Strong Conservation Research Articles

A High-Throughput Screen for Antiproliferative Peptides in Mammalian Cells Identifies Key Transcription Factor Families.

Transcription factors (TFs) are a promising therapeutic target for a multitude of diseases. TFs perform their cellular roles by participating in multiple specific protein-protein interactions. For example, homo- or heterodimerization of some TFs controls DNA binding, while interactions between TFs and components of basal transcriptional machinery or chromatin modifiers can also be critical. While, in theory, small molecules could be used to disrupt specific protein-protein interfaces required for TF function, in practice, it is difficult to identify small molecules with the necessary specificity and efficacy, likely due to the extensive protein-protein interfaces that often underlie TF function. However, in contrast to small molecules, peptides have the potential to provide both the specificity and efficacy required to disrupt such interfaces. Here, we identified ∼15 peptides that inhibit the proliferation of leukemia cells using a high-throughput pooled screen of a library of 80-mer protein regions (peptides) derived from human nuclear-localized proteins. The antiproliferative peptides were enriched for regions known to be involved in specific TF dimerization, including the basic leucine zipper (bZIP) domain family. One of these bZIP domains, JDP2;bZIP_1, from the TF JDP2, was the top antiproliferative peptide, reducing the proliferation of K562 cells by 2-fold. JDP2;bZIP_1 inhibited AP-1 transcriptional activity and phenocopied JDP2 overexpression, suggesting that the peptide affected proliferation through a native JDP2 mechanism. Unexpectedly, given the strong conservation of the bZIP domain, residues outside of the annotated dimerization domain were critical for the peptide's antiproliferative potency. The peptide-mediated antiproliferative effect initiated erythrocyte differentiation in K562 cells and increased G0/G1 cells across multiple cell line models. We also found that many of the antiproliferative peptides identified in this study, including JDP2;bZIP_1, did not require a nuclear localization signal to function, a potential benefit for delivering these peptides in therapeutic applications.

Effects of hemodynamic load on cardiac remodeling in fish and mammals: the value of comparative models.

The ability of the vertebrate heart to remodel enables the cardiac phenotype to be responsive to changes in physiological conditions and aerobic demand. Examples include exercise-induced cardiac hypertrophy, and the significant remodeling of the trout heart during thermal acclimation. Such changes are thought to occur in response to a change in hemodynamic load (i.e. the forces that the heart must work against to circulate blood). Variations in hemodynamic load are caused by either a volume overload (high volume of blood returning to the heart, impairing contraction) or a pressure overload (elevated afterload pressure that the heart must contract against). The changes observed in the heart during remodeling are regulated by multiple cellular signaling pathways. The cardiac response to these regulatory mechanisms occurs across levels of biological organization, affecting cardiac morphology, tissue composition and contractile function. Importantly, prolonged exposure to pressure overload can cause a physiological response - that improves function - to transition to a pathological response that causes loss of function. This Review explores the role of changes in hemodynamic load in regulating the remodeling response, and considers the cellular signals responsible for regulating remodeling, incorporating knowledge gained from studying biomedical models and comparative animal models. We specifically focus on the renin-angiotensin system, and the role of nitric oxide, oxygen free radicals and transforming growth factor beta. Through this approach, we highlight the strong conservation of the regulatory pathways of cardiac remodeling, and the specific conditions within endotherms that may be conducive to the development of pathological phenotypes.

Evolution of the Cdk4/6-Cdkn2 system in invertebrates.

The cell cycle is driven by cyclin-dependent kinases (Cdks). The decision whether the cell cycle proceeds is made during G1 phase, when Cdk4/6 functions. Cyclin-dependent kinase inhibitor 2 (Cdkn2) is a specific inhibitor of Cdk4/6, and their interaction depends on D84 in Cdkn2 and R24/31 in Cdk4/6. This knowledge is based mainly on studies in mammalian cells. Here, we comprehensively analyzed Cdk4/6 and Cdkn2 in invertebrates and found that Cdk4/6 was present in most of the investigated phyla, but the distribution of Cdkn2 was rather uneven among and within the phyla. The positive charge of R24/R31 in Cdk4/6 was conserved in all analyzed species in phyla with Cdkn2. The presence of Cdkn2 and the conservation of the positive charge were statistically correlated. We also found that Cdkn2 has been tightly linked to Fas associated factor 1 (Faf1) during evolution. We discuss potential interactions between Cdkn2 and Cdk4/6 in evolution and the possible cause of the strong conservation of the microsynteny.

Bioinformatics Analysis and Spatiotemporal Distribution of the fliC Gene and Its Protein Isolated from Escherichia coli-Infected Patients in Eastern Algeria.

The fliC locus in Escherichia coli primarily encodes flagellar (H) antigens. Exploring fliC sequence diversity will shed light on the mechanisms of bacterial pathogenicity. This study examined the presence of fliC mutant strains of E. coli in infected patients from different age groups, sexes and sample types in eastern Algerian provinces over a span of 2 years. This retrospective, cross-sectional study involved three provinces in eastern Algeria: i) Bordj Bou Arreridj, ii) Setif and iii) Batna. A total of 75 E. coli isolates were obtained from the University State Hospital Centre. Two types of analyses were conducted: i) a bioinformatics analysis of the protein sequences translated from the fliC genes, specifically the fliC flagellar sequences and ii) a multifactorial statistical analysis (multiple correspondence analysis [MCA]) of the population of infected patients, considering various parameters. The fliC protein sequences were aligned using the Multiple Alignment using Fast Fourier Transform (MAFFT) programme. The alignment results were then visualised using the MView programme. Finally, a phylogenetic tree was constructed using the maximum likelihood algorithm in MEGA 11 software. Bioinformatics analysis highlighted the strong conservation of the structures of the fliC protein sequences, especially at the two N- and C-terminal ends, and strong variability in the central zone. This remarkable fliC intersequence similarity is corroborated by the presence of protein motifs identified in the PROSITE protein motif database. fliC mutations in E. coli were not detected in the clinical samples of patients from hospitals in the three Algerian Provinces. Our analysis revealed that all the samples exhibited characteristics of wild-type virulent bacteria without mutations. A multicentre study is warranted for epidemiological surveillance of fliC mutant strains for future preventive measures.

Malate dehydrogenase: a story of diverse evolutionary radiation.

Malate dehydrogenase (MDH) is a ubiquitous enzyme involved in cellular respiration across all domains of life. MDH's ubiquity allows it to act as an excellent model for considering the history of life and how the rise of aerobic respiration and eukaryogenesis influenced this evolutionary process. Here, we present the diversity of the MDH family of enzymes across bacteria, archaea, and eukarya, the relationship between MDH and lactate dehydrogenase (LDH) in the formation of a protein superfamily, and the connections between MDH and endosymbiosis in the formation of mitochondria and chloroplasts. The development of novel and powerful DNA sequencing techniques has challenged some of the conventional wisdom underlying MDH evolution and suggests a history dominated by gene duplication, horizontal gene transfer, and cryptic endosymbiosis events and adaptation to a diverse range of environments across all domains of life over evolutionary time. The data also suggest a superfamily of proteins that do not share high levels of sequential similarity but yet retain strong conservation of core function via key amino acid residues and secondary structural components. As DNA sequencing and 'big data' analysis techniques continue to improve in the life sciences, it is likely that the story of MDH will continue to refine as more examples of superfamily diversity are recovered from nature and analyzed.

Evaluating the conservation benefit of circle hooks for the Atlantic Striped Bass recreational fishery

AbstractObjectiveThe Striped Bass Morone saxatilis is one of the most sought‐after recreational fish species along the US Atlantic coast. Regulations and a strong conservation ethic among anglers make Striped Bass among the most frequently released coastal marine fishes. A recent rule requires anglers to use circle hooks when targeting Striped Bass with natural baits, yet the conservation benefit of this action remains unclear.MethodsWe used acoustic telemetry to monitor the fate of Striped Bass that were recreationally caught with baited hooks (n = 349) and to estimate the influence of various biological (fish size), fishery (fight time, handling time, hook location, bait type, and hook type), and environmental (water temperature and air temperature) variables on postrelease survival.ResultA semi‐quantitative score of fish release condition was the single best predictor of mortality. A broader dataset that included untagged fish (n = 716) was used to identify the best predictors of fish release condition, which included hook location and handling time.ConclusionContrary to expectations, the circle hooks used in this study did not result in lower release mortality than conventional J‐hooks.

Whether the summer fishing moratorium can improve the status of fisheries resources in the yellow sea and Bohai Sea

The China government has introduced many strategies in fisheries management of the Yellow Sea and the Bohai Sea, the most stringent of which is the summer fishing moratorium. Whether the summer fishing moratorium can restore fishery resources has always been a concern. In this study, we selected eight representative commercial fish species the Yellow Sea and Bohai Sea, including Pampus argenteus, Trichiurus lepturus, Scomberomorus niphonius, Ilisha elongata, Thamnaconus modestus, Scomber japonicus, Engraulis japonicus and Larimichthys polyactis, and used the JABBA method to study their historical exploited dynamics and current stock status. We evaluated the effectiveness of the summer fishing moratorium based on the increments of biomass and catch after its implementation and adjustments. P. argenteus and S. niphonius stocks were in the healthy status in 2022, the other six fisheries stocks were overexploited. The summer fishing moratorium had a strong conservation effect, especially after the first implementation in 1995, with the biomass of six fish species increased and the catch of seven species increased, and the total average biomass and catch increments were 21.22 % and 89.72 %. This positive effect was also reflected after the first adjustment in 2003. Although the moratorium duration was continuously extended after the second and third adjustment, the conservation effect was offset by the flooding of fishing efforts immediately after the moratorium. For the six overexploited stocks, we suggest that the allowable catch of E. japonicus, T. lepturus, L. polyactis, S. japonicus, I. elongata and T. modestus is at most 527.91 kt, 147.51 kt, 93.43 kt, 81.51 kt, 1.25 kt and 4.95 kt in the Yellow Sea and Bohai Sea, respectively.

Peering through the hedge: Multiple datasets yield insights into the phylogenetic relationships and incongruences in the tribe Lilieae (Liliaceae)

The increasing use of genome-scale data has significantly facilitated phylogenetic analyses, contributing to the dissection of the underlying evolutionary mechanisms that shape phylogenetic incongruences, such as incomplete lineage sorting (ILS) and hybridization. Lilieae, a prominent member of the Liliaceae family, comprises four genera and approximately 260 species, representing 43% of all species within Liliaceae. They possess high ornamental, medicinal and edible values. Yet, no study has explored the validity of various genome-scale data in phylogenetic analyses within this tribe, nor have potential evolutionary mechanisms underlying its phylogenetic incongruences been investigated. Here, transcriptome, Angiosperms353, plastid and mitochondrial data, were collected from 50 to 93 samples of Lilieae, covering all four recognized genera. Multiple datasets were created and used for phylogenetic analyses based on concatenated and coalescent-based methods. Evolutionary rates of different datasets were calculated, and divergence times were estimated. Various approaches, including coalescence simulation, Quartet Sampling (QS), calculation of concordance factors (gCF and sCF), as well as MSCquartets and reticulate network inference, were carried out to infer the phylogenetic discordances and analyze their underlying mechanisms using a reduced 33-taxon dataset. Despite extensive phylogenetic discordances among gene trees, robust phylogenies were inferred from nuclear and plastid data compared to mitochondrial data, with lower synonymous substitution detected in mitochondrial genes than in nuclear and plastid genes. Significant ILS was detected across the phylogeny of Lilieae, with clear evidence of reticulate evolution identified. Divergence time estimation indicated that most of lineages in Lilieae diverged during a narrow time frame (ranging from 5.0 Ma to 10.0 Ma), consistent with the notion of rapid radiation evolution. Our results suggest that integrating transcriptomic and plastid data can serve as cost-effective and efficient tools for phylogenetic inference and evolutionary analysis within Lilieae, and Angiosperms353 data is also a favorable choice. Mitochondrial data are more suitable for phylogenetic analyses at higher taxonomic levels due to their stronger conservation and lower synonymous substitution rates. Significant phylogenetic incongruences detected in Lilieae were caused by both incomplete lineage sorting (ILS) and reticulate evolution, with hybridization and “ghost introgression” likely prevalent in the evolution of Lilieae species. Our findings provide new insights into the phylogeny of Lilieae, enhancing our understanding of the evolution of species in this tribe.

Digital bead modeling for wire-arc directed energy deposition

Prediction of 2D cross-section and full 3D geometry for stacked weld beads is critical for the outcome of wire-arc directed energy deposition (DED) parts; however, most additive path planning software packages model beads as extrusions of a rectangle. Weld beads are not rectangular, and the resulting shape is dependent upon physics effects at the moment of deposition. Physics phenomena such as the geometry of the underlying surface, the heat input of the welding mode, and the direction of gravity contribute to bead shape. This paper presents a novel implicit modeling method that discretizes a 2D area or 3D volume of space into pixels or voxels and constructs fields based on these physics phenomena. The fields are combined using a weighting scheme trained on 3D scan measurements of welds and wire-arc DED prints. Pixels or voxels are added until the known amount of deposited volume has been achieved. Thereby, a strong conservation of mass principle is applied to the process. Utilizing machine learning techniques, the present model can be trained on a database of scans allowing for the representation of a wide variety of prints. Results show that this method can produce predictions with realistic bead morphology and sub-millimeter form error.

Taking stock: assessing Rhode Island recreational angler conservation ethic and coastal identity

ABSTRACT Fisheries management in the United States requires managing the actions of both commercial and recreational fishers in order to maintain its efficacy. To maintain sustainable fisheries, recreational fisheries management relies on individuals’ ethical behaviors and assumptions of their compliance with regulations. This mixed methods study assessed Rhode Island recreational anglers’ conservation ethic in relation to their coastal identities. The results show a positive correlation between ethic and coastal identity among anglers, and evidence that family members and other mentors play a significant role in the development of both coastal identity and angling ethic. We conclude with recommendations of communication strategies that fisheries managers can utilize to highlight anglers’ personal identification with coastal areas in order to encourage strong conservation ethic, and thus sustainable angling behaviors.

Weaving different forms of knowledge of managed aquifer recharge in a Saharan oasis (Algeria)

This paper demonstrates the reciprocal benefits of a socio-hydrology approach that fosters fruitful exchanges between different ‘knowers’ of complex water dynamics in weaving different types of knowledge. For centuries, the managed aquifer recharge and use system in the Beni Isguen oasis in Algeria was diligently monitored by communal water stewards and the data meticulously recorded and stored. Throughout our research project on the origin of groundwater and its replenishment, intense dialogue between scientists and communal water stewards greatly enhanced the research protocol based on isotope tracers, while simultaneously contributing to the active knowledge base of the community. The dialogue was based on mutual respect, trust and a reciprocal desire to share knowledge. Our findings revealed increased mineralization of shallow groundwater during drought periods, which was attributed to geological processes, and emphasized the crucial role of floods. Also, contrary to the initial assumption that deep Continental Intercalaire groundwater was only used for drinking, it was identified as a vital external resource, responsible for substantial recharge of the phreatic aquifer of the oasis. This source of water explains the continued use of the phreatic aquifer for irrigation more than 10 years after the last major flood. This collaborative socio-hydrology approach between differently situated ‘knowers’ contributes to the grounding of socio-hydrology. While respecting the strong water conservation culture, weaving different forms of knowledge may help develop a virtuous and original development model in the Algerian Sahara and beyond.

Voting in the Mall: Ideology, Grievance, and Political Consumerism

In this paper we consider how political ideology, attitudes toward historically-marginalized groups, and identification with grievance groups shape Americans’ self-reported participation in political consumerism (i.e., political boycotts and buycotts). Using data from the 2016 and 2020 American National Election Studies (ANES) surveys, we find that ideological intensity has an asymmetrical effect on boycott behavior in 2016, with strong liberals considerably more likely to engage in boycott behavior than strong conservatives. In 2020 the effect of ideological intensity shifts upward dramatically for conservatives, with both liberals and conservatives likely to engage in boycott behavior. We also find mixed results for the effects of attitudes toward and identification with historically-marginalized groups, though we do find that general political participation (and related variables) are strongly related to boycott activity. We discuss the implications of our findings, particularly as they relate to the effects of ideological intensity for liberals and conservatives.

The Causes and Consequences of Public Misunderstanding of Shark Conservation.

Sharks and their relatives face serious conservation challenges. In addition to more effective implementation of regulations already on the books, they need more and stronger conservation and management policies to prevent the extinction of many species, which would have associated negative ecological and economic consequences. Many members of the public are aware of and concerned by shark conservation challenges, but there is widespread misunderstanding of the threats to sharks and the available policy solutions to address those threats. Such misunderstanding has been spread by both well-intentioned but uninformed shark enthusiasts (i.e., people who care and want to help but have limited or incorrect knowledge of key facts and evidence) and also by extremist activist organizations (i.e., those far outside of mainstream norms). Specifically, many members of the public incorrectly believe that the practice of shark finning (and associated demand for shark fins) is the largest or only threat to sharks. In general, the public is far less familiar with widely used and effective tools such as sustainable fisheries management as a solution to shark conservation threats. Many members of the public incorrectly believe that banning the 1% of the global shark fin trade that is the most sustainable will be a major victory for shark conservation. Many members of the public are heavily influenced by information from uninformed extremists rather than from experts. These misunderstandings result in suboptimal policy outcomes, and even conflict between stakeholder groups that ostensibly share goals or desired outcomes. This perspective summarizes a decade of work attempting to understand the causes and consequences of widespread misunderstanding about shark conservation threats and solutions, mapping each along the Science-Policy Interface. It also proposes solutions focusing on sharing our hard-earned expertise with the interested public in an accessible format.

Epitope screening and self-assembled nanovaccine molecule design of PDCoV-S protein based on immunoinformatics.

Based on the whole virus or spike protein of pigs, δ coronavirus (PDCoV) as an immunogen may have unrelated antigenic epitope interference. Therefore, it is essential for screening and identifying advantageous protective antigen epitopes. In addition, immunoinformatic tools are described as an important aid in determining protective antigenic epitopes. In this study, the primary, secondary, and tertiary structures of vaccines were measured using ExPASy, PSIPRED 4.0, and trRosetta servers. Meanwhile, the molecular docking analysis and vector of the candidate nanovaccine were constructed. The immune response of the candidate vaccine was simulated and predicted using the C-ImmSim server. This experiment screened B cell epitopes with strong immunogenicity and high conservation, CTL epitopes, and Th epitopes with IFN-γ and IL-4 positive spike proteins. Ferritin is used as a self-assembled nanoparticle element for designing candidate nanovaccine. After analysis, it has been found to be soluble, stable, non-allergenic, and has a high affinity for its target receptor, TLR-3. The preliminary simulation analysis results show that the candidate nanovaccine has the ability to induce a humoral and cellular immune response. Therefore, it may provide a new theoretical basis for research on coronavirus self-assembled nanovaccines. It may be an effective candidate vaccine for controlling and preventing PDCoV.

Mechanisms of whole body, respiratory, acid-base buffering: a first computer-model test of three physicochemical, acid-base theories.

Acid-base disorders are currently analyzed and treated using a bicarbonate-centered approach derived from blood studies prior to the advent of digital computers, which could solve computer models capable of quantifying the complex physicochemical nature governing distribution of water and ions between fluid compartments. An alternative is the Stewart approach, which can predict the pH of a simple mixture of ions and electrically charged proteins; hence, the role of extravascular fluids has been largely ignored. The present study uses a new, comprehensive computer model of four major fluid compartments, based on a recent blood model, which included ion binding to proteins, electroneutrality constraints, and other essential physicochemical laws. The present model predicts quantitative respiratory acid-base buffering behavior in the whole body, as well as determining roles of each compartment and their species, particularly compartmental electrically charged proteins, largely responsible for buffering. The model tested an early theory that H+ was conserved in the body fluids; hence, when changing Pco2 states, intracellular buffering could be predicted by net changes in bicarbonate and protein electrical charge in the remaining fluids. Even though H+ is not conserved in the model, the theory held in simulated respiratory disorders. Model results also agreed with a second part of the theory, that ion movements between cells and interstitial fluid were linked with H+ buffering, but by electroneutrality constraints, not necessarily by some membrane-related mechanisms, and that the strong ion difference (SID), an amalgamation of ionic electrical charges, was approximately conserved when going between equilibrium states caused by Pco2 changes in the body-fluid system.NEW & NOTEWORTHY For the first time, a physicochemically based, whole body, four-compartment, computer model was used to study respiratory whole body acid-base buffering. An improved approach to quantify acid-base buffering, previously used by this author, was able to determine contributions of the various compartmental fluids to whole body buffering. The model was used to test, for the first time, three fundamental theories of whole body acid-base homeostasis, namely, H+-conservation, its linkage to ion transport, and strong ion difference conservation.

Zebrafish Congenital Heart Disease Models: Opportunities and Challenges.

Congenital heart defects (CHDs) are common human birth defects. Genetic mutations potentially cause the exhibition of various pathological phenotypes associated with CHDs, occurring alone or as part of certain syndromes. Zebrafish, a model organism with a strong molecular conservation similar to humans, is commonly used in studies on cardiovascular diseases owing to its advantageous features, such as a similarity to human electrophysiology, transparent embryos and larvae for observation, and suitability for forward and reverse genetics technology, to create various economical and easily controlled zebrafish CHD models. In this review, we outline the pros and cons of zebrafish CHD models created by genetic mutations associated with single defects and syndromes and the underlying pathogenic mechanism of CHDs discovered in these models. The challenges of zebrafish CHD models generated through gene editing are also discussed, since the cardiac phenotypes resulting from a single-candidate pathological gene mutation in zebrafish might not mirror the corresponding human phenotypes. The comprehensive review of these zebrafish CHD models will facilitate the understanding of the pathogenic mechanisms of CHDs and offer new opportunities for their treatments and intervention strategies.

Ungulates conservation in the face of human development: Mining and roads' influences on habitat and connectivity in Iran's central plateau

Anthropogenic activities such as mining and road construction pose significant threats to ungulate species in the central Iranian plateau. We conducted a comprehensive study focusing on three key ungulates: wild goat (Capra aegagrus), urial (Ovis vignei), and jebeer gazelle (Gazella bennetti). These species rely heavily on migration corridors for functional connectivity, making them vulnerable to the habitat fragmentation caused by human development. Our research integrates ensemble habitat suitability modelling and connectivity analysis to assess the impacts of mining and road construction on the core habitats of these ungulates and their habitat connectivity. Ensemble models predicted large suitable habitats for all three species; however, a considerable portion of these habitats was compromised by the development of mining roads, highlighting the urgency of conservation actions. We found that wild goat habitats were patchy and fragmented, whereas urial and jebeer habitats were more expansive and better connected. Analysis of core habitats identified critical areas for the species survival. For instance, an important core area for wild goats was located in the central part of the landscape, whereas the core habitat for urials was situated in the eastern part. Multiple core areas were identified for jebeer gazelles, highlighting the diverse habitat preferences of the species. We found extensive overlap between the core habitats of the three species and conservation areas (approximately 80%). Connectivity simulations revealed strong conservation network coverage (between 81 and 91%), emphasizing the importance of conservation areas. Our findings underscore the immediate need for conservation interventions to mitigate the impacts of road development on ungulate habitats and migration corridors in central Iran. This research contributes vital information for informed decision-making in conservation planning and sustainable development in the region.

Studying Human Pathogenic Cryptococcus Gattii Lineages by Utilizing Simple Sequence Repeats to Create Diagnostic Markers and Analyzing Diversity.

In this study, we compared the occurrence, relative abundance (RA), and density (RD) of simple sequence repeats (SSRs) among the lineages of human pathogenicCryptococcus gattii using an in-silico approach to gain a deeper understanding of the structure and evolution of their genomes. C. gattii isolate MF34 showed the highest RA and RD of SSRs in both the genomic and transcriptomic sequences, followed by isolate WM276. In both the genomic (50%) and transcriptomic (65%) sequences, trinucleotide SSRs were the most common SSR class. A motif conservation study found that the isolates had stronger conservation (56.1%) of motifs, with isolate IND107 having the most (5.7%) unique motifs. We discovered the presence of SSRs in genes that are directly or indirectly associated with disease using gene enrichment analysis. Isolate-specific unique motifs identified in this study could be utilized as molecular probes for isolate identification. To improve genetic resources among C. gattii isolates, 6499 primers were developed. These genomic resources developed in this study could help with diversity analysis and the development of isolate-specific markers.

Estimating the Aboveground Biomass of Robinia pseudoacacia Based on UAV LiDAR Data

Robinia pseudoacacia is widely planted in the Loess Plateau as a major soil and water conservation tree species because of its dense canopy, complex structure, and strong soil and water conservation ability. The precise measurement of small-scale locust forest biomass is crucial to monitoring and evaluating the carbon sequestration functions of soil and water conservation vegetation. This study focuses on an artificial locust forest planted in the early 1990s in Caijiachuan Basin, Jixian County, Shanxi Province. A drone equipped with LiDAR was used to obtain point cloud data and generate a canopy height model. A watershed segmentation algorithm was used to identify tree vertices and extract individual trees. A relationship model between tree height, diameter at breast height, and biomass, combined with sample survey data, was established to explore the spatial distribution of biomass in the artificial locust forest at the level of the entire basin. The results show the following: (1) the structural parameters of locust extracted using UAV point cloud data have a good degree of fit and accuracy, and the recall rate is 72.7%; (2) the average error rate of the extracted maximum tree height value of locust is 7%, that of the minimum tree height value is 14%, and that of the average tree height value is 18%; (3) the average error rate of the extracted maximum diameter at breast height of locust is 15%, that of the minimum diameter at breast height is 37%, and that of the average diameter at breast height is 36%; and (4) the average error rate of the biomass estimation of locust calculated using point cloud data is 16.0%.

Sturgeon Parasites: A Review of Their Diversity and Distribution

Sturgeon species have inhabited the world’s seas and rivers for more than 200 million years and hold significant taxonomic significance, representing a strong conservation interest in aquatic biodiversity as well as in the economic sector, as their meat and eggs (caviar) are highly valuable goods. Currently, sturgeon products and byproducts can be legally obtained from aquaculture as a sustainable source. Intensive farming practices are accompanied by parasitic infestations, while several groups of parasites have a significant impact on both wild and farmed sturgeons. The present article is a review of common sturgeon parasites from the genus: Protozoa, Trematoda, Crustacea, Nematodes, Monogenea, Hirudinea, Copepoda, Acanthocephala, Cestoda, Polypodiozoa, and Hyperoartia, while also addressing their pathology and statistical distribution.