A piezoelectric microgravimetry (PM) chemosensor, featuring a film of molecularly imprinted polymer (MIP) of poly[bis(2,2′-bithienyl)methane] bearing either a 3,4-dihydroxyphenyl or benzo-18-crown-6 substituent, for selective determination of dopamine was devised and tested. A Pt/quartz resonator and a dopamine-templated MIP film, deposited by electropolymerization onto an underlayer of poly(bithiophene), served as the transducer and recognition element of the chemosensor, respectively. The UV–vis spectroscopic and XPS as well as electrochemical measurements verified completeness of the dopamine template extraction with a strong base solution. The extraction-generated molecular cavities featured recognition sites that served selective dopamine analyte binding. The SECM imaging substantiated the permeability characteristics of the template-free MIP film. The dopamine analyte was determined under FIA conditions with the PM detection. The lower limit of detection was 10 nM dopamine at favorable conditions involving the 35 μL/min carrier solution flow rate and the injected sample volume of 1 mL. The sensitivity of the chemosensor increased almost fivefold when the poly(bithiophene) film coated Pt/quartz electrode was used instead of the bare Pt/quartz electrode as the substrate for deposition of the MIP film. The chemosensor successfully discriminated dopamine from structural and functional analogues, such as 2-phenylethylamine, histamine, and ascorbic acid. The optimum mean thickness of the MIP film was ∼ 220 nm.