Abstract The Yellowstone region (western United States) is a commonly cited example of intraplate volcanism whose origin has been a topic of debate for several decades. Recent work has suggested that a deep mantle plume, rooted beneath southern California, is the source of Yellowstone volcanism. Seismic anisotropy, which typically results from deformation, can be used to identify and characterize mantle flow. Here, we show that the proposed plume root location at the base of the mantle is strongly seismically anisotropic. This finding is complemented by geodynamic modeling results showing upwelling flow and high strains in the lowermost mantle beneath the Yellowstone region. Our results support the idea that the Yellowstone volcanism is caused by a plume rooted in the deepest mantle beneath southern California, connecting dynamics in the deepest mantle with phenomena at Earth's surface.