Abstract In recent years, there has been significant development in microfluidic devices for cell separation and sorting using acoustic methods in biomedical applications. The acoustic interparticle force (AIF) arises from particle interactions with the scattered field of other particles, influencing particle motion at close ranges and facilitating optimal trapping and separation. This study analyzes a two-particle system consisting of a fixed particle and a white blood cell (WBC) within a standing acoustic field and creeping flow using fluid-structure interaction (FSI). To reduce computational costs by decoupling the acoustics and FSI, the acoustic pressure equation (AcPr) was solved on the frequency domain to calculate the total acoustic radiation force in each time step. Model accuracy was assessed by evaluating interparticle (AIF) and primary acoustic radiation forces (ARF) on a polystyrene particle and comparing simulation results to analytical and experimental data. Results demonstrate precise ARF performance, with discrepancies in acoustic interparticle force attributed to viscous losses near the particle surface. Moreover, the higher density of the fixed particle compared to WBCs induces significant acoustic interparticle attraction at close distances. Consequently, cell entrapment occurs through strong attraction and collision with fixed aluminum and silicon particles in creeping flow in all three Reynolds numbers 1.4E-3, 2.1E-3, and 3E-3. Increasing Reynolds numbers augment the likelihood of cell separation from the fixed particle. These findings contribute to optimizing cell isolation and entrapment strategies.
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