different settling times of 5 and 30 min, respectively. Aerobic granules were developed only in the reactor with a settling time of 5 min. Fluorescence in situ hybridization and denaturing gradient gel electrophoresis showed that the two strains could coexist in aerobic granules but not in activated sludge. These findings suggested that the compact structure of aerobic granules provided spatial isolation for coexistence of competitively superior and inferior strains with similar functions.

The effect of upflow air velocity on the structure of aerobic granules cultivated in a sequencing batch reactor

The effect of upflow air velocity on the formation and structure of aerobic granules was studied in three column sequencing batch reactors. Upflow aeration would be the major cause of hydrodynamic shear force in the column reactor. Results showed that high upflow air velocity resulted in more compact, denser, rounder, stronger and smaller aerobic granules, while high biomass retention in the reactor was achieved. It was found that high upflow air velocity could induce granular sludge to secrete more cell polysaccharides which in turn contributed to the compact and strong structure. It appears from this study that the structure of aerobic granules could be controlled by manipulating the upflow air velocity.