Strong Cation Exchange Resin Research Articles

Analysis of 2-aminopyridine labeled glycans by dual-mode online solid phase extraction for hydrophilic interaction and reversed-phase liquid chromatography

Quantitative analysis of glycans released from glycoproteins using high-performance liquid chromatography (HPLC) requires fluorescent tag labeling to enhance sensitivity and selectivity. However, the methods required to remove large amounts of excess labeling reagents from the reaction mixture are time-consuming. Furthermore, these methods, including solvent extraction and solid phase extraction (SPE), often impair quantitative analysis. Here, we developed an online sample cleanup procedure for HPLC analysis of 2-aminopyridine (AP)-labeled glycans using a six-port/two-way valve and two small columns: one packed with a strong cation exchange resin (SCX) and the other comprising ODS silica gel. AP-labeled glycans delivered from an injection port were separated from excess AP by passing through an SCX column (4.6 mm i.d., 1 cm long) regulated to 40°C. The AP-labeled glycans were trapped on an ODS column (4.6 mm i.d., 1 cm long) to further separate them from inorganic contaminants. By changing the valve position after 2 min to connect the ODS column to an analysis column, AP-labeled glycans trapped in the ODS column were eluted with an acetonitrile-containing eluent followed by hydrophilic interaction liquid chromatography (HILIC) separation on an amide column or reversed-phase mode separation on a C30 column. This method was successfully used to analyze N-linked glycans released from several glycoprotein samples.

A process combination of ion exchange and electrodialysis for the recovery and purification of hydroxy acids from secondary sources

Ion exchange and electrodialysis processes for the production of hydroxy acids (HAs) from alkaline soda and kraft black liquors (BLs) were investigated. Ion exchange with a type-2 strong anion exchange resin in sulfate form was used to separate thiosulfate from alkaline kraft-BL-based mixture of sodium (Na+) salts of HAs. Efficient regeneration of the spent resin to sulfate form was achieved only through hydroxide form. Ion exchange with strong cation exchange (SAC) resins was successfully used to convert the Na+ salts of HAs in soda-BL-based solution and thiosulfate-free kraft-BL-based solution into free acids with complete removal of Na+ ions from the solution. The spent resin was efficiently regenerated with 1–2 mol/L sulfuric acid (H2SO4). Electrodialysis with bipolar membranes (EDBM) was used for the generation of H2SO4 and sodium hydroxide (NaOH) from the spent SAC resin regenerants (sodium sulfate–sulfuric acid mixtures). Overall, 1 mol/L H2SO4 with 95% purity and 90% yield could be obtained, but excess water flux through the membranes limited the maximum obtainable H2SO4 concentration to 1.17 mol/L. Simultaneously, 0.79 mol/L NaOH was produced with 93% purity. EDBM-based H2SO4 was successfully used for the regeneration of the SAC resin. With EDBM, a closed loop can be built for the regeneration of SAC resin with the simultaneous generation of NaOH. The results of this study demonstrate that a process combination of ion exchange and electrodialysis decreases the overall chemical consumption related to HA production and enables efficient chemical recycling.

Activity and stability of urease enzyme immobilized on Amberlite resin

Abstract Immobilization of enzymes is a good field of study to extend the life of enzyme and reduce the cost of the chemical processes, such as separation processes. Urease is an important enzyme with medical and industrial applications. The aim of the present study is to prepare an immobilized urease on a strong cation exchange resin (Amberlite IR120 Na) and study its activity and stability. We monitored the release of Na ions in the collected fractions and searching for enzyme in the fractions as indicators of immobilization by ion exchange phenomenon. Sodium is determined by using atomic absorption spectroscopy technique, while the enzyme concentration was tested by Bradford’s method. Immobilized urease activity was evaluated by salicylate-hypochlorite method. The results indicated a complete immobilization of urease enzyme on the resin surface with reserving 92% of the activity of free enzyme. The immobilized urease enzyme on resin showed good stability and it has a 62% of its activity after 154 days of storage at room temperature. It is concluded that a new immobilized urease enzyme system is prepared with good enzyme activity and stability.

Recycle of vanadium from aluminum slag of ferrovanadium

Vanadium is a rare chemical element and it primarily comes from the by-product of other minerals. In this study, the material source mainly comes from vanadium-rich aluminum slag. This experiment aims to separate vanadium and other impurities, such as aluminum and zirconium. First, the slag was dissolved in the sodium hydroxide solution at pH value 13, and part of the alumina was precipitated. After removing the precipitation, the strong acid cation exchange resin, Dowex® G26, was added to eliminate the remaining impurities. In this procedure, the parameter of reaction time, the mass of ion exchange resin and pH value were supplied. Moreover, the adsorption isotherms described by means of the Langmuir and Freundlich isotherms were used to investigate the ion-exchange behaviors of vanadium. To sum up, this study will provide the best conditions of ion exchange to get high purity about 95% of vanadate.

P.113 The nitric oxide donors nebivolol and S-nitroso-glutathione decrease the spontaneous motor behaviour in rats

In search for new enantioselectivity profiles, the N-decyl-S-trityl-(R)-cysteine [C10-(R)-STC] was synthesized through a one-step procedure and then hydrophobically adsorbed onto an octadecylsilica surface to generate a stable chiral stationary phase for ligand-exchange chromatography (CLEC-CSP) applications. The CLEC analysis was carried out on underivatized amino acids, by using a Cu(II) sulphate (1.0 mM) containing aqueous eluent system. Most of the analysed compounds (34 out of 45) were enantiodiscriminated by the C10-(R)-STC-based CSP, with resolution factor (RS) values up to 8.86. Conformationally rigid and hydrophobic ligands often experienced the largest enantioselectivity effects. A high loadability emerged from the analysis of rac-NorVal (selected as prototype test compound): up to 20 mg/mL were efficiently enantioseparated with the CLEC-CSP. Two in-line hand-made cartridges filled with a strong cation-exchange resin allowed the effective catching of Cu(II) ions after the semi-preparative enantioseparation. The quantitative recovery of the rac-NorVal enantiomers was made possible by flowing through the cartridge a 5% (v) ammonia solution. The CLEC phase proved successful in the enantioselective analysis of a commercially available (S)-Leu containing tablet. Furthermore, in order to understand the molecular basis for a successful use of the C10-(R)-STC-based CLEC system, a descriptive structure-separation relationship study was performed. As a result, all compounds with a MEAN-QPlogS (a hydrophilicity descriptor) value lower than 0.373 can be most likely enantioseparated with the CLEC system under investigation. In the work, the numerous aspects complying with the principles of green chromatography are highlighted and discussed.

Selective Separation of Radium and Actinium from Bulk Thorium Target Material

A complete scheme for the selective separation of actinium and radium isotopes from bulk 232Th target material will be presented. The process may be applicable to the production of 225Ac and 227Ac via proton spallation on thorium targets. Thorium metal is dissolved in sulfuric acid with small amounts of HF. Actinium and radium are retained on cation exchange resin from the sulfate medium, while neutral and anionic thorium sulfate complexes are rejected. Targeting the minor components (Ac,Ra) allows use of much smaller chromatographic columns than traditional nitrate based systems which target the bulk thorium. Additionally, the primary separation is performed from 0.1M Th, 0.6M sulfate, pH 1-2 instead of the more corrosive 3-8M HNO3 typically employed in solvent extraction or anion exchange based thorium separations. Actinium and radium are recovered from the cation exchange resin in a small volume of 5M HNO3 and directly purified via extraction chromatography with UTEVA and DGA resins without feed adjustment or evaporation. The radium fraction can be further processed following ingrowth of 225Ac from 225Ra to produce additional 225Ac free from any potential 227Ac impurity. The flowsheet has been tested at the Canadian Nuclear Laboratory (CNL) on 25 gram solid thorium targets irradiated at Fermi Lab. -Bond, A.H., Horwitz, E.P., McAlister, D.R., 2006. A Multicolumn Selectivity Inversion Generator for the Production of High Purity Actinium-225 for Use in Therapeutic Nuclear Medicine, United States patent number 7,087,206. (Licensed to NorthStar Medical Radioisotopes) -D.R. McAlister, E.P. Horwitz, “Selective Separation of Radium and Actinium from Bulk Thorium Target Material on Strong Acid Cation Exchange Resin from Sulfate Media,” Applied Radiation and Isotopes, 140, 18-23 (2018).

Superhigh adsorption of nickel from electroplating wastewater by raw and calcined electroplating sludge waste

Abstract Both the raw electroplating sludge (RES) waste and the calcined electroplating sludge (CES) were utilized to adsorb nickel from the real electroplating wastewater. The CES was obtained via calcination treatment at 500 °C, and the CES and RES were characterized by X-ray fluorescence (XRF), Fourier transform infrared spectrometer (FT-IR), X-ray powder diffraction (XRD). Adsorption isotherm results showed that the maximum adsorption capacities of Ni2+ on the CES and RES were 163.6 and 210.9 mg/g according to the Langmuir fitting, respectively. In the real nickel-containing wastewater treatment, the RES exhibited much higher adsorption capacity for Ni2+ than commonly used adsorbents including coal-based activated carbon (CAC), strong acid cation-exchange resin (D001), weak acid cation-exchange resin (D113) and amino carboxylic acid chelating resin (D463). The adsorption mechanisms of Ni2+ on the RES and CES mainly included ion exchange and surface complexation. The large amount of Ca2+ in the adsorbents played an important role in the adsorption of Ni2+ via cation exchange, and the hydroxyl and carboxyl groups on the adsorbent were also responsible for Ni2+ adsorption. Therefore, the electroplating sludge waste is an efficient adsorbent for Ni2+ removal from high concentrations of nickel-containing electroplating wastewater. This study provides a new method for the utilization of electroplating sludge as efficient adsorbents before being treated as hazardous waste, showing the feasible concept of disposal waste with waste.

Rapid sample clean-up procedure for aminophosphonate determination by LC/MS analysis

Aminophosphonates are commonly utilised for complexing bivalent ions such as calcium and magnesium. With regard to environmental samples, the analysis of these highly polar compounds is still challenging due to matrix effects and lacking analytical standard methods. We have recently developed a LC/MS method for common aminophosphonates without derivatisation. This LC/MS method delivers precise and accurate measurement for standard samples with very low concentration of cations disturbing the analysis. However, due to matrix effects this LC/MS method requires a sample clean-up being also applicable to natural water or wastewater samples. We developed and optimised a sample clean-up procedure applying strong cation exchange resin Dowex 50WX8. This clean-up allows a single LC/MS analysis of hydroxyethelidene(diphosphonic acid) (HEDP), aminotris(methylenephosphonic acid) (ATMP), ethylenediaminetetra(methyloenephosphonic acid) (EDTMP), diethylenetriaminepenta(methylenephosphonic acid) (DTPMP) and its major intermediates amino(methylphosphonic acid) (AMPA) and iminodi(methylenephosphonic acid) (IDMP). We compared different test conditions with six aminophosphonates in either ultra pure water or tap water. The latter was used to simulate cation concentrations typical for natural waters. To elute all aminophosphonate including AMPA, ammonium acetate addition was necessary. The addition of ammonium acetate was combinable with all tested aminophosphonates and provides high sample quality for LC/MS analysis. For acceptable recovery, the smallest aminophosphonate AMPA required the highest addition of ammonium acetate (1000 mg L-1) during the sample clean-up. Finally, the optimised clean-up procedure was successfully applied to identify and quantify phosphonates from an industrial wastewater sample. The sample clean-up procedure is simple, cheap, rapid and precise and can be further combined with solid phase extraction and more sensitive LC/MS methods.

Study on Methyl Esterification of Salicylic Acid Using an Intensified Fixed Bed Reactor

Abstract Methyl esterification of salicylic acid catalyzed by strong acidic cation exchange resin NKC-9 was carried out on an intensified fixed bed reactor (IFBR) to investigate the effects of different parameters and the results showed that the optimal conditions were as follows: circulation speed is 1.5 L· h−1, catalyst loading is 20 %, initial mole ratio of salicylic acid and methanol is 1:6 and reaction temperature is 343.15 K. The thermodynamics for the methyl esterification of salicylic acid have been studied to obtain the equilibrium constant from the experimental data at different temperature. PH, E-R and LHHW models were used to correlate the kinetic data in the temperature range from 328.15 to 348.15 K. The calculated value is in good agreement with experimental value, indicating that all the models can be used to accurately describe the process of the methyl esterification of salicylic acid.

Rapid Separation and Precise Determination of Strontium Isotopic from Geological Samples with High Rubidium/Strontium Ratios

Abstract In the isotopic measurement of strontium (Sr) of geological samples with high rubidium (Rb)/Sr ratios, it is difficult to completely remove the isobaric interference of Rb on Sr isotopic analysis by a single separation and purification using traditional strong acid cation exchange resin. Actually, one purification procedure performed twice is necessary to eliminate the interference of 87Rb on the testing of 87Sr/86Sr. Although the purification procedure can ensure a good effect on the separation and purification of Sr, the process is tedious and time-consuming, which is not suitable for simultaneous separation and purification of Sr from large quantity of geological samples with high Rb/Sr ratios. In this study, the conventional two-stage separation process was optimized, and an experimental method for rapid separation of Sr from geological samples with high Rb/Sr ratios was established. In this method, the Sr solution after purified by AG50 resin was directly added to a new AG50 resin to purify Rb and Sr again. The whole separation process would be accomplished quickly and efficiently. The method combined with highly sensitive thermal ionization mass spectrometer (TIMS) technique was completely satisfied in high precision determination of 87Sr/86Sr isotope ratio in the samples with high Rb/Sr ratios. Through the application of the novel separation method with a large number of geological samples with high Rb/Sr ratios, it was proved that the experimental process was stable and reliable and showed crucial application value.

Unexpected irregular structures of poly(itaconic acid) prepared in Deep Eutectic Solvents

Poly(itaconic acid) (PIA) was synthesized by persulfate initiated free-radical polymerization of ternary Deep Eutectic Solvents (DES) containing equimolar amounts of itaconic acid (IA) as hydrogen-bond donor, quaternary ammonium salt, namely choline chloride (CC) or tetraethylammonium chloride (TEAC) as hydrogen-bonds acceptors, and water as the initiator solvent. The syntheses were carried out at 65 °C for 2 h, and polymers produced were purified by dialysis and by treatment on strong cation exchange resin. The microstructures of the polymers were analysed using 1H, 13C and 1H–13C HSQC NMR spectroscopy. The unexpected methylene and methine groups resulting from decarboxylation and/or branching as well as choline ester moieties were detected as irregular structures in PIA synthesised in IA-TEAC and IA-CC DES, respectively.

Biodiesel production using gel-type cation exchange resin at different ionic forms

In this study, a strong acidic-type cation exchange resin was used in the transesterification of corn oil to fatty acid methyl esters (FAME). The gel-type cation exchange resin (Purolite-PD206) was used in H+ and Na+ forms to utilize ion-exchange resin as effective heterogeneous catalyst in the production of biodiesel. Effect of ionic forms of ion exchange resin on free fatty acid (FFA) conversion and composition was investigated by using different amounts of ion exchange resin (12, 16, and 20 wt%), various mole ratios of methanol to oil (1:6, 1:12, and 1:18 mol/mol), reaction temperatures (63, 65, and 67°C), and reaction time (24, 36, and 48 h) during transesterification reaction. The highest FFA conversions of 73.5% and 79.45% were obtained at conditions of 20 wt% of catalyst, 65°C of reaction temperature, 18:1 as methanol to oil ratio, and 48 h of reaction time for H+ and Na+ forms of ion exchange resin, respectively. These results were obtained from regression equations established by using analysis of variance (ANOVA) model according to the experimental results of selected parameters. Gas chromatography analysis revealed that FAME is mainly composed of C16:0 (palmitic), C18:1 (oleic), and C18:2 (linoleic) acids of methyl ester.

Selective Separation of Radium and Actinium from Bulk Thorium Target Material

A complete scheme for the selective separation of actinium and radium isotopes from bulk 232Th target material will be presented. The process may be applicable to the production of 225Ac and 227Ac via proton spallation on thorium targets. Thorium metal is dissolved in sulfuric acid with small amounts of HF. Actinium and radium are retained on cation exchange resin from the sulfate medium, while neutral and anionic thorium sulfate complexes are rejected. Targeting the minor components (Ac,Ra) allows use of much smaller chromatographic columns than traditional nitrate based systems which target the bulk thorium. Additionally, the primary separation is performed from 0.1M Th, 0.6M sulfate, pH 1-2 instead of the more corrosive 3-8M HNO3 typically employed in solvent extraction or anion exchange based thorium separations. Actinium and radium are recovered from the cation exchange resin in a small volume of 5M HNO3 and directly purified via extraction chromatography with UTEVA and DGA resins without feed adjustment or evaporation. The radium fraction can be further processed following ingrowth of 225Ac from 225Ra to produce additional 225Ac free from any potential 227Ac impurity. The flowsheet has been tested at the Canadian Nuclear Laboratory (CNL) on 25 gram solid thorium targets irradiated at Fermi Lab. -Bond, A.H., Horwitz, E.P., McAlister, D.R., 2006. A Multicolumn Selectivity Inversion Generator for the Production of High Purity Actinium-225 for Use in Therapeutic Nuclear Medicine, United States patent number 7,087,206. (Licensed to NorthStar Medical Radioisotopes) -D.R. McAlister, E.P. Horwitz, “Selective Separation of Radium and Actinium from Bulk Thorium Target Material on Strong Acid Cation Exchange Resin from Sulfate Media,” Applied Radiation and Isotopes, 140, 18-23 (2018).

Adsorption characteristics of benzene on resin-based activated carbon under humid conditions

VOCs removal under humid conditions is an important issue in the various industries. However, adsorption capacity of commercial coconut-shell–based activated carbon (CAC) is diminished considerably in high relative humidity. In this study, we prepared resin-based activated carbon (RAC) from strong cation-exchange resins consisting of polystyrene with divinylbenzene, and investigated benzene adsorption characteristics under humid conditions. The results of water isotherm and breakthrough experiments revealed that RAC adsorbed no water vapor in low P/P0 region and the amount of adsorbed benzene did not decrease significantly with the addition of water vapor, indicating high water resistance compared to CAC. This high resistance of RAC to water vapor can be contributed by the low content of hydrophilic sites (metal impurity and surface oxygen), confirmed by XPS and ICP results. The relationship between RAC porosity and benzene adsorption under humid conditions was also investigated. Benzene adsorption under humid conditions was influenced significantly by the narrow micropore volume of RAC. As the narrow micropore volume of RAC decreased, the adsorption of water vapor was inhibited, so that the decline in the time of breakthrough at relative humidity at 70% was considerably alleviated from 44 to 1.2%.

Adsorption of rare earth elements by strong acid cation exchange resin thermodynamics, characteristics and kinetics

The equilibrium and kinetic characteristics of rare earths adsorption process using the strong acid macro reticular resin Dowex 50X8 from El-Erediya chloride liqueurs have been determined. The adsorption parameters including pH, contact time, temperature, resin to aqueous ratio and rare earths initial concentration in aqueous have been optimized. The practical adsorption capacity of rare earths upon the resin under the optimum conditions has been found to attain 80 mg/g which matches with Langmuir theoretical capacity. The physical parameters including the adsorption kinetics, the isotherm models and the thermodynamic data have also been determined. The adsorption process has been found to agree with both the pseudo second order reaction, the Langmuir isotherm and confirms with the concept of the matrix diffusion controls. A successful preconcentration process for the rare earths content was done with a preconcentration factor of about 42.33. Considerable rare earths cake was precipitated from the chloride eluate solutions using oxalic acid.

Continuous synthesis of isobornyl acetate catalyzed by a strong acid cation exchange resin in an oscillatory flow reactor

Recently, the application of continuous reaction technology for the production of fine chemicals has expanded significantly. Despite the increasing interest in continuous flow manufacturing, most industries continue to use multipurpose batch or semi-batch reactors. In this work, the utilization of a continuous oscillatory flow reactor(OFR) for heterogeneous catalysis is demonstrated by conducting a typical acetylation reaction (the acetylation of camphene) Parallel experiments was also performed in a batch stirred tank reactor (BSTR), and the results were compared with those obtained in the OFR. The temperature inside the OFR was uniform than that inside the BSTR, and the residence time distribution in the former system was close to that in a plug flow reactor. In addition, the OFR exhibited higher reaction rate and selectivity values than those obtained for the BSTR. Although the liquid was refreshed faster inside the OFR and no polymer residue was formed on the catalyst surface, its application required the screening of catalysts grains, which increased the probability of surface cracking. The OFR for continuous flow catalysis can serve as a promising starting point in the development of new, low-cost, and more efficient reactors for the synthesis of pharmaceuticals and other fine chemicals.

Sugarcane bagasse mild alkaline fractionation and production of purified fractions by pulse chromatography with water

Sugarcane bagasse (SCB) was treated under mild alkaline conditions (solid:liquid ratio of 1:20 (w/v), 1.5% NaOH (w/v), 60 °C, 6 h) to fractionate the lignocellulose in order to produce a typical mild alkaline extract from a lignocellulosic biomass. The solid residue was enriched in cellulose, while the SCB alkaline extract contained lignin and hemicelluloses, but also inorganic salts, five phenolic monomers and acetic acid. After concentration of the alkaline extract by evaporation, low pressure chromatography with water as eluent was performed to produce purified fractions. Two different strong acid cation (SAC) exchange resins were tested: one gel-type resin and one macroporous-type resin. The lignin and hemicelluloses were separated from the inorganic salts by the gel-type SAC exchange resin. On this resin, the phenolic monomers were partitioned regarding the presence or absence in their structure of a carboxyl group. On the macroporous-type SAC exchange resin, the largest sugar oligomers and lignin oligomers were obtained in a fraction free of inorganic salts, phenolic monomers and acetic acid.

Sorption of Fluoride Ions by Iron Oxyhydrate Fixed on the Carriers Part I. Organic Carriers

The possibility of using an inorganic sorbent of iron oxyhydrate (IOH) for the removal of F– ions from process solutions of zinc production is considered. The synthesis method of IOH is chosen. The results of scanning electron microscopy and X-ray phase analysis are presented. The possibility in principle of using ion-exchange resins as carriers modified by IOH is considered. The formation of an active substance on anion-exchange and cation-exchange resins is investigated. It is shown that the strongest composite sorbents are formed when using strong acidic cation-exchange resins with sulfonate groups. The method of IOH incorporation into the structure of carrier materials and the formation of composite sorbents is described. The KU-2×8 cation exchanger is recommended as the composite basis. To form IOH crystals of the β-modification distributed over the ion-exchanger grain bulk, iron-saturated cation exchangers are held in the sodium chloride solution with a concentration of 2.5 g/dm3 for 24 h at 85°C. Anion exchangers are held in the iron(III) sulfate solution with the sodium chloride additive for 24 h at t = 85°C. Herewith, the formation of IOH films on the surface of sorbent grains is observed. The fluorine was sorbed in a static mode from a model solution with the concentration of F– = 100 mg/dm3 at t = 60°C. The sorption on the AV-17×8 anion exchanger is performed at t = 20°C. Adsorbed fluorine is desorbed by the 0.1 M NaOH solution at t = 60°C for 2 h. The synthesized KU-2×8-IOH composite sorbent has a capacity of 0.7–1.1 mg/g with respect to fluorine and can be regenerated with the formation of easily utilizable fluorine-containing eluate.

Application of ion-exchange resin as solid acid for buffer-free production of γ-aminobutyric acid using Enterococcus faecium cells

In this study, ion-exchange resin was applied as solid acid catalyst for the γ-aminobutyric acid (GABA) production using a strain of Enterococcus faecium (GZ2) by whole-cell bioconversion. It was observed that the accumulated GABA resulted in the inhibition of the bioconversion activity. To improve the productivity, a cation-exchange resin-based in situ product removal (ISPR) approach was developed. Three cation-exchange resins were tested to select a suitable adsorbent used in the bioconversion. The strong acidic cation-exchange resin IRP-69 exhibited the highest GABA production and remained helpful in reducing the free GABA concentration in the reaction solution. Compared to the bioconversion in sodium acetate buffer, the GABA production, with the addition of resin, was significantly improved by 2.12-fold. Furthermore, a fed-batch bioconversion strategy with the feeding of L-monosodium glutamate (L-MSG) was tested. The maximum production of GABA, 406.1 mM, was achieved after 12 h reaction with an initial substrate concentration of 200 mM, and a triple feeding of 100 mM L-MSG afterwards at 4 h, 6 h and 8 h. The result shows that the operation of bioconversion reaction in a feeding substrate batch mode is an effective approach to improve the efficiency and productivity of GABA.

A simple dispersive solid phase extraction clean-up/concentration method for selective and sensitive quantification of biogenic amines in wines using benzoyl chloride derivatisation

A simple, quick, cheap and green dispersive solid phase extraction (dSPE) method followed by benzoyl chloride pre-column derivatisation for HPLC-UV determination of twelve biogenic amines (BAs) in wines is proposed for the first time. The dSPE using a strong cation exchange resin increased the selectivity and sensitivity of the analysis by elimination of interfering compounds and a five-fold enrichment of BAs. The method presented an adequate precision and linearity with detection limits ranging from 0.133 to 0.509 mg/L. Recoveries ranging from 72 to 99% prove the accuracy of the method for determining BAs in red, white and Tawny Port wine samples yielding chromatograms clean from interferents. The method was applied successfully to the analysis of 31 young red wines from different Portuguese wine regions. The dSPE method although has a potential of broader application to other food matrixes, other derivatisation procedures than benzoyl chloride and other detectors.