Striking Pattern Research Articles

Vasculitis of the gallbladder: a case series of an unusual pattern of cholecystitis

Vasculitis isolated to the gallbladder is uncommon, with few cases reported in the literature. We present a series of nineteen cases of cholecystitis with associated vasculitis. The male to female ratio was 7:12 and average age 75.6 (range 49–97). Most resections were performed for the clinical or imaging impression of cholecystitis, with two removed at the time of a larger resection for malignancy elsewhere. The majority (18/19) of cases showed venous involvement (phlebitis), sparing arteries. Most of the vasculocentric infiltrates were lymphohistiocytic, with five cases showing granulomatous vasculitis. The background mucosal inflammatory pattern in just over half (11/19) of cases showed a lymphofollicular cholecystitis, with the remainder showing typical chronic cholecystitis (6/19) or active chronic cholecystitis (1/19). Two of the cases (2/19) showed striking intraepithelial lymphocytosis. Eight of the samples were acalculous. It is unclear why this pattern occurs, with no patients in this series having a known history of vasculitis, and no association with IgG4 or autoimmune disease identified. The pattern may represent an unusual response to local mucosal injury or to constituents/metabolites in bile. The clinical significance is uncertain. The purpose of this case series is to contribute to the sparse literature describing this striking but uncommon histological reaction pattern.

Competition and cooperation in artificial intelligence standard setting: Explaining emergent patterns

AbstractEfforts to set standards for artificial intelligence (AI) reveal striking patterns: technical experts hailing from geopolitical rivals, such as the United States and China, readily collaborate on technical AI standards within transnational standard‐setting organizations, whereas governments are much less willing to collaborate on global ethical AI standards within international organizations. Whether competition or cooperation prevails can be explained by three variables: the actors that make up the membership of the standard‐setting organization, the issues on which the organization's standard‐setting efforts focus, and the “games” actors play when trying to set standards within a particular type of organization. A preliminary empirical analysis provides support for the contention that actors, issues, and games affect the prospects for cooperation on global AI standards. It matters because shared standards are vital for achieving truly global frameworks for the governance of AI. Such global frameworks, in turn, lower transaction costs and the probability that the world will witness the emergence of AI systems that threaten human rights and fundamental freedoms.

Influences of altering footstrike pattern and cadence on lower extremity joint coordination and variability among runners with patellofemoral pain.

Patellofemoral pain (PFP) is a common overuse injury among runners. It is not only a hindrance to the runner's training, but also to the runner's quality of life. PFP runners may strategize different running strategies to reduce patellofemoral joint stress, release pain, and improve function. This study aimed to determine the changes in joint coordination and variability under combinations of foot strike pattern and cadence for runners with patellofemoral pain. Twenty male runners with PFP performed six running strategies which were two strike patterns named forefoot (FFS) and rearfoot (RFS) accompanied by three running cadences named slow10%, normal, and fast10%. A modified vector coding technique and circular statistics were respectively used to identify the coordination pattern and variability between hip sagittal-knee frontal (HsKf), hip sagittal-knee sagittal (HsKs) and knee transverse-ankle frontal (KtAf) during stance phase. Coordination patterns which were conformed with anatomical motion pattern was classified as mechanically sound, and the distribution frequency of each coordination pattern was quantified. Switching to FFS, the HsKf couples (p < 0.001, ES = 1.34) and the HsKs couples (p = 0.001, ES = 0.82) displayed significantly greater frequency in mechanically unsound coordination pattern during the initial stance phase. The effect of increasing running cadence on RFS displayed significantly greater frequency in mechanically unsound hip dominancy (p = 0.042, ES = 0.65) and knee dominancy (p = 0.05, ES = 0.70) coordination patterns for HsKf couples as well as for HsKs couples (p = 0.023, ES = 0.86) during the initial stance phase. Combined with FFS and fast10% cadence, HsKs couples showed more hip-dominated mechanical sound coordination pattern (p = 0.002, ES = 1.25). Further, altering footstrike pattern and cadence failed to change the coordination variability. Changing running cadence (± 10%) combined with transfer strike pattern from RFS to FFS could not increase the distribution frequency in mechanically sound coordination patterns and change coordination variability for PFP runners.

A food insecurity Kuznets Curve?

Advances in food security proceed unevenly within and across nations. A striking pattern emerges from analysis of >560,000 individual responses to the first globally comparable, nationally representative, repeated food insecurity survey, which is statistically representative of >96 % of the world’s population. We find the relationship between the prevalence of food insecurity in a country and intranational, interpersonal inequality in food insecurity follows a strong inverse-U shape, i.e., a Kuznets Curve. The relationship is stable over time and across relevant inequality measures and estimation methods. This finding can help guide the implementation of safety nets and social protection programs to achieve the UN’s Sustainable Development Goal 2 and to satisfy the human right enshrined in Article 25 of the 1948 Universal Declaration of Human Rights.

Effectiveness of Lower-Cost Strategies for Running Gait Retraining: A Systematic Review

The effectiveness of lower-cost equipment used for running gait retraining is still unclear. The objective of this systematic review was to evaluate the effectiveness of lower-cost equipment used in running gait retraining in altering biomechanical outcomes that may be associated with injuries. The literature search included all documents from MEDLINE, Web of Science, CINAHL, SPORTDiscus, and Scopus. The studies were assessed for risk of bias using an evaluation tool for cross-sectional studies. After screening 2167 initial articles, full-text screening was performed in 42 studies, and 22 were included in the systematic review. Strong evidence suggested that metronomes, smartwatches, and digital cameras are effective in running gait retraining programs as tools for intervention and/or evaluation of results when altering step cadence and foot strike patterns. Strong evidence was found on the effectiveness of accelerometers in interventions with feedback to reduce the peak positive acceleration (PPA) of the lower leg and/or footwear while running. Finally, we found a lack of studies that exclusively used lower-cost equipment to perform the intervention/assessment of running retraining.

Living fossils and conservation values

Horseshoe crabs (Limulus polyphemus) have been in decline in Long Island Sound, and recently there has been discussion of whether the state of Connecticut should stop issuing licenses for commercial harvesting. This paper argues that in spite of concerns about the living fossil concept, the fact that the horseshoe crabs are living fossils should count in favor of more stringent protection. The paper distinguishes four different views about the status of the living fossil concept: 1) eliminativism; 2) redefinition; 3) reframing; and 4) conceptual pluralism. Approaches 2–4 all treat the criteria associated with living fossils as picking out distinctive features of evolutionary history. Those distinctive features of evolutionary history link up with conservation values in several ways. More generally, drawing upon relevant work in environmental philosophy, it is argued that evolutionary history is relevant to aesthetic and environmental value. Moreover, eliminativists have trouble rendering intelligible a striking pattern in the recent scientific literature. Researchers undertaking conservation-relevant work frequently highlight the living fossil status of the taxa under study.

Atlantic sturgeon and shortnose sturgeon exhibit highly divergent transcriptomic responses to acute heat stress

In comparison to most modern teleost fishes, sturgeons generally display muted stress responses. While a muted stress response appears to be ubiquitous across sturgeon species, the mechanisms unpinning this muted response have not been fully described. The objective of this study was to determine the patterns of hematological and transcriptomic change in muscle tissue following an acute high temperature stress (critical thermal maxima; CTmax) in two locally co-occurring but evolutionarily distant sturgeon species (Atlantic and shortnose sturgeon). The most striking pattern found was that Atlantic sturgeon launched a vigorous transcriptomic response at CTmax, whereas shortnose sturgeon did not. In contrast, shortnose sturgeon have significantly higher cortisol than Atlantics at CTmax, reconfirming that shortnose have a less muted cortisol stress response. Atlantic sturgeon downregulated a number of processes, included RNA creation/processing, methylation and immune processes. Furthermore, a number of genes related to heat shock proteins were differentially expressed at CTmax in Atlantic sturgeon but none of these genes were significantly changed in shortnose sturgeon. We also note that the majority of differentially expressed genes of both species are undescribed and have no known orthologues. These results suggest that, while sturgeons as a whole may show muted stress responses, individual sturgeon species likely use different inducible strategies to cope with acute high temperature stress.

Syntactic complexity in legal translated texts and the use of plain English: a corpus-based study

This study compared the linguistic difficulty of legal translated texts with the syntactic complexity of native English legal writings in order to demonstrate the statistically significant differences between the two big datasets. The study applies features of the syntactic complexity of sentences within legal text translations that translated texts are less complicated than their original counterparts. It provides an example of how easy plain English translation in legal communication might result in understandable target writings. The findings of the legal translation of the people of three regions reveal striking patterns in terms of syntactic complexity and legal communication in plain English, which are consistent with previous research. Complex nominal and hypotactic structures result in a high number of propositions per sentence, placing a high demand on the cognitive processing abilities of those who read and understand the text. The statistics show considerable differences among the three locations and various forms of company law corpora. The study is the first large-scale quantitative analysis of the accessibility of legal jargon compared to other forms of English, emphasizing the efficacy of plain-language initiatives in legal translations.

Maternal effects and the legacy of extreme environmental events for wild mammals.

Maternal effects and the legacy of extreme environmental events for wild mammals.

VISTA: visualizing the spatial transcriptome of the C.elegans nervous system.

Profiling the transcriptomes of single cells without sacrificing spatial information is a major goal of the field of spatial transcriptomics, but current technologies require tradeoffs between single-cell resolution and whole-transcriptome coverage. In one animal species, the nematode worm Caenorhabditis elegans, a comprehensive spatial transcriptome with single-cell resolution is attainable using existing datasets, thanks to the worm's invariant cell lineage and a series of recently generated single cell transcriptomes. Here we present VISTA, which leverages these datasets to provide a visualization of the worm spatial transcriptome, focusing specifically on the nervous system. VISTA allows users to input a query gene and visualize its expression across all neurons in the form of a "spatial heatmap" in which the color of a cell reports the expression level. Underlying gene expression values (in Transcripts Per Million) are displayed when an individual cell is selected. We provide examples of the utility of VISTA for identifying striking new gene expression patterns in specific neurons, and for resolving cellular identities of ambiguous expression patterns generated from in vivo reporter genes. The ability to easily obtain gene-level snapshots of the neuronal spatial transcriptome should facilitate studies on neuron-specific gene expression and regulation and provide a template for the high-resolution spatial transcriptomes the field hopes to obtain for various animal species in the future. VISTA is freely available at the following URL: https://public.tableau.com/app/profile/smu.oit.data.insights/viz/VISTA_16814210566130/VISTA.

Effect of foot strike patterns and cutting angles on knee kinematics and kinetics during side-cutting maneuvers

Purpose Cutting maneuvers are important actions in multidirectional sports but associated with noncontact anterior cruciate ligament (ACL) injuries. This study aimed to investigate the effect of different foot strike patterns and cutting angles on knee kinematics and kinetics. Methods Twenty healthy male team sports athletes performed cuts with maximum speed at three angles (45°, 90°, and 135°) with different foot strike patterns (rearfoot strike [RFS] and forefoot strike [FFS]). A three-dimensional motion capture system combined with a force plate was used to collect makers trajectory and ground reaction force (GRF). Vertical GRF, and knee joint angles and moments were compared among these cutting tasks. Results Regardless of foot strike patterns, increased knee flexion angle, knee valgus moment, and knee internal rotation moment were observed during cutting to sharper angles (p &lt; 0.001). At 90° and 135°, the FFS condition remained in a varus position and showed lower knee flexion moment than the RFS condition (p ≤ 0.004). However, no significant differences in knee kinematic and kinetic variables were found between foot strike patterns during cutting to 45°. Conclusions These findings suggest that sharper cutting angles potentially increase the risk of ACL injury. Compared with the RFS pattern, the FFS pattern induces a slight knee varus angle and a lower knee flexion moment at sharper angles, which might further reduce the load placed on the knee.

Influence of Foot Strike Patterns on the Measurement of Leg Stiffness

Influence of Foot Strike Patterns on the Measurement of Leg Stiffness

Transitioning to Barefoot Running Using a Minimalist Shoe Intermediary: A Prospective Cohort Study.

The objectives of this study are, first, to investigate the probability of runners successfully transitioning from running in a traditional shoe to barefoot. Second, to identify prognostic indicators of failure of transition to barefoot running. Over 20 wk, 76 healthy runners (female, 40; age, 35.04 yr [SD, 8.9 yr]; body weight, 69.9 kg [SD 13.4 kg]) attempted to transition from running in traditional shoes to running barefoot. A minimalist shoe was used as an intermediary. Participants ran for 4 wk exclusively in provided traditional shoes followed by 4 wk of transitioning to minimalist shoes. This process was repeated to transition to barefoot running. Participants were followed up until they withdrew from the study or successfully transitioned to running barefoot. A survival analysis examined the weeks of successful transition. Along with sex and age, baseline measures of traditional shoe overall comfort, footstrike pattern, midfoot width mobility and plantar foot pressure pain threshold were examined as prognostic variables for failure to transition using Cox regression. The cumulative probability of successful transition to running barefoot was 70.8% (95% confidence interval [CI], 61%-83%). The primary footwear-related reason for withdrawal was pain, primarily in the foot ( n = 7), two runners had confirmed injuries. Runners exhibiting a rearfoot strike pattern and higher midfoot width mobility were more likely to fail to transition (hazard ratios [HR], 4.02; 95% CI, 1.33-12.16 and HR, 1.22; 95% CI, 1.05-1.42). Most runners who wish to run barefoot will be able to transition. Our study indicates that there may be biomechanical and anatomical characteristics that are prognostic of failing to transition when using a 20-wk transition period and an intermediary minimalist shoe. Whether a different transition process increases the probability of a success remains to be seen.

The maximum extent of the filaments and sheets in the cosmic web: an analysis of the SDSS DR17

ABSTRACT Filaments and sheets are striking visual patterns in cosmic web. The maximum extent of these large-scale structures are difficult to determine due to their structural variety and complexity. We construct a volume-limited sample of galaxies in a cubic region from the SDSS, divide it into smaller subcubes and shuffle them around. We quantify the average filamentarity and planarity in the 3D galaxy distribution as a function of the density threshold and compare them with those from the shuffled realizations of the original data. The analysis is repeated for different shuffling lengths by varying the size of the subcubes. The average filamentarity and planarity in the shuffled data show a significant reduction when the shuffling scales are smaller than the maximum size of the genuine filaments and sheets. We observe a statistically significant reduction in these statistical measures even at a shuffling scale of $\sim 130 \, {{\, \rm Mpc}}$, indicating that the filaments and sheets in three dimensions can extend up to this length scale. They may extend to somewhat larger length scales that are missed by our analysis due to the limited size of the SDSS data cube. We expect to determine these length scales by applying this method to deeper and larger surveys in future.

Biomechanical Analysis of Foot–Ankle Complex during Jogging with Rearfoot Strike versus Forefoot Strike

Background and Aim. In order to reduce foot and ankle injuries induced by jogging, two-foot strike patterns, rearfoot strike (RFS), and forefoot strike (FFS), were adopted and compared. First, RFS jogging and FFS jogging were experimentally studied, so as to acquire kinematic and kinetic data, including foot strike angle, knee flexion angle, and ground reaction force (GRF). Then, a 3D finite element model of foot–ankle complex was reconstructed from the scanned 2D-stacked images. Biomechanical characteristics, including plantar pressure, stress of metatarsals, midfoot bone, calcaneus and cartilage, and tensile force of plantar fascia and ligaments, were obtained. The results showed that RFS jogging and FFS jogging had a similar change trend and a close peak value of GRF. Since possessing more momentum in the push stage and less momentum in the brake stage, FFS jogging could be in favor of a higher jogging speed. However, FFS jogging produced larger metatarsal stress in the 5th metatarsal and much larger tensile force of plantar fascia, which might cause metatarsal fracture and heel pain. While RFS jogging produced larger plantar pressure in the hindfoot area, larger calcaneus stress, and much larger tarsal navicular stress, which might cause heel tissue injury, calcaneus damage, and stress fracture of naviculocuneiform joint. In addition, talocrural and talocalcaneal joint cartilage could bear jogging loads, as the peak contact pressure were both small in RFS jogging and FFS jogging. Therefore, jogging with rearfoot or FFS pattern should be chosen according to the health condition of foot–ankle parts.

Patterns of abundance, chromosomal localization, and domain organization among c-di-GMP-metabolizing genes revealed by comparative genomics of five alphaproteobacterial orders

BackgroundBis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) is a bacterial second messenger that affects diverse processes in different bacteria, including the cell cycle, motility, and biofilm formation. Its cellular levels are controlled by the opposing activities of two types of enzymes, with synthesis by diguanylate cyclases containing a GGDEF domain and degradation by phosphodiesterases containing either an HD-GYP or an EAL domain. These enzymes are ubiquitous in bacteria with up to 50 encoded in some genomes, the specific functions of which are mostly unknown.ResultsWe used comparative analyses to identify genomic patterns among genes encoding proteins with GGDEF, EAL, and HD-GYP domains in five orders of the class Alphaproteobacteria. GGDEF-containing sequences and GGDEF-EAL hybrids were the most abundant and had the highest diversity of co-occurring auxiliary domains while EAL and HD-GYP containing sequences were less abundant and less diverse with respect to auxiliary domains. There were striking patterns in the chromosomal localizations of the genes found in two of the orders. The Rhodobacterales’ EAL-encoding genes and Rhizobiales’ GGDEF-EAL-encoding genes showed opposing patterns of distribution compared to the GGDEF-encoding genes. In the Rhodobacterales, the GGDEF-encoding genes showed a tri-modal distribution with peaks mid-way between the origin (ori) and terminus (ter) of replication and at ter while the EAL-encoding genes peaked near ori. The patterns were more complex in the Rhizobiales, but the GGDEF-encoding genes were biased for localization near ter.ConclusionsThe observed patterns in the chromosomal localizations of these genes suggest a coupling of synthesis and hydrolysis of c-di-GMP with the cell cycle. Moreover, the higher proportions and diversities of auxiliary domains associated with GGDEF domains and GGDEF-EAL hybrids compared to EAL or HD-GYP domains could indicate that more stimuli affect synthesis compared to hydrolysis of c-di-GMP.

Validity and reliability of inertial measurement units measurements for running kinematics in different foot strike pattern runners.

This study aimed to assess the validity and reliability of the three-dimensional joint kinematic outcomes obtained by the inertial measurement units (IMUs) for runners with rearfoot strike pattern (RFS) and non-rearfoot strike pattern (NRFS). The IMUs system and optical motion capture system were used to simultaneous collect 3D kinematic of lower extremity joint data from participants running at 12km/h. The joint angle waveforms showed a high correlation between the two systems after the offset correction in the sagittal plane (NRFS: coefficient of multiple correlation (CMC) = 0.924-0.968, root mean square error (RMSE) = 4.6°-13.7°; RFS: CMC = 0.930-0.965, RMSE = 3.1°-7.7°), but revealed high variability in the frontal and transverse planes (NRFS: CMC = 0.924-0.968, RMSE = 4.6°-13.7°; RFS: CMC = 0.930-0.965, RMSE = 3.1°-7.7°). The between-rater and between-day reliability were shown to be very good to excellent in the sagittal plane (between-rater: NRFS: CMC = 0.967-0.975, RMSE = 1.9°-2.9°, RFS: CMC = 0.922-0.989, RMSE = 1.0°-2.5°; between-day: NRFS: CMC = 0.950-0.978, RMSE = 1.6°-2.7°, RFS: CMC = 0.920-0.989, RMSE = 1.7°-2.2°), whereas the reliability was weak to very good (between-rater: NRFS: CMC = 0.480-0.947, RMSE = 1.1°-2.7°, RFS: CMC = 0.646-0.873, RMSE = 0.7°-2.4°; between-day: NRFS: CMC = 0.666-0.867, RMSE = 0.7°-2.8°, RFS: CMC = 0.321-0.805, RMSE = 0.9°-5.0°) in the frontal and transverse planes across all joints in both types of runners. The IMUs system was a feasible tool for measuring lower extremity joint kinematics in the sagittal plane during running, especially for RFS runners. However, the joint kinematics data in frontal and transverse planes derived by the IMUs system need to be used with caution.

Abstract IA007: Transforming chromatin: Oncohistone mutations in pediatric high-grade glioma

Abstract Pediatric diffuse high-grade gliomas (HGGs) are a heterogeneous spectrum of disease with abysmal survival rates. Approximately half of diffuse high-grade gliomas in children arise in midline structures predominantly the brainstem, but also thalamus, cerebellum and spinal cord. Approximately 80% of these tumors harbor H3 K27M mutations, which result in dramatic depletion of the post-translational modification H3K27me3. Alternative mutations in diffuse midline gliomas can result in similar reduction of H3K27me3, leading to a redefined classification of this collection of tumors as diffuse midline glioma, H3 K27-altered. In contrast, distinct histone H3 mutations, H3.3 G34R/V, are found in approximately 30% of diffuse gliomas arising in the cerebral hemispheres of older adolescents and young adults, defining the tumor subgroup of diffuse hemispheric glioma, H3 G34-mutant. The striking spatiotemporal pattern of these histone mutations, termed oncohistones, indicates an intimate association between epigenetic dysregulation, brain development, and tumorigenesis. We will discuss use of genetically engineered and patient-derived models to investigate the contribution of oncohistone mutations to disrupted development, epigenetic dysregulation and gliomagenesis. Citation Format: Kaitlin Budd, Chang-Hyuk Kwon, Lawryn H. Kasper, Christopher Roberts, Jordan Roach, Jennifer Ocasio-Adorno, Jon D. Larson, Suzanne J. Baker. Transforming chromatin: Oncohistone mutations in pediatric high-grade glioma. [abstract]. In: Proceedings of the AACR Special Conference: Cancer Epigenomics; 2022 Oct 6-8; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2022;82(23 Suppl_2):Abstract nr IA007.

Changes of the in vivo kinematics of the human medial longitudinal foot arch, first metatarsophalangeal joint, and the length of plantar fascia in different running patterns.

Accurately obtaining the in vivo motion of the medial longitudinal arch (MLA), first metatarsophalangeal joint (MTPJ), and plantar fascia (PF) is essential for analyzing the biomechanics of these structures in different running strike patterns. Most previous studies on the biomechanics of the MLA, first MTPJ, and PF have been based on traditional skin-marker-based motion capture, which cannot acquire the natural foot motion. Therefore, this study aimed to 1) describe the movement of the MLA, first MTPJ, and PF during running by using the high-speed dual fluoroscopic imaging system (DFIS) and 2) explore changes of the in vivo kinematics of the MLA and first MTPJ, and the length of the PF during the stance phase of running with different foot strike patterns. Fifteen healthy male runners all of whom ran with a regular rearfoot strike (RFS) pattern were required to run with forefoot strike (FFS) and RFS patterns. Computed tomography scans were taken from each participant's right foot for the construction of 3D models (the calcaneus, first metatarsal, and first proximal phalanges) and local coordinate systems. A high-speed DFIS (100Hz) and 3D force platform (2,000Hz) were used to acquire X-ray images of the foot bones and ground reaction force data during the stance phase of running (3m/s ± 5%) simultaneously. Then, 3D-2D registration was used to obtain the in vivo kinematic data of the MLA and first MTPJ and the length of the PF. When compared with RFS, in FFS, 1) the range of motion (ROM) of the medial/lateral (5.84 ± 5.61mm vs. 0.75 ± 3.38mm, p = 0.002), anterior/posterior (14.64 ± 4.33mm vs. 11.18 ± 3.56 mm, p = 0.010), plantarflexion/dorsiflexion (7.13 ± 3.22° vs. 1.63 ± 3.29°, p < 0.001), and adduction/abduction (-3.89 ± 3.85° vs. -0.64 ± 4.39°, p = 0.034) motions of the MLA were increased significantly; 2) the ROM of the anterior/posterior (7.81 ± 2.84mm vs. 6.24 ± 3.43 mm, p = 0.003), superior/inferior (2.11 ± 2.06mm vs. -0.57 ± 1.65 mm, p = 0.001), and extension/flexion (-9.68 ± 9.16° vs. -5.72 ± 7.33°, p = 0.018) motions of the first MTPJ were increased significantly; 3) the maximum strain (0.093 ± 0.023 vs. 0.075 ± 0.020, p < 0.001) and the maximum power (4.36 ± 1.51W/kg vs. 3.06 ± 1.39W/kg, p < 0.001) of the PF were increased significantly. Running with FFS may increase deformation, energy storage, and release of the MLA and PF, as well as the push-off effect of the MTPJ. Meanwhile, the maximum extension angle of the first MTPJ and MLA deformation increased in FFS, which showed that the PF experienced more stretch and potentially indicated that FFS enhanced the PF mechanical responses.

Contrasting continental patterns of adaptive population divergence in the holarctic ectomycorrhizal fungus Boletus edulis.

In the hyperdiverse fungi, the process of speciation is virtually unknown, including for the > 20 000 species of ectomycorrhizal mutualists. To understand this process, we investigated patterns of genome-wide differentiation in the ectomycorrhizal porcini mushroom, Boletus edulis, a globally distributed species complex with broad ecological amplitude. By whole-genome sequencing 160 individuals from across the Northern Hemisphere, we genotyped 792 923 single nucleotide polymorphismsto characterize patterns of genome-wide differentiation and to identify the adaptive processes shaping global population structure. We show that B. edulis exhibits contrasting patterns of genomic divergence between continents, with multiple lineages present across North America, while a single lineage dominates Europe. These geographical lineages are inferred to have diverged 1.62-2.66 million years ago, during a period of climatic upheaval and the onset of glaciation in the Pliocene-Pleistocene boundary. High levels of genomic differentiation were observed among lineages despite evidence of substantial and ongoing introgression. Genome scans, demographic inference, and ecological niche models suggest that genomic differentiation is maintained by environmental adaptation, not physical isolation. Our study uncovers striking patterns of genome-wide differentiation on a global scale and emphasizes the importance of local adaptation and ecologically mediated divergence, rather than prezygotic barriers such as allopatry or genomic incompatibility, in fungal population differentiation.