Stress Conditions Research Articles

Genome-wide analysis of filamentous temperature-sensitive H protease (ftsH) gene family in soybean.

The filamentous temperature-sensitive H protease (ftsH) gene family belongs to the ATP-dependent zinc metalloproteins, and ftsH genes play critical roles in plant chloroplast development and photosynthesis. In this study, we performed genome-wide identification and a systematic analysis of soybean ftsH genes. A total of 18 GmftsH genes were identified. The subcellular localization was predicted to be mainly in cell membranes and chloroplasts, and the gene structures, conserved motifs, evolutionary relationships, and expression patterns were comprehensively analyzed. Phylogenetic analysis of the ftsH gene family from soybean and various other species revealed six distinct clades, all of which showed a close relationship to Arabidopsis thaliana. The members of the GmftsH gene family were distributed on 13 soybean chromosomes, with intron numbers ranging from 3 to 15, 13 pairs of repetitive segment. The covariance between these genes and related genes in different species of Oryza sativa, Zea mays, and Arabidopsis thaliana was further investigated. The transcript expression data revealed that the genes of this family showed different expression patterns in three parts, the root, stem, and leaf, and most of the genes were highly expressed in the leaves of the soybean plants. Fluorescence-based real-time quantitative PCR (qRT-PCR) showed that the expression level of GmftsH genes varied under different stress treatments. Specifically, the genes within this family exhibited various induction levels in response to stress conditions of 4℃, 20% PEG-6000, and 100 mmol/L NaCl. These findings suggest that the GmftsH gene family may play a crucial role in the abiotic stress response in soybeans. It was also found that the GmftsH7 gene was localized on the cell membrane, and its expression was significantly upregulated under 4 ℃ treatment. In summary, by conducting a genome-wide analysis of the GmftsH gene family, a strong theoretical basis is established for future studies on the functionality of GmftsH genes. This research can potentially serve as a guide for enhancing the stress tolerance characteristics of soybean.

Overexpression of VaBAM3 from Vitis amurensis enhances seedling cold tolerance by promoting soluble sugar accumulation and reactive oxygen scavenging.

The VaBAM3 cloned from Vitis amurensis can enhance the cold tolerance of overexpressed plants, but VaBAM3 knock out by CRISPR/Cas9 system weakened grape callus cold tolerance. In grape production, extreme cold conditions can seriously threaten plant survival and fruit quality. Regulation of starch content by β-amylase (BAM, EC: 3.2.1.2) contributes to cold tolerance in plants. In this study, we cloned the VaBAM3 gene from an extremely cold-tolerant grape, Vitis amurensis, and overexpressed it in tomato and Arabidopsis plants, as well as in grape callus for functional characterization. After exposure to cold stress, leaf wilting in the VaBAM3-overexpressing tomato plants was slightly less pronounced than that in wild-type tomato plants, and these plants were characterized by a significant accumulation of autophagosomes. Additionally, the VaBAM3-overexpressing Arabidopsis plants had a higher freezing tolerance than the wild-type counterparts. Under cold stress conditions, the activities of total amylase, BAM, peroxidase, superoxide dismutase, and catalase in VaBAM3-overexpressing plants were significantly higher than those in the corresponding wild-type plants. Furthermore, sucrose, glucose, and fructose contents in these lines were similarly significantly higher, whereas starch contents were reduced in comparison to the levels in the wild-type plants. Furthermore, we detected high CBF and COR gene expression levels in cold-stressed VaBAM3-overexpressing plants. Compared with those in VaBAM3-overexpressing grape callus, the aforementioned indicators tended to change in the opposite direction in grape callus with silenced VaBAM3. Collectively, our findings indicate that heterologous overexpression of VaBAM3 enhanced cold tolerance of plants by promoting the accumulation of soluble sugars and scavenging of excessive reactive oxygen species. These findings provide a theoretical basis for the cultivation of cold-resistant grape and support creation of germplasm resources for this purpose.

Investigating the Creep Damage and Permeability Evolution Mechanism of Phyllite Considering Non-Darcy’s Flow

AbstractThis study investigates the intricate interplay of hydraulic coupling, creep damage, and permeability evolution mechanisms in phyllite, focusing on their relevance to tunnel engineering design and long-term stability in soft rock formations. To achieve this, conventional triaxial tests were conducted on saturated phyllite specimens under creep-seepage acoustic emission conditions. The results were systematically analyzed to unveil the inherent characteristics of creep deformation, seepage rate evolution, and damage progression in phyllite when subjected to stress–seepage coupling. Furthermore, a permeability model for representative volume element (RVE) was developed based on meso-mechanics principles, considering the distinctive attributes of low-permeability non-Darcy’s flow in rock. Consequently, a novel relationship between effective damage and permeability was established. We determined that seepage pressure induces three key effects on the creep damage mechanism of phyllite samples: (1) It adds to the existing stress through the superposition of osmotic pressure and axial load, (2) it induces tensile expansion stress because of pore water pressure, and (3) it softens the fracture surfaces to some extent. More importantly, this study validates the relationship between effective damage and permeability through the fitting of creep and damage parameters, which were obtained from the test results, and it reveals a square relationship between subsequent damage and permeability under stress conditions. The findings of this study provide a robust theoretical foundation for comprehending the evolution of damage and permeability characteristics during the creep process of rock under seepage conditions. We obtained essential insights and quantitative analyses for comprehending the damage mechanisms in rock creep under hydraulic coupling, which has significant implications for tunnel engineering and long-term rock stability assessments in soft rock environments.

Experimental investigation on macroscopic fracture and microstructural evolution of granite under high-temperature steam action

This study conducted simulated experiments on the thermal fracturing of granite under non-uniform stress conditions using the method of injecting high-temperature and high-pressure steam. Additionally, a comprehensive investigation was carried out on the thermal fracturing characteristics of granite after steam treatment, as well as the distribution patterns of cracks. The results show that: Firstly, the process of fracturing granite using high-temperature and high-pressure steam as a medium can be divided into five stages. The threshold temperature for thermal fracturing of granite is 260 ℃, at which point a large number of cracks develop or form interconnected networks. Secondly, under high-temperature conditions, the tensile strength of granite is greatly reduced. The temperature at which brittle fracture occurs in granite during the experiment was found to be 427 ℃ at a steam pressure of 10.6 MPa. Thirdly, under the influence of high-temperature and high-pressure steam, granite not only generates primary cracks along the direction perpendicular to the minimum principal stress but also produces a certain number of secondary cracks in other directions. The crack propagation direction can be determined from the velocity and permeability distribution maps of fractured granite. Finally, the fracture surface of granite exhibits significant brittle characteristics. The fracture morphology mainly includes transgranular and intergranular fractures. In the experiment, the fracture surface also showed fragmented and fatigue-induced fracture features. The fractal dimension of micro-morphological features can be used to assess the extent of thermal fracturing at different locations on the fracture surface. Through macroscopic fracturing experiments and comprehensive analysis of theory, wave velocity, and micro-morphology, the study obtained relevant crack propagation patterns and the formation of cracks in different directions. This is of practical significance in the formation of interconnected spatial fracture networks, thereby helping to increase the thermal exchange surface area of artificial reservoirs and improve the efficiency of geothermal exploitation.

A stability-indicating HPLC assay of ten different vitamins in a food supplement: Appraisal of the method's greenness, whiteness, and blueness

Due to their susceptibility to degradation, vitamin levels in food formulations may differ from those found in the finished product. Vitamin levels can be impacted by processing and storage. In this work, the ingredients of Strong B50 ® film-coated tablets were estimated simultaneously using simple efficient stability indicating HPLC method. Strong B50 ® film-coated tablets contain thiamine (VB1), riboflavin (VB2), calcium pantothenate (VB5), pyridoxine (VB6), vitamin C (VC), folic acid (FA), biotin (BT), inositol (IS), niacin (NC), para-aminobenzoic acid (PB), cyanocobalamine (B12), choline bitartarate, and iron gluconate. Hypersil BDS C18 column was used for achieving reasonable separation. Mobile phases (A) and (B) were utilized, the mobile phase (A) consisted of 0.015 M Hexane sulfonic acid sodium salt + 0.1 % Triethylamine and orthophosphoric acid was used to adjust the pH to (2.9) while (B) system consisted of acetonitrile. Validation of the method was assessed using International Conference of Harmonization (ICH) parameters, where linearity, accuracy, selectivity, and robustness of the method were investigated. Correlations were above 0.99, accuracy results ranged from 97.6 to 102.8 % and limits for detection and quantitation (LOD and LOQ) values were determined for each vitamin in μg/mL except for FA and BT in ng/mL. LOD values were between 0.006 and 15.08 μg/mL while LOQ values ranged from 0.031 to 49.77 μg/mL. Stability studies were conducted under stressed conditions and degradation percentages were computed. Where, VB5, VB6, FA and PB, VC, and NC were the most degradable vitamins. Whiteness evaluation using the modern RGB 12 algorithm compared our method and the old reported one by Sasaki et al., 2020. The comparison favored our newly developed method in terms of analytical performance, practical applicability and greenness. Besides, AGREE and GAPI soft wares were used to assess the greenness of the method. It was clear that the results of colored pictograms confirm low hazardous impact and that the new method is greener with AGREE score of 0.66. Furthermore, the functionality and applicability of the novel HPLC approach were concluded via the Blue Applicability Grade Index (BAGI) tool with a final score of 82.5.

A novel BAG5 variant impairs the ER stress response pathway, causing dilated cardiomyopathy and arrhythmia

Pathogenic BAG5 variants recently linked to dilated cardiomyopathy (DCM) prompt further investigation into phenotypic, mutational, and pathomechanistic aspects. We explored the clinical and molecular characteristics of DCM associated with BAG5 variants, uncovering the consistently severe manifestations of the disease and its impact on the endoplasmic reticulum (ER) stress response. The analysis involved three siblings affected by DCM and arrhythmia, along with their four unaffected siblings, their unaffected father, and their mother who exhibited arrhythmia. The parents were consanguineous. Exome and Sanger sequencing identified a novel BAG5 variant, c.444_445delGA (p.Lys149AsnfsTer6), homozygous in affected siblings and heterozygous in parents and unaffected siblings. We generated heterozygous and homozygous Bag5 point mutant knock-in (KI) mice and evaluated cardiac pathophysiology under stress conditions, including tunicamycin (TN) administration. Bag5−/− mice displayed no abnormalities up to 12 months old and showed no anomalies during an exercise stress test. However, following TN injection, Bag5−/− mice exhibited significantly reduced left ventricular fractional shortening (LVFS) and ejection fraction (LVEF). Their cardiac tissues exhibited a notable increase in apoptotic cells, despite non-distinctive changes in CHOP and GRP78 levels. Interestingly, only Bag5 KI male mice demonstrated arrhythmia, which was more pronounced in Bag5−/− than in Bag5+/−males. Here, our study reveals a novel BAG5 mutation causing DCM by impairing the ER stress response, with observed sex-specific arrhythmia differences.

Effectiveness of Mindfulness-Based Interventions in Enhancing Sports Performance

Purpose: The aim of the study was to assess the effectiveness of mindfulness-based interventions in enhancing sports performance. Methodology: This study adopted a desk methodology. A desk study research design is commonly known as secondary data collection. This is basically collecting data from existing resources preferably because of its low cost advantage as compared to a field research. Our current study looked into already published studies and reports as the data was easily accessed through online journals and libraries. Findings: The study found that mindfulness-based interventions (MBIs) hold promise in enhancing sports performance across various disciplines. The study indicate that athletes who engage in mindfulness practices experience improvements in attentional control, emotional regulation, and stress management, all of which are critical for optimal athletic performance. By cultivating present-moment awareness and fostering a non-judgmental attitude toward thoughts and sensations, athletes can better focus on the task at hand, manage competitive pressure, and maintain peak performance under stressful conditions. Furthermore, MBIs have been shown to facilitate skill acquisition and execution, as well as promote recovery and injury rehabilitation. Overall, these findings highlight the potential of incorporating mindfulness training into sports training programs to enhance athletes' mental resilience and overall performance. However, further research is needed to elucidate the specific mechanisms through which mindfulness exerts its effects on sports performance and to optimize the integration of MBIs into athletic training regimens. Implications to Theory, Practice and Policy: Self-determination theory, cognitive-behavioral theory and flow theory may be used to anchor future studies on assessing effectiveness of mindfulness-based interventions in enhancing sports performance. Develop evidence-based mindfulness training programs tailored specifically for athletes across different sports disciplines and performance levels. Advocate for the integration of mindfulness training into athlete development programs at the grassroots, elite, and professional levels.

Dipeptidyl peptidase-4 disturbs adipocyte differentiation via the negative regulation of the glucagon-like peptide-1/adiponectin-cathepsin K axis in mice under chronic stress conditions.

Exposure to chronic psychosocial stress is a risk factor for metabolic disorders. Because dipeptidyl peptidase-4 (DPP4) and cysteinyl cathepsin K (CTSK) play important roles in human pathobiology, we investigated the role(s) of DPP4 in stress-related adipocyte differentiation, with a focus on the glucagon-like peptide-1 (GLP-1)/adiponectin-CTSK axis invivo and invitro. Plasma and inguinal adipose tissue from non-stress wild-type (DPP4+/+), DPP4-knockout (DPP4-/-) and CTSK-knockout (CTSK-/-) mice, and stressed DPP4+/+, DPP4-/-, CTSK-/-, and DPP4+/+ mice underwent stress exposure plus GLP-1 receptor agonist exenatide loading for 2 weeks and then were analyzed for stress-related biological and/or morphological alterations. On day 14 under chronic stress, stress decreased the weights of adipose tissue and resulted in harmful changes in the plasma levels of DPP4, GLP-1, CTSK, adiponectin, and tumor necrosis factor-α proteins and the adipose tissue levels of CTSK, preadipocyte factor-1, fatty acid binding protein-4, CCAAT/enhancer binding protein-α, GLP-1 receptor, peroxisome proliferator-activated receptor-γ, perilipin2, secreted frizzled-related protein-4, Wnt5α, Wnt11 and β-catenin proteins and/or mRNAs as well as macrophage infiltration in adipose tissue; these changes were rectified by DPP4 deletion. GLP-1 receptor activation and CTSK deletion mimic the adipose benefits of DPP4 deficiency. Invitro, CTSK silencing and overexpression respectively prevented and facilitated stress serum and oxidative stress-induced adipocyte differentiation accompanied with changes in the levels of pref-1, C/EBP-α, and PPAR-γ in 3T3-L1 cells. Thus, these findings indicated that increased DPP4 plays an essential role in stress-related adipocyte differentiation, possibly through a negative regulation of GLP-1/adiponectin-CTSK axis activation in mice under chronic stress conditions.

Metabolic and productive parameters of lactating dairy cows under heat stress conditions supplemented with plant polyphenol extract.

The impact of heat stress on dairy cattle leads to significant economic losses and a negative impact on the welfare of the animals. The objective of this research was to evaluate the effect of the nutritional additive (Thermoplus®) in dairy cows under postpartum heat stress conditions, and its effects on the metabolic profile, production and quality of milk. Eighteen lactating Holstein cows (8 multiparous and ten primiparous), in a free-stall system, with a mean body condition score (BCS) of 3.14 ± 0.05, live weight of 624.55 ± 18, 61kg, with initial mean days in milk (DIM) of 90 ± 10.11, were selected. The animals were grouped into a control (CG, n = 9) and a treatment (TG, n = 9). Both groups underwent 14days of diet adaptation, the TG received the basal diet supplemented with 50g of the additive, once a day, individually, while the control group received only the total diet. Data collection of metabolic and productive parameters were evaluated on days -14 (before adaptation), 1 (after the diet adaptation period), 16, 30, and 44. Milk, blood, and body condition score (BCS) were collected once a day, and heart rate, respiratory rate, and rectal temperature were collected twice a day. Serum concentrations of albumin, calcium, magnesium, glucose, gamma-glutamyl transferase (GGT), beta-hydroxybutyrate (BHBA), non-esterified fatty acids (NEFAs), and paraoxonase-1 (PON-1) were evaluated. In the milk, the percentage of fat, protein, lactose, and total solids were determined in each sampling. Milk yield was measured daily. Humidity and ambient temperature values were collected on the days of the collection every 30min, from 5:30 am to 5:00pm, to calculate the temperature-humidity index (THI). Statistical analyzes were performed using the SAS software (version 9.3, SAS Institute Inc., Cary, NC, USA). The THI ranged from 62.22 to 79.47. Our findings showed that when the THI was greater than 72, the animals in the TG were able to maintain milk yield (Odds ratio (OD) = -0.0577,), and the animals in the CG had a greater chance of reducing it (OD = -0.2301). Multiparous cows in the TG had higher milk yield than CG (32.57 ± 0.34 vs 30.50 ± 0.36kg per day; P = 0.0078) and lower SCC (34.110 ± 6,940 vs 665.50 ± 214.41 cells per ml; P = 0.03), with the same percentages of total solids (P > 0.05). In multiparous metabolic markers, TG when compared CG had higher albumin concentrations (2.50 ± 0.07 vs 2.12 ± 0.07g/dl; < 0.001), equal PON-1 (P > 0.05), and higher BHBA levels (0.49 ± 0.03 vs 0.39 ± 0.04mmol/l). Primiparous from the CG had higher concentrations of NEFA (0.18 ± 0.02mmol/l) than multiparous from the same group (0.09 ± 0.02mmol/l) P = 0.0265. The use of the plant polyphenol extract in postpartum Holstein cows challenged by heat stress had beneficial effects on the production and health of the mammary gland in multiparous cows without decreasing milk solids. The non-reduction of the activities of the acute phase proteins indicates an immunomodulatory and inflammatory-reducing effect of the product used.

Effect of Mycorrhizal Symbiosis on the Development of the Canary Island Tomato Variety “Manzana Negra” under Abiotic Stress Conditions

Tomato production in the Canary Islands has significantly decreased in recent years due to the presence of parasites and pathogens, poor-quality irrigation water, lack of infrastructure modernization, and increased competition. To address this issue, local varieties with better agro-climatic adaptation and organoleptic characteristics have been cultivated. These varieties show their maximum potential under an agro-ecological cultivation system, where the beneficial micro-organisms of the rhizosphere (in general) and mycorrhizal fungi (in particular) have a positive influence on their development, especially when the plants are subjected to biotic or abiotic stresses. Irrigation water in Canary Islands tomato cultivation comes from groundwater sources with moderate levels of sodium and chlorides or sodium and bicarbonates. This study evaluated the response of mycorrizal plants of the local tomato variety “Manzana Negra” under abiotic stress conditions due to the presence of chlorides and bicarbonates. Two tests were carried out with mycorrhizal and non-mycorrhizal plants. In the first one, 0, 75, and 150 mM NaCl solutions were applied. In the second, the nutrient solution was enriched with sodium bicarbonate at doses of 0, 2.5, 5, 7.5, 10, and 12.5 mM. Presence of native mycorrhizae improved the growth and nutrition of plants affected by irrigation with saline and alkaline water containing chloride and sodium carbonate. Symbiosis produced statistically significant increases in all plant-development-related variables (stem length and diameter; fresh and dry weight) in all bicarbonate concentrations. However, the results with the application of sodium chloride do not seem to indicate a positive interaction in most of the analytical parameters at 150 mM NaCl concentration. The mycorrhizal inoculation with local fungi can be interesting in the production of seedlings of this tomato variety in situations of moderate salinity, especially under bicarbonate stress conditions.

Operational nursing managers' experiences of clinical supervision at a Johannesburg Hospital.

Clinical supervision is pivotal in supporting nurses in rendering quality, safe patient care. Therefore, it is essential to understand clinical supervision from operational nursing managers' context to define existing challenges and propose suitable recommendations. This study aimed to explore and describe operational nursing managers' experiences of clinical supervision within the context of an academic hospital in Gauteng province and propose evidence-based practice recommendations to improve patient safety and the quality of clinical supervision. An exploratory, sequential, mixed-method design was used and implemented over two phases to take advantage of the strengths of both the qualitative and quantitative research designs. Unstructured individual interviews were conducted to collect data in phase one, and an adapted Manchester Clinical Supervision Scale (MCSS) questionnaire was used to collect data in phase two. Operational nursing managers work in stressful conditions and environments with a gross shortage of staff and tools of the trade while being expected to deliver high-quality and safe nursing care. Of the sampled respondents, 36% (n= 17) were dissatisfied with the supervision they received, while 64% (n= 30) were indifferent in the sense that they did not think it was adequate or inadequate. Clinical supervisors should be trained and supported in clinical supervision, with regular workshops on interpersonal relations.Contribution:A clearer understanding of clinical supervision within the hospital context and evidence-based practice recommendations to improve patient safety and the quality of clinical supervision.

MCT1-mediated transport of valeric acid promotes porcine preimplantation embryo development by improving mitochondrial function and inhibiting the autophagic AMPK-ULK1 pathway

Oocytes and embryos are highly sensitive to environmental stress in vivo and in vitro. During in vitro culture, many stressful conditions can affect embryo quality and viability, leading to adverse clinical outcomes such as abortion and congenital abnormalities. In this study, we found that valeric acid (VA) increased the mitochondrial membrane potential and ATP content, decreased the level of reactive oxygen species that the mitochondria generate, and thus improved mitochondrial function during early embryonic development in pigs. VA decreased expression of the autophagy-related factors LC3B and BECLIN1. Interestingly, VA inhibited expression of autophagy-associated phosphorylation-adenosine monophosphate-activated protein kinase (p-AMPK), phosphorylation-UNC-51-like autophagy-activated kinase 1 (p-ULK1, Ser555), and ATG13, which reduced apoptosis. Short-chain fatty acids (SCFAs) can signal through G-protein-coupled receptors on the cell membrane or enter the cell directly through transporters. We further show that the monocarboxylate transporter 1 (MCT1) was necessary for the effects of VA on embryo quality, which provides a new molecular perspective of the pathway by which SCFAs affect embryos. Importantly, VA significantly inhibited the AMPK-ULK1 autophagic signaling pathway through MCT1, decreased apoptosis, increased expression of embryonic pluripotency genes, and improved embryo quality.

Analytical Ultracentrifugation to Assess the Quality of LNP-mRNA Therapeutics.

The approval of safe and effective LNP-mRNA vaccines during the SARS-CoV-2 pandemic is catalyzing the development of the next generation of mRNA therapeutics. Proper characterization methods are crucial for assessing the quality and efficacy of these complex formulations. Here, we show that analytical ultracentrifugation (AUC) can measure, simultaneously and without any sample preparation step, the sedimentation coefficients of both the LNP-mRNA formulation and the mRNA molecules. This allows measuring several quality attributes, such as particle size distribution, encapsulation efficiency and density of the formulation. The technique can also be applied to study the stability of the formulation under stress conditions and different buffers.

Environment modulates protein heterogeneity through transcriptional and translational stop codon readthrough

Stop codon readthrough events give rise to longer proteins, which may alter the protein’s function, thereby generating short-lasting phenotypic variability from a single gene. In order to systematically assess the frequency and origin of stop codon readthrough events, we designed a library of reporters. We introduced premature stop codons into mScarlet, which enabled high-throughput quantification of protein synthesis termination errors in E. coli using fluorescent microscopy. We found that under stress conditions, stop codon readthrough may occur at rates as high as 80%, depending on the nucleotide context, suggesting that evolution frequently samples stop codon readthrough events. The analysis of selected reporters by mass spectrometry and RNA-seq showed that not only translation but also transcription errors contribute to stop codon readthrough. The RNA polymerase was more likely to misincorporate a nucleotide at premature stop codons. Proteome-wide detection of stop codon readthrough by mass spectrometry revealed that temperature regulated the expression of cryptic sequences generated by stop codon readthrough in E. coli. Overall, our findings suggest that the environment affects the accuracy of protein production, which increases protein heterogeneity when the organisms need to adapt to new conditions.

Development and characterization of a coaxial strain-sensing cable integrated steel strand for wide-range stress monitoring

Abstract Monitoring the stress of steel strands, from initial tension to eventual failure, is paramount for assessing structural safety and understanding its failure mechanism. Current monitoring methods are restricted in measuring stress only until yielding because of their limited range. This study proposes a novel coaxial strain-sensing cable (CSSC) based intelligent steel strand (CSSC-ISS), which has both functions of force-bearing and self-sensing. First, the prototype design of CSSC-ISS and the sensing principle of CSSC are introduced. Then, a fabrication method of small-diameter CSSC is proposed, which is then encapsulated with glass fiber reinforced polymer (GFRP) material, forming a GFRP sensing rod (GFRP-SR). The next step involves replacing the strand’s central wire with the GFRP-SR, culminating in the creation of the CSSC-ISS. Finally, Laboratory tests show that the CSSC has excellent strain-sensing performance with a resolution of at least 100 µε and a measurement range of 150,000 µε. The GFRP-SR offers good sensing potential and comparable mechanical strength to standard GFRP rods. Notably, the CSSC-ISS could measure stress up to strand failure, retaining 87.9% tensile strength and 88.7% elastic modulus compared to standard steel strands. It is verified that the CSSC-ISS can consistently measure its stress condition throughout its life cycle without compromising its load-bearing potential.

Phosphorylation of phospholamban promotes SERCA2a activation by dwarf open reading frame (DWORF)

In cardiac myocytes, the type 2a sarco/endoplasmic reticulum Ca-ATPase (SERCA2a) plays a key role in intracellular Ca regulation. Due to its critical role in heart function, SERCA2a activity is tightly regulated by different mechanisms, including micropeptides. While phospholamban (PLB) is a well-known SERCA2a inhibitor, dwarf open reading frame (DWORF) is a recently identified SERCA2a activator. Since PLB phosphorylation is the most recognized mechanism of SERCA2a activation during adrenergic stress, we studied whether PLB phosphorylation also affects SERCA2a regulation by DWORF. By using confocal Ca imaging in a HEK293 expressing cell system, we analyzed the effect of the co-expression of PLB and DWORF using a bicistronic construct on SERCA2a-mediated Ca uptake. Under these conditions of matched expression of PLB and DWORF, we found that SERCA2a inhibition by non-phosphorylated PLB prevails over DWORF activating effect. However, when PLB is phosphorylated at PKA and CaMKII sites, not only PLB's inhibitory effect was relieved, but SERCA2a was effectively activated by DWORF. Förster resonance energy transfer (FRET) analysis between SERCA2a and DWORF showed that DWORF has a higher relative affinity for SERCA2a when PLB is phosphorylated. Thus, SERCA2a regulation by DWORF responds to the PLB phosphorylation status, suggesting that DWORF might contribute to SERCA2a activation during conditions of adrenergic stress.

Genome-wide identification and analysis of the cotton ALDH gene family

BackgroundAldehyde dehydrogenases (ALDHs) are a family of enzymes that catalyze the oxidation of aldehyde molecules into the corresponding carboxylic acid, regulate the balance of aldehydes and protect plants from the poisoning caused by excessive accumulation of aldehydes; however, this gene family has rarely been studied in cotton.ResultsIn the present study, genome-wide identification was performed, and a total of 114 ALDH family members were found in three cotton species, Gossypium hirsutum, Gossypium arboreum and Gossypium raimondii. The ALDH genes were divided into six subgroups by evolutionary analysis. ALDH genes in the same subgroup showed similar gene structures and conserved motifs, but some genes showed significant differences, which may result in functional differences. Chromosomal location analysis and selective pressure analysis revealed that the ALDH gene family had experienced many fragment duplication events. Cis-acting element analysis revealed that this gene family may be involved in the response to various biotic and abiotic stresses. The RT‒qPCR results showed that the expression levels of some members of this gene family were significantly increased under salt stress conditions. Gohir.A11G040800 and Gohir.D06G046200 were subjected to virus-induced gene silencing (VIGS) experiments, and the sensitivity of the silenced plants to salt stress was significantly greater than that of the negative control plants, suggesting that Gohir.A11G040800 and Gohir.D06G046200 may be involved in the response of cotton to salt stress.ConclusionsIn total, 114 ALDH genes were identified in three Gossypium species by a series of bioinformatics analysis. Gene silencing of the ALDH genes of G. hirsutum revealed that ALDH plays an important role in the response of cotton to salt stress.

A New Remote Sensing Index for Forest Dryness Monitoring Using Multi-Spectral Satellite Imagery

Canopy water content is a fundamental indicator for assessing the level of plant water stress. The correlation between changes in water content and the spectral reflectance of plant leaves at near-infrared (NIR) and short-wave infrared (SWIR) wavelengths forms the foundation for developing a new remote sensing index, the Infrared Canopy Dryness Index (ICDI), to monitor forest dryness that can be used to predict the consequences of water stress. This study introduces the index, that uses spectral reflectance analysis at near-infrared wavelengths, encapsulated by the Normalized Difference Infrared Index (NDII), in conjunction with specific canopy conditions as depicted by the widely recognized Normalized Difference Vegetation Index (NDVI). Development of the ICDI commenced with the construction of an NDII/NDVI feature space, inspired by a conceptual trapezoid model. This feature space was then parameterized, and the spatial region indicative of water stress conditions, referred to as the dry edge, was identified based on the analysis of 10,000 random observations. The ICDI was produced from the combination of the vertical distance (i.e., under consistent NDVI conditions) from an examined observation to the dry edge. Comparisons between data from drought-affected and non-drought-affected control plots in the Australian Northern Jarrah Forest affirmed that the ICDI effectively depicted forest dryness. Moreover, the index was able to detect incipient water stress several months before damage from an extended drought and heatwave. Using freely available satellite data, the index has potential for broad application in monitoring the onset of forest dryness. This will require validation of the ICDI in diverse forest systems to quantify the efficacy of the index.

The role and mechanism of Bacillus megaterium strain A14 in inhibiting the cadmium uptake by peanut plants in acidic red soil.

This study explores the potential of Cd-resistant bacteria, specifically Bacillus megaterium A14, to decrease Cd accumulation in peanuts, a crop susceptible to metal uptake from contaminated soils, by understanding the bacterium's impact on plant Cd absorption mechanisms. Through pot experiments, we observed that A14 inoculation significantly increased peanut biomass under Cd stress conditions, primarily by immobilizing the metal and reducing its bioavailability. The bacterium effectively changed the distribution of Cd, with a notable 46.53% reduction in the exchangeable fraction, which in turn limited the expression of genes related to Cd transport in peanuts. Additionally, A14 enhanced the plant's antioxidant response, improving its tolerance to stress. Microbial analysis through 16S sequencing demonstrated that A14 inoculation altered the peanut rhizosphere, particularly by increasing populations of Firmicutes and Proteobacteria, which play crucial roles in soil remediation from heavy metals. The A14 strain effectively counters Cd toxicity in peanuts, promoting growth through soil Cd sequestration, root barrier biofilm formation, antioxidant system enhancement, suppression of Cd transport genes, and facilitation of Cd-remediating microorganisms.

Resilient Response to Combined Heat and Drought Stress Conditions of a Tomato Germplasm Collection, Including Natural and Ethyl Methanesulfonate-Induced Variants

Agricultural systems are currently facing significant issues, primarily due to population growth rates in the context of global climate change. Rising temperatures cause plant heat stress and impact crop yield, which in turn compromises global food production and safety. Climate change is also having a significant impact on water availability around the world, and droughts are becoming more frequent and severe in many regions. The combined effect of both heat and drought stresses increases plant damage, resulting in reduced plant development and productivity loss. Therefore, developing heat–drought-tolerant crop varieties is crucial for enhancing yield under these challenging conditions. Tomato (Solanum lycopersicum L.), a major vegetable crop highly appreciated for its nutritional qualities, is particularly sensitive to extreme temperatures, which have a significant negative impact on tomato fruit setting and cause male gametophyte abortion. In this work, a classical genetic approach was employed to identify tomato genotypes showing a resilient response to combined heat and drought stress conditions. A phenotype screening of a natural germplasm collection and an ethyl methanesulfonate (EMS) mutagenized population resulted in the identification of a significant number of tomato lines tolerant to combined heat and drought conditions, specifically 161 EMS lines and 24 natural accessions as tolerant. In addition, TILLING and Eco-TILLING analyses were used as proof-of-concept to isolate new genetic variants of genes previously reported as key regulators of abiotic stress responses in different species. The identification of these variants holds the potential to provide suitable plant material for breeding programs focused on enhancing tomato resilience to adverse climate conditions.