Strong Scavenging Activity Research Articles

Purification and Structural Characterization of Dendrobium officinale Polysaccharides and Its Activities.

In this study, Dendrobium officinale polysaccharide (named DOPS-1) was isolated from the stems of Dendrobium officinale by hot-water extraction and purified by using Sephadex G-150 column chromatography. The structural characterization, antioxidant and cytotoxic activity were carried out. Based on the results of HPLC, GC, Congo red experiment, together with periodate oxidation, Smith degradation, SEM, FT-IR, and NMR spectral analysis, it expressed that DOPS-1 was largely composed of mannose, glucose and galacturonic acid in a molar ratio of 3.2 : 1.3 : 1. The molecular weight of DOPS-1 was 1530 kDa and the main chain was composed of (1→4)-β-D-Glcp, (1→4)-β-D-Manp and 2-O-acetyl-(1→4)-β-D-Manp. The measurement results of antioxidant activity showed that DOPS-1 had the strong scavenging activities on hydroxyl radicals, DPPH radicals and superoxide radicals and the high reducing ability in vitro. Moreover, DOPS-1 was cytotoxic to all three human cancer cells of MDA-MB-231, A549 and HepG2.

Structurally characterized zinc complexes of flavonoids chrysin and quercetin with antioxidant potential

Chrysin and quercetin are two of the most prominent and bioactive flavonoids with a wide spectrum of beneficial properties, including antioxidant and radical scavenging activity. The complexation of these flavonoids with transition metal ions of biological interest can lead to the generation of novel metallodrugs with improved pharmacological and biochemical properties. Within this framework, the synthesis and detailed structural and physico-chemical characterization of two novel heteroleptic complex assemblies of Zn(II) with chrysin and quercetin and the ancillary aromatic chelator 2,2′-bipyridine is presented. The two complexes represent the only crystallographically characterized structures with Zn(II) as the central metal ion and chrysin or quercetin as the ligands. The new complexes were biologically evaluated in vitro for their antioxidant potential, both exhibiting strong radical scavenging activity in the 2,2-diphenyl-1-picrylhydrazyl assay, and merit further investigation of their pharmacological profile.

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production.

Levan is an industrially important, functional biopolymer with considerable applications in the food and pharmaceutical fields owing to its safety and biocompatibility. Here, levan-type exopolysaccharide produced by Pantoea agglomerans ZMR7 was purified by cold ethanol precipitation and characterized using TLC, FTIR, 1H, and 13C NMR spectroscopy. The maximum production of levan (28.4 g/l) was achieved when sucrose and ammonium chloride were used as carbon and nitrogen sources, respectively, at 35°C and an initial pH of 8.0. Some biomedical applications of levan like antitumor, antiparasitic, and antioxidant activities were investigated in vitro. The results revealed the ability of levan at different concentrations to decrease the viability of rhabdomyosarcoma and breast cancer cells compared with untreated cancer cells. Levan appeared also to have high antiparasitic activity against the promastigote of Leishmania tropica. Furthermore, levan had strong DPPH radical scavenging (antioxidant) activity. These findings suggest that levan produced by P. agglomerans ZMR7 can serve as a natural biopolymer candidate for the pharmaceutical and medical fields.

Characterization, Antimicrobial and Antioxidant Evaluation of Biofabricated Silver Nanoparticles from Endophytic Pantoea anthophila.

Endophyte mediated nanoparticles fabrication were emerging as a new frontier in nanomedicines that produce high biocompatible and functionalized silver nanoparticles. In this study, silver nanoparticles were successfully biosynthesized from the extracellular extract of endophytic bacterium Pantoea anthophila isolated from the stem of Waltheria indica for the first time. The synthesized nanoparticles showed a strong absorption band at 410 nm in the UV–Visible range. The dynamic light scattering and zeta potential analysis indicated that the average particle size was 16 nm at 5.30 mV. FTIR spectrum displayed the presence of various functional groups at 3423.65, 1633.71, 1022.27, 607.58 cm−1 that stabilised the nanoparticle. X-ray diffraction peaks were conferred to 100, 200, 220 and 311 planes of a face centred cubic structure. TEM and SEM micrograph revealed the spherical-shaped, polycrystalline nature with the presence of elemental silver analysed by EDAX. Selected area electron diffraction also confirms the orientation of silver nanoparticles with X-ray diffraction analysis. Antimicrobial activity against 10 different human pathogenic bacteria and fungi showed a broad spectrum inhibition against both Gram-positive and Gram-negative bacteria. Among the bacterial pathogens, B. Subtilis exhibited low activity compared to other pathogens. C. albicans was greatly controlled than other fungal species. A strong free radical scavenging activity of silver nanoparticles with IC50 values 31.29 ± 0.73, 19.83 ± 1.57, 35.64 ± 0.94, 42.07 ± 1.30, 29.70 ± 2.26, 29.10 ± 0.82, 36.80 ± 0.63 μg/ml was obtained in different antioxidant assays that were comparable to the reference. The study suggests that the silver nanoparticles can be biosynthesized from endophytic P. anthophila metabolites with significant therapeutic potential. With proper validation, the biosynthesized silver nanoparticles can be developed as a promising antiviral and anticancer drug candidate.

The structural characteristic of acidic-hydrolyzed corn silk polysaccharides and its protection on the H2O2-injured intestinal epithelial cells

This work aims to describe the purification and characterization of acidic-hydrolyzed corn silk polysaccharides (AH-CSP) and evaluate their protection on the H2O2-injured intestinalepithelial cells (IEC-6). Two fractions named AHP-1 and AHP-2 were obtained from AH-CSP, and physicochemical properties of them were investigated by gel permeation chromatography (GPC), gas chromatography (GC), nuclear magnetic resonance (NMR), fourier transform infrared (FT-IR) spectroscopy, scanning electronic microscopy (SEM), and Congo red test. Results showed that AHP-1 (2.80 × 104 Da) and AHP-2 (1.25 × 104 Da) were consisted of xylose, mannose, galactose, rhamnose, arabinose, and glucose. AHP-1 and AHP-2 had strong scavenging activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-Azobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and OH· free radicals. Furthermore, pretreatment with AHP-2 could protect the H2O2-injured IEC-6 cells by effectively scavenging the overproduced reactive oxygen species (ROS) and regulating of Kelch-like ECH-associated protein 1(Keap1)/ nuclear factor erythroid 2-related factor 2 (Nrf2) signal pathway.

Extraction, purification, physicochemical properties and antioxidant activity of a new polysaccharide from Ocimum album L. seed

In this study, a novel polysaccharide fraction from Ocimum album seed was extracted and then purified by Cellulose DEAE-52 and Sephadex G-200 anion exchange chromatography. The structural, physicochemical and antioxidant properties of the main polysaccharide fraction (OAP-1A) were evaluated. The purified polysaccharide contained 94.3% carbohydrate, 3.56% moisture and 2.14% ash and result of gel permeation chromatography (GPC) showed average molecular weight of 593 kDa. The results of high-performance liquid chromatography (HPLC) showed that OAP-1A was a neutral hetero-polysaccharide composed of mannose (35.7%), glucose (33.32%), galactose (19.6%) and rhamnose (11.38%). In addition, GC–MS data, nuclear magnetic resonance (NMR) spectrum and Fourier transform infrared (FT-IR) analysis revealed that the backbone of OAP-1A consists of →3)-β-D-Manp-(1→, →3,4)-β-D-Manp-(1→, →3,6)-β-D-Manp-(1→, →3)-α-D-Glcp-(1→, →6)-β-D-Galp-(1→, →4)-α-L-Rhap-(1→ and α-D-Glcp-(1→. X-ray diffraction (XRD) analysis showed semi-crystalline structure in OAP-1A. Differential scanning colorimeter (DSC) and thermo-gravimetry analysis (TGA) indicated that OAP-1A had relatively high thermal stability. Moreover, OAP-1A showed strong scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals.

Synthesis and antioxidant activity of zinc(II) phthalocyanine tetranitroxide

Zinc(II) phthalocyanine (np-TEMPO-ZnPc) bearing four TEMPO fragments at the non-peripheral positions of the macrocycle was prepared through the cyclotetramerization of phthalonitrile derivative 3-TEMPO-Pht. Characterization of tetraradical was performed by electron paramagnetic resonance (EPR) at room temperature. A characteristic triplet was observed (g = 2.00715, hyperfine coupling constant of 15.73 G) in the EPR spectrum of np-TEMPO-ZnPc measured in chloroform. The np-TEMPO-ZnPc was also investigated for in vitro antioxidant activities. Antioxidant properties were determined for its free radical scavenging effect, reducing power, β-carotene bleaching and total antioxidant capacity assays. The results demonstrated that np-TEMPO-ZnPc has strong DPPH radical scavenging and β-carotene bleaching activities even more than the standard antioxidant BHT. Especially, the compound exhibited similar radical scavenging effect at low concentrations when compared with BHT.

Potential Antioxidant Properties of Enzymatic Hydrolysates from Stichopus japonicus against Hydrogen Peroxide-Induced Oxidative Stress.

A comprehensive antioxidant evaluation was performed on enzymatic hydrolysates of Stichopus japonicus (S. japonicus) using Vero cells and zebrafish models for in vitro and in vivo studies, respectively. S. japonicus was hydrolyzed with food-grade enzymes (alcalase, α-chymotrypsin, flavourzyme, kojizyme, neutrase, papain, pepsin, protamex, and trypsin), and the free radical scavenging activities were screened via electron spin resonance (ESR) spectroscopy. According to the results, the enzymatic hydrolysates contained high protein and relatively low polysaccharide and sulfate contents. Among these hydrolysates, the α-chymotrypsin assisted hydrolysate from S. japonicus (α-chy) showed high yield and protein content, and strong hydroxyl radical scavenging activity. Therefore, α-chy was chosen for further purification. The α-chy was fractionated by ultrafiltration into three ultrafiltration (UF) fractions based on their molecular weight: >10 kDa (α-chy-I), 5–10 kDa (α-chy-II), and <5 kDa (α-chy-III), and we evaluated their antioxidant properties in H2O2 exposed Vero cells. The α-chy and its UF fractions significantly decreased the intracellular reactive oxygen species (ROS) generation and increased cell viability in H2O2 exposed Vero cells. Among them, α-chy-III effectively declined the intracellular ROS levels and increased cell viability and exhibited protection against H2O2 induced apoptotic damage. Furthermore, α-chy-III remarkably attenuated the cell death, intracellular ROS and lipid peroxidation in H2O2 exposed zebrafish embryos. Altogether, our findings demonstrated that α-chy and its α-chy-III from S. japonicus possess strong antioxidant activities that could be utilized as a bioactive ingredient for functional food industries.

Antioxidant and Antibacterial Activity of Nepeta × faassenii Bergmans ex Stearn Essential Oil

This study was designed to evaluate, for the first time, the antioxidant and antimicrobial activities of Nepeta × faassenii essential oil (NEO). Twenty-six compounds were identified by gas chromatography and mass spectrometry analysis, of which 4a alpha,7alpha,7a alpha-nepetalactone (34.12%), elemol (23.23%), spiro(5,6)dodecane (13.73%), and 3,4 alpha-dihydro-4a alpha, 7 alpha, 7a alpha-nepetalactone (7.93%) were the major compounds. The NEO exhibited broad-spectrum antibacterial effects, and possesses potent antifungal activity on Candida albicans and Candida parapsilosis. NEO’s antioxidant activity was evaluated against cold-pressed sunflowers oil by peroxide, thiobarbituric acid, 1,1-diphenyl- 2-picrylhydrazyl radical, and β-carotene/linoleic acid bleaching methods. The NEO showed strong scavenging (IC50: 0.032 ± 0.005 mg/mL) and relative antioxidative activity (RAA%: 92.31 ± 0.17%) in 1,1-diphenyl- 2-picrylhydrazyl radical and β-carotene/linoleic acid bleaching assays, respectively. Moreover, during 24 days of the incubation period, the oil inhibits the primary lipid oxidation significantly better (p &lt; 0.05) than butylated hydroxyanisole (BHA). In the case of secondary lipid oxidation, the oil performed significantly better (p &lt; 0.001) than BHA from day 8 to day 12 of the incubation period. The biological activities recorded suggest that NEO may represent an antioxidant and antimicrobial agent with applications in medicine or the food industry.

Chemical composition, antimicrobial, and antioxidant activities of the essential oils from stem, leaves, and seeds of Caryopteris foetida (D. don) Thell.

Caryopteris foetida (D. Don) Thell. also known as stinking blue beard is an aromatic shrub of family verbenaceae. The essential oils from the leaf, stem, and seeds of C. foetida were extracted and analyzed in search of a novel compound. Over 73 constituents were identified from the essential oils of leaf, seeds, and stem. The major compound identified in the leaf oil was δ-cadinene (15.4%) followed by β-caryophyllene (7.8%), (E)-β-farnesene (8.3%), γ-cadinene (7.5%), spathulenol (7.2%), and τ-muurolol (5.1%). The stem oil predominantly consisted of methyl 7-methylcyclopenta[c]pyran-4-carboxylate (15.8%) along with a significant amount of δ-cadinene (11.6%) and γ-cadinene (5.6%), whereas seed oil was characterized by the presence of β-caryophyllene (14.3%) along with α-humulene (8.6%) and β-bisabolene (9.4%). The essential oils were screened for their in vitro antioxidant potential in terms of radical scavenging, metal chelating and reducing assay. The leaf oil exhibited strong DPPH radical scavenging (IC 50 =5.1±0.2 µg/mL) and reducing activity (IC 50 =3.6±0.5 µg/mL). The antibacterial potential was tested against Salmonela typhimurium, Escherichia coli, Staphylococcus Aureus , and Bacillus megaterium . Maximum activity was exhibited by the essential oils from the leaf and seed. The essential oils were also analyzed for their in vitro anti-inflammatory activity by the protein denaturation method. Both the leaf and stem essential oils exhibited good anti-inflammatory activity with IC 50 value of 12.8±0.0 µg/mL and 17.3±0.0 µg/mL respectively.

Radish root extract targets redox imbalance, inflammatory cytokines, coagulation activity and myocardial degeneration as a potential remedy for doxorubicin- induced cardiotoxicity in rats

Cardiotoxicity is a major limiting factor for clinical use of doxorubicin (DOX) in chemotherapy. This study aimed to evaluateeffectiveness of radish root extract (30mg/kg BW) to attenuate doxorubicin (DOX) - induced cardiotoxicity and to explore involved mechanisms. Phytochemical analysis of tested plant and assessment of antioxidant capacity against DPPH free radical were also performed. Treatment of male rats with DOX (2.5mg/kg BW, i.p.) twice weekly for 4 weeks elicitedmyocardial toxicity characterized by increased serum cTn-I, CK-MB, LDH and AST, decreased heart weight/body weight ratio, cadiomyocytes degeneration and disorganization. These effects were associated with significant reduction in cardiac antioxidants (GSH, SOD,CAT) and total antioxidant capacity, paralleled by significant elevation in levels of xanthine oxidase, malondialdehyde, protein carbonyl, and nitric oxide, indicating augmented oxidative stress. Further elevation in serum inflammatory markers (TNF-d, CRP), adhesion molecules (VCAM-1, ICAM-1), fibrinogen and platelet activating factor, accompanied by marked hematologic disorders were also noticed. Results of radish analysis revealed strong scavenging activity against DPPH and presence of polyphenols, flavonoids, tannins and alkaloids as a major phytoconstituents. Supplementation of radish extract has shown to be effective against DOX-induced cardiotoxicity via enhancing antioxidant capacity and combating oxidative stress, inflammation, coagulation and myocardial histological injury. Therefore, radish root extract can be suggested as a potential cardioprotective remedy for patients on DOX- based chemotherapy which might be attributed to its active constituents and radical scavenging properties.

Extraction, purification, and antioxidant activity of exopolysaccharides produced by Lactobacillus kimchi SR8 from sour meat in vitro and in vivo

ABSTRACT The aim of this study was to evaluate the antioxidant activity of exopolysaccharides (EPS) from Lactobacillus kimchi SR8. The EPS exhibited strong scavenging activities for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals. Two EPS fractions (EPS-1 and EPS-3) exhibited higher antioxidant activities than EPS-2. Low-dose CEPS could significantly increase the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) in the serum and the activities of CAT, GSH-Px and superoxide dismutase (SOD) and total antioxidant activity in the liver. Medium- and high-dose CEPS could significantly increase the activities of CAT and GSH-Px and total antioxidant activity in the serum and the activities of CAT, GSH-Px, and SOD and total antioxidant activity in the liver. High-dose CEPS significantly increased the liver index of mice and the activity of SOD in the serum. CEPS could inhibit the formation of malondialdehyde (MDA) in the serum and liver. This suggests that EPS from Lactobacillus kimchi SR8 possesses potential antioxidant activities.

Ameliorative Effects of Ethanolic Leaf Extract of Physalis angulata (Ewe Koropo) on Diabetic-Induced Wistar Rats in South West Nigeria

Excessive consumption of foods high in calories, lack of exercise and oxidative stress play crucial roles in diabetic physiopathology, and if not diagnosed and treated early, damages to kidney, eyes, heart and nerves are inevitable. Medicinal plants have long been utilized in traditional medicine for the treatment of diseases. In this study, qualitative and quantitative phytochemicals analyses of ethanolic extract of Physalis angulata were carried out using standard biochemical methods. In addition, evaluation of the antioxidant activity and inhibitory potential of the plant extract against key enzymes associated with hyperglycemia-a symptom that characterizes diabetes were done. Antioxidant activities were determined using DPPH (2, 2-diphenyl-1-picryl-hydrazyl-hydrate), FRAP (Ferric Reducing Antioxidant Power), ABTS (2, 2l-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), lipid peroxidation assays while the alpha–glucosidase and α-amylase inhibitory activities were also evaluated. The ethanolic extract of Physalis angulata exhibited significant strong scavenging activity on DPPH, lipid peroxidation, reducing power, ABTS activity as compared with the standard drug Ascorbic Acid against the diabetic group (p<0.05). Furthermore, the ethanolic extract did exhibit significant α-amylase and α–glucosidase activities with IC50 (23.88, 15.10), when compared with standard drug acarbose with IC50 (24.75, 41.74). The findings of this study indicate that the ethanolic extract of Physalis angulata leaves possess anti hyperglycemic properties; and thus provide pharmacological benefits to the ethnomedical use of this plant in the treatment, management and control of type 2 diabetes mellitus.

Phenolic compounds and antioxidant activity of different parts of three mandarin varieties extracts: A comparative study

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel source of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels reveled a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

Composés phénoliques et activité antioxydante des extraits de différentes parties de trois variétés de mandarines: Etude comparative

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel sources of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with a value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels revealed a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, the Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

Protective Effects of Traditional Polyherbs on Cisplatin-Induced Acute Kidney Injury Cell Model by Inhibiting Oxidative Stress and MAPK Signaling Pathway.

Acute kidney injury (AKI) is a disease caused by sudden renal dysfunction, which is an important risk factor for chronic renal failure. However, there is no effective treatment for renal impairment. Although some traditional polyherbs are commercially available for renal diseases, their effectiveness has not been reported. Therefore, we examined the nephroprotective effects of polyherbs and their relevant mechanisms in a cisplatin-induced cell injury model. Rat NRK-52E and human HK-2 subjected to cisplatin-induced AKI were treated with four polyherbs, Injinhotang (IJ), Ucha-Shinki-Hwan (US), Yukmijihwang-tang (YJ), and UrofenTM (Uro) similar with Yondansagan-tang, for three days. All polyherbs showed strong free radical scavenging activities, and the treatments prevented cisplatin-induced cell death in both models, especially at 1.2 mg/mL. The protective effects involved antioxidant effects by reducing reactive oxygen species and increasing the activities of superoxide dismutase and catalase. The polyherbs also reduced the number of annexin V-positive apoptotic cells and the expression of cleaved caspase-3, along with inhibited expression of mitogen-activated protein kinase-related proteins. These findings provide evidence for promoting the development of herbal formulas as an alternative therapy for treating AKI.

EFFECT OF STERCULIA QUADRIFIDA R. BR BARK TO IN VITRO DPPH RADICAL AND GLUTATHIONE PEROXIDASE ON DIABETIC WISTAR RATS

Objective: The aims of the research were to evaluate the antioxidant effect of Sterculia quadrifida R.Br bark by in vitro 1,1-biphenyl-2-picryl-hydrazyl (DPPH) radical scavenging method and glutathione peroxidase (GPx) activity on diabetic Wistar rats model.&#x0D; Methods: S. quadrifida bark powder was extracted in ethanol 70% by maceration method. The antioxidant activity of ethanol extract was tested by DPPH radical scavenging method. The activity of GPx was evaluated from the liver of alloxan-induced diabetic rats. The antioxidant activity was determined by measuring the absorbance using ultraviolet-visible spectrophotometry method.&#x0D; Results: The ethanol extract of S. quadrifida bark had a strong DPPH radical scavenging activity, with the IC50 value was 4.86±0.01 ug/ml, besides that the extract also showed significantly activity of GPx (p&lt;0.05) at a dose of 65, 130, and 260 mg/kg bw.&#x0D; Conclusion: The ethanol extract of S. quadrifida bark had a potent antioxidant activity by DPPH radical scavenging and GPx activity.

Rapid screening and isolation of antioxidants from Eupatorium lindleyanum DC. using CCC target-guided by on-line HPLC-DPPH assay

Counter-current chromatography (CCC) target-guided by on-line HPLC with post-column DPPH assay was established for efficient screening and isolation of large amount of antioxidants from Eupatorium lindleyanum DC. On-line HPLC with post-column DPPH reaction was used to screen the antioxidants and optimize the biphasic solvent system of CCC, then the targeted peaks were purified using CCC. In the present study, three compounds, nepetin, cirsiliol and jaceosidin, were targeted and successively separated from n-butanol fraction of E. lindleyanum DC. by this strategy. All three compounds showed strong DPPH radical scavenging activity. These results confirmed that the strategy would be an efficient and effective method to isolate antioxidants from complex mixtures.

Carotenoid feed supplementation in Argentine penaeoid shrimp broodstock

This work aimed to improve reproductive maturation in two penaeoid species (Artemesia longinaris and Pleoticus muelleri) by using carotenes added in artificial feeds and comparing antioxidant activity and carotenoids profiles. Immature females were kept for 45 days at salinity 33, temperature at 18°C, pH 7, 12:12 h photoperiod, ammonium &lt;0.2mg L-1 and seawater exchanged at 100% per day. Feeding regimes consisted of 50% fresh frozen ingredients (clam muscle and squid mantle) and 50% dry pellets. For each species, three pelletized feeds were tested, one as a control (C) and two supplemented each one with 300 mg astaxanthin (A) and β-carotene (B) kg-1 of diet. At the end of the trials, ovaries, midgut gland, and integument were sampled. In both species, the addition of carotenoids improved ovarian development: 100% of females fed A or B diets were mature, but of the females fed with diet C only 75% (P. muelleri) and 64% (A. longinaris) matured. Ovaries of mature females exhibited the highest concentration of carotenoids. The abundance of free astaxanthin regarding total carotenes, suggests that excess of free astaxanthin could be biotransformed to esterified astaxanthin. The scavenging properties were evaluated against the stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical by electron resonance spectroscopy (EPR). For all feed treatments, the extracts exhibited strong DPPH scavenging activity, without significant differences among them.

Pomegranate Juice Extract Decreases Cisplatin Toxicity on Peripheral Blood Mononuclear Cells.

Background: Lung cancer is one of the most prevalent cancers worldwide. Chemotherapy regimens, targeted against lung cancer, are considered an effective treatment; albeit with multiple fatal side effects. An alternative strategy, nowadays, is using natural products. Medicinal plants have been used, in combination with chemotherapy, to ameliorate side effects. This study aims to investigate the antitumor effect of pomegranate juice (Punica granatum) on human lung adenocarcinoma basal epithelial cells (A549), to check the effect, when combined with low dose cisplatin (CDDP), at different doses. We also have evaluated the potential protective effect of pomegranate on normal peripheral blood mononuclear cells (PBMC). Methods: Phytochemical screening of the extract was done using standard classical tests. Total phenolic and sugar contents were determined using the Folin–Ciocalteu and anthrone reagents, respectively. The antioxidant activity of pomegranate was estimated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The viability of A549 cells and PBMC was evaluated using the neutral red assay. Results: Our results demonstrated that Punica granatum or pomegranate juice (with different concentrations: 150, 300, 600 µg/mL) contained high levels of flavonoids, alkaloids, tanins, lignins, terpenoids, and phenols. The DPPH method showed that pomegranate juice had a strong antioxidant scavenging activity. Neutral red showed that combining pomegranate juice with low dose CDDP (8 µg/mL) decreased the cell viability of A549 cells, by 64%, compared to treatment with CDDP or pomegranate alone. When added to low dose CDDP, pomegranate increased the viability of normal PBMC cells by 46%. Conclusions: These results demonstrated that pomegranate could potentiate the anticancer effect of low dose CDDP on human lung adenocarcinoma cells (A549 cells) and could as well decrease its toxicity on PBMC.