Strong Positive Correlation Research Articles

Utility of Shear Wave Elastography for the Diagnosis of Plantar Fasciitis: Comparison Between Symptomatic and Asymptomatic Sides in Unilaterally Affected Patients.

Plantar fasciitis (PF) can cause pain in the heel, which can affect everyday activities. While it often resolves on its own, diagnosing PF to rule out other hind foot conditions by imaging modality in cases of recurrence can be difficult. Methods such as MRI and ultrasonography are helpful, but the use of elastography, specifically shear wave elastography (SWE), as a tool for diagnosing PF is being studied. This comparative observational studyincluded patients over 18 years presenting with unilateral hind foot pain who were investigated using SWE. Exclusions comprised those who were bilaterally affected and with foot deformities, trauma history, or prior injection therapy. Patients' AOFAS Ankle-Hindfoot Scores were assessed along with visual analog scale (VAS) scores, followed by SWE examination of both heels. The study found no significant difference in the plantar fascia thickness between affected and unaffected sides, with a mean thicknessof 4.3±0.8mm and 5.1±0.6mm, respectively. Shear wave velocity (SWV) was lower on the affected side, indicating reduced stiffness compared to the unaffected side. The Spearman rank test revealed strong direct correlations between SWV and both the VASand HF-AOFAS scores on the affected side. The study observed that SWEenhances B-mode ultrasonography in detecting early PFeven with normal plantar fascia thickness, offering a user-independent and reliable tool for treatment monitoring and correlation with functional and pain scores. Further research with larger populations can aid in developing a clinico-radiological classification system for PF, improving prognostication and treatment guidance.

The Impact of Medical Physical Training and a Structured Personalized Exercise Training Program on Hemodynamic Parameters and Arterial Stiffness in Pregnant Women.

In developed countries, heart disease is the primary cause of maternal mortality during pregnancy. Arterial stiffness, an independent risk factor for atherosclerosis and a predictor of cardiovascular complications, can be assessed using the augmentation index (AIx) and pulse wave velocity (PWV). In this prospective study, we aimed to evaluate diverse hemodynamic parameters and arterial stiffness in pregnant women before and after participating in a structured, personalized exercise training program. Forty healthy pregnant women, non-smokers, who agreed to participate daily for 12 weeks in a physical exercise training program under the supervision of a team made up of an obstetrician, a cardiologist, and a physiotherapist were included. Anthropometric characteristics, arterial function, and physical activity data were collected from the participants at two different time points: at the beginning of the exercise training program (T0) and at the end, after 12 weeks (T1). Upon conducting a statistical analysis, it was discovered that there were noteworthy disparities (p = 0.05) in body mass index, brachial AIx, systolic blood pressure, and pulse pressure values between the two time points. The regression analysis for the AIx brachial values and the PWVao values from Trim II (T0) and Trim III (T1) showed major differences between these two time points; the association between the AIx brachial values in the second and third trimesters of pregnancy revealed a strong direct significant correlation (p < 0.001), and the correlation between the PWVao values in the second (T0) and third trimester (T1) of pregnancy was weak and insignificant (p = 0.12). The findings of our study indicate that a personalized exercise training program positively impacts the physical and psychological well-being of pregnant women, leading to a reduction in PWV.

Productivity and nutritional value of perennial cereal grasses in the Middle Cis-Urals

The purpose of the research was the assessment of feed productivity and nutritional value of 13 new varieties of meadow timothy, meadow fescue and awnless brome when cultivated for green mass in the Middle Cis-Urals. The research was carried out in 2018–2022 on sod-podzolic medium loamy soil of the Udmurt Republic. Meteorological conditions of the growing seasons during the years of the research were different: 2018, 2021 and 2022 were arid (hydrothermal coefficient – 0.89, 0.78 and 0.91, respectively), 2020 – slightly arid (HTC – 1.04), 2019 – waterlogged (HTC – 1.73). The beginning of regrowth of the studied varieties of perennial cereal grasses was noted in the third ten days of April; the grass reached mowing ripeness in 44–60 days. The yield of meadow timothy varieties on average for three years of using the grass stand was at the level of 3.5–3.9 t/ha of dry weight, meadow fescue – 2.3–2.4 t/ha, awnless brome – 4.5–4.7 t/ha. The “year conditions” factor had a primary influence on the variation in yield, density and height of the grass stand – 92.4…97.4 %, 62.9…79.2, 80.3…82.6 %, respectively. The “genotype x year” factor also influenced the number of shoots and their height. Fescue varieties were distinguished by a relatively high content of crude fat (2.5–3.1 %), sugars (15.6–17.7 %), and calcium (0.31–0.36 %). High content of crude fat (2.3–2.6 %), phosphorus (0.68–0.71 %), potassium (2.45–2.52 %), calcium (0.31–0.39 %) noted in plant samples of awnless brome varieties. A positive strong correlation between the yield of dry mass of cereal grasses and the content of phosphorus (r = 0.87…0.96) and potassium (r = 0.69…0.85) in it was revealed. Some varieties of perennial cereal grasses were distinguished by their nutritional value: meadow timothy varieties Sleipnir (Finland), Aturo (Germany) and Tamiza (Germany) with a yield per 1 hectare of metabolic energy of 32.3–34.4 GJ, digestible protein – 0.14–0.16 tons, feed units – 2.41–2.60 thousand; meadow fescue variety Karpatchi (Netherlands) – 21.2 GJ, 0.09 tons, 1.57 thousand; awnless brome variety Gvardeets (Russia) – 35.7 GJ, 0.25 tons and 2.58 thousand, respectively. Cultivation of Karpatchi fescue, Sleipnir timothy and Gvardeets brome is energetically and economically profitable: energy efficiency coefficient – 1.9; 3.0 and 3.6, profitability level – 65; 101 and 161 %, respectively.

A bibliometric analysis of publications on special education between 2011 and 2020

AbstractThe present study aims to identify the most productive countries, journals, authors, institutions and the most used keywords in the field of special education during 2011–2020, based on the WoS database. The widespread effects of the papers and how they are related were analyzed with the bibliometric analysis method. The findings of the study showed that the USA is inarguably the most productive country, followed by England and Australia. On the other hand, there was a very strong positive correlation(r = 0.929) between the number of papers published by countries and their h-index, a similar finding was also found to be present between the countries' h-index and GDP per capita (r = 0.790). Moreover, it was found that the journals with the highest quartile (Q1 and Q2) in the field of special education published significantly more papers than the journals with the lowest quartile (Q3 and Q4). Matson, JL (USA), Sigafoos, J (New Zealand) and Lancioni, GE (Italy) were determined as the most prolific authors, respectively. Autism, intellectual disability, and Down syndrome were the phrases most frequently used as keywords. Our findings provide key information regarding the developments that the research direction of special education field has recently taken. This study also serves a potential roadmap for future studies.

Vegetation–Topographic Landscape and the Influence of Water and Sediment in the Shule River Basin

The study of the effect of vegetation cover on water and sediment content is of great significance for the in-depth understanding of ecological and environmental effects in river basins and the formulation of corresponding management measures. Based on the monitoring data of rainfall and runoff of Panjiazhuang and Dangchengwan Hydrologic Stations in Shule River Basin from 2000 to 2020 and the sediment discharge of Changmabao, methods such as geographic information technology (GIS), landscape pattern analysis, land use transfer matrix, correlation analysis, principal component analysis, and linear regression analysis were used to study water and sediment change, land use pattern, vegetation change characteristics, and local water and sediment change in Shule River Basin and construct vegetation–topographic landscape factors. The main research results are as follows: (1) Vegetation coverage in the Shule River Basin increased year by year from 2000 to 2020, with a cumulative increase of 0.064 in 20 years. Vegetation cover has a significant effect on water and sediment content, and the correlation is −0.966. (2) The cultivated land area of the Shule River Basin increased by 604 km2 from 2000 to 2020, and the conversion rate was 67%. From 2000 to 2020, the water area increased by 442 km2, and the conversion rate was 51%. The area of grassland and forest increased by 198 km2 and 12 km2, respectively, and the conversion rate was 68% and 33%, respectively. Forest had the highest transfer rate (0.67). The lowest conversion rate was 0.32 for grassland. (3) The variation coefficient of water and sediment content in Shule River Basin during 1971–2020 was 45.21%, and the highest variation coefficient during 2001–2010 was 49.15%. The lowest variation coefficient was 39.73% during 2011–2020. The annual sediment transport in the Shule River Basin fluctuates greatly and has a high degree of dispersion during 1971–2020. (4) The results of the landscape index in Shule River Basin during 2000–2020 had a small difference, with a difference of less than 0.5. According to the principal component analysis of landscape index and water and sediment content, the maximum patch index (LPI) had the strongest positive correlation with water and sediment content (0.958). The diversity index SHDI had the strongest negative correlation with water and sediment content (−0.995).

BASIC INDICATORS OF PHYSICAL DEVELOPMENT IN CHILDREN AGED 5-6 YEARS WITH RECURRENT RESPIRATORY INFECTIONS

Background. Recently, scientific publications on various aspects of the problem of recurrent respiratory infections in preschool children have paid increasing attention to identifying influential factors contributing to frequent episodes of acute respiratory infections (ARI). In particular, these factors include significant deviations in children's physical development from standard values.&#x0D; Aim: to find out the relationship between the frequency of ARI episodes in children aged 60-83 months and their basic anthropometric indicators.&#x0D; Materials and methods. A total of 71 children (44 boys and 27 girls) aged 60-83 months, undergoing inpatient treatment on ARI, were involved in the clinical study.&#x0D; The number of ARI episodes during the previous year of their life was taken into account. Additionally, two integral indices of ARI recurrence, specifically the infection index (InI) and resistance index (RI), were calculated. The basic indicators of physical development were assessed in the children, including: 1) body weight; 2) body length; 3) body surface area; 4) body mass index; 5) chest circumference (CC); 6) integral index of dolichostenomelia. For the comparative analysis of the results obtained, the current standards of physical development indicators for children from the World Health Organization were used. The statistical processing of the digital data was performed with IBM SPSS Statistics 28 licensed software.&#x0D; Results. Rank correlation analysis and cross-tabulation did not demonstrate any interdependence between InI and RI, on the one hand, and the anthropometric indicators considered, on the other hand. Meanwhile, there was a very strong direct correlation between InI and RI (ρ=0.983; p&lt;0.001; 95% CI: 0.972-0.989) in the examined children. Linear regression analysis revealed the dependence of InI and RI on CC size (p&lt;0.001). At that, the degree of the impact of CC size on these clinical indices was almost the same, constituting 7.7% and 7.5%, respectively.&#x0D; Conclusion. Thus, according to the data obtained, the contribution of СС size to the susceptibility of children aged 60-83 months to recurrent respiratory infections among other potential risk factors is insignificant. However, given the widely recognized diversity of mentioned factors, the result deserves due attention. This should be accounted while developing the final integrated model for predicting the frequency of ARI episodes in preschool children.

Exploring University Students’ Online Learning Readiness: A Mixed Methods Study of Forced Online Learning

Background: Despite the advancement achieved in previous research into online learning, few studies have used both quantitative and qualitative data to examine how students’ readiness to learn online is affected by three different external factors, comprising (i) the degrees to which technology is available to students, (ii) the support provided by the institutions of learning, and (iii) the social influence affecting the students engaged in forced online learning in a pandemic situation. Purpose: To fill this research gap, this study explored university students’ forced online learning readiness in relation to technological accessibility, institutional support and social influence during a pandemic, in an attempt to furnish insights into how educators can maximize the benefits of adopting online learning methods. Method: A mixed methods research design was employed in this study. Quantitative data, elicited via self-administered questionnaires completed by 211 participants, was analyzed using the frequencies, means, standard deviations and Pearson correlation analysis involving the Statistical Package for the Social Sciences (SPSS) software version 27. Qualitative data, elicited via 11 open-ended questions posed to 41 students through in-depth interviews, was then studied using a thematic analysis of the participants’ feedback concerning the three constructs in online learning. Results: Our quantitative analysis showed that institutional support had the strongest positive correlation with online learning readiness, and this was followed by technology accessibility and social influence in relation to students’ readiness to learn online. Qualitative findings further indicated that students were largely concerned about Internet accessibility and the setting where their roles were restricted to being mere listeners in online sessions. Apart from being apprehensive about excessive online assignments, students also acknowledged that their online interactions were influenced by their friends and family members, and they would prefer practical work that could inspire them to reflect and engage actively with the course material given during the pandemic. Conclusion: While lecturers can make online classes more interactive and discussion-generative, university administrators need to aptly facilitate their institution’s transition to the forced online learning mode, moderate social influence, improve the learning management system, and provide training to teachers and students on the use of emerging technology.

A Comparison of Conventional Blocks and Stabilized Earth Blocks as Building Materials in Uganda

In Uganda, the high cost of conventional cement and sand blocks has driven the widespread adoption of alternative building materials, such as wattle bricks, compressed earth blocks, and burnt bricks. Among these, compressed earth blocks have gained popularity due to their eco-friendly, energy-efficient, and biodegradable nature. Made by compressing damp soil at high pressure, these blocks offer an environmentally sustainable solution by minimizing deforestation and eliminating the need for firewood, which is often used in the production of traditional bricks. This study employed a case study methodology and utilized deliberate sampling to gather data, which was then analyzed using SPSS v20. The findings revealed that Stabilized Earth Materials (SEMs) have a stronger positive correlation with user satisfaction compared to conventional blocks, with a satisfaction rating of 0.651 for SEMs versus 0.602 for conventional blocks. Additionally, the study showed that SEMs had a notable impact on reducing construction costs, with a 4.8% cost difference in favor of SEMs. Based on these results, it is recommended that construction managers should consider increasing the use of stabilized earth materials. This shift could not only lead to significant cost reductions but also improve the overall quality of construction, making it a more sustainable and viable option for Uganda’s building industry.

Evaluation of Craniometric Parameters and Efficacy of Posterior Surgical Intervention Types in Basilar Invagination Patients

OBJECTIVEBasilar invagination is one of the most frequently observed abnormalities at the craniovertebral junction, in which the odontoid process of C2 prolapses into the foramen magnum. METHODSThe current study included 27 patients who underwent surgery for basilar invagination between October 2013 and January 2023. The study group was divided into 2 groups according to basilar invagination types; type I (the presence of type A atlantoaxial instability and instability is the main pathology) and type II (the presence of type B and C atlantoaxial instability and skull base dysgenesis is the main pathology). Craniometric parameters included in the study were; atlantodental interval, posterior atlantodental interval, Chamberlain’s line violation, clivus-canal angle, Welcher’s basal angle, and Boogaard angle. RESULTSThe mean age of the patients was 24.30 ± 14.36 years (5-57 years). Fourteen patients (51.9%) were female, and 13 patients (48.1%) were male. Ten patients (37%) had type I basilar invagination, 17 patients (63%) had type II basilar invagination. Preoperative and postoperative atlantodental interval and Boogaard angle were significantly higher in type I basilar invagination, as preoperative and postoperative posterior atlantodental interval and clivus-canal angle were significantly higher in type II basilar invagination. There was a positive strong correlation between Chamberlain‘s line violation and Boogaard angle. Postoperative Chamberlain‘s line violation was significantly higher in occipitocervical fixation (p=0.035). C1 lateral mass screw fixation was found more successful in Chamberlain‘s line violation correction than occipital plates. Occipitocervical fixation was found to be associated with higher postoperative Nurick scores (p=0.015) and complication rates (p=0.020). Cages applied to the C1-C2 joint space were found to be associated with higher fusion rates (p = 0.023) and lower complication rates (p = 0.024). CONCLUSIONSIn the present study, it was found that C1-C2 fixation was more successful in correcting craniometric parameters and had lower complication rates than occipitocervical fixation. In appropriate patients, it was determined that cage application increased the success rates of the operations.

Soils of xerophytic meadows of the Middle Podesen’ya: on the issues of accumulation of organic substances and biochemical activity

Relevance. Natural meadow communities, belonging to the dry meadows group, in the conditions of a decrease in floodplain of the leading water artery in the Bryansk region — the Desna River are characterized by significant changes in the mode of economic use. However, a significant part of dry meadows is used as pasture lands, so the regime of intensive exploitation of these lands significantly changes the ecological regimes of phytocenoses, reducing the carbon-depositing functions. For xerophytic (dry) meadows of natural origin, the establishment of soil carbon reserves, the identification of biochemical and microbiological activity is especially important in the light of the low stability of these communities under pasture load and wide distribution throughout the territory of the Middle Podesen’ya.Methods. The research provided for field route studies and in-house data processing using the methods of trial sites, the method of ecological and floral classification, the method of ecological scales (according to G. Ellenberg), ecological and chemical methods — determination of organic carbon, the weight method, biochemical and microbiological methods for the study of the biocompatible body.Results. For the soils of xerophytic (dry) meadows, for the first time, carbon-absorbing capacity, enzymatic activity for urease, catalase, cellulase, depending on the phytocenotic complex, has been established for the Middle Podesen’ya. A positive strong correlation has been established with organic carbon and average monthly precipitation in June, July and August. Over a two-year observation period, the amount of total organic carbon in the soil changed in a number of associations communities: Festuco ovinae-Koelerium delavignei &gt; control &gt; Agrimonio eupatoriae-Poetum angustifoliae &gt; Caro carvi-Deschampsietum &gt; Anthoxantho-Agrostietum tenuis &gt; Hieracio pilosellae-Agrostietum tenuis &gt; Koelerio delavignei-Festucetum rubrae &gt; Anthyllidi-Trifolietum montani &gt; Polygalo vulgaris-Anthoxanthoetum.

Enhancing pakchoi (Brassica chinensis L.) agriculture with magnetized-ionized brackish water and organic fertilizers: A sustainable approach to soil quality and crop yield optimization

The scarcity of freshwater, the increasing use of brackish water (B) for irrigation, and the judicious application of organic fertilizers have significant research and practical value for environmental science, water resource management, and clean production. This study investigated the combined impact of B, magnetized-ionized brackish water (MIB), and organic fertilizer on pakchoi (Brassica chinensis L.) growth, yield, and soil quality. Over a two-year period (2022–2023), this study explored the impacts of two types of irrigation water (B and magnetized-ionized brackish water, MIB) in combination with varying rates of water-soluble bio-organic fertilizer (0, 20, 40, 60, and 80 kg/ha, corresponding to treatments 0 through 4, respectively). The findings indicated that the application of 60 kg/ha of organic fertilizer greatly improved the growth rate of pakchoi in terms of plant height, leaf area index, and fresh weight. Moreover, under MIB irrigation, the fresh weight growth rate of pakchoi was superior to that observed under B irrigation. Notably, B irrigation necessitated additional fertilizer to maintain elevated levels of alkali-hydrolyzed nitrogen (AN) and soil quality. Furthermore, the use of organic fertilizer substantially enhanced soil quality, with AN, available phosphorus (AP), organic matter (OM), and soil quality index (SQI) demonstrating a strong positive correlation (P < 0.05). Mantel test analysis showed significant correlations (P < 0.05) between soil OM, SWC, AP, and pakchoi growth and yield. According to the partial least squares path modeling analysis, the use of organic fertilizer had a relatively minor effect on the yield and quality of pakchoi under B irrigation, with path coefficients of 0.088 and 0.298, respectively. However, under MIB irrigation, organic fertilizer enhances yield and quality by improving soil quality (path coefficients of 0.914). In conclusion, the application of MIB irrigation coupled with organic fertilizer contributed to sustainable soil fertility, enhanced crop productivity, and mitigated the environmental impacts associated with conventional irrigation and fertilization methods. Our research underscores the importance of supporting agricultural research and development focused on sustainable irrigation and fertilization methods for farmers and policymakers. This integrated approach offers a harmonious solution for optimizing crop yields and enhancing water use efficiency, and the reduction of in chemical pollutants within agricultural production systems.

Maize cultivar mixtures increase aboveground biomass and grain quality via trait dissimilarity and plasticity

Despite being visually cryptic, conspecific cultivar mixtures increase diversity and can increase productivity in agricultural systems. However, the mechanisms underlying this increase are unclear. In natural systems, differences in traits among species and among ecotypes correspond with diversity-increased productivity, but whether these attributes are important in cultivar mixtures is still unclear. To explore how cultivar mixtures affect agroecosystem productivity and the roles of trait dissimilarity, we conducted a four-year (2017–2020) field experiment in Northeast China with maize. The experimental treatments included eight single cultivar monocultures, four two-cultivar mixtures, one four-cultivar mixture, one six-cultivar mixture and one eight-cultivar mixture. Experiments were arranged in a randomized complete block design with three replications. Across the four years, all cultivar mixture combinations increased aboveground biomass and crude protein in grain by 7.0% and 4.1%, respectively, compared to the average of the eight monocultures. Grain yield, aboveground biomass, and grain crude protein in overyielding cultivar combinations increased by 2.0–9.0%, 13.0–20.0% and 4.8–10.7%, respectively, compared to the average of the component monocultures of each mixture over four years. We calculated positive complementarity effects for grain yield, aboveground biomass and grain crude protein. All selection effects of cultivar mixtures were negative or neutral. There were strong positive correlations between complementarity effect and functional trait dissimilarity in cultivar mixtures, based on grain yields (P< 0.010), aboveground biomass (P< 0.001) and grain crude protein (P< 0.050), whereas there were no such positive relationships between selection effects and functional trait dissimilarity. Moreover, we found higher levels of trait dissimilarity for shoot diameter, leaf area and specific leaf area in cultivar mixtures than in monocultures. Also, the shoot diameter, leaf area and specific leaf area of specific cultivars were higher in mixtures than in monocultures. Our results indicated that niche complementarity derives from functional trait dissimilarity, and strong complementarity effects correlate with greater functional trait dissimilarity in cultivar mixtures. These findings provide evidence that plasticity in traits, rather than only inherent traits, contributed to the diversity effects, and the greater functional trait dissimilarity among cultivars in mixtures can increase crop productivity via increasing cryptic diversity in agroecosystems. Our results link changes to plant aboveground traits in cultivar mixtures, which shed light on yield advantages in diverse agroecosystems.

Newborn Hearing Screening Results for Infants With Prenatal Opioid Exposure in Southern Appalachia.

Infants prenatally exposed to opioids exhibit withdrawal symptomology that introduce physiological noise and can impact newborn hearing screening results. This study compared the referral rate and physiological noise interpreted by number of trials rejected due to artifact on initial newborn hearing screenings of infants with prenatal opioid exposure (POE) and infants with no opioid exposure (NOE). Furthermore, within the POE group, it examined the relationship of referral rates with severity of withdrawal symptomology, and with maternal and infant risk factors. This study used a retrospective cohort design of electronic medical records from six delivery hospitals in South-Central Appalachia. Newborn hearing screenings were conducted using automated auditory brainstem response (ABR) for 334 infants with POE and 226 infants with NOE. Severity of withdrawal symptomology was measured using the Modified Finnegan Neonatal Abstinence Scoring Tool, which includes observation of behaviors that introduce physiological noise. There was no significant difference in newborn hearing screening referral rate between infants with POE and infants with NOE. Referral rate was not affected by maternal or infant risk factors. Infants with POE had statistically significant higher artifact (defined as rejected ABR sweeps) than infants with NOE. There was a strong positive correlation between Finnegan scores and artifact but not referral rates. Sensitivity and specificity analysis indicated artifact decreased substantially after Day 4 of life. Referral rates of infants with POE were similar to those of infants with NOE. Nevertheless, the withdrawal symptomology of infants with POE introduces physiological noise reflected as artifact on ABR, which can affect efficiency of newborn hearing screenings.

Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes

It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p&lt;0.001). Overall, 53% (116/216) of infected Anopheles stephensi mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34–501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p&lt;0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, Anopheles coluzzii mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171–2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p&lt;0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p&lt;0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential.

Abstract 1696: ACTL6A deficiency drives cell death through KLF4-involved chromatin accessibility changes in colorectal cancer

Abstract ACTL6A is a subunit of the SWI/SNF and INO80 chromatin remodeling complexes and plays roles in neural development and cancer cell proliferation. Additionally, it is known to be upregulated in various types of cancer and is associated with a decrease in patient survival rates. However, the mechanism of how chromatin remodelers including ACTL6A cause cell death has yet to be defined. Therefore, we would like to investigate how the depletion of ACTL6A promotes cell growth reduction by mediating changes in the epigenetic landscape in colorectal cancer cells. We engineered ACTL6A knockout by CRISPR-Cas9. We performed Assay for transposase-accessible chromatin using sequencing (ATAC-seq), RNA sequencing (RNA-seq), and Chromatin Immunoprecipitation sequencing (ChIP-seq) for SMARCB1 and INO80, as representative members of their respective families, and KLF4. Moreover, HCT116 cells stably transduced with doxycycline-inducible shGFP or shACTL6A were implanted into the flank of nude mice. One week after tumor implantation, mice were randomly allocated to 4 groups (n=13-15 per group) and doxycycline treated group was administered with doxycycline (1mg/ml) and 5% sucrose in the drinking water. Tumor volume was measured with calipers. Upon depletion of ACTL6A, the regions of increased chromatin accessibility were correlated with the upregulated genes. After the loss of ACTL6A, there was a significant increase in KLF4 expression and genomic binding largely increased, coinciding with an increase in newly accessible regions containing KLF4 motifs. Interestingly, there is a strong positive correlation between changes in chromatin accessibility and overall changes in the binding of KLF4, SWI/SNF, and INO80 complexes. We find that changes in chromatin accessibility are co-regulated by KLF4, the SWI/SNF and the INO80 complex, while SWI/SNF and INO80 complex have a low correlation in binding between the SWI/SNF and INO80 complexes at newly accessible regions, indicating no physical interactions between these two chromatin complexes. Overall, we demonstrated that the redistribution of ACTL6A-deficient two chromatin remodeling complexes and KLF4 is associated with changes in chromatin accessibility, which are also linked to upregulated genes that promote the p53 pathway. These findings revealed the role of KLF4 along with the SWI/SNF and INO80 complexes, in regulating the chromatin landscape and gene expression in ACTL6A-depleted colorectal cancer cells. Citation Format: Hye-Ju Yang, Eun-Ju Kim, Sang-Hyun Song, Tae-You Kim. ACTL6A deficiency drives cell death through KLF4-involved chromatin accessibility changes in colorectal cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 1696.

Cystatin C Outperforms Creatinine in Predicting Cefepime Clearance in Pediatric Stem Cell Transplant Recipients

BackgroundPediatric hematopoietic stem cell transplant (HSCT) patients are at risk of developing both sepsis and altered kidney function. Cefepime is used for empiric coverage post-HSCT and requires dose adjustment based on kidney function. Since cefepime's anti-microbial efficacy is determined by the time free concentrations exceed bacterial minimum inhibitory concentration (MIC), it is important to assess kidney function accurately to ensure adequate concentrations. Serum creatinine (SCr) is routinely used to estimate glomerular filtration rate (eGFR) but varies with muscle mass, which can be significantly lower in HSCT patients, making SCr an inaccurate kidney function biomarker. Cystatin C (CysC) eGFR is independent of muscle mass, though steroid use increases CysC. ObjectivesTo describe how eGFR impacts cefepime pharmacokinetic/pharmacodynamic (PK/PD) target attainment in pediatric HSCT patients, to investigate which method of estimating GFR (SCr, CysC, combined) best predicts cefepime clearance, and to explore additional predictors of cefepime clearance. Study DesignPatients admitted to the pediatric HSCT unit who received ≥2 cefepime doses were prospectively enrolled. We measured total cefepime peak/trough concentrations between the 2nd-4th cefepime doses and measured SCr and CysC if not already obtained clinically within 24h of cefepime samples. eGFRs were calculated with Chronic Kidney Disease in Children U25 equations. Bayesian estimates of cefepime clearance were determined with a pediatric cefepime PK model and PK software MwPharm++. Simple linear regression was used to compare cefepime clearance normalized to body surface area (BSA) to BSA-normalized SCr-, CysC-, and SCr-/CysC-eGFRs, while multiple linear regression was used to account for additional predictors of cefepime clearance. For target attainment, we assessed the percentage of time free cefepime concentrations exceeded 1x MIC (%fT>1x MIC) and 4x MIC (%fT>4x MIC) using a susceptibility breakpoint of 8 mg/L for Pseudomonas aeruginosa. ResultsWe enrolled 53 patients (ages 1-30 years, median 8.9 years). SCr- and CysC-eGFRs were lower in patients who attained 100% fT>1xMIC compared to those who did not attain this target: 115 vs 156 mL/min/1.73m2 (p=0.01) for SCr-eGFR and 73.5 vs 107 mL/min/1.73m2 (p<0.001) for CysC-eGFR. SCr-eGFR was weakly positively correlated with cefepime clearance (adjusted [a]r2=0.14), while CysC-eGFR and SCr-/CysC-eGFR had stronger positive correlations (ar2=0.30 CysC, ar2=0.28 combo. There was a weak, significant linear association between increasing CysC-eGFR and decreased %fT>1xMIC (ar2=0.32) and %fT>4xMIC (ar2=0.14). No patients with a CysC-eGFR >120 mL/min/1.73 m2 achieved 100% fT>1xMIC or 50% fT>4x MIC. In multiple regression models, underlying diagnosis of hemoglobinopathy (in all models) and being pre-transplant (in SCr and combined models) were associated with increased cefepime clearance, while concomitant use of calcineurin inhibitors was associated with decreased cefepime clearance in all models. Overall, the combo-eGFR model with timing pre-transplant, hemoglobinopathy, and use of calcineurin inhibitors had the best performance (ar2=0.63). ConclusionsCysC-based eGFRs (CysC alone and combined) predicted cefepime clearance better than SCr-eGFR, even after considering steroid use. Increasing CysC eGFR correlated with decreased probability of PD target attainment, raising concerns for underdosing at high eGFRs. CysC should be included when estimating kidney function to provide adequate dosing of cefepime in pediatric HSCT patients.

Investigating the multifaceted dynamics of cybersecurity practices and their impact on the quality of e-government services: evidence from the KSA

PurposeThe Kingdom of Saudi Arabia (KSA) is embracing digital transformation and e-government services, aiming to improve efficiency, accessibility and citizen-centricity. Nonetheless, the country faces challenges such as evolving cyber threats. The purpose of this study is to investigate the factors influencing cybersecurity practices to ensure the reliability and security of e-government services.Design/methodology/approachThis paper investigates the multifaceted dynamics of cybersecurity practices and their impact on the quality and effectiveness of e-government services. Five key factors explored include organizational culture, technology infrastructure, adherence to standards and regulations, employee training and awareness and financial investment in cybersecurity. This study used a quantitative method to gather data from 320 participants. The researcher collected 285 completed questionnaires, excluding unusable or incomplete responses, and analyzed the final data set using partial least squares structural equation modeling.FindingsThe findings show that financial investment in cybersecurity, employee training and awareness and adherence to cybersecurity regulations significantly influence the adoption of robust cybersecurity practices. However, the relationship between organizational culture and cybersecurity practices is less straightforward. The research establishes a strong positive correlation between cybersecurity practices and e-government service quality, highlighting the role of security in fostering public trust and user satisfaction and meeting the evolving needs of citizens and businesses.Originality/valueThis research contributes valuable empirical evidence to the fields of e-government and cybersecurity, offering insights that can inform evidence-based policy decisions and resource allocation. By understanding the nuanced dynamics at play, Saudi Arabia is better poised to fortify its digital governance infrastructure and provide secure, high-quality e-government services to its constituents.

Exploring place attachment: Insights from post-Gorkha earthquake resettlement planning in Nepal

This study investigates the relationship between place attachment and resettlement planning in Nepal post the Gorkha Earthquake of 2015. Focusing on functional place dependence, it analyses place attachment within the Panipokhari Integrated Settlement, designed for the Thami community in Dolakha district. Using "residential satisfaction" as a parameter to measure the influencing factors of place dependence, data were collected through a Likert scale survey, interviews, and observations. The analysis encompassed Cronbach's Alpha for reliability (α = 0.825), mean satisfaction scores for evaluation, and Spearman’s rank correlation to explore relationships between residential satisfaction parameters and place dependence factors. The study revealed significant correlations, highlighting varied influences on place attachment. Notably, social connectivity, represented by nearness to relatives (r = 0.720, p = 0.000), exhibited a strong positive correlation. Similarly, infrastructure amenities like electricity (r = 0.532, p = 0.000) displayed a relatively strong positive correlation, while access to health facilities (r = 0.447, p = 0.002) demonstrated a moderate positive correlation. Moreover, settlement planning components—kitchen gardens (r = 0.623, p = 0.000), open spaces (r = 0.578, p = 0.000), settlement layout (r = 0.549, p = 0.000), and cattle sheds (r = 0.574, p = 0.000)—significantly correlated with increased attachment. Additionally, housing design factors like immediate exterior spaces (r = 0.730, p = 0.000), spaces for life cycle rituals (r = 0.771, p = 0.000), and social events (r = 0.663, p = 0.000) and building performance factor winter thermal comfort (r = 0.606, p = 0.000)displayed substantial positive correlations. Acknowledging study limitations, it offers insights for planners and policymakers, stressing holistic approaches for sustainable resettlement. Future research should explore diverse factors and contexts, emphasizing the dynamic nature of place dependence. Ultimately, the study underscores the intricate relationship between satisfaction parameters and place attachment, urging comprehensive resettlement strategies.

The Lower Limb Muscle Co-Activation Map during Human Locomotion: From Slow Walking to Running.

The central nervous system (CNS) controls movements and regulates joint stiffness with muscle co-activation, but until now, few studies have examined muscle pairs during running. This study aims to investigate differences in lower limb muscle coactivation during gait at different speeds, from walking to running. Nineteen healthy runners walked and ran at speeds ranging from 0.8 km/h to 9.3 km/h. Twelve lower limb muscles' co-activation was calculated using the time-varying multi-muscle co-activation function (TMCf) with global, flexor-extension, and rostro-caudal approaches. Spatiotemporal and kinematic parameters were also measured. We found that TMCf, spatiotemporal, and kinematic parameters were significantly affected by gait speed for all approaches. Significant differences were observed in the main parameters of each co-activation approach and in the spatiotemporal and kinematic parameters at the transition between walking and running. In particular, significant differences were observed in the global co-activation (CIglob, main effect F(1,17) = 641.04, p < 0.001; at the transition p < 0.001), the stride length (main effect F(1,17) = 253.03, p < 0.001; at the transition p < 0.001), the stride frequency (main effect F(1,17) = 714.22, p < 0.001; at the transition p < 0.001) and the Center of Mass displacement in the vertical (CoMy, main effect F(1,17) = 426.2, p < 0.001; at the transition p < 0.001) and medial-lateral (CoMz, main effect F(1,17) = 120.29 p < 0.001; at the transition p < 0.001) directions. Regarding the correlation analysis, the CoMy was positively correlated with a higher CIglob (r = 0.88, p < 0.001) and negatively correlated with Full Width at Half Maximum (FWHMglob, r = -0.83, p < 0.001), whereas the CoMz was positively correlated with the global Center of Activity (CoAglob, r = 0.97, p < 0.001). Positive and negative strong correlations were found between global co-activation parameters and center of mass displacements, as well as some spatiotemporal parameters, regardless of gait speed. Our findings suggest that walking and running have different co-activation patterns and kinematic characteristics, with the whole-limb stiffness exerted more synchronously and stably during running. The co-activation indexes and kinematic parameters could be the result of global co-activation, which is a sensory-control integration process used by the CNS to deal with more demanding and potentially unstable tasks like running.

Assessment of polychaete diversity in selected tropical Islands and estuaries on the Southeast coast of India

Increased anthropogenic pressure on the marine environment leads to severe threats to resident biota, a global concern. The Gulf of Mannar (GoM) and Palk Bay (PB) on the Southeast coast of India are two notable ecological hotspots. However, increased industrialization, tourism, and fishing activities in recent years have contaminated the environment and biota of these two ecologically significant coastal zones. This study aims at the impact of seasonal dynamics on polychaete assemblages in four coastal areas (Kanyakumari, Thoothukudi, Ramanathapuram, and Cuddalore) along the southeast coast with different seasons (post-monsoon, summer, pre-monsoon, and monsoon). In all the collected parameters, salinity is the prime variable influencing the distribution of polychaetes greatly; they link to other environmental parameters. Results revealed that temperature, pH, chlorophyll, and total organic carbon all exhibited a strong positive correlation with salinity; others showed a negative correlation. According to a one-way ANOVA, the Kruskal-Wallis test and Pearson correlation have significant differences at the spatial scale (p<0.05). Overall, the multivariate analysis of PCA and CCA, followed by indices like Shannon diversity index and AMBI and M-AMBI indices results, revealed that spatial heterogeneity of the polychaetes was influenced by salinity, temperature, pH, DO, TN, TP, TOC, and clay (p<0.05). The environmental changes and anthropogenic activities are the reason for the spatial variation in the polychaetes.