Striga Hermonthica Research Articles

Rational design of Striga hermonthica-specific seed germination inhibitors.

The obligate hemiparasitic weed Striga hermonthica grows on cereal roots and presents a severe threat to global food security by causing enormous yield losses, particularly in sub-Saharan Africa. The rapidly increasing Striga seed bank in infested soils provides a major obstacle in controlling this weed. Striga seeds require host-derived strigolactones (SLs) for germination, and corresponding antagonists could be used as germination inhibitors. Recently, we demonstrated that the common detergent Triton X-100 is a specific inhibitor of Striga seed germination by binding noncovalently to its receptor, S. hermonthica HYPO-SENSITIVE TO LIGHT 7 (ShHTL7), without blocking the rice (Oryza sativa) SL receptor DWARF14 (OsD14). Moreover, triazole ureas, the potent covalently binding antagonists of rice SL perception with much higher activity toward OsD14, showed inhibition of Striga but were less specific. Considering that Triton X-100 is not suitable for field application and by combining structural elements of Triton and triazole urea, we developed two hybrid compounds, KK023-N1 and KK023-N2, as potential Striga-specific germination inhibitors. Both compounds blocked the hydrolysis activity of ShHTL7 but did not affect that of OsD14. Binding of KK023-N1 diminished ShHTL7 interaction with S. hermonthica MORE AXILLARY BRANCHING 2, a major component in SL signal transduction, and increased ShHTL7 thermal specificity. Docking studies indicate that KK023-N1 binding is not covalent but is caused by hydrophobic interactions. Finally, in vitro and greenhouse tests revealed specific inhibition of Striga seed germination, which led to a 38% reduction in Striga infestation in pot experiments. These findings reveal that KK023-N1 is a potential candidate for combating Striga and a promising basis for rational design and development of further Striga-specific herbicides.

Farmer’s Perception and Bio-Indicators of Soil Fertility in Eastern Niger

Since ancient times, peasants have relied on the presence of some plants or animals to assess soi’s fertility. This study carried out in Dogo site in Niger and that aim is ti determine the main farmer indicators of soil fertility assessment. For this, an individual semi-structured questionnaire was administered to the farmers. Results showed that several types of biological indicators are used by farmers to assess soil’s fertility through the presence or absence of some plants and/or animals. Thus, Cenchrus biflorus, Cyperus alopeuroides, Alysicarpus ovalifolius, Brachiaria spp have been cited as non-woody fertility indicator species. As for Faidherbia albida, Acacia nilotica, Piliostigma reticulatum, they were cited as woody species indicative of fertile soils. Also, Striga hermonthica, Aristida stipoides, Ipomoea koschyana, Euphorbia forskalii, Ipomoea cosunosperma, Alysicarpus ovalifolius, Commelina benghalensis were considered as non-woody indicator species of poor soils and Prosopis juliflora, Lannasinarima indicuticosa in poor soils woody species.

Mycotoxins produced by Fusarium spp. isolated from Striga hermonthica (Del.) Benth. in Sudan

isolates of F. solani and one isolate of each of F. oxysporum, F. nygamai, F. equiseti, and F. compactum were fully toxicologically characterized. These Fusaria were isolated from naturally infected Striga hermonthica plants collected from sorghum cultivations in central (Gezira), eastern (Gadarif), and southeastern Sudan (Damazin). HPLC/MS or HPLC/DAD system was used for the toxicological characterization of Fusarium spp. using nivalenol, deoxynivalenol, 3-acetyl deoxynivalenol, 15-Acetyl deoxynivalenol, fusarenon X, T2-toxin, HT-2 toxin, diacetoxyscirpenol, zearalenone, fumonisin B (B1 and B2), enniatin B, B1 and A and fusaric acid as toxin standards. The three isolates of F. solani produced none of the above toxins. Fusarium oxysporum and F. nygamai produced fusaric acid. Fusarium nygamai also produced fumonisns B1, B2, and B3. Fusarium equiseti produced nivalenol, fusarenon X, and diacetoxyscirpenol. Fusarium compactum produced nivalenol. This is the first report of nivalenol production by a strain of F. compactum. Non toxin-production by the three isolates of F. solani and production of only fusaric acid by F. oxysporum make them toxicologically safe for further evaluations as biocontrol agents for the witchweed.

Biocontrol of Striga hermonthica (Del.)Benth. in Sorghum

Twenty fungal isolates were collected from diseased Striga plants at the Faculty of Agriculture Demonstration Farm , University of Khartoum Farm and the Gezira Scheme .Eighteen isolates were identified as Aspergillus niger while only two of the isolates were identified as A. flavus .Pathogenicity tests of these fungi on Striga hermonthica in vitro and in the glasshouse revealed the potential to utilize Aspergillus spp. in Striga hermonthica biocontrol

English

Sorghum is an important staple food crop in Kenya, but its yield is low due to many constraints which include diseases, drought, soil fertility, and pests, notably parasitic weed Striga hermonthica. Twenty elite sorghum genotypes and one resistant check were evaluated in three replications in a complete randomized design in Agar Gel (AG) experiment and randomized complete block design on selected Striga hotspot farms at Kadel and Nyahera in western Kenya. Significant differences (p<0.001) were realized both in the fields (plant height, dry shoot biomass yield, grain yield, field Striga count, and Striga damage ratings) and in the AG experiment with respect to maximum germination distance and numbers of induced germinated Striga seeds. Among the sorghum genotypes evaluated under field conditions, T53B, N57, N68, C26, IESV 92036-SH, T30B, and Uyoma 47 White genotypes had stable yields under Striga infestation in the two sites, whereas Nyadundo 1, Nyadundo 2, and Uyoma 8 were susceptible. In the AG experiment, E117B, T30B, Uyoma 8, Uyoma 42 STR, and T53B induced high numbers of germinated Striga seeds with maximum germination distances (MGD). In contrast, Uyoma 47 Brown, IESV 92038/2-SH, and IESV 92036-SH sorghum genotypes secreted low amounts of SLS suggesting resistance. This study has identified and selected four Striga tolerant (T53B, N68, N57, and T 30B) and resistant (C 26, Uyoma 47 Brown, IESV 92038/2-SH, and IESV 92036-SH) sorghum genotypes which can be utilized in further research programs or adopted to improve sorghum productivity in the highly Striga infested regions of western Kenya. Key words: Striga, sorghum genotypes, resistant, susceptible, tolerant, Agar Gel experiment

Role of substrate recognition in modulating strigolactone receptor selectivity in witchweed

Witchweed, or Striga hermonthica, is a parasitic weed that destroys billions of dollars' worth of crops globally every year. Its germination is stimulated by strigolactones exuded by its host plants. Despite high sequence, structure, and ligand-binding site conservation across different plant species, one strigolactone receptor in witchweed, ShHTL7, uniquely exhibits a picomolar EC50 for downstream signaling. Previous biochemical and structural analyses have hypothesized that this unique ligand sensitivity can be attributed to a large binding pocket volume in ShHTL7 resulting in enhanced ability to bind substrates, but additional structural details of the substrate-binding process would help explain its role in modulating the ligand selectivity. Using long-timescale molecular dynamics simulations, we demonstrate that mutations at the entrance of the binding pocket facilitate a more direct ligand-binding pathway to ShHTL7, whereas hydrophobicity at the binding pocket entrance results in a stable “anchored” state. We also demonstrate that several residues on the D-loop of AtD14 stabilize catalytically inactive conformations. Finally, we show that strigolactone selectivity is not modulated by binding pocket volume. Our results indicate that while ligand binding is not the sole modulator of strigolactone receptor selectivity, it is a significant contributing factor. These results can be used to inform the design of selective antagonists for strigolactone receptors in witchweed.

Two novel poty-like viruses identified from the transcriptome data of purple witchweed (Striga hermonthica).

Potyvirids (the family Potyviridae) are the largest family of plant RNA viruses. Two novel potyvirid viruses, Striga-associated poty-like virus 1 (SaPlV1) and Striga-associated poty-like virus 2 (SaPlV2), were identified from the transcriptome data of purple witchweed (Striga hermonthica). SaPlV1 was most closely related to bellflower veinal mottle virus (BVMoV), the only member of the genus Bevemovirus, and then to macluraviruses (the genus Macluravirus). The SaPlV1 genome encodes a 2462-amino acid (aa) polyprotein that may be cleaved into nine mature peptides. The cleavage sites of SaPlV1, BVMoV, and macluravirus polyproteins shared strong sequence similarities. SaPlV2 was most closely related to celery latent virus, the sole species of the genus Celavirus, which is the most divergent potyvirid genus. The SaPlV2 polyprotein contained 3329 aa and it may be cleaved into at least seven or eight mature peptides. Phylogenetic analysis suggested that SaPlV1 and SaPlV2 may be novel species of the genera Bevemovirus and Celavirus, respectively. The genome sequences of SaPlV1 and SaPlV2 are useful resources for studying the genome evolution of potyvirids. Keywords: Striga-associated poty-like virus 1; Striga-associated poty-like virus 2; Potyviridae; Beve- movirus; Celavirus; purple witchweed; Striga hermonthica.

Characterization of growth and development of sorghum genotypes with differential susceptibility to Striga hermonthica.

Two sorghum varieties, Shanqui Red (SQR) and SRN39, have distinct levels of susceptibility to the parasitic weed Striga hermonthica, which have been attributed to different strigolactone composition within their root exudates. Root exudates of the Striga-susceptible variety Shanqui Red (SQR) contain primarily 5-deoxystrigol, which has a high efficiency for inducing Striga germination. SRN39 roots primarily exude orobanchol, leading to reduced Striga germination and making this variety resistant to Striga. The structural diversity in exuded strigolactones is determined by a polymorphism in the LOW GERMINATION STIMULANT 1 (LGS1) locus. Yet, the genetic diversity between SQR and SRN39 is broad and has not been addressed in terms of growth and development. Here, we demonstrate additional differences between SQR and SRN39 by phenotypic and molecular characterization. A suite of genes related to metabolism was differentially expressed between SQR and SRN39. Increased levels of gibberellin precursors in SRN39 were accompanied by slower growth rate and developmental delay and we observed an overall increased SRN39 biomass. The slow-down in growth and differences in transcriptome profiles of SRN39 were strongly associated with plant age. Additionally, enhanced lateral root growth was observed in SRN39 and three additional genotypes exuding primarily orobanchol. In summary, we demonstrate that the differences between SQR and SRN39 reach further than the changes in strigolactone profile in the root exudate and translate into alterations in growth and development.

The Phtheirospermum japonicum isopentenyltransferase PjIPT1a regulates host cytokinin responses in Arabidopsis.

The hemiparasitic plant Phtheirospermum japonicum (Phtheirospermum) is a nutritional specialist that supplements its nutrient requirements by parasitizing other plants through haustoria. During parasitism, the Phtheirospermum haustorium transfers hypertrophy-inducing cytokinins (CKs) to the infected host root. The CK biosynthesis genes required for haustorium-derived CKs and the induction of hypertrophy are still unknown. We searched for haustorium-expressed isopentenyltransferases (IPTs) that catalyze the first step of CK biosynthesis, confirmed the specific expression by invivo imaging of a promoter-reporter, and further analyzed the subcellular localization, the enzymatic function and contribution to inducing hypertrophy by studying CRISPR-Cas9-induced Phtheirospermum mutants. PjIPT1a was expressed in intrusive cells of the haustorium close to the host vasculature. PjIPT1a and its closest homolog PjIPT1b located to the cytosol and showed IPT activity invitro with differences in substrate specificity. Mutating PjIPT1a abolished parasite-induced CK responses in the host. A homolog of PjIPT1a also was identified in the related weed Striga hermonthica. With PjIPT1a, we identified the IPT enzyme that induces CK responses in Phtheirospermum japonicum-infected Arabidopsis roots. We propose that PjIPT1a exemplifies how parasitism-related functions evolve through gene duplications and neofunctionalization.

Production Potential of Allelopathic Rice, Cymbopogon, Desmodium, Mucuna and Maize

Allelochemicals regulate the productivity of crop ecosystems. A screen house experiment was conducted (2016) at the National Crops Resources Research Institute, Namulonge, Uganda to determine the effects of NERICA 1 rice (an interspecific hybrid between Oryza sativa and O. glaberrima species), Cymbopogon nardus (C), Desmodium uncinatum (D), Mucuna pruriens (Mc) and LONGE 6H, Zea mays (Mz) on crop relative growth rates (RGR), nitrogen (N), phosphorus (P) and potassium (K) nutrient levels. One field study was conducted on a farm (2017) to establish the allelopathic interactive effects of RCDMcMz on Striga hermonthica (a parasitic weed), crop competition and productivity. Data was collected on striga, RMz growth, nutrient levels and yield. Potted rice reduced (30%-47%) in root length but Mz leaf length increased (31% & 15%) with Mc & D. RMc reduced (73%) striga and increased rice RGR (14-42 days). RD similarly reduced (67%) striga. RC increased (96%, 44% & 73%) rice NPK uptake, RGR (14-42 days), reduced (57%) striga and increased (1.56) the combined land equivalent ratio (CLER) and rice grain yields. RMz reduced (16%, 38% & 38%) rice NPK reserves, RGR (14-42 days), CLER (1.0), grain yields and increased (36%) striga. RD recorded higher CLER (1.56). MzMc reduced (15% & 27%) maize P uptake and NP uptake increased (42% & 9.3%) under MzC & MzD (73% & 29%). RMc increased rice RGR (14-42 days). Maize RGR (14-28 days) increased under MzD, MzMc & MzC and reduced (28-42 days) under MzD, RC & MzMc.. The ecosystems’ productivity was attributed to allelopathy.

Agronomic Performance Of S1 Maize Lines Derived From A Bi-Parental Cross Under Infested And Striga Free Environments

Striga hermonthica, causes up to 100% yield loss in maize production in sub-Saharan Africa. Developing Striga resistant maize cultivars could be a major component of integrated Striga management strategies. This study aims at assessing the agronomic performance of S1 breeding lines in improving maize for Striga resistance. Two hundred S1 lines have been evaluated under artificial infestation Striga and Striga-free conditions in Benin for two years during 2018 and 2019 growing seasons using alpha-lattice design (51 x 4) with two replicates. Twelve agro-morphological and Striga adaptive traits have been assessed. The tested lines have displayed high genetic variability for most agronomic and Striga adaptive traits. The S1 lines exhibited high grain yield than their parents with averages of 2,552.72±593 kg ha-1 and 2,965.67±635.86 kg ha-1 under Striga artificial infestation and Striga-free conditions, respectively. Grain yield has displayed high positive and significant genetic and phenotypic correlations with ears per plant and high negative correlations with days to 50% silking, ears aspect, and Striga damage rating at 8 and 10 weeks after planting (WAP). Useful traits like ears per plant, days to 50% silking, ears aspect, number of emerged Striga plants and Striga rating at 10 WAP could assist for indirect selection under Striga conditions. Based on the selection index, a total of 15 S1 lines have been identified as top ranking and can be used as sources of resistance or tolerance genes to Striga and further improvement in maize breeding in future.

Genetic Characterization Of Early Generation Lines Using SNPS Makers And Agronomic Traits For Resistance To Striga Improvement In Maize

The characterization of a greater number of lines hence potentially increase the efficiency of maize breeding programs. This study aims to assess the genetic variation and relationships existing within a population of 177 lines and the two parental lines, using 8,883 SNPs markers obtained from sequencing genotyping (GBS) and four agronomic traits. Two hundred S 1 lines and four checks including the two parents have been evaluated under Striga hermonthica infestation in Benin Republic and Nigeria for two years during 2018 and 2019 growing seasons using 51 x 4 lattice design with two replicates. The UPGMA phylogeny, was used to group the progenies based on their genetic similarity. The tested lines have displayed high genetic variability for all the agronomic traits. Analysis molecular revealed that the polymorphism information content has been varied from 0.047 to 0.50, with average of 0.37, and 63% of the SNP makers were highly polymorphic. The population has displayed a moderate diversity with average genetic diversity of 0.44. The estimated genetic distance has been varied from 0.01 to 0.79 and the highest distance has been observed between the two parental lines. UPGMA clustering based on the Gower dissimilarity matrix grouped the 177 lines into two clusters (I and II) at 30% genetic similarity threshold. The estimated genetic distances between lines showed that all the progenies were genetically related to the two parental lines; and have the potential to provide new favorable alleles for the development of high-performing, Striga-resistant and/or Striga-tolerant maize populations.

Assessing performance of white endosperm testers with varying resistance reactions to Striga ( Striga hermonthica ) for evaluating resistant maize ( Zea mays ) inbred lines

Assessing performance of white endosperm testers with varying resistance reactions to Striga ( Striga hermonthica ) for evaluating resistant maize ( Zea mays ) inbred lines

Genotypic Variation in Cultivated and Wild Sorghum Genotypes in Response to Striga hermonthica Infestation.

Striga hermonthica is the most important parasitic weed in sub-Saharan Africa and remains one of the most devastating biotic factors affecting sorghum production in the western regions of Kenya. Farmers have traditionally managed Striga using cultural methods, but the most effective and practical solution to poor smallholder farmers is to develop Striga-resistant varieties. This study was undertaken with the aim of identifying new sources of resistance to Striga in comparison with the conventional sources as standard checks. We evaluated 64 sorghum genotypes consisting of wild relatives, landraces, improved varieties, and fourth filial generation (F4) progenies in both a field trial and a pot trial. Data were collected for days to 50% flowering (DTF), dry panicle weight (DPW, g), plant height (PH, cm), yield (YLD, t ha−1), 100-grain weight (HGW, g), overall disease score (ODS), overall pest score (OPS), area under Striga number progress curve (ASNPC), maximum above-ground Striga (NSmax), and number of Striga-forming capsules (NSFC) at relevant stages. Genetic diversity and hybridity confirmation was determined using Diversity Arrays Technology sequencing (DArT-seq). Residual heterosis for HGW and NSmax was calculated as the percent increase or decrease in performance of F4 crossover midparent (MP). The top 10 best yielding genotypes were predominantly F4 crosses in both experiments, all of which yielded better than resistant checks, except FRAMIDA in the field trial and HAKIKA in the pot trial. Five F4 progenies (ICSVIII IN × E36-1, LANDIWHITE × B35, B35 × E36-1, F6YQ212 × B35, and ICSVIII IN × LODOKA) recorded some of the highest HGW in both trials revealing their stability in good performance. Three genotypes (F6YQ212, GBK045827, and F6YQ212xB35) and one check (SRN39) were among the most resistant to Striga in both trials. SNPs generated from DArT-seq grouped the genotypes into three major clusters, with all resistant checks grouping in the same cluster except N13. We identified more resistant and high-yielding genotypes than the conventional checks, especially among the F4 crosses, which should be promoted for adoption by farmers. Future studies will need to look for more diverse sources of Striga resistance and pyramid different mechanisms of resistance into farmer-preferred varieties to enhance the durability of Striga resistance in the fields of farmers.

Germplasm Bred for Resistance to Striga hermonthica Exhibited High Resistance Levels to Striga asiatica Compared to Commercial Checks

Parasitic weeds belonging to the Orobanchaceae family are a menace in Sub-Saharan African (SSA). Specifically, the two witchweeds from the genus Striga, S. hermonthica and S. asiatica, are jointly responsible for land abandonments and cereal yield reductions in the SSA. Factorial experiments involving fourteen maize genotypes and two levels of Striga asiatica infestation (infested and noninfested) were conducted under pot and laboratory experiments at the Department of Plant Production Sciences and Technologies, University of Zimbabwe, during the 2014/2015 season. A 14 × 2 factorial pot experiment was arranged in a 7 × 4 α-lattice design replicated four times, whereas the laboratory agar gel was arranged in a complete randomized design with four replications. Results revealed significant differences ( p < 0.05) for S. asiatica and genotype main effects for the recorded traits. S. asiatica-infested genotypes had lower biomasses than noninfested ones. However, the University of Zimbabwe bred hybrids such as Ax31, Ax28, Ax7, and Ax32 had similar plant heights and stem, leaf, and cob biomass across the two Striga levels unlike the local checks, particularly SC513, SC537, and SC637, under Striga infestation. Moreover, these genotypes also had the least Striga germination percentage and furthest germination distance indicating that they are resistant/tolerant to Striga asiatica. The University of Zimbabwe bred hybrids, using Striga hermonthica resistance from the International Institute of Tropical Agriculture, exhibited resistance/tolerance to Striga asiatica compared to local checks. Therefore, these varieties could offer a better and viable Striga spp. control option to farmers in both S. hermonthica and S. asiatica endemic areas.

Nitrogen fertilizer and cattle manure for Striga (Striga hermonthica) management and enhancement of sorghum productivity in northwest Ethiopia

Striga hermonthica is a major biotic threat to worldwide food security, causing tremendous losses in yields of the main staple crops including sorghum, in sub-Saharan Africa. Despite the numerous ways of Striga control available, sorghum production in North Gondar is below its potential. The concept of combining two or more control measures is the current focus. Nitrogen (0, 46, and 92-kilo grams h−1) and cattle manure (0, 10, and 20 tons h−1) application for Striga control in sorghum, was evaluated in 2017/2018, in northwest Ethiopia. Factorial combination in randomized complete block design with three replications and local sorghum ‘Deber’ a test crop was used. The interaction of (N 92 kilogram) and cattle manure (20 t h−1) gave the highest (1.23 t) grain and (13.49 t h−1) biomass with the least Striga counts (81.3 per plot). The lowest biomass, grain, and highest Striga count were in the control. Economic analysis results showed the highest and lowest net returns 24,643 and 7230 Ethiopian birr h−1, in N 92 kg and 20 t h−1 manure and the control, respectively. Therefore, Nitrogen and cattle manure is necessary to control Striga in sorghum and soil rehabilitation through the restoration of biomass productivity and depleting Striga seed bank in the soil. N 92 kg and cattle manure 20 t h−1 can be recommended for the study area.

Gene Action, Heterotic Patterns, and Inter-Trait Relationships of Early Maturing Pro-Vitamin A Maize Inbred Lines and Performance of Testcrosses under Contrasting Environments

Vitamin A deficiency is the leading cause of night blindness, total blindness, maternal and childhood mortality in developing countries. Drought, low soil nitrogen and Striga hermonthica parasitism are major constraints to maize production in sub-Saharan Africa (SSA). Thus, the development of multiple stress tolerant maize varieties with elevated levels of PVA is an economically feasible approach to simultaneously tackle malnutrition and food insecurity in SSA. The objectives of this study were to determine the gene action modulating the inheritance of grain yield and other traits, group the inbred lines, investigate inter-trait relationships among grain yield and other traits and assess the performance and stability of single-cross hybrids derived from a set of inbred lines under stress and non-stress environments. One hundred and ninety diallel crosses plus six hybrid checks were evaluated under managed drought at Ikenne during the 2016/17 and 2017/18 dry seasons, low soil N conditions at Mokwa and Ile-Ife, Striga infestation at Abuja and Mokwa, and optimal management conditions at Ikenne, Kadawa, Abuja, Bagauda and Mokwa during the 2016 and 2017 growing seasons. Both additive and non-additive gene actions were prominent in the inheritance of grain yield and other measured traits under stress and optimal management conditions. However, additive gene action was preponderant over the non-additive. The PVA inbreds were classified into three heterotic groups with TZEI 25 and TZEIOR 164 identified as inbred testers for heterotic groups 2 and 3, respectively. Plant and ear heights, ears per plant, plant and ear aspects were identified as reliable secondary traits for genetic enhancement of grain yield under both stress and non-stress conditions. Hybrids TZEIOR 4 × TZEIOR 158 and TZEIOR 119 × TZEIOR 158 were outstanding in performance and should be tested extensively for possible commercialization to combat malnutrition and food insecurity in SSA.

Ethnoveterinary knowledge of sheep and goat farmers in Benin (West Africa): effect of socioeconomic and environmental factors

Sheep and goats are two of the main animal species raised in Benin and one of the main sources of income for people living in rural areas. Faced with the inaccessibility of synthetic veterinary products and their low purchasing power, the majority of breeders use ethnoveterinary practices to treat small ruminants diseases. The specific objectives of the current study were (1) to document the traditional knowledge regarding the disorder groups treated and the medicinal plants used in the health and zootechnical management of small ruminants in Benin and (2) to assess the effect of gender, ethnicity, agro-ecological zone and herd size associated with them. To achieve these objectives, an ethnoveterinary survey was conducted in different agro-ecological zones from September 2018 to February 2019. A questionnaire was administered to 506 breeders. The data were analyzed through calculation of the Fidelity Level (FL), Cultural Importance Index (CI) and Informant Consensus Factor (ICF). Ten disorder groups were treated by the people surveyed. These were mainly digestive disorders (D) and reproductive disorders (W), both presenting a ICF value of 0.8. A total of 101 medicinal plants belonging to 42 families and 90 genera were recorded. Spondias mombin, Zanthoxylum zanthoxyloides and Khaya senegalensis were the most important plants with CI values of 0.208, 0.125 and 0.121, respectively. Gender, ethnicity, agro-ecological zone and flocks size were the socioeconomic and environmental factors that significantly influenced the level of ethnoveterinary knowledge. Chemical and biological analysis are needed on less studied plants such as, Striga hermonthica, Crossopteryx febrifuga, Elaeis guineensis and Momordica charantia.

Striga (Witchweed) Threats to Cereal Crops Production and Its Management: A Review

Striga (witchweed) is one of the most successful parasitic weeds of cereal crops in Africa. It is ‘a poor farmer’s problem’ as there is a near perfect ecological overlap between areas of Striga infestation and where hunger prevails. It is originated in Semien hills of Ethiopia and the Nubian hills of Sudan and later expanded in about 42 African countries. Striga hermonthica, Striga asiatica, Striga gesnerioides, and Striga aspera constitute the greatest economic threat to cereal crops’ yield losses. They are obligate root parasites causing growth inhibition and yield losses of 20-100% in maize, rice, sorghum, pearl millet, finger millet, sugar cane and cowpea. Cultural practices such as long-term rotational cultivation of cereal crops with legume crops unaffected by the parasite is effective in managing the weed. Besides, biological control by use of parasitic fungi ( Fusarium oxysporum and Vesicular arbuscular mycorrhiza) play a role in managing the weed. In addition, chemical control with imidazolinone herbicide, ethylene gas, dicamba and 2,4-D are effective in managing striga in cereal crops in extreme cases. Integrated use of striga resistant crop varieties with water conservation practices, soil fertility amendment and use of parasitic fungi ( Fusarium oxysporum and Vesicular arbuscular mycorrhiza) is effective in control, economically safe, socially acceptable and environmentally friendly than a single control measure. Therefore, the promising integrated striga management practices should be highly promoted. Moreover, host and Striga species specific integrated Striga management should be designed. Keywords: Haustoria; Obligate parasite; Root parasite; Strigolactones; Witchweed DOI: 10.7176/ALST/88-02 Publication date: July 31 st 2021

Development of Extra-Early Provitamin A Quality Protein Maize Inbreds with Resistance/Tolerance to Striga hermonthica and Soil Nitrogen Stress

A hemiparasitic plant, Striga hermonthica (Del.) Benth and soil nitrogen stress are the key constraints to maize (Zea mays L.) productivity in sub-Saharan Africa, where commonly cultivated maize is the normal endosperm type that is deficient in provitamin A, tryptophan and lysine (PVATL). Seventy-six extra-early maize inbreds with provitamin A, tryptophan, and lysine qualities (TZEEIORQ) were developed to address these constraints, and four checks were assessed under Striga, low and high nitrogen conditions at three locations in Nigeria. The inbreds were further genotyped with two beta-carotene hydroxylase 1 (crtRB1) markers, and their seeds were quantified for provitamin A content. Significant (P < 0.01) genetic variations were observed for grain yield and other agronomic attributes of the inbreds under varying environmental conditions. Levels of PVATL for the inbreds ranged from 2.21–10.95 µg g−1, 0.04–0.08%, and 0.19–0.39%, respectively. Beta-carotene marker, crtRB1-3′TE, was polymorphic and grouped the inbreds into two. The marker was effective in identifying inbreds with moderate provitamin A content. Inbreds TZEEIORQ 5, TZEEIORQ 52, and TZEEIORQ 55 exhibited resistance to Striga, tolerance to nitrogen stress with moderate levels of PVATL and could be invaluable sources of favorable alleles for breeding nutritionally improved maize varieties with resistance/tolerance to Striga and soil nitrogen stress.