• The incorporation of anti- Stripping in hot mix asphalt was investigated. • The effects of anti- Stripping agents on the specimens by image processing were evaluated. • The performance of specimens under different working conditions was analyzed. • Anti-stripping agent improved of adhesive property of HMA asphalt mixtures. Infiltration of water into Hot Mix Asphalt (HMA) can cause performance degradation and service life reduction of the asphalt concrete pavements. Moreover, it can lead to adhesion failure at the binder-aggregate interface and/or cohesion failure within the binder or binder-filler mastic. In this paper, three aggregate types, three anti-stripping additives, and one bitumen type were selected to study this phenomenon. Based on the results of chemical analysis, selected aggregates were categorized into two groups: non-stripping and stripping aggregates. Then, the stripping propensity of loose and compacted mixes was assessed through boiling water, retained compressive strength, and indirect tensile tests. The chemical analysis was showed the importance of amphoteric and alkaline oxides in moisture sensitivity in silicate rocks. The results emphasize the detrimental effect of moisture in HMA mixes even in the non-stripping mix. All selected anti-stripping additives improve the tensile strength ratio (TSR) of specimens, however, the stripping propensity of some treated mixes was maintained. In the next step, cohesion loss and adhesion loss surfaces of loose mixes and ITS specimens was determined under the wet conditions by the image processing technique in ENVI and MATLAB software packages. Based on image processing results, the higher retained bitumen coating in loose mixes leads to less stripping propensity of compacted specimens and the tensile strength ratio of modified Lottman specimens is decreased by an increase in the percentage of broken aggregates and adhesion loss. Image processing data were subjected to nonlinear regression and multiple analysis of variance (MANOVA) to investigate this phenomenon.