Strontium Isotope Analysis Research Articles

Are you those folks from across the sea? Biochemical analysis of human remains from the Roman Iron Age site in Grzybnica (Poland)

Abstract Stone circles of the Wielbark culture, such as those found in Grzybnica, have long fascinated researchers, who debate their function and significance. The aim of the presented study is to determine the origins and subsistence strategies of the population buried near the stone circles in Grzybnica, which may help highlight the role of these sites within Wielbark communities. For analysis, human remains from the cemetery in Grzybnica, West Pomeranian Voivodeship, Poland, were utilized. Radiocarbon dating of five samples, stable isotope analysis of carbon and nitrogen in two samples, and strontium isotope analysis in 20 cremated human remains and 12 plant samples were conducted. Radiocarbon dating confirmed the earlier dating of the site to the period between approximately 80 and 260 CE. Dietary studies indicated that the individuals buried here regularly consumed millet (mean δ13C = –17.8) and animal protein (mean δ15N = 9.5). This diet corresponds with the variability described for the Wielbark culture site in Rogowo. The strontium isotope analysis revealed significant variation between the river valley (0.7157–0.7264) and the adjacent upland (0.7109–0.7138). The isotopic values in humans were homogeneous, ranging from 0.7113 to 0.7130, indicating no outliers that might suggest the presence of migrants. The variability observed in humans corresponds with the values observed on the upland. The results suggest that the group of individuals buried in Grzybnica was a small community that sourced their food from the upland. It is possible that this community was involved in the care of the stone circles, and the circles themselves may have been places of gatherings for local communities. However, it should be noted that only about 20 % of the graves found at the site were analyzed. The lack of migrants may be due to the low mobility of this group or methodological limitations related to the post-glacial nature of the geological substrate. Alternatively, it could also be due to the limitations associated with studying cremated remains, which allow for the reconstruction of events from only about 10 years prior.

Making connections: ostrich eggshell beads as indicators of precolonial societal interaction in southeastern southern Africa

ABSTRACT Ostriches are peculiar birds and their strangeness has been recognised by southern African hunter-gatherers through multiple symbolic associations. Such qualities are likely to have been enhanced where people had access to beads made from their eggshell but did not have direct knowledge of the birds themselves. Southeastern southern Africa, encompassing the Maloti-Drakensberg Mountains, the eastern half of the Eastern Cape Province and much of KwaZulu-Natal is one such area. We review the unusual attributes and associations of ostriches and their eggshell before briefly summarising previous work that has used strontium isotope analysis to investigate past bead exchange networks in this region. Because bead size can be controlled and patently varies through time and space, we then employ it as another potential indicator of the existence and spatial extent of past social networks. Having critically considered previous efforts in this field, we report on our work to build the largest sample of such data yet obtained in southern Africa and compare our preliminary results with other signals of interaction between precolonial hunter-gatherer and (where applicable) agropastoralist communities.

Provenance of mineral dust deposited on Antarctica over the last sixty years by strontium isotopic analysis of snow from Dome C

The strontium (Sr) concentration and 87Sr/86Sr isotope ratio have been determined in 132 snow pit samples collected at Dome C, on the East Antarctic Plateau, corresponding to the period 1958–2019, and in 12 surface snow samples collected at the same site in 2016–2017. The average Sr concentration was 8.1 ± 5.7 pg g−1 (mean ± SD) in snow pit samples and 10.1 ± 8.0 pg g−1 (mean ± SD) in surface snow, without any significant temporal trend. The 87Sr/86Sr isotope ratio showed small variations (<0.15%), with an average 87Sr/86Sr value of 0.7094 ± 0.0010 (mean ± SD) in snow pit and 0.7103 ± 0.0004 (mean ± SD) in surface snow samples. These results seem to suggest that no change in the source(s) of mineral dust has occurred throughout the period investigated.Comparison of the 87Sr/86Sr isotope ratio of the snow samples with those of potential source areas suggests that the mineral dust reaching the East Antarctic plateau over the last decades is of a mixed origin, with a contribution from Patagonia and one or more other source(s) having a more radiogenic Sr isotopic signature, with a major fraction coming from South Australia.

Freshwater exploitation at Ajvide - Pitted ware culture fishing practises investigated through laser ablation facilitated strontium isotope analyses

The importance of marine resources for the Neolithic hunter-fisher-gathers of the Pitted Ware Culture of Gotland, Sweden, is well documented through zooarchaeological analyses and diet studies of human remains. Terrestrial areas were important for living and supplementing the diet but the extent of the terrestrial territories and regions of land use for different groups is largely unknown. The presence of euryhaline species in recovered zooarchaeological assemblages indicates that freshwater fishing or fishing in the brackish estuaries of the Baltic Sea was part of the subsistence practises. To explore if the inland freshwaters of Gotland were used and, if exploited, where they were located, 18 teeth from euryhaline fish from the Pitted Ware Culture site Ajvide on Gotland were selected. The 87Sr/86Sr isotope ratios in the fish teeth were analysed using laser ablation-multi collector-inductively coupled plasma-mass spectrometry and correlated with an updated bioavailable baseline of Gotlandic water sources. Through this approach, the habitational origin of the fish was shown to primarily stem from at least six freshwater sources located in the west-central area of Gotland, in close relation to the site, with a few individuals originating from within the Baltic Sea. The study highlights the significance of ichthyoarchaeological analysis in understanding the territorial practice of past foraging societies and recommends further studies on euryhaline species to expand our knowledge of fish habitat, human resource utilization and land use.

Strontium Isotope Analysis at an 18th Century Burial Ground in Semonice (Czech Republic) – the Use of Modern Skeletal Remains and its Narrative and Interpretative Value

Strontium Isotope Analysis at an 18th Century Burial Ground in Semonice (Czech Republic) – the Use of Modern Skeletal Remains and its Narrative and Interpretative Value

Strontium isotopes and cremation: Investigating mobility patterns in the Roman city of Mutina (north-eastern Italy)

Cremation was a very common ritual in ancient Roman funerary traditions. However, the study of cremated human remains has always been complex and challenging, which has often led to an imbalance in data recording between inhumations and cremations. In this work, we study 14 cremation burials from two different urban cemeteries in the Roman city of Mutina (Modena, Emilia-Romagna, north-eastern Italy). The use of strontium isotope analysis provides insights into the mobility pattern and provenance of individuals cremated and buried at Mutina. The isotopic results suggest that nine samples fall outside the local bioavailable strontium range of the city of Modena, given their different 87Sr/86Sr values compared to the ratio compatible with alluvial deposits in the Po Valley. Both the isotopic results and the manufacture of some funerary objects suggest that the probable provenance of some individuals is compatible with western (Pre)Alpine areas. The values of 87Sr/86Sr also complement the results obtained from the osteological analysis increasing the minimum number of individuals buried in at least one grave. Our study revealed key insights about cremated individuals from Italy, highlighting variations of the mobility patterns within Roman funerary contexts of Mutina.

Improved precision at high-spatial resolution of strontium isotope analysis in apatite and apatite inclusions by LA-MC-ICP-MS with 1013 Ω amplifiers

Recent advancements in in situ analytical techniques have generated new interest in measuring strontium (Sr) isotopes in rock-forming apatite and apatite inclusions in zircon, because they can bring new insights into the primary signatures of magmatic sources. This paper introduces a novel approach based on laser ablation multi-collector inductively coupled plasma mass spectrometry (LA-MC-ICP-MS), which allows to achieve higher precision in situ analysis of Sr isotopes in small volumes of samples. The use of 1013 Ω current amplifiers for the acquisition of the signal on m/z 83, 83.5, 84, 85, 85.5, 86, 86.5, 87 and 88, along with a specific data reduction protocol, enhance both the internal precision and the external reproducibility of the 87Sr/86Sr ratio by a factor of four, presenting a significant improvement over the conventional use of 1011 Ω amplifiers. An internal precision better than 0.45‰ (i.e. 450 ppm; 2 s.e.) on the 87Sr/86Sr ratio can be achieved on the Durango apatite standard with a 13 μm square-shaped laser beam and a laser ablation pit of 60 μm depth. With a 5 μm beam, the precision is 2.2‰ on average with a 10 μm ablation pit depth for this standard. This analytical procedure was applied to investigate Sr isotopes in apatite and apatite inclusions within zircons from seven high BaSr magmatic rocks in the Northern Highland Terrane, Scotland. For these well-characterised, geochemically undisturbed samples, the data reveals similar Sr isotope compositions between apatite inclusions armoured in zircon, matrix apatites, and the bulk rock. This highlights the ability of apatite inclusions to faithfully record primary signatures of host magmas, elucidating their utility as reliable indicators in petrological studies of ancient samples.

Well supplied in life, set aside in death: A multi-isotope study of Justinian plague victims from Saint-Doulchard (France, 7th-8th centuries AD).

Justinian plague and its subsequent outbreaks were major events influencing Early Medieval Europe. One of the affected communities was the population of Saint-Doulchard in France, where plague victim burials were concentrated in a cemetery enclosure ditch. This study aimed to obtain more information about their life-histories using the tools of isotope analysis. Dietary analysis using carbon and nitrogen isotopes was conducted on 97 individuals buried at Le Pressoir in Saint-Doulchard, with 36 of those originating from the enclosure ditch. This sample set includes all individuals analyzed for plague DNA in a previous study. Mobility analysis using strontium isotope analysis supplements the dietary study, with 47 analyzed humans. The results are supported by a reference sample set of 31 animal specimens for dietary analysis and 9 for mobility analysis. The dietary analysis results showed significantly different dietary behavior in individuals from the ditch burials, with better access to higher quality foods richer in animal protein. 87Sr/86Sr ratios are similar for both studied groups and indicate a shared or similar area of origin. The results suggest that the ditch burials contain an urban population from the nearby city of Bourges, which overall had a better diet than the rural population from Saint-Doulchard. It is implied that city's population might have been subjected to high mortality rates during the plague outbreak(s), which led to their interment in nearby rural cemeteries.

The Relevance of Sr–O–C Isotope Analysis on Burnt Human Skeletal Remains in Archeological and Forensic Contexts: A Review and Future Directions

AbstractRecovery and identification of heavily burnt and fragmented skeletal remains pose significant challenges in fire and arson investigations. The ability to identify individuals is crucial in such cases, prompting extensive research into various techniques to aid in the identification process, including isotopic analysis. Although direct personal identification is not achievable through isotopic analysis of skeletal remains, it serves as a valuable tool for obtaining chemical elements of the biological profile. Strontium (Sr), oxygen (O), and carbon (C) isotope analyses have been widely used in archeological contexts but are relatively new approaches in the medicolegal domain. Oxygen isotope analysis, for instance, offers insights into the burning temperature, while strontium isotope analysis provides valuable information for tracing the individual's movement across geologically distinct landscapes. Consequently, the investigation of the impact of heat on the biogenic isotope composition of human bones in forensic contexts has gained significant attention. This article presents a comprehensive overview of the key archeological and forensic questions addressed by Sr–O–C isotope analysis of burnt skeletal remains. By examining the current state of the art and prospects, we aim to contribute to a broader understanding and potential advancements in the field of forensic isotope research.This article is categorized under: Forensic Biology &gt; Interpretation of Biological Evidence Forensic Anthropology &gt; Trauma Analysis

Biomolecular evidence reveals mares and long-distance imported horses sacrificed by the last pagans in temperate Europe.

Horse sacrifice and deposition are enigmatic features of funerary rituals identified across prehistoric Europe that persisted in the eastern Baltic. Genetic and isotopic analysis of horses in Balt cemeteries [1st to 13th centuries CE (Common Era)] dismantle prevailing narratives that locally procured stallions were exclusively selected. Strontium isotope analysis provides direct evidence for long-distance (~300 to 1500 kilometers) maritime transport of Fennoscandian horses to the eastern Baltic in the Late Viking Age (11th to 13th centuries CE). Genetic analysis proves that horses of both sexes were sacrificed with 34% identified as mares. Results transform the understanding of selection criteria, disprove sex-based selection, and elevate prestige value as a more crucial factor. These findings also provide evidence that the continued interaction between pagans and their newly Christianized neighbors sustained the performance of funerary horse sacrifice until the medieval transition. We also present a reference 87Sr/86Sr isoscape for the southeastern Baltic, releasing the potential of future mobility studies in the region.

Unveiling Hunnic legacy: Decoding elite presence in Poland through a unique child’s burial with modified cranium

This article presents a double burial from Czulice indicating elements of the Hunnic culture. Individual I, aged 7–9, and Individual II, aged 8–9 with a skull deformation, were both genetically identified as boys. Individual II, who exhibited genetic affinity to present day Asian populations, was equipped with gold and silver items. In contrast, Individual I displayed European ancestry. The application of strontium isotope analysis shed light on the origins of the individuals. Individual I was non-local, while Individual II was identified as a local, but also falling within the range commonly associated with the Pannonian Plain. Stable isotope analysis suggested a diet consisting of inland resources. Through radiocarbon dating, this burial was determined to date back to the years 395–418 CE, making it the earliest grave of its kind discovered in Poland. The analyses have provided new insights into the nature of the relationship between the Huns and the local inhabitants.

Preliminary strontium isotope (87Sr/86Sr) baselines for the Bjäre Peninsula and Halland in southern Sweden

During the last decade, the application of strontium isotope analysis (87Sr/86Sr) has increased rapidly. The understanding of the strontium isoscape and the construction of a strontium isotope baseline in southern Scandinavia are biased toward Denmark and southwestern to eastern Scania. We report the results of new baseline samples in Halland and the Bjäre Peninsula, adding to the rich strontium isotope library that exists for southern Scandinavia. We add nuance to the previous divisions of Scania, in which the Bjäre Peninsula is not well represented. The results show that the Bjäre Peninsula is characterized by relatively low 87Sr/86Sr values gathered in the preliminary baseline 0.7100 ± 0.0024 (2σ, n = 8), similar to that of southwest Scania and Zealand in Denmark. There is a greater variation and higher values among the Halland samples. Together with previously published faunal samples, a preliminary 87Sr/86Sr baseline for Halland is 0.7122 ± 0.0055 (2σ, n = 24). We apply these baseline results to archaeological cases, both human and animal, from recent excavations in the Bjäre Peninsula. The results imply that there was a certain degree of mobility and interaction across the landscape in this region of southern Scandinavia in prehistory.

Advances and prerequisites for strontium isotope analyses through laser ablation in an aquatic context – Targeting Mid-Neolithic Baltic Sea harp seal (Pagophilus groenlandicus) mobility and breeding grounds

Harp seals (Phoca groenlandicus), once present in the Baltic Sea, now stand extinct. During the Middle Neolithic period, they held significant dietary importance for the Pitted Ware Culture hunter-fisher-gatherers in Scandinavia. Because they are no longer available for ecological studies, little is known about their specific behavioural intricacies, such as diet, mobility, and reproductive strategies. Because of the seal's pivotal role in these human societies and because they provide an interesting ecological case study on post-isolation ecological adaptation to new environmental conditions, a comprehensive investigation into Baltic Sea harp seal behaviour is warranted.In this pursuit, we employ sequential analysis of strontium isotope ratios (87Sr/86Sr) in harp seal teeth sourced from archaeological contexts across three study regions. This data is harmonized with osteometric analyses from fifteen archaeological sites from the Baltic Sea area, establishing a methodological framework for comprehending the Sr isotopic pathway in a brackish water inland sea. By adopting this approach, we unveil breeding and mobility patterns of the long-extinct Baltic Sea harp seals, approximate specific breeding areas connected to each group of seals and delve into the ontogenic temporal frame governing these observed patterns. This endeavour culminates in an enriched understanding of the adaptive behaviours exhibited by Baltic Sea harp seals and equips us with the insights necessary to decipher the lifestyle of the human societies intricately intertwined with them.

Isotopic biographies reveal horse rearing and trading networks in medieval London.

This paper reports a high-resolution isotopic study of medieval horse mobility, revealing their origins and in-life mobility both regionally and internationally. The animals were found in an unusual horse cemetery site found within the City of Westminster, London, England. Enamel strontium, oxygen, and carbon isotope analysis of 15 individuals provides information about likely place of birth, diet, and mobility during the first approximately 5 years of life. Results show that at least seven horses originated outside of Britain in relatively cold climates, potentially in Scandinavia or the Western Alps. Ancient DNA sexing data indicate no consistent sex-specific mobility patterning, although three of the five females came from exceptionally highly radiogenic regions. Another female with low mobility is suggested to be a sedentary broodmare. Our results provide direct and unprecedented evidence for a variety of horse movement and trading practices in the Middle Ages and highlight the importance of international trade in securing high-quality horses for medieval London elites.

Patrilocality at the Beginning of Farming? An Isotopic Approach from SE Moravia

This research combines Strontium (87Sr/86Sr) and Oxygen (δ18O) isotope analysis to challenge the prevailing interpretation of patrilocal exogamic practices among eastern European Early Neolithic Linearbandkeramik (LBK) communities. Patrilocality has been considered the key factor influencing the mobility patterns of central Europe’s first farmers (c. 5500–4900 cal. BC), especially in the south-eastern Moravian region (Czech Republic). Focusing our attention on both male and female tooth enamel samples from cemeteries, settlement graves and small clusters of graves, this paper reassesses the correlation between mobility, biological sex, and funerary practices. This task is accomplished by establishing a new isotopic footprint using new 87Sr/86Sr data, as well as significantly increasing the number of sampled individuals for 87Sr/86Sr and δ18O. The outcome of this research contributes to a better understanding of the mobility patterns among early farmers in central Europe, challenging existing theories and providing new insights into their social and cultural dynamics.

Formation processes of groundwater in a non-ferrous metal mining city of China: Insights from hydrochemical and strontium isotope analyses.

Tongling is a significant non-ferrous metal mining city in China, which produces waste that negatively impacts the area's water environment. It is essential to comprehend the hydrochemical properties and formation processes of groundwater to safeguard and utilize it efficiently. We explored major ions, strontium, and its isotopes in water and river-bottom samples from the northern (i.e., A-A' section) and southern (i.e., B-B' section) areas. The hydrochemical facies show the mining activities have a greater impact on surface water than on groundwater. Groundwater hydrochemical formation results from several factors, with water-rock interaction and ion exchange being primary. Additionally, the dissolution of calcite, dolomite, and feldspar, oxidation of pyrite, and hydrolysis of carbonate minerals also impact the formation of groundwater chemistry. Our analysis of strontium and its isotopes indicates that carbonate dissolution primarily occurred in the recharge area; the runoff from the recharge to the discharge area results in the dissolution of certain silicate rocks; calcite dissolution sources account for > 70% contribution in both surface water and groundwater water-rock interactions, whereas silicate rock dissolution sources and dolomite dissolution sources account for < 30%. Due to changed order of dissolved carbonate and silicate minerals during groundwater flow, the distribution of strontium and its isotopes in the A-A' section is opposite to that in the B-B' section. The findings provide a basis for developing, utilizing, managing, and protecting groundwater resources, especially in similar mining areas.

Life-history of Palaeoloxodon antiquus reveals Middle Pleistocene glacial refugium in the Megalopolis basin, Greece

The Balkans are considered a major glacial refugium where flora and fauna survived glacial periods and repopulated the rest of Europe during interglacials. While it is also thought to have harboured Pleistocene human populations, evidence linking human activity, paleoenvironmental indicators and a secure temporal placement to glacial periods is scant. Here, we present the first intra-tooth multi-isotope analysis for the European straight-tusked elephant Palaeoloxodon antiquus, on an adult male individual excavated in association with lithic artefacts at the MIS 12 site Marathousa 1 (Megalopolis basin, Greece). The studied find also exhibits anthropogenic modifications, providing direct evidence of hominin presence. We employed strontium, carbon and oxygen isotope analysis on enamel bioapatite to investigate its foraging and mobility behaviour, using a sequential sampling strategy along the tooth growth axis of the third upper molar, to assess ecological changes during the last decade of life. We found a geographically restricted range, in a C3-dominated open woodland environment, and relatively stable conditions over the examined timeframe. Our results show that, despite the severity of the MIS 12 glacial, the Megalopolis basin sustained a mesic habitat, sufficient plant cover and limited seasonal fluctuations in resource availability, pointing to its role as a glacial refugium for both fauna and hominins.

Isotopic Evidence for Mobility in the Copper and Bronze Age Cemetery of Humanejos (Parla, Madrid): a Diachronic Approach Using Biological and Archaeological Variables

Over the last several decades, the application of aDNA and strontium isotope analyses on archaeologically recovered human remains has provided new avenues for the investigation of mobility in past societies. Data on human mobility can be valuable in the reconstruction of prehistoric residential patterns and kinship systems, which are at the center of human social organization and vary across time and space. In this paper, we aim to contribute to our understanding of mobility, residence, and kinship patterns in late Prehistoric Iberia (c. 3300–1400BC) by providing new strontium data on 44 individuals from the site of Humanejos (Parla, Madrid). The study presented here is multi-proxy and looks at these new data by interweaving biological, chronological, and archaeological information. This analysis found that 7/44 individuals buried at Humanejos could be identified as non-local to the necropolis. Although more men (n = 5) than women (n = 2) were found in the non-local category, and more non-local individuals were identified in the pre-Bell Beaker (n = 5) than in Bell Beaker (n = 1) or Bronze Age (n = 1), we find no statistically significant differences concerning sex or time period. This contrasts with other archaeological datasets for late prehistoric Europe which suggest higher female mobility, female exogamy, and male-centered residential patterns were common. At Humanejos, we have also identified one non-local female whose exceptional Beaker grave goods suggest she was an individual of special status, leading to additional questions about the relationships between gender, mobility, and social position in this region and time period.

A tale of two islands: tectonic and orbital controls on marine terrace reoccupation, Channel Islands National Park, California, USA

AbstractIn areas of low uplift rate on the Pacific Coast of North America, reoccupation of emergent marine terraces by later high sea‐stands has been hypothesised to explain the existence of thermally anomalous fauna (mixtures of warm and cool species) of last interglacial age. If uplift rates have been low for much of the Quaternary, it follows that higher (older) terraces should also show evidence of reoccupation. Strontium isotope analyses of fossils from a high‐elevation marine terrace on Anacapa Island, California, yield ages ranging from ~2.4–2.3 Ma to ~1.4–1.5 Ma. These results indicate that terrace reoccupation and fossil mixing on Anacapa Island could have taken place over several interglacial periods in the early Pleistocene. Terrace reoccupation over this time period is likely a function of both a low uplift rate and the timing of orbital forcing of glacial–interglacial cycles. Climate change in the early Pleistocene was modulated by the 41 ka obliquity cycle, and glacial–interglacial cycles were much shorter than later in the Pleistocene. Nearby San Miguel Island also has evidence of terrace reoccupation, with Sr isotope ages of shells from several high‐elevation terraces ranging from ~1.21–1.25 Ma to ~0.43–0.50 Ma. However, the frequency of terrace reoccupation was lower than on Anacapa Island. The uplift rate of San Miguel Island is higher than that of Anacapa Island and terraces formed when glacial–interglacial cycles were longer. The frequency of marine terrace reoccupation is controlled by the rate of tectonic uplift and the timing of orbital forcing of sea level change during glacial–interglacial cycles.

Isotopic Insights into Livestock Production in Roman Italy: Diet, Seasonality, and Mobility on an Imperial Estate

ABSTRACTAgriculture is the most important intersection between farming communities and the natural world, with major implications for land exploitation and labour organisation. In Italy, at the heart of the Roman Empire, understanding of agriculture remains heavily dependent on ancient sources, which are unable to provide a regional or diachronic view of practices across the socio-economic spectrum. In order to gain insight into agricultural economies in Roman Italy and their social and environmental implications, this article reconstructs agro-pastoral strategies at an imperial estate in southern Italy through a multi-isotope investigation of livestock bone collagen and tooth enamel. Analysis of carbon, nitrogen, oxygen and strontium isotopes (δ13C, δ15N, δ18O, 87Sr/86Sr) are combined to evaluate animal management and mobility at Vagnari vicus and the villa of San Felice in the Basentello Valley. Results reveal taxon-specific herding strategies with the potential for significant inputs from legume forage/fodder and/or natural environments. Caprine herding did not appear to include long-distance transhumance. This analysis moves past previous text-based generalisations to provide a new and nuanced perspective on animal production in rural southern Italy and its economic and environmental implications.