Wood and steel have different physical properties. By combining the two kinds of materials as bonded parallel beams, wood-steel frame structures can be prepared. This work considers the seismic performance of such engineering structures. Based on the Rayleigh damping model of substructures, the non-proportional damping coefficient is used to quantify the structural non-proportional damping characteristic of wood-steel frame structures. A complex mode superposition method is used to calculate seismic responses. Numerical cases showed that compared with the frame structure with upper steel and lower wood, the overall lateral performance is lower and the local lateral performance is higher for the frame structure with upper wood and lower steel. The overall lateral seismic design needs to be improved for the wood-steel frame structure with upper wood and lower steel. The local lateral seismic design needs be improved for the wood-steel frame structure with upper steel and lower wood.